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The map label for this gene is potI [H]

Identifier: 75675201

GI number: 75675201

Start: 1103257

End: 1104078

Strand: Direct

Name: potI [H]

Synonym: Nwi_1008

Alternate gene names: 75675201

Gene position: 1103257-1104078 (Clockwise)

Preceding gene: 75675200

Following gene: 75675203

Centisome position: 32.43

GC content: 62.77

Gene sequence:

>822_bases
ATGATGGCGAGGCCGGCGCTCGCCAGACTGTCGCCGTTCAACATTACGTCGCTCGCGCTTGGTCTGGCGTTTCTGTACCT
TCCTATCGTCATTCTCGTGATCTATTCGTTCAACGCCTCGCGGCTGGTGACGGTCTGGGGCGGATGGTCGTTGCGCTGGT
ACGTGGAGTTCTTCAACGACGAGGCCATGTTGTCCGCGCTTGCGATGAGCCTGCGAGTCGCCGCGATGTCGGCCACGGCT
GCGACCATTCTGGGAACGCTGGCCGCGGTCGCCCTGACGCGCGCGGGGTCCTTCCATGGACGAACGCTGTTTTCGGGAAT
GCTGTACGCGCCGCTGGTGATGCCCGAGGTGATCACCGGTTTGTCCCTGCTGCTGCTGTTCGTTGCGGTCGATGCGCAGC
GCGGGTTCTGGACGGTCACGATCGCGCATACGACGCTGACCATGTGCTTCGTGACCGTGGTGGTGCAGTCACGCCTCGGT
TCGCTCGATCGAAGCCTGGAGGAGGCCGCGATGGACCTCGGCTGCGATCCCTTCTGGGCGTTCGTCAAGGTGACGTTGCC
GCTGATCCTGCCATCGGTCGTCGCGGGCTGGATGCTTGCCTTTACGCTGTCGATGGACGACGTCGTGATCGCGAGTTTCA
CCACCGGACCGGGCTCGACGACGCTCCCGATCCGGATTTATTCGGAGGTGCGCCTCGGCGTGAAGCCTGAGATCAATGCG
ATCTGCACATTGATCGTCGGATCGATCGCCGTGGTCATCGTGGTCGCGTCGCTGGTCTCGAAACTCTCAAGCCGGCAGGG
CGAAAACGCAGCGCCCTTGTAG

Upstream 100 bases:

>100_bases
GGCCGGTGGCGTCGTCGATCGCCGTCGTGCTGCTGGTGCTGCTGCTGGCGCCGCTTCTTGTTTACGAGCGCGTGCAGCGT
CGCCGGTTGGAAGGGACGCG

Downstream 100 bases:

>100_bases
GCGCCGGATCGAGGAACGGCTTGGGATTGACGAAGTGTCTGCGGAGAAAACTCAGTTCGAATGCGATGTCCCGGACCCGC
TCGCGGCATCGGGGACGGCT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MMARPALARLSPFNITSLALGLAFLYLPIVILVIYSFNASRLVTVWGGWSLRWYVEFFNDEAMLSALAMSLRVAAMSATA
ATILGTLAAVALTRAGSFHGRTLFSGMLYAPLVMPEVITGLSLLLLFVAVDAQRGFWTVTIAHTTLTMCFVTVVVQSRLG
SLDRSLEEAAMDLGCDPFWAFVKVTLPLILPSVVAGWMLAFTLSMDDVVIASFTTGPGSTTLPIRIYSEVRLGVKPEINA
ICTLIVGSIAVVIVVASLVSKLSSRQGENAAPL

Sequences:

>Translated_273_residues
MMARPALARLSPFNITSLALGLAFLYLPIVILVIYSFNASRLVTVWGGWSLRWYVEFFNDEAMLSALAMSLRVAAMSATA
ATILGTLAAVALTRAGSFHGRTLFSGMLYAPLVMPEVITGLSLLLLFVAVDAQRGFWTVTIAHTTLTMCFVTVVVQSRLG
SLDRSLEEAAMDLGCDPFWAFVKVTLPLILPSVVAGWMLAFTLSMDDVVIASFTTGPGSTTLPIRIYSEVRLGVKPEINA
ICTLIVGSIAVVIVVASLVSKLSSRQGENAAPL
>Mature_273_residues
MMARPALARLSPFNITSLALGLAFLYLPIVILVIYSFNASRLVTVWGGWSLRWYVEFFNDEAMLSALAMSLRVAAMSATA
ATILGTLAAVALTRAGSFHGRTLFSGMLYAPLVMPEVITGLSLLLLFVAVDAQRGFWTVTIAHTTLTMCFVTVVVQSRLG
SLDRSLEEAAMDLGCDPFWAFVKVTLPLILPSVVAGWMLAFTLSMDDVVIASFTTGPGSTTLPIRIYSEVRLGVKPEINA
ICTLIVGSIAVVIVVASLVSKLSSRQGENAAPL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=262, Percent_Identity=55.3435114503817, Blast_Score=258, Evalue=2e-70,
Organism=Escherichia coli, GI1787368, Length=237, Percent_Identity=43.0379746835443, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787714, Length=252, Percent_Identity=33.7301587301587, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788764, Length=181, Percent_Identity=30.939226519337, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29252; Mature: 29252

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMARPALARLSPFNITSLALGLAFLYLPIVILVIYSFNASRLVTVWGGWSLRWYVEFFND
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCC
EAMLSALAMSLRVAAMSATAATILGTLAAVALTRAGSFHGRTLFSGMLYAPLVMPEVITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LSLLLLFVAVDAQRGFWTVTIAHTTLTMCFVTVVVQSRLGSLDRSLEEAAMDLGCDPFWA
HHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FVKVTLPLILPSVVAGWMLAFTLSMDDVVIASFTTGPGSTTLPIRIYSEVRLGVKPEINA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCHHH
ICTLIVGSIAVVIVVASLVSKLSSRQGENAAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMARPALARLSPFNITSLALGLAFLYLPIVILVIYSFNASRLVTVWGGWSLRWYVEFFND
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEEECCC
EAMLSALAMSLRVAAMSATAATILGTLAAVALTRAGSFHGRTLFSGMLYAPLVMPEVITG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LSLLLLFVAVDAQRGFWTVTIAHTTLTMCFVTVVVQSRLGSLDRSLEEAAMDLGCDPFWA
HHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FVKVTLPLILPSVVAGWMLAFTLSMDDVVIASFTTGPGSTTLPIRIYSEVRLGVKPEINA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEEEEECCCCCCCHHH
ICTLIVGSIAVVIVVASLVSKLSSRQGENAAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]