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The map label for this gene is lptD [H]

Identifier: 75675871

GI number: 75675871

Start: 1830344

End: 1832833

Strand: Direct

Name: lptD [H]

Synonym: Nwi_1679

Alternate gene names: 75675871

Gene position: 1830344-1832833 (Clockwise)

Preceding gene: 75675870

Following gene: 75675872

Centisome position: 53.8

GC content: 61.81

Gene sequence:

>2490_bases
GTGGCCCCAATCGCCACCCTCCGTAAACGATCGGCTATTCGCGAGCGGCGCAGCCCCCTGCGCTGCTATCGGTCGGGTGC
GTCCGTGATTCCGGCCTCGCTGACGGCGGCCGTTCTCGGTCTGATCGCCGCCGCCGTGATGGACGTCGTCGCGACCACTC
CGGCTGCCGCGCAAAGCTTCACCTACAACCCTCGCCCACCCAAGCCCGCGCCGCCGCCAAGGCAGAACGATGGGCAGATG
CTCGTTCAGGCCACCGAGCTTGACTACGACTACAATAATTCCCGCGTGTCAGCCGTCGGCAACGTGCAGATGTTCTACAA
CGGCACGAGCGTCGAGGCGGATCGGGTCATCTACGATCAGAAAACCAAGCGGCTCCATGCCGAAGGCAACATCCGCATGA
CCGATGCGGACGGCAAGGTCACCTATGCCAACATTCTCGATCTGAGCGACGACTACCGGGACGGATTCGTTGATTCGCTG
AGGGTCGACACCGCCGACGCGACGCGCATGGCCGCCACCCGCGCCGATCGGACCGAAGGCAACTACACGGTGTTCGAAAA
CGGCGTCTATACGGCCTGCGCGCCATGCAAGGAGAACCCGAAGAAACCCCCGCTATGGCAGGTCAAGGGCGCCCGGATCA
TTCATAACCAGACCGAGAAGATGCTGTACTTCGAGGACGCCCGTTTGGAATTCTATGGCGTGCCGATGGCTTACATGCCC
TTCTTCTCGACACCCGATCCGACGGTGAAGCGCAAGAGCGGCTTCCTGATGCCGGCCGTGACCGCCTTTACCGGTTTCGG
GGTCGGGGTCGAGACTCCCTACTATTTCGCGTTGGCGCCGAACTATGACCTGACCATCTCTCCCCGGTTCACCACGCGGC
AAGGCGTATTGATGCATGGCGAGTTCCGGCAGCGTTTCGAGGACGGCGCCTATCAGATCCGTGCCTATGGCATCAATCAG
CTTGATCCGGACGCCTTCGGCGCAGGTCCCGGCAACCGGGACTTCCGGGGCGGTGTCGATACCAAGGGCCAGTTTGCCTT
GAACGACAAATGGATCTGGGGTTGGGACGGCGTCCTGCTGAGCGACTACTTCTTCTTCTCCGACTATCGGTTGAGGCAGT
ACAGGGACCCGATGCAATCGTTCCTGACCTTGCCGACCGAAGCGCTCTCGCAGCTCTATCTGACCGGCGTCGGCAACCGC
AGCTACTTCGACGCCCGCGCCGTCCACTACCTGAGCTTCTCCGGCAATCAGAGCCTGGTTCCGGTGATCGCGCCCGTGGT
TGATTACTCGAACGTCATCAACCATTCGGTGATCGGCGGCGAGTTCAGCTACAAATTCAATTTCGTCAATCTCATGCGCG
ATCAGCCGCAGTTCGATCCGATCTCGACACTGGCGAACACCTCCTTCGTGTGCGGGCCGACATCGGCTGACCCGGCGGCG
AGACCGCGGGATCTCTGTCTGCTTCGAGGCATTCCGGGAACCTATACGCGTCTGACGGCGCAGGCTCAGTGGCGGAGGTC
GTTCACGGATTCCTTCGGTCAGATATGGACGCCGTTTGCCTCGCTTCGCGCCGACGCCATCAATGCCGACATTTCGAACC
AGCCGGGCGTATCCGAGTTCCTCCAGCCCGGCGCCACGCAGACAGTCCGCCTCATGCCGGCGGTGGGCCTCGAATATCGC
TATCCCTTCATCAACGTTCAGCCCTGGGGCACCACGACGCTCGAACCGATCGCGCAGGTTATCATCCGCCCGAACGAGAG
CTATGCGGGCCGGCTGCCGAACGAGGATGCGCAGAGCCTGGTCTTCGACGCCACGAACCTGTTCCGGGTGGACAAGTTCT
CCGGTTACGACCGTGTCGAAGGCGGTGGCCGCGCCAATGTGGGCGTGCAGGCGACGACTCAATTCGACCGCGGCGGCGCG
ATCAATTTTCTGTTCGGACAGTCCTATCACCTGTTCGGCCTCAACTCGTTCGCCGTCGCGGACATGACCAACACCGGTGT
CAATTCCGGCTTGCAGCGCGCGCAGTCAGACTACGTGACAAGCGTCAATTATTCGCCGAACAGCGCCTTCACGTTCAGCA
CCCGCGCGCGCATTGATGAGACGACCCTGAATGTGCAGCGCTTCGAAGCCGAAGGCCGCGCCAGTTTCAACCGCTGGTCC
GGGAGCATTTTGTACGGCGATTACGCGGAGCAGCCCAACCTCGGGTTCCTGACAAGGCGCCGGGGTATCCTGGGCTCCGG
CTCCATAAAGATCGCATCCAACTGGGTTGTGCAGGGTGCGGCGCGCTGGGATCTGGAAGCCAGACAGATCAACCAGTATG
CGGTCGGCGCGGGATACGTGGACGATTGTTTCGTGCTCGGCGTCAACTACGTGACATCATACATTTACGCTGCGGGAGCA
GCGCCGCCCCAGCTTAACCACGCTTTCATGCTTCAGCTTGGGCTGAGAACCCTGGGGCAGACCCGCCAAGGGTCGACCGG
CGTCCGCTAG

Upstream 100 bases:

>100_bases
GCAAGGCCGAATTGATGAATCCAATCGCCGCGGCATGGCTGCCGGTTTGCGTCGGCGGCCTTACCGGTTTTTTGGTCTTG
CTGCACCAGGAGGACGGATA

Downstream 100 bases:

>100_bases
AGTCGTTCACCGCTTCATGGAAGCGATGAACGACTCTAGCCTTTTGATTGACGCGTTTTCTTTACGCGAACCGGATTCCA
CTTCGCTCGAAAACGCTATA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 829; Mature: 828

Protein sequence:

>829_residues
MAPIATLRKRSAIRERRSPLRCYRSGASVIPASLTAAVLGLIAAAVMDVVATTPAAAQSFTYNPRPPKPAPPPRQNDGQM
LVQATELDYDYNNSRVSAVGNVQMFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKVTYANILDLSDDYRDGFVDSL
RVDTADATRMAATRADRTEGNYTVFENGVYTACAPCKENPKKPPLWQVKGARIIHNQTEKMLYFEDARLEFYGVPMAYMP
FFSTPDPTVKRKSGFLMPAVTAFTGFGVGVETPYYFALAPNYDLTISPRFTTRQGVLMHGEFRQRFEDGAYQIRAYGINQ
LDPDAFGAGPGNRDFRGGVDTKGQFALNDKWIWGWDGVLLSDYFFFSDYRLRQYRDPMQSFLTLPTEALSQLYLTGVGNR
SYFDARAVHYLSFSGNQSLVPVIAPVVDYSNVINHSVIGGEFSYKFNFVNLMRDQPQFDPISTLANTSFVCGPTSADPAA
RPRDLCLLRGIPGTYTRLTAQAQWRRSFTDSFGQIWTPFASLRADAINADISNQPGVSEFLQPGATQTVRLMPAVGLEYR
YPFINVQPWGTTTLEPIAQVIIRPNESYAGRLPNEDAQSLVFDATNLFRVDKFSGYDRVEGGGRANVGVQATTQFDRGGA
INFLFGQSYHLFGLNSFAVADMTNTGVNSGLQRAQSDYVTSVNYSPNSAFTFSTRARIDETTLNVQRFEAEGRASFNRWS
GSILYGDYAEQPNLGFLTRRRGILGSGSIKIASNWVVQGAARWDLEARQINQYAVGAGYVDDCFVLGVNYVTSYIYAAGA
APPQLNHAFMLQLGLRTLGQTRQGSTGVR

Sequences:

>Translated_829_residues
MAPIATLRKRSAIRERRSPLRCYRSGASVIPASLTAAVLGLIAAAVMDVVATTPAAAQSFTYNPRPPKPAPPPRQNDGQM
LVQATELDYDYNNSRVSAVGNVQMFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKVTYANILDLSDDYRDGFVDSL
RVDTADATRMAATRADRTEGNYTVFENGVYTACAPCKENPKKPPLWQVKGARIIHNQTEKMLYFEDARLEFYGVPMAYMP
FFSTPDPTVKRKSGFLMPAVTAFTGFGVGVETPYYFALAPNYDLTISPRFTTRQGVLMHGEFRQRFEDGAYQIRAYGINQ
LDPDAFGAGPGNRDFRGGVDTKGQFALNDKWIWGWDGVLLSDYFFFSDYRLRQYRDPMQSFLTLPTEALSQLYLTGVGNR
SYFDARAVHYLSFSGNQSLVPVIAPVVDYSNVINHSVIGGEFSYKFNFVNLMRDQPQFDPISTLANTSFVCGPTSADPAA
RPRDLCLLRGIPGTYTRLTAQAQWRRSFTDSFGQIWTPFASLRADAINADISNQPGVSEFLQPGATQTVRLMPAVGLEYR
YPFINVQPWGTTTLEPIAQVIIRPNESYAGRLPNEDAQSLVFDATNLFRVDKFSGYDRVEGGGRANVGVQATTQFDRGGA
INFLFGQSYHLFGLNSFAVADMTNTGVNSGLQRAQSDYVTSVNYSPNSAFTFSTRARIDETTLNVQRFEAEGRASFNRWS
GSILYGDYAEQPNLGFLTRRRGILGSGSIKIASNWVVQGAARWDLEARQINQYAVGAGYVDDCFVLGVNYVTSYIYAAGA
APPQLNHAFMLQLGLRTLGQTRQGSTGVR
>Mature_828_residues
APIATLRKRSAIRERRSPLRCYRSGASVIPASLTAAVLGLIAAAVMDVVATTPAAAQSFTYNPRPPKPAPPPRQNDGQML
VQATELDYDYNNSRVSAVGNVQMFYNGTSVEADRVIYDQKTKRLHAEGNIRMTDADGKVTYANILDLSDDYRDGFVDSLR
VDTADATRMAATRADRTEGNYTVFENGVYTACAPCKENPKKPPLWQVKGARIIHNQTEKMLYFEDARLEFYGVPMAYMPF
FSTPDPTVKRKSGFLMPAVTAFTGFGVGVETPYYFALAPNYDLTISPRFTTRQGVLMHGEFRQRFEDGAYQIRAYGINQL
DPDAFGAGPGNRDFRGGVDTKGQFALNDKWIWGWDGVLLSDYFFFSDYRLRQYRDPMQSFLTLPTEALSQLYLTGVGNRS
YFDARAVHYLSFSGNQSLVPVIAPVVDYSNVINHSVIGGEFSYKFNFVNLMRDQPQFDPISTLANTSFVCGPTSADPAAR
PRDLCLLRGIPGTYTRLTAQAQWRRSFTDSFGQIWTPFASLRADAINADISNQPGVSEFLQPGATQTVRLMPAVGLEYRY
PFINVQPWGTTTLEPIAQVIIRPNESYAGRLPNEDAQSLVFDATNLFRVDKFSGYDRVEGGGRANVGVQATTQFDRGGAI
NFLFGQSYHLFGLNSFAVADMTNTGVNSGLQRAQSDYVTSVNYSPNSAFTFSTRARIDETTLNVQRFEAEGRASFNRWSG
SILYGDYAEQPNLGFLTRRRGILGSGSIKIASNWVVQGAARWDLEARQINQYAVGAGYVDDCFVLGVNYVTSYIYAAGAA
PPQLNHAFMLQLGLRTLGQTRQGSTGVR

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=278, Percent_Identity=29.8561151079137, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 91870; Mature: 91739

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPIATLRKRSAIRERRSPLRCYRSGASVIPASLTAAVLGLIAAAVMDVVATTPAAAQSF
CCCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
TYNPRPPKPAPPPRQNDGQMLVQATELDYDYNNSRVSAVGNVQMFYNGTSVEADRVIYDQ
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEEECCCCCCCCCEEECC
KTKRLHAEGNIRMTDADGKVTYANILDLSDDYRDGFVDSLRVDTADATRMAATRADRTEG
HHHEEECCCCEEEECCCCCEEEEEEECCCCCHHCCCHHHEEECCCHHHHHHHHHCCCCCC
NYTVFENGVYTACAPCKENPKKPPLWQVKGARIIHNQTEKMLYFEDARLEFYGVPMAYMP
CEEEEECCCEEEECCCCCCCCCCCCEEECCCEEEECCCCEEEEEECCCEEEEECCHHHCC
FFSTPDPTVKRKSGFLMPAVTAFTGFGVGVETPYYFALAPNYDLTISPRFTTRQGVLMHG
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCEEEEC
EFRQRFEDGAYQIRAYGINQLDPDAFGAGPGNRDFRGGVDTKGQFALNDKWIWGWDGVLL
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCHHH
SDYFFFSDYRLRQYRDPMQSFLTLPTEALSQLYLTGVGNRSYFDARAVHYLSFSGNQSLV
HHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCCEECCEEEEEEEECCCCCCH
PVIAPVVDYSNVINHSVIGGEFSYKFNFVNLMRDQPQFDPISTLANTSFVCGPTSADPAA
HHHHHHHHHHHHHCCCEECCEEEEEEEEEEHHCCCCCCCHHHHHHCCCEEECCCCCCCCC
RPRDLCLLRGIPGTYTRLTAQAQWRRSFTDSFGQIWTPFASLRADAINADISNQPGVSEF
CCCCEEEEECCCCCHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHH
LQPGATQTVRLMPAVGLEYRYPFINVQPWGTTTLEPIAQVIIRPNESYAGRLPNEDAQSL
HCCCCCCEEEEEECCCCEEECCEEEECCCCCCHHHHHHHHEECCCCHHCCCCCCHHHHHH
VFDATNLFRVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINFLFGQSYHLFGLNSFAVA
HHCCHHEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCEEEEEECCCEEEE
DMTNTGVNSGLQRAQSDYVTSVNYSPNSAFTFSTRARIDETTLNVQRFEAEGRASFNRWS
ECCCCCHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCHHEEEEEEECCCCCCCCCCCC
GSILYGDYAEQPNLGFLTRRRGILGSGSIKIASNWVVQGAARWDLEARQINQYAVGAGYV
CEEEECCCCCCCCCCCHHHCCCCCCCCCEEEEECEEEECCHHCCCHHHHHCCEEECCCCH
DDCFVLGVNYVTSYIYAAGAAPPQLNHAFMLQLGLRTLGQTRQGSTGVR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
APIATLRKRSAIRERRSPLRCYRSGASVIPASLTAAVLGLIAAAVMDVVATTPAAAQSF
CCHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
TYNPRPPKPAPPPRQNDGQMLVQATELDYDYNNSRVSAVGNVQMFYNGTSVEADRVIYDQ
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEEEEEECCCCCCCCCEEECC
KTKRLHAEGNIRMTDADGKVTYANILDLSDDYRDGFVDSLRVDTADATRMAATRADRTEG
HHHEEECCCCEEEECCCCCEEEEEEECCCCCHHCCCHHHEEECCCHHHHHHHHHCCCCCC
NYTVFENGVYTACAPCKENPKKPPLWQVKGARIIHNQTEKMLYFEDARLEFYGVPMAYMP
CEEEEECCCEEEECCCCCCCCCCCCEEECCCEEEECCCCEEEEEECCCEEEEECCHHHCC
FFSTPDPTVKRKSGFLMPAVTAFTGFGVGVETPYYFALAPNYDLTISPRFTTRQGVLMHG
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCCCCCEEEEC
EFRQRFEDGAYQIRAYGINQLDPDAFGAGPGNRDFRGGVDTKGQFALNDKWIWGWDGVLL
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCHHH
SDYFFFSDYRLRQYRDPMQSFLTLPTEALSQLYLTGVGNRSYFDARAVHYLSFSGNQSLV
HHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHEEEEECCCCCEECCEEEEEEEECCCCCCH
PVIAPVVDYSNVINHSVIGGEFSYKFNFVNLMRDQPQFDPISTLANTSFVCGPTSADPAA
HHHHHHHHHHHHHCCCEECCEEEEEEEEEEHHCCCCCCCHHHHHHCCCEEECCCCCCCCC
RPRDLCLLRGIPGTYTRLTAQAQWRRSFTDSFGQIWTPFASLRADAINADISNQPGVSEF
CCCCEEEEECCCCCHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHH
LQPGATQTVRLMPAVGLEYRYPFINVQPWGTTTLEPIAQVIIRPNESYAGRLPNEDAQSL
HCCCCCCEEEEEECCCCEEECCEEEECCCCCCHHHHHHHHEECCCCHHCCCCCCHHHHHH
VFDATNLFRVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINFLFGQSYHLFGLNSFAVA
HHCCHHEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCEEEEEECCCEEEE
DMTNTGVNSGLQRAQSDYVTSVNYSPNSAFTFSTRARIDETTLNVQRFEAEGRASFNRWS
ECCCCCHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCHHEEEEEEECCCCCCCCCCCC
GSILYGDYAEQPNLGFLTRRRGILGSGSIKIASNWVVQGAARWDLEARQINQYAVGAGYV
CEEEECCCCCCCCCCCHHHCCCCCCCCCEEEEECEEEECCHHCCCHHHHHCCEEECCCCH
DDCFVLGVNYVTSYIYAAGAAPPQLNHAFMLQLGLRTLGQTRQGSTGVR
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA