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The map label for this gene is ntrC [H]

Identifier: 75675637

GI number: 75675637

Start: 1573418

End: 1574857

Strand: Direct

Name: ntrC [H]

Synonym: Nwi_1445

Alternate gene names: 75675637

Gene position: 1573418-1574857 (Clockwise)

Preceding gene: 75675636

Following gene: 75675638

Centisome position: 46.25

GC content: 62.08

Gene sequence:

>1440_bases
ATGTCTCCAGCCAACATACTTGTCGCCGATGATGACGGAGCCATCCGCACGGTGATCAATCAGGCGCTGTCGCGTGCCGG
TTATGACGTGCGCCTGACCGGGAATGCCGCAACGCTGTGGCGATGGGTCAGCCAGGGCGAAGGAGATATCGTGATCACCG
ACGTGGTGATGCCGGACGAGAACGCCTTCGACCTTCTGCCTCGCATCAAGAAAATCAGGCCGAACCTGCCGGTCATCGTG
ATGAGCGCGCAGAACACCTTCATGACGGCGATCCGCGCCTCCGAGCGCGGCGCTTACGAATACCTTCCGAAACCGTTCGA
CCTGAAGGAACTGATCGCAATCGTCGGCCGCGCGCTCGCCGAGCCGAAGGAGAGCGCGGCCAGCCGCGGCGACGAGAATG
AAGGCGATTCCCTGCCGTTGATCGGCCGGTCCCCCGCCATGCAGGAAATCTACCGTGTTCTGGCGCGGCTGATGCAGACG
GACCTGACCGTGATGATTTCCGGCGAGTCCGGCACCGGCAAGGAACTTGTCGCGCGCGCGCTTCATGACTACGGCAAGCG
TCGCGGCGGTCCGTTCGTCGCCGTCAATATGGCGGCGATTCCCCGCGACCTGATCGAATCGGAGTTGTTCGGTCACGAAC
GCGGCGCATTCACGGGCGCGAACGCCCGCGCGTCGGGCCGTTTCGAGCAGGCCGAAGGAGGGACTCTCTTTCTCGACGAA
ATCGGCGACATGCCGATGGAGGCGCAGACACGTCTGCTTCGTGTGCTTCAGCAGGGTGAATATTGCACCGTCGGGGGAAG
GACGGCCATCAAGACGGATGTCCGCATCGTTGCGGCATCCAACAAGGATCTTCGTGTCCTGATTCAGCAGGGGCTGTTTC
GCGAGGATCTTTTTTTCCGCCTCAACGTGGTGCCGCTGCGCCTGCCGCCGCTCCGCGAACGCATCGAGGATCTGCCGGAT
CTGATCAGGCATTTTTTCGTGCTTGCCGAAAAGGATGGACTGCCGCCCAAGAAGCTGGACGCGCTTGCGCTAGAACGCTT
GAAGCGGCACCGCTGGCCCGGCAACGTCCGGGAGCTTGAAAACCTTGCGCGCAGGCTGGCCGCGCTCTACCCGCAGGATG
TCATCACCGCTCCGGTGATCGATTGCGAGCTTGCATCTCCCGAGGCGACCTCCGGAAGTAACGCCCAGAACATCAATAGT
CTCGGTGGCGCCGTCGAGATGTACATGTCGTCGCTTTTCTCAGGCTCCCCGAACGGGCTCCCGCCGCCTGGCCTGTATCA
ACGCGTTCTCAGGGAGATTGAGGTCCCGCTTCTCACCGCCGTGCTGGCGGCGACGCGCGGCAACCAGATTCGCGCCGCCG
ACCTTTTGGGTTTGAACCGCAACACGCTCCGTAAAAAAATCCGCGATCTGGATATCCAGGTCTACCGTTCCGGAGGATAG

Upstream 100 bases:

>100_bases
CTGCCGATGTTCGATTCCGGTCCGGTACACCGGCAGGGCGATGACGCTGCCGCGTCCGGAATACCGCCACGCACCGCGCG
TCATCCAGCATGAGGACAAA

Downstream 100 bases:

>100_bases
GTAGCCGAGTCTACCGGCAGTTTTTCCGCCATTCACGATCGAACCGGACTCAATCGATTGTTGCCAGTCGCGGCTGATGT
TCGCGCCGCGGAAATGTCCC

Product: helix-turn-helix, Fis-type / nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSPANILVADDDGAIRTVINQALSRAGYDVRLTGNAATLWRWVSQGEGDIVITDVVMPDENAFDLLPRIKKIRPNLPVIV
MSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALAEPKESAASRGDENEGDSLPLIGRSPAMQEIYRVLARLMQT
DLTVMISGESGTGKELVARALHDYGKRRGGPFVAVNMAAIPRDLIESELFGHERGAFTGANARASGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYCTVGGRTAIKTDVRIVAASNKDLRVLIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPD
LIRHFFVLAEKDGLPPKKLDALALERLKRHRWPGNVRELENLARRLAALYPQDVITAPVIDCELASPEATSGSNAQNINS
LGGAVEMYMSSLFSGSPNGLPPPGLYQRVLREIEVPLLTAVLAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG

Sequences:

>Translated_479_residues
MSPANILVADDDGAIRTVINQALSRAGYDVRLTGNAATLWRWVSQGEGDIVITDVVMPDENAFDLLPRIKKIRPNLPVIV
MSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALAEPKESAASRGDENEGDSLPLIGRSPAMQEIYRVLARLMQT
DLTVMISGESGTGKELVARALHDYGKRRGGPFVAVNMAAIPRDLIESELFGHERGAFTGANARASGRFEQAEGGTLFLDE
IGDMPMEAQTRLLRVLQQGEYCTVGGRTAIKTDVRIVAASNKDLRVLIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPD
LIRHFFVLAEKDGLPPKKLDALALERLKRHRWPGNVRELENLARRLAALYPQDVITAPVIDCELASPEATSGSNAQNINS
LGGAVEMYMSSLFSGSPNGLPPPGLYQRVLREIEVPLLTAVLAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG
>Mature_478_residues
SPANILVADDDGAIRTVINQALSRAGYDVRLTGNAATLWRWVSQGEGDIVITDVVMPDENAFDLLPRIKKIRPNLPVIVM
SAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALAEPKESAASRGDENEGDSLPLIGRSPAMQEIYRVLARLMQTD
LTVMISGESGTGKELVARALHDYGKRRGGPFVAVNMAAIPRDLIESELFGHERGAFTGANARASGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQGEYCTVGGRTAIKTDVRIVAASNKDLRVLIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPDL
IRHFFVLAEKDGLPPKKLDALALERLKRHRWPGNVRELENLARRLAALYPQDVITAPVIDCELASPEATSGSNAQNINSL
GGAVEMYMSSLFSGSPNGLPPPGLYQRVLREIEVPLLTAVLAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=45.3191489361702, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1788550, Length=476, Percent_Identity=36.5546218487395, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1790437, Length=464, Percent_Identity=38.1465517241379, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=35.7758620689655, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=43.4131736526946, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789233, Length=342, Percent_Identity=39.766081871345, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI87082117, Length=266, Percent_Identity=47.3684210526316, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=244, Percent_Identity=46.7213114754098, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=44.578313253012, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081872, Length=342, Percent_Identity=38.0116959064327, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1787583, Length=243, Percent_Identity=41.1522633744856, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1789828, Length=257, Percent_Identity=33.4630350194552, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081858, Length=204, Percent_Identity=35.7843137254902, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=30.2752293577982, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786784, Length=109, Percent_Identity=27.5229357798165, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790552, Length=137, Percent_Identity=30.6569343065693, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52719; Mature: 52588

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPANILVADDDGAIRTVINQALSRAGYDVRLTGNAATLWRWVSQGEGDIVITDVVMPDE
CCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKIRPNLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALA
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKESAASRGDENEGDSLPLIGRSPAMQEIYRVLARLMQTDLTVMISGESGTGKELVARA
CCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHDYGKRRGGPFVAVNMAAIPRDLIESELFGHERGAFTGANARASGRFEQAEGGTLFLDE
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYCTVGGRTAIKTDVRIVAASNKDLRVLIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEEECCCEEEEECEEEEEECCCCHHHHHHHCCHHHCCEEE
LNVVPLRLPPLRERIEDLPDLIRHFFVLAEKDGLPPKKLDALALERLKRHRWPGNVRELE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NLARRLAALYPQDVITAPVIDCELASPEATSGSNAQNINSLGGAVEMYMSSLFSGSPNGL
HHHHHHHHHCCHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PPPGLYQRVLREIEVPLLTAVLAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
SPANILVADDDGAIRTVINQALSRAGYDVRLTGNAATLWRWVSQGEGDIVITDVVMPDE
CCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECCCC
NAFDLLPRIKKIRPNLPVIVMSAQNTFMTAIRASERGAYEYLPKPFDLKELIAIVGRALA
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHC
EPKESAASRGDENEGDSLPLIGRSPAMQEIYRVLARLMQTDLTVMISGESGTGKELVARA
CCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHH
LHDYGKRRGGPFVAVNMAAIPRDLIESELFGHERGAFTGANARASGRFEQAEGGTLFLDE
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
IGDMPMEAQTRLLRVLQQGEYCTVGGRTAIKTDVRIVAASNKDLRVLIQQGLFREDLFFR
HCCCCHHHHHHHHHHHHCCCEEEECCCEEEEECEEEEEECCCCHHHHHHHCCHHHCCEEE
LNVVPLRLPPLRERIEDLPDLIRHFFVLAEKDGLPPKKLDALALERLKRHRWPGNVRELE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
NLARRLAALYPQDVITAPVIDCELASPEATSGSNAQNINSLGGAVEMYMSSLFSGSPNGL
HHHHHHHHHCCHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PPPGLYQRVLREIEVPLLTAVLAATRGNQIRAADLLGLNRNTLRKKIRDLDIQVYRSGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]