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The map label for this gene is murF [H]

Identifier: 75675240

GI number: 75675240

Start: 1140913

End: 1142343

Strand: Direct

Name: murF [H]

Synonym: Nwi_1047

Alternate gene names: 75675240

Gene position: 1140913-1142343 (Clockwise)

Preceding gene: 75675239

Following gene: 75675241

Centisome position: 33.54

GC content: 66.6

Gene sequence:

>1431_bases
ATGAGCGCGCGCCCGTTGTGGACGTTGGACGAAATGGCCGCGGCGATGCGCGCGGAAGGGCGAGGGACCTTGCCCGCAGC
GATCACCGGCATTTCCATCGACAGCCGCACCGTTGCGCCGGGCGAGGCTTATTTCGCGATCAAGGGCGATGTGCATGACG
GCCACGCTTTCGTTGGTGCCGCCTTGAAGGCGGGCGCGGCGCTGGCGGTGGTCGAGGCCGCGCGGCGCGATCAGTTCGCC
CTTGAGTTGCCGTTGCTGGTCGTCGATGACGTGCTTGCAGCCTTGCGCGATCTGGCGCGGGCGTCGCGCGCGCGCCTCGC
CGCTCAGGTGATCGCGGTGACGGGCTCCGTCGGCAAGACTTCCACAAAGGAGGCGCTGCGCGGTGTCCTAGGCGCGCAGG
GCAAGACCCACGTCTCGGTGGCTTCGTTCAACAACCACTGGGGCGTGCCGCTGTCGCTGTCGCGCTGTCCGGCGGACGCG
CGCTTCGCGGTCTTCGAGATCGGGATGAATCATGCCGGCGAGATCACGCCGCTGGTGAAGATGGTGCGGCCGCATGTGGC
GATCATCACCACGGTCGAGCCCGTGCATCTCGAATTCTTCTCCGGCGTCGAGGCGATCGCCGACGCCAAGGCGGAAATTT
TCGAAGGCGTGGAGCCGGACGGCGCCGTCGTGCTCAACCGTGATAACGCGCAGTTCGCGCGGTTGCAGGAATGCGCCAGG
AAACTCGGCATCGCGAAGATCGTCACCTTCGGCGCGGACGAGAAGGCCGATGCGCGCCTGCTCGATCTGGCGTTGCATCC
GGCTGGCTCGGCCGTGCACGCTGAAATCCTCGGCAGTGAGTTGACCTACAAGCTCGGCATGCCCGGCCGTCACATGGCGA
TGAATTCGCTCGCGGTGCTGGCGGCGGCGTCGCTGGCCGGCGCTGATCTTGCGCTTGCGGGTCTTGCGCTGTCGCAGGCG
CAGCCGGCCGCGGGACGCGGCGTGCGTCGGGCGCTGGCCGTGAGGGGCGGCGAGGCGCTCCTGATCGATGAGAGCTATAA
CGCCAATCCTGCTTCGATGGCGGCGGCGCTGAACGTGCTCGGCGAGGCCAAAGTCGGGACGCAGGGTCGGCGCATCGCGG
TGCTGGGCGACATGCTGGAGCTTGGTCCCAGAAGCGCGGACCTGCATCGCGACCTGACCGGGGCGATCGATGCCAATGAC
GTCGACATGATATTTTGCTGCGGTCCCCTGATGCGCAATCTGTGGAATGCGCTTTCCTCCGGTAGGCGGGGCGGTTATGC
AGAGGACGCCGCCGCGCTCGAATCGCAGGTGACGGCGGCGATCCGTTCCGGTGACGTCATCATGGTCAAGGGCTCGCTGG
GATCGCGGATGAAAGTGATCGTGACCGCGCTCGAGAGGCGATATTCCGGCAGCGCGGCTGAAATTCTGTAA

Upstream 100 bases:

>100_bases
TCGCGGGCAAGGGTCACGAGACCGGACAGATCGTCGGCGATCGCGTGCTGCCGTTCAGCGATCACGAGGCCGCCAGCGCG
GCGCTGACGTCGAGGCGGAT

Downstream 100 bases:

>100_bases
GTTGCGGGGTGCCCCTTTGAATCCGGAGTTCGCTCAAGGGAGGCTTGCCAGAACTGGCGAACTTCGGAGTCGGGATACCC
GATGTTTTACTGGTTGATCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSARPLWTLDEMAAAMRAEGRGTLPAAITGISIDSRTVAPGEAYFAIKGDVHDGHAFVGAALKAGAALAVVEAARRDQFA
LELPLLVVDDVLAALRDLARASRARLAAQVIAVTGSVGKTSTKEALRGVLGAQGKTHVSVASFNNHWGVPLSLSRCPADA
RFAVFEIGMNHAGEITPLVKMVRPHVAIITTVEPVHLEFFSGVEAIADAKAEIFEGVEPDGAVVLNRDNAQFARLQECAR
KLGIAKIVTFGADEKADARLLDLALHPAGSAVHAEILGSELTYKLGMPGRHMAMNSLAVLAAASLAGADLALAGLALSQA
QPAAGRGVRRALAVRGGEALLIDESYNANPASMAAALNVLGEAKVGTQGRRIAVLGDMLELGPRSADLHRDLTGAIDAND
VDMIFCCGPLMRNLWNALSSGRRGGYAEDAAALESQVTAAIRSGDVIMVKGSLGSRMKVIVTALERRYSGSAAEIL

Sequences:

>Translated_476_residues
MSARPLWTLDEMAAAMRAEGRGTLPAAITGISIDSRTVAPGEAYFAIKGDVHDGHAFVGAALKAGAALAVVEAARRDQFA
LELPLLVVDDVLAALRDLARASRARLAAQVIAVTGSVGKTSTKEALRGVLGAQGKTHVSVASFNNHWGVPLSLSRCPADA
RFAVFEIGMNHAGEITPLVKMVRPHVAIITTVEPVHLEFFSGVEAIADAKAEIFEGVEPDGAVVLNRDNAQFARLQECAR
KLGIAKIVTFGADEKADARLLDLALHPAGSAVHAEILGSELTYKLGMPGRHMAMNSLAVLAAASLAGADLALAGLALSQA
QPAAGRGVRRALAVRGGEALLIDESYNANPASMAAALNVLGEAKVGTQGRRIAVLGDMLELGPRSADLHRDLTGAIDAND
VDMIFCCGPLMRNLWNALSSGRRGGYAEDAAALESQVTAAIRSGDVIMVKGSLGSRMKVIVTALERRYSGSAAEIL
>Mature_475_residues
SARPLWTLDEMAAAMRAEGRGTLPAAITGISIDSRTVAPGEAYFAIKGDVHDGHAFVGAALKAGAALAVVEAARRDQFAL
ELPLLVVDDVLAALRDLARASRARLAAQVIAVTGSVGKTSTKEALRGVLGAQGKTHVSVASFNNHWGVPLSLSRCPADAR
FAVFEIGMNHAGEITPLVKMVRPHVAIITTVEPVHLEFFSGVEAIADAKAEIFEGVEPDGAVVLNRDNAQFARLQECARK
LGIAKIVTFGADEKADARLLDLALHPAGSAVHAEILGSELTYKLGMPGRHMAMNSLAVLAAASLAGADLALAGLALSQAQ
PAAGRGVRRALAVRGGEALLIDESYNANPASMAAALNVLGEAKVGTQGRRIAVLGDMLELGPRSADLHRDLTGAIDANDV
DMIFCCGPLMRNLWNALSSGRRGGYAEDAAALESQVTAAIRSGDVIMVKGSLGSRMKVIVTALERRYSGSAAEIL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=466, Percent_Identity=32.618025751073, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49578; Mature: 49446

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARPLWTLDEMAAAMRAEGRGTLPAAITGISIDSRTVAPGEAYFAIKGDVHDGHAFVGA
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHH
ALKAGAALAVVEAARRDQFALELPLLVVDDVLAALRDLARASRARLAAQVIAVTGSVGKT
HHHCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
STKEALRGVLGAQGKTHVSVASFNNHWGVPLSLSRCPADARFAVFEIGMNHAGEITPLVK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCCCCCEEEEEECCCCCCCHHHHHH
MVRPHVAIITTVEPVHLEFFSGVEAIADAKAEIFEGVEPDGAVVLNRDNAQFARLQECAR
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
KLGIAKIVTFGADEKADARLLDLALHPAGSAVHAEILGSELTYKLGMPGRHMAMNSLAVL
HCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
AAASLAGADLALAGLALSQAQPAAGRGVRRALAVRGGEALLIDESYNANPASMAAALNVL
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
GEAKVGTQGRRIAVLGDMLELGPRSADLHRDLTGAIDANDVDMIFCCGPLMRNLWNALSS
HCCCCCCCCCEEEEEHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHC
GRRGGYAEDAAALESQVTAAIRSGDVIMVKGSLGSRMKVIVTALERRYSGSAAEIL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SARPLWTLDEMAAAMRAEGRGTLPAAITGISIDSRTVAPGEAYFAIKGDVHDGHAFVGA
CCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHH
ALKAGAALAVVEAARRDQFALELPLLVVDDVLAALRDLARASRARLAAQVIAVTGSVGKT
HHHCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
STKEALRGVLGAQGKTHVSVASFNNHWGVPLSLSRCPADARFAVFEIGMNHAGEITPLVK
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCEECCCCCCCCCEEEEEECCCCCCCHHHHHH
MVRPHVAIITTVEPVHLEFFSGVEAIADAKAEIFEGVEPDGAVVLNRDNAQFARLQECAR
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHH
KLGIAKIVTFGADEKADARLLDLALHPAGSAVHAEILGSELTYKLGMPGRHMAMNSLAVL
HCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
AAASLAGADLALAGLALSQAQPAAGRGVRRALAVRGGEALLIDESYNANPASMAAALNVL
HHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
GEAKVGTQGRRIAVLGDMLELGPRSADLHRDLTGAIDANDVDMIFCCGPLMRNLWNALSS
HCCCCCCCCCEEEEEHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHC
GRRGGYAEDAAALESQVTAAIRSGDVIMVKGSLGSRMKVIVTALERRYSGSAAEIL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893; 9274032 [H]