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The map label for this gene is penA [H]

Identifier: 75675238

GI number: 75675238

Start: 1137638

End: 1139377

Strand: Direct

Name: penA [H]

Synonym: Nwi_1045

Alternate gene names: 75675238

Gene position: 1137638-1139377 (Clockwise)

Preceding gene: 75675237

Following gene: 75675239

Centisome position: 33.44

GC content: 63.45

Gene sequence:

>1740_bases
ATGATCGGCCAGAACAGGCTTCCGCCGGCGCTCTGGAGTCAGCGCCTGATCCGCAATCTGCTCTACGGCCGCAATGTGGA
TCGCGCCGCCAAGGCGCGGGTGCGCGTCGGTCTCGTCATCATCTTGTTCGCGCTGATCTATACGGTGGTCGCGGCGCGTC
TTGTCATGTTCGCGGCCTTTCCGGATGATCGTGGAGGGCGCCGGACCGCCTCGCAGGACGCGATCGCCACGGCGCGGCCC
GATATCATCGATCGCAACGGCCAGGTTCTTGCGACCGATATCAAGTCGCCGAGCCTGTTCGGCGAGCCACGCCGCCTCAT
CGACAGGGACGAGGCGGTCGAGTTGCTGACGGCGACGCTACCGGATCTGGAGACCTCCGAGGTTCGACAGCGTCTTGGCA
CTCGCAAGGGTTTTGTCTGGCTGAAACGCGAGATCACCCCTGCGCAGCAAAAGGAAATTCACAGTCTCGGCATTCCCGGC
ATCGGCTTCCTGCGCGAGAACAAGCGCGTTTATCCAAGCGGGCGCGAGGTCGCGCACCTGATCGGGCTCGTCAACATCGA
TAACCAGGGCATCGCCGGGATGGAGAAGTGGCTCGACAGCAACGGGCTCGCTGACCTGCACCGCGCCGGCTTCGCCACCG
ACCGCCTGCAGAAGCCGGTGGAGTTGTCGATCGACCTGCGTGTCGAGCACGCGCTGCGCGACGAGCTTCTGAAAGCCAAG
GATCATTATCGGGCCAAGGCCGCCTCGGGCATCGTCTCCAACGTGCGGACCGGCGAGATCGTCGCGATGGTCTCCTTGCC
GGATTTCGATCCCAACAATCCGAAGGAGGCGCATGATCCCGATCGCATCAACCGCCTGACCACGGGCGTGTACGAGATGG
GATCGACCTTCAAGGCGTTCACGCTAGCGATGGCGCTCGATTCCGGCAGGTATGGCCTGAATTCGATGTGGGATGCGCGT
GCTCCGCTGCGCTACGGCCGCTTCACGATTCACGACGACCACTCGATGGGTCGCTTCATCAACATGAAGGAAGTGTTCAC
CTACTCATCCAACATCGGCGCGGCGCGGATCGCGCTTGCGCAAGGCGTCGAGGCGCACAAGGCGTTCCTGCGCAGGATGG
GCCAGATGGATCGCCTGCGCACGGAGTTGCCGGAAAGCGCCTCGCCGATCCTGCCGCGCAAGTGGGGTGAACTGAACACC
GTCACCATCGCGTTCGGGCACGGCATGGCGGTGGCGCCGTTGCAGGCGGTGATGGGCATCGATGCGCTGGTCAACGGCGG
CTGGCTGATTCCGCCCACCTTCCTGAAGCGCACCGAAGAGGAAGCGATGGCGGTCGCCAGGCGCGTCATCAAGCAGGAGA
CCAGCGACAAGATGCGCTTTCTGCTGCGACTGAACGCCGAAGTGGGGACCGCGAGGAAGGCCGATGTCAAAGGCTACTAT
GTCGGCGGCAAGACCGGCACTTCTGAGAAGGTCATCAACGGCCGCTACGCGAAAAAACGCGTGCTCAATTCTTTCACCGC
GATTCTGCCGGCGGACAATCCCCGATACCAGATTCTGGTCATGCTGGACGAGCCGCAGCCGCTCAAGGAAACCCATGGCT
TCATCACCTCCGGCTGGAATGCGGTTCCGACCGGCGGCAAGGTTATCGCGCGCATCGCCCCGCTGCTCGGTGTGCAGCCC
CGGTTCGATCTGCCGCCGTCCGAGCGCCTCATTCTTGCGGCATCCAGGGCGACGCAGTAG

Upstream 100 bases:

>100_bases
GCCTGGTGCCGCCCGGCACCTCCGATCCGATCGGCGCCATGATCGAGATTATCGATCCCGACGTCATCACCGGCTCGCTG
CCGTCGCAGGAGGCCCCGCA

Downstream 100 bases:

>100_bases
GGCGAGGTTTGTTTCATTTCAGCGCGCCTCGCGGCAAACGTCGGGCTGCGGCGTCAAATTGAAGCTACGGCTGGGGAGAT
CATGAAACTTCGCGAGCTTT

Product: penicillin-binding protein, transpeptidase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MIGQNRLPPALWSQRLIRNLLYGRNVDRAAKARVRVGLVIILFALIYTVVAARLVMFAAFPDDRGGRRTASQDAIATARP
DIIDRNGQVLATDIKSPSLFGEPRRLIDRDEAVELLTATLPDLETSEVRQRLGTRKGFVWLKREITPAQQKEIHSLGIPG
IGFLRENKRVYPSGREVAHLIGLVNIDNQGIAGMEKWLDSNGLADLHRAGFATDRLQKPVELSIDLRVEHALRDELLKAK
DHYRAKAASGIVSNVRTGEIVAMVSLPDFDPNNPKEAHDPDRINRLTTGVYEMGSTFKAFTLAMALDSGRYGLNSMWDAR
APLRYGRFTIHDDHSMGRFINMKEVFTYSSNIGAARIALAQGVEAHKAFLRRMGQMDRLRTELPESASPILPRKWGELNT
VTIAFGHGMAVAPLQAVMGIDALVNGGWLIPPTFLKRTEEEAMAVARRVIKQETSDKMRFLLRLNAEVGTARKADVKGYY
VGGKTGTSEKVINGRYAKKRVLNSFTAILPADNPRYQILVMLDEPQPLKETHGFITSGWNAVPTGGKVIARIAPLLGVQP
RFDLPPSERLILAASRATQ

Sequences:

>Translated_579_residues
MIGQNRLPPALWSQRLIRNLLYGRNVDRAAKARVRVGLVIILFALIYTVVAARLVMFAAFPDDRGGRRTASQDAIATARP
DIIDRNGQVLATDIKSPSLFGEPRRLIDRDEAVELLTATLPDLETSEVRQRLGTRKGFVWLKREITPAQQKEIHSLGIPG
IGFLRENKRVYPSGREVAHLIGLVNIDNQGIAGMEKWLDSNGLADLHRAGFATDRLQKPVELSIDLRVEHALRDELLKAK
DHYRAKAASGIVSNVRTGEIVAMVSLPDFDPNNPKEAHDPDRINRLTTGVYEMGSTFKAFTLAMALDSGRYGLNSMWDAR
APLRYGRFTIHDDHSMGRFINMKEVFTYSSNIGAARIALAQGVEAHKAFLRRMGQMDRLRTELPESASPILPRKWGELNT
VTIAFGHGMAVAPLQAVMGIDALVNGGWLIPPTFLKRTEEEAMAVARRVIKQETSDKMRFLLRLNAEVGTARKADVKGYY
VGGKTGTSEKVINGRYAKKRVLNSFTAILPADNPRYQILVMLDEPQPLKETHGFITSGWNAVPTGGKVIARIAPLLGVQP
RFDLPPSERLILAASRATQ
>Mature_579_residues
MIGQNRLPPALWSQRLIRNLLYGRNVDRAAKARVRVGLVIILFALIYTVVAARLVMFAAFPDDRGGRRTASQDAIATARP
DIIDRNGQVLATDIKSPSLFGEPRRLIDRDEAVELLTATLPDLETSEVRQRLGTRKGFVWLKREITPAQQKEIHSLGIPG
IGFLRENKRVYPSGREVAHLIGLVNIDNQGIAGMEKWLDSNGLADLHRAGFATDRLQKPVELSIDLRVEHALRDELLKAK
DHYRAKAASGIVSNVRTGEIVAMVSLPDFDPNNPKEAHDPDRINRLTTGVYEMGSTFKAFTLAMALDSGRYGLNSMWDAR
APLRYGRFTIHDDHSMGRFINMKEVFTYSSNIGAARIALAQGVEAHKAFLRRMGQMDRLRTELPESASPILPRKWGELNT
VTIAFGHGMAVAPLQAVMGIDALVNGGWLIPPTFLKRTEEEAMAVARRVIKQETSDKMRFLLRLNAEVGTARKADVKGYY
VGGKTGTSEKVINGRYAKKRVLNSFTAILPADNPRYQILVMLDEPQPLKETHGFITSGWNAVPTGGKVIARIAPLLGVQP
RFDLPPSERLILAASRATQ

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=559, Percent_Identity=28.2647584973166, Blast_Score=184, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 64240; Mature: 64240

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS00018 EF_HAND_1 ; PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGQNRLPPALWSQRLIRNLLYGRNVDRAAKARVRVGLVIILFALIYTVVAARLVMFAAF
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDDRGGRRTASQDAIATARPDIIDRNGQVLATDIKSPSLFGEPRRLIDRDEAVELLTATL
CCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCHHHHHHHHHHHC
PDLETSEVRQRLGTRKGFVWLKREITPAQQKEIHSLGIPGIGFLRENKRVYPSGREVAHL
CCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCEECCCCHHHHHH
IGLVNIDNQGIAGMEKWLDSNGLADLHRAGFATDRLQKPVELSIDLRVEHALRDELLKAK
HEEEEECCCCCHHHHHHHCCCCCHHHHHCCCCHHHHCCCCEEEEEEEHHHHHHHHHHHHH
DHYRAKAASGIVSNVRTGEIVAMVSLPDFDPNNPKEAHDPDRINRLTTGVYEMGSTFKAF
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
TLAMALDSGRYGLNSMWDARAPLRYGRFTIHDDHSMGRFINMKEVFTYSSNIGAARIALA
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECHHHHHEECCCCCHHHHHHH
QGVEAHKAFLRRMGQMDRLRTELPESASPILPRKWGELNTVTIAFGHGMAVAPLQAVMGI
HCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
DALVNGGWLIPPTFLKRTEEEAMAVARRVIKQETSDKMRFLLRLNAEVGTARKADVKGYY
HHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHEECCCCCCCCCCCCCEEE
VGGKTGTSEKVINGRYAKKRVLNSFTAILPADNPRYQILVMLDEPQPLKETHGFITSGWN
ECCCCCCCCHHHCCHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCHHHHCCCEECCCC
AVPTGGKVIARIAPLLGVQPRFDLPPSERLILAASRATQ
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MIGQNRLPPALWSQRLIRNLLYGRNVDRAAKARVRVGLVIILFALIYTVVAARLVMFAAF
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDDRGGRRTASQDAIATARPDIIDRNGQVLATDIKSPSLFGEPRRLIDRDEAVELLTATL
CCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHCHHHHHHHHHHHC
PDLETSEVRQRLGTRKGFVWLKREITPAQQKEIHSLGIPGIGFLRENKRVYPSGREVAHL
CCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEECCCEECCCCHHHHHH
IGLVNIDNQGIAGMEKWLDSNGLADLHRAGFATDRLQKPVELSIDLRVEHALRDELLKAK
HEEEEECCCCCHHHHHHHCCCCCHHHHHCCCCHHHHCCCCEEEEEEEHHHHHHHHHHHHH
DHYRAKAASGIVSNVRTGEIVAMVSLPDFDPNNPKEAHDPDRINRLTTGVYEMGSTFKAF
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHH
TLAMALDSGRYGLNSMWDARAPLRYGRFTIHDDHSMGRFINMKEVFTYSSNIGAARIALA
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECHHHHHEECCCCCHHHHHHH
QGVEAHKAFLRRMGQMDRLRTELPESASPILPRKWGELNTVTIAFGHGMAVAPLQAVMGI
HCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHH
DALVNGGWLIPPTFLKRTEEEAMAVARRVIKQETSDKMRFLLRLNAEVGTARKADVKGYY
HHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHEECCCCCCCCCCCCCEEE
VGGKTGTSEKVINGRYAKKRVLNSFTAILPADNPRYQILVMLDEPQPLKETHGFITSGWN
ECCCCCCCCHHHCCHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCHHHHCCCEECCCC
AVPTGGKVIARIAPLLGVQPRFDLPPSERLILAASRATQ
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]