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The map label for this gene is murE [H]

Identifier: 75675239

GI number: 75675239

Start: 1139459

End: 1140916

Strand: Direct

Name: murE [H]

Synonym: Nwi_1046

Alternate gene names: 75675239

Gene position: 1139459-1140916 (Clockwise)

Preceding gene: 75675238

Following gene: 75675240

Centisome position: 33.49

GC content: 67.22

Gene sequence:

>1458_bases
ATGAAACTTCGCGAGCTTTTCGGCGACGACGCCACCATGGACGCGCCGGCCGGCGAGCTTGCCGTCACGGGTCTCGCGGC
GGATAGCCGCGCGGTGAAGCCCGGAGATCTCTTTTTCGCGCTTGCCGGCTCGAAAACGGATGGCGCGCGGTTCATCGACG
CCGCGATCGCGTCGGGCGCTGTCGCGGTCGCCGGTGATCATGCGCCTGCGCGCGGGCGCGTCCCGTTCATCGCAACGCCG
AATCCGCGGCGCGCGCTGGCGCTGGCGGCGGCAAAATTCTATCCGCGACAGCCCGCAACCATTGCCGCCGTGACCGGGAC
CAGCGGCAAAACCTCGGTCGCGGCCTTCACCCGGCAGATCTGGCAGCGGCTCGGATATTTGTCCGCGAGCATCGGCACCG
TCGGCGTGGTCGCGCCCTCGCGCGCGGTCTACGGCTCGGTGACGACGCCCGATCCGATCGAGTTGCATCGGGAGCTGGAT
CAGCTCGCGGGCGAAGGCGTCACGCATCTTGCGCTGGAAGCATCCTCGCACGGTCTTGATCAATATCGGGTCGATGGCGT
GCGGGTGCGCGCCGCCGGCTTCACCAACCTGTCGCGCGATCATATGGATTATCATCCGACGGTCGCGCATTATCTCGAGA
CCAAGCTGCGGCTGTTCCGCGATCTGGTCGAATCGGACGGCATCGCGGTGATCTGCGCCGATCATGACTGCTCCGATACC
GTGATCGAAGCGGCGGGCGCGCGCAACGTGCCGGTGATGCGTGTCGGCCGCCGTGGCGACGGCGCGCGGATGGGCATTCG
CCTGGTCGAGGCGGCGATCGATGGTTTTGCGCAACGGGTCACGCTCGAACACGCAGGGCGCGAGCGTGTCATCCGCCTGC
CTCTGGTCGGAGAATTCCAGATCGAGAATGCGCTGGTGTCGGCCGGTCTCGTCATCGGCACCGGTGGCGACGTCGATCAG
GCGTTCGCGGCGCTCGAGCATCTCGAAGGCGCCAGGGGGCGGATCGAACGGATCGGTGAGCGCAACGGCGCTCAGGTCTT
TGTTGACTACGCTCACAAACCGGACGCGCTGGCGAAGGCGTTGCAGGCGTTGAGGCCCTATGCCAAGCGCAAGCTCGTGG
TGGTGTTCGGAGCAGGCGGTGATCGCGATGCAGGCAAGCGCCCGCTGATGGGAGCGATCGCCGCTGATAACGCGGATGTG
GTGATCGTGACCGACGATAATCCGCGCAGTGAAGATCCCGCCGCGATCCGCGCCGCGATCCTGGCCGCCGCTCCGGGTGC
CCGCGAGATCGGCGATCGCGCCGAGGCGATCGGGACCGCGATCGCGGGATTGCAGTCGGGAGACGCCTTGCTGATCGCGG
GCAAGGGTCACGAGACCGGACAGATCGTCGGCGATCGCGTGCTGCCGTTCAGCGATCACGAGGCCGCCAGCGCGGCGCTG
ACGTCGAGGCGGATATGA

Upstream 100 bases:

>100_bases
ATCCAGGGCGACGCAGTAGGGCGAGGTTTGTTTCATTTCAGCGCGCCTCGCGGCAAACGTCGGGCTGCGGCGTCAAATTG
AAGCTACGGCTGGGGAGATC

Downstream 100 bases:

>100_bases
GCGCGCGCCCGTTGTGGACGTTGGACGAAATGGCCGCGGCGATGCGCGCGGAAGGGCGAGGGACCTTGCCCGCAGCGATC
ACCGGCATTTCCATCGACAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKLRELFGDDATMDAPAGELAVTGLAADSRAVKPGDLFFALAGSKTDGARFIDAAIASGAVAVAGDHAPARGRVPFIATP
NPRRALALAAAKFYPRQPATIAAVTGTSGKTSVAAFTRQIWQRLGYLSASIGTVGVVAPSRAVYGSVTTPDPIELHRELD
QLAGEGVTHLALEASSHGLDQYRVDGVRVRAAGFTNLSRDHMDYHPTVAHYLETKLRLFRDLVESDGIAVICADHDCSDT
VIEAAGARNVPVMRVGRRGDGARMGIRLVEAAIDGFAQRVTLEHAGRERVIRLPLVGEFQIENALVSAGLVIGTGGDVDQ
AFAALEHLEGARGRIERIGERNGAQVFVDYAHKPDALAKALQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAADNADV
VIVTDDNPRSEDPAAIRAAILAAAPGAREIGDRAEAIGTAIAGLQSGDALLIAGKGHETGQIVGDRVLPFSDHEAASAAL
TSRRI

Sequences:

>Translated_485_residues
MKLRELFGDDATMDAPAGELAVTGLAADSRAVKPGDLFFALAGSKTDGARFIDAAIASGAVAVAGDHAPARGRVPFIATP
NPRRALALAAAKFYPRQPATIAAVTGTSGKTSVAAFTRQIWQRLGYLSASIGTVGVVAPSRAVYGSVTTPDPIELHRELD
QLAGEGVTHLALEASSHGLDQYRVDGVRVRAAGFTNLSRDHMDYHPTVAHYLETKLRLFRDLVESDGIAVICADHDCSDT
VIEAAGARNVPVMRVGRRGDGARMGIRLVEAAIDGFAQRVTLEHAGRERVIRLPLVGEFQIENALVSAGLVIGTGGDVDQ
AFAALEHLEGARGRIERIGERNGAQVFVDYAHKPDALAKALQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAADNADV
VIVTDDNPRSEDPAAIRAAILAAAPGAREIGDRAEAIGTAIAGLQSGDALLIAGKGHETGQIVGDRVLPFSDHEAASAAL
TSRRI
>Mature_485_residues
MKLRELFGDDATMDAPAGELAVTGLAADSRAVKPGDLFFALAGSKTDGARFIDAAIASGAVAVAGDHAPARGRVPFIATP
NPRRALALAAAKFYPRQPATIAAVTGTSGKTSVAAFTRQIWQRLGYLSASIGTVGVVAPSRAVYGSVTTPDPIELHRELD
QLAGEGVTHLALEASSHGLDQYRVDGVRVRAAGFTNLSRDHMDYHPTVAHYLETKLRLFRDLVESDGIAVICADHDCSDT
VIEAAGARNVPVMRVGRRGDGARMGIRLVEAAIDGFAQRVTLEHAGRERVIRLPLVGEFQIENALVSAGLVIGTGGDVDQ
AFAALEHLEGARGRIERIGERNGAQVFVDYAHKPDALAKALQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAADNADV
VIVTDDNPRSEDPAAIRAAILAAAPGAREIGDRAEAIGTAIAGLQSGDALLIAGKGHETGQIVGDRVLPFSDHEAASAAL
TSRRI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=488, Percent_Identity=37.5, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1786274, Length=377, Percent_Identity=27.8514588859416, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50802; Mature: 50802

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRELFGDDATMDAPAGELAVTGLAADSRAVKPGDLFFALAGSKTDGARFIDAAIASGA
CCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCE
VAVAGDHAPARGRVPFIATPNPRRALALAAAKFYPRQPATIAAVTGTSGKTSVAAFTRQI
EEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
WQRLGYLSASIGTVGVVAPSRAVYGSVTTPDPIELHRELDQLAGEGVTHLALEASSHGLD
HHHHCHHECCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
QYRVDGVRVRAAGFTNLSRDHMDYHPTVAHYLETKLRLFRDLVESDGIAVICADHDCSDT
CEEECCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHH
VIEAAGARNVPVMRVGRRGDGARMGIRLVEAAIDGFAQRVTLEHAGRERVIRLPLVGEFQ
HHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
IENALVSAGLVIGTGGDVDQAFAALEHLEGARGRIERIGERNGAQVFVDYAHKPDALAKA
HHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
LQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAADNADVVIVTDDNPRSEDPAAIRAAI
HHHHHHHHHCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
LAAAPGAREIGDRAEAIGTAIAGLQSGDALLIAGKGHETGQIVGDRVLPFSDHEAASAAL
HHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHHHHH
TSRRI
HHCCC
>Mature Secondary Structure
MKLRELFGDDATMDAPAGELAVTGLAADSRAVKPGDLFFALAGSKTDGARFIDAAIASGA
CCCHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCE
VAVAGDHAPARGRVPFIATPNPRRALALAAAKFYPRQPATIAAVTGTSGKTSVAAFTRQI
EEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
WQRLGYLSASIGTVGVVAPSRAVYGSVTTPDPIELHRELDQLAGEGVTHLALEASSHGLD
HHHHCHHECCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
QYRVDGVRVRAAGFTNLSRDHMDYHPTVAHYLETKLRLFRDLVESDGIAVICADHDCSDT
CEEECCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHH
VIEAAGARNVPVMRVGRRGDGARMGIRLVEAAIDGFAQRVTLEHAGRERVIRLPLVGEFQ
HHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
IENALVSAGLVIGTGGDVDQAFAALEHLEGARGRIERIGERNGAQVFVDYAHKPDALAKA
HHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHH
LQALRPYAKRKLVVVFGAGGDRDAGKRPLMGAIAADNADVVIVTDDNPRSEDPAAIRAAI
HHHHHHHHHCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
LAAAPGAREIGDRAEAIGTAIAGLQSGDALLIAGKGHETGQIVGDRVLPFSDHEAASAAL
HHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHHHHHH
TSRRI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]