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The map label for this gene is dus [H]

Identifier: 75675635

GI number: 75675635

Start: 1571189

End: 1572238

Strand: Direct

Name: dus [H]

Synonym: Nwi_1443

Alternate gene names: 75675635

Gene position: 1571189-1572238 (Clockwise)

Preceding gene: 75675632

Following gene: 75675636

Centisome position: 46.18

GC content: 65.9

Gene sequence:

>1050_bases
ATGAGCAAGAGCCTGACAGGTCCTCCTCCGTCATCAAGTCTCGGACCGTTCGCGGTCGGAGATATTTCGATCCGCAACCG
CGTTCTTCTTGCGCCGATGTCGGGGATCACGGACAGGCCGTTTCGACGTCTTACGGAGTCGCTGGGGGCGGCGCTCGTTG
TTTCGGAAATGACCGCGAGCGACGATCTCGTCCGCGGGCGTCCAATGTCCGTCCTTCGCTGTGAAGCCACCGGTCATGGG
CCGCACGTCGTTCAGCTTGCCGGCTGCGAGACGCGCTGGATGGCGGAAGCCGCGCGGATCGCCGAGGCGGCCGGCGCCGA
CATCATCGATATCAACATGGGTTGTCCCGCCCGGCACGTGACAGGCGGCCAGTCGGGTTCGGCGCTGATGCGCGATCCCG
ACCATGCCCTCGACCTGATCGAGGCGACGGTCGGCGCGGTGAACGTGCCGGTCACACTCAAAATGCGCCTCGGATGGGAC
GGCCACTCGTTCAATGCGCCTTCGCTGGCGCGGCGCGCCGAATCCGCCGGCGTGCGGATGATCACGGTTCATGGCCGGAC
GCGCTGCCAGTTCTACAAAGGCCGCGCCGATTGGCGGGCCGTGCGGGCCGTGAAGGAAGCGGTGCGCGTTCCCGTCGTCG
TCAACGGCGACATCACATCGTTCGACGCAGCCGTTGCTGCGCTGGAGGCGTCGGGGGCCGATGCGGTCATGGTGGGCCGC
GGCGCGCAGGGCCGCCCCTGGCTGCCGGGTCAGATCGGGCGGCGGCTCGAAACCGGCATCGAAGAATCCCATCCTTCGCT
CACGGATCAGTTGGCTTACATCCGCGCGCTTTATGACGACCTGCTTCTGCATTACGGCCTGCGCATCGGGCTTCGTCATG
CGCGAAAGCATCTTGGCTGGGCGCTGGATACGGCGGCAGCGCTTCGTGCCGTGCCGACACCGGTCCAGAAATCGTGGCGG
ACGAAAATTCTGACGGCCGATGATCCCTCCGGCGTACAGCGGTTACTGGTGGACGCGTTCGACGATTTCGCGTGGAGGGC
CGTGGCATGA

Upstream 100 bases:

>100_bases
GCGTAGTTGGCAAGATGCCCGGAACTGGCTATTGTGTAGGCATAAAACCATCATGCCTAATAAATAAGCTATTCGGAACG
TGGCACGGAACGATTGGACC

Downstream 100 bases:

>100_bases
CGTCGATCGCGCAGCGTCCGCGCGTCGCGTCTTCCGATGGCGAGGCCATTCTCAACGCGCTCCCTAATCCTGTGCTGCTG
GTCGCGCCCGACGGCCGGAT

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSKSLTGPPPSSSLGPFAVGDISIRNRVLLAPMSGITDRPFRRLTESLGAALVVSEMTASDDLVRGRPMSVLRCEATGHG
PHVVQLAGCETRWMAEAARIAEAAGADIIDINMGCPARHVTGGQSGSALMRDPDHALDLIEATVGAVNVPVTLKMRLGWD
GHSFNAPSLARRAESAGVRMITVHGRTRCQFYKGRADWRAVRAVKEAVRVPVVVNGDITSFDAAVAALEASGADAVMVGR
GAQGRPWLPGQIGRRLETGIEESHPSLTDQLAYIRALYDDLLLHYGLRIGLRHARKHLGWALDTAAALRAVPTPVQKSWR
TKILTADDPSGVQRLLVDAFDDFAWRAVA

Sequences:

>Translated_349_residues
MSKSLTGPPPSSSLGPFAVGDISIRNRVLLAPMSGITDRPFRRLTESLGAALVVSEMTASDDLVRGRPMSVLRCEATGHG
PHVVQLAGCETRWMAEAARIAEAAGADIIDINMGCPARHVTGGQSGSALMRDPDHALDLIEATVGAVNVPVTLKMRLGWD
GHSFNAPSLARRAESAGVRMITVHGRTRCQFYKGRADWRAVRAVKEAVRVPVVVNGDITSFDAAVAALEASGADAVMVGR
GAQGRPWLPGQIGRRLETGIEESHPSLTDQLAYIRALYDDLLLHYGLRIGLRHARKHLGWALDTAAALRAVPTPVQKSWR
TKILTADDPSGVQRLLVDAFDDFAWRAVA
>Mature_348_residues
SKSLTGPPPSSSLGPFAVGDISIRNRVLLAPMSGITDRPFRRLTESLGAALVVSEMTASDDLVRGRPMSVLRCEATGHGP
HVVQLAGCETRWMAEAARIAEAAGADIIDINMGCPARHVTGGQSGSALMRDPDHALDLIEATVGAVNVPVTLKMRLGWDG
HSFNAPSLARRAESAGVRMITVHGRTRCQFYKGRADWRAVRAVKEAVRVPVVVNGDITSFDAAVAALEASGADAVMVGRG
AQGRPWLPGQIGRRLETGIEESHPSLTDQLAYIRALYDDLLLHYGLRIGLRHARKHLGWALDTAAALRAVPTPVQKSWRT
KILTADDPSGVQRLLVDAFDDFAWRAVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=302, Percent_Identity=29.8013245033113, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI239788483, Length=273, Percent_Identity=30.03663003663, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=31.6964285714286, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=28.1632653061224, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=30.3964757709251, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789660, Length=333, Percent_Identity=42.3423423423423, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=35.042735042735, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693211, Length=299, Percent_Identity=27.4247491638796, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=283, Percent_Identity=31.095406360424, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=217, Percent_Identity=32.258064516129, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=229, Percent_Identity=31.0043668122271, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=26.9387755102041, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=259, Percent_Identity=28.1853281853282, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.8701298701299, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=223, Percent_Identity=26.0089686098655, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=31.6666666666667, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=29.4605809128631, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=30.7017543859649, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37453; Mature: 37322

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLTGPPPSSSLGPFAVGDISIRNRVLLAPMSGITDRPFRRLTESLGAALVVSEMTAS
CCCCCCCCCCCCCCCCEEEECEEECCEEEEECCCCCCCHHHHHHHHHHCCEEEEHHHCCC
DDLVRGRPMSVLRCEATGHGPHVVQLAGCETRWMAEAARIAEAAGADIIDINMGCPARHV
CCHHCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
TGGQSGSALMRDPDHALDLIEATVGAVNVPVTLKMRLGWDGHSFNAPSLARRAESAGVRM
CCCCCCCCCCCCCHHHHHHHHHHHCEEECCEEEEEEECCCCCCCCCHHHHHHHHHCCEEE
ITVHGRTRCQFYKGRADWRAVRAVKEAVRVPVVVNGDITSFDAAVAALEASGADAVMVGR
EEECCCCEEEEECCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCEEEECC
GAQGRPWLPGQIGRRLETGIEESHPSLTDQLAYIRALYDDLLLHYGLRIGLRHARKHLGW
CCCCCCCCCHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
ALDTAAALRAVPTPVQKSWRTKILTADDPSGVQRLLVDAFDDFAWRAVA
HHHHHHHHHHCCCCHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SKSLTGPPPSSSLGPFAVGDISIRNRVLLAPMSGITDRPFRRLTESLGAALVVSEMTAS
CCCCCCCCCCCCCCCEEEECEEECCEEEEECCCCCCCHHHHHHHHHHCCEEEEHHHCCC
DDLVRGRPMSVLRCEATGHGPHVVQLAGCETRWMAEAARIAEAAGADIIDINMGCPARHV
CCHHCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
TGGQSGSALMRDPDHALDLIEATVGAVNVPVTLKMRLGWDGHSFNAPSLARRAESAGVRM
CCCCCCCCCCCCCHHHHHHHHHHHCEEECCEEEEEEECCCCCCCCCHHHHHHHHHCCEEE
ITVHGRTRCQFYKGRADWRAVRAVKEAVRVPVVVNGDITSFDAAVAALEASGADAVMVGR
EEECCCCEEEEECCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCEEEECC
GAQGRPWLPGQIGRRLETGIEESHPSLTDQLAYIRALYDDLLLHYGLRIGLRHARKHLGW
CCCCCCCCCHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
ALDTAAALRAVPTPVQKSWRTKILTADDPSGVQRLLVDAFDDFAWRAVA
HHHHHHHHHHCCCCHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]