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The map label for this gene is ntrB [H]

Identifier: 75675636

GI number: 75675636

Start: 1572235

End: 1573410

Strand: Direct

Name: ntrB [H]

Synonym: Nwi_1444

Alternate gene names: 75675636

Gene position: 1572235-1573410 (Clockwise)

Preceding gene: 75675635

Following gene: 75675637

Centisome position: 46.21

GC content: 62.84

Gene sequence:

>1176_bases
ATGACGTCGATCGCGCAGCGTCCGCGCGTCGCGTCTTCCGATGGCGAGGCCATTCTCAACGCGCTCCCTAATCCTGTGCT
GCTGGTCGCGCCCGACGGCCGGATCGTTGACGCCAACATGGCGGCAGAGTCCTTCTTTGAGATGTCGAAGCAGTTCCTGC
AGCGCGAGCCACTGAAACAGCTTATCCCATTCGGCAGTCCGCTCCTGGCCCTGATCGATCAGGTTCGCGACAGCGGCGCG
CCCGTCAACGAGTTCAGGGTTGATCTCGGCACGCCGAGGATCGGCCGTGACCGTCAGGTCGACCTGCATGTCGCGCCCCT
GACCGAACGGCCCGCTCACGTCGTGGTGATGATCCAGGAGCGCACCATCGCCGACAAGATGGATCGGCAGTTGACCCATC
GCAGCGCGGCCCGATCTGTGATCGCGCTTGCGGCCATGCTCGCGCACGAAATCAAGAACCCGCTTTCCGGGATTCGTGGT
GCGGCGCAGCTTCTTGAACAGGGGGCGACGCCGGACGATCGAATGCTGACCCGGCTGATCTGCGACGAGGCGGACCGTAT
CGTCACGCTGGTCGATCGCATGGAGGTATTCGGCGACGAGCGGCCGATCAGGCGGGAGCCTGTCAACATTCACGCGGTGC
TCGATCATGTCAAACGGCTCGCGCAGTCGGGCTTCGCGCGAAATGTCAGGTTCACCGTTGATTACGATCCGTCGCTTCCG
CCGGTTCTCGGCGATCGGGATCAACTGGTTCAAATCTTTCTGAACCTTGTCAAGAATGCCGCCGAAGCTGTTTCCGATCT
CGGAGAAGAGGGAGAAATCCAGTTCACGACCGCATTCCGTCCCGGCTTGCGCCTTTCCCTCCCGGGCAAGAAAACGCGGG
TTTCGCTGCCGCTGGAGTTCTGCGTCAAGGACAATGGTCCCGGCGTGGCGGAGGATCTGCTTCCGAACCTGTTCGATCCG
TTCGTCACCACCAAGCAGACCGGCAGCGGGCTCGGTCTGGCGCTGGTCGCAAAGATCGTCGGGGATCACGGCGGCATCGT
CGAGTGCGAGTCGCAGCCGCGCAAGACCAACTTCCGTATCCTGCTGCCGATGTTCGATTCCGGTCCGGTACACCGGCAGG
GCGATGACGCTGCCGCGTCCGGAATACCGCCACGCACCGCGCGTCATCCAGCATGA

Upstream 100 bases:

>100_bases
AGAAATCGTGGCGGACGAAAATTCTGACGGCCGATGATCCCTCCGGCGTACAGCGGTTACTGGTGGACGCGTTCGACGAT
TTCGCGTGGAGGGCCGTGGC

Downstream 100 bases:

>100_bases
GGACAAAATGTCTCCAGCCAACATACTTGTCGCCGATGATGACGGAGCCATCCGCACGGTGATCAATCAGGCGCTGTCGC
GTGCCGGTTATGACGTGCGC

Product: nitrogen regulation protein ntrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTSIAQRPRVASSDGEAILNALPNPVLLVAPDGRIVDANMAAESFFEMSKQFLQREPLKQLIPFGSPLLALIDQVRDSGA
PVNEFRVDLGTPRIGRDRQVDLHVAPLTERPAHVVVMIQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQGATPDDRMLTRLICDEADRIVTLVDRMEVFGDERPIRREPVNIHAVLDHVKRLAQSGFARNVRFTVDYDPSLP
PVLGDRDQLVQIFLNLVKNAAEAVSDLGEEGEIQFTTAFRPGLRLSLPGKKTRVSLPLEFCVKDNGPGVAEDLLPNLFDP
FVTTKQTGSGLGLALVAKIVGDHGGIVECESQPRKTNFRILLPMFDSGPVHRQGDDAAASGIPPRTARHPA

Sequences:

>Translated_391_residues
MTSIAQRPRVASSDGEAILNALPNPVLLVAPDGRIVDANMAAESFFEMSKQFLQREPLKQLIPFGSPLLALIDQVRDSGA
PVNEFRVDLGTPRIGRDRQVDLHVAPLTERPAHVVVMIQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRG
AAQLLEQGATPDDRMLTRLICDEADRIVTLVDRMEVFGDERPIRREPVNIHAVLDHVKRLAQSGFARNVRFTVDYDPSLP
PVLGDRDQLVQIFLNLVKNAAEAVSDLGEEGEIQFTTAFRPGLRLSLPGKKTRVSLPLEFCVKDNGPGVAEDLLPNLFDP
FVTTKQTGSGLGLALVAKIVGDHGGIVECESQPRKTNFRILLPMFDSGPVHRQGDDAAASGIPPRTARHPA
>Mature_390_residues
TSIAQRPRVASSDGEAILNALPNPVLLVAPDGRIVDANMAAESFFEMSKQFLQREPLKQLIPFGSPLLALIDQVRDSGAP
VNEFRVDLGTPRIGRDRQVDLHVAPLTERPAHVVVMIQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGA
AQLLEQGATPDDRMLTRLICDEADRIVTLVDRMEVFGDERPIRREPVNIHAVLDHVKRLAQSGFARNVRFTVDYDPSLPP
VLGDRDQLVQIFLNLVKNAAEAVSDLGEEGEIQFTTAFRPGLRLSLPGKKTRVSLPLEFCVKDNGPGVAEDLLPNLFDPF
VTTKQTGSGLGLALVAKIVGDHGGIVECESQPRKTNFRILLPMFDSGPVHRQGDDAAASGIPPRTARHPA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=359, Percent_Identity=35.6545961002786, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788549, Length=379, Percent_Identity=26.6490765171504, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=33.1858407079646, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786600, Length=358, Percent_Identity=25.4189944134078, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789149, Length=235, Percent_Identity=27.2340425531915, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081816, Length=262, Percent_Identity=27.0992366412214, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=27.4336283185841, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790567, Length=359, Percent_Identity=25.6267409470752, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42748; Mature: 42617

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIAQRPRVASSDGEAILNALPNPVLLVAPDGRIVDANMAAESFFEMSKQFLQREPLKQ
CCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCHHH
LIPFGSPLLALIDQVRDSGAPVNEFRVDLGTPRIGRDRQVDLHVAPLTERPAHVVVMIQE
HCCCCCHHHHHHHHHHHCCCCHHHHEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQGATPDDRMLTRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CDEADRIVTLVDRMEVFGDERPIRREPVNIHAVLDHVKRLAQSGFARNVRFTVDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
PVLGDRDQLVQIFLNLVKNAAEAVSDLGEEGEIQFTTAFRPGLRLSLPGKKTRVSLPLEF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCEEECCCEE
CVKDNGPGVAEDLLPNLFDPFVTTKQTGSGLGLALVAKIVGDHGGIVECESQPRKTNFRI
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEE
LLPMFDSGPVHRQGDDAAASGIPPRTARHPA
EEEECCCCCCCCCCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSIAQRPRVASSDGEAILNALPNPVLLVAPDGRIVDANMAAESFFEMSKQFLQREPLKQ
CCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCHHH
LIPFGSPLLALIDQVRDSGAPVNEFRVDLGTPRIGRDRQVDLHVAPLTERPAHVVVMIQE
HCCCCCHHHHHHHHHHHCCCCHHHHEEECCCCCCCCCCEEEEEEECCCCCCCEEEEEEEC
RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQLLEQGATPDDRMLTRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
CDEADRIVTLVDRMEVFGDERPIRREPVNIHAVLDHVKRLAQSGFARNVRFTVDYDPSLP
HCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
PVLGDRDQLVQIFLNLVKNAAEAVSDLGEEGEIQFTTAFRPGLRLSLPGKKTRVSLPLEF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCEEECCCEE
CVKDNGPGVAEDLLPNLFDPFVTTKQTGSGLGLALVAKIVGDHGGIVECESQPRKTNFRI
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCEE
LLPMFDSGPVHRQGDDAAASGIPPRTARHPA
EEEECCCCCCCCCCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]