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The map label for this gene is potH [H]

Identifier: 75675200

GI number: 75675200

Start: 1102346

End: 1103260

Strand: Direct

Name: potH [H]

Synonym: Nwi_1007

Alternate gene names: 75675200

Gene position: 1102346-1103260 (Clockwise)

Preceding gene: 75675199

Following gene: 75675201

Centisome position: 32.4

GC content: 63.61

Gene sequence:

>915_bases
ATGAGCGGGCGGCGTCTTTTCTCCGCTCCCGCGCGCAGGGCGGCGATCGCGCCGTTTCTGTGGATGCTGCTGTTTTTCCT
GCTGCCGTTCGGCTTCGTGCTGAAGATCAGCCTGTCGCACGCGGTGATGGCGCAGCCGCCTTATCTTCCCGTGTTCGATC
CGGCCGAGGGCATTGCTGCTCCCAAGGCGGCCATCGCGGCGTTTTCGCTCGACAATTTCAGGCTGCTTGCGTCCGATCAT
TTTTACGTCCTGTCCTACCTGCGGAGCGTCGTGGTCGCCCTGGTCTCGACCTCGATCCTGCTCGCGGTCGGCTATCCCAT
CGCCTACGGCATGTCGCGCCTGCCGGCACGCTGGCAGCCGATTGCGATGATCCTGGTGATCGTGCCGTTCTGGACCTCGT
TCTTGATCCGCGTGTACGCCTGGATCAATATTCTCCAGCACGACGGCATCCTGAACAACGTGCTGATGGCGCTGCGCCTC
ATCAGCGCGCCCGTCGTCTGGCTGTCCACCGACGCGGCGATGTATATCGGGCTGGTCTATTCCTACCTGCCTTTCATGAT
CCTGCCGCTCTACGCGACATTGGCGAAAATCGATCCCGCGCTTCTTGAGGCTTCCGCCGACCTCGGCGGCTCGCGCCTGA
CCACTTTCTGGCTGGTGACGGTGCCGCTGTCGCTGCCGGGGATCGGCGCGGGCGCGCTGCTGTGCTTCATTCCGATCGTC
GGCGAGTTCGTGATTCCGGATCTGCTCGCGGGGTCGGCGTCGCCGATGATCGGGCAGACGCTGTGGCTGGAATTCTTCAA
CAACCACGATTGGCCGGTGGCGTCGTCGATCGCCGTCGTGCTGCTGGTGCTGCTGCTGGCGCCGCTTCTTGTTTACGAGC
GCGTGCAGCGTCGCCGGTTGGAAGGGACGCGATGA

Upstream 100 bases:

>100_bases
CGTCGGTCGCCAACACCGTTCGGCGGGAGGCCGAAGCCTTCGATACGGCGCAGCGCGTGGTGGCGTGTTTCGCGCCGGAC
GATTGCATGGTGCTTGACCG

Downstream 100 bases:

>100_bases
TGGCGAGGCCGGCGCTCGCCAGACTGTCGCCGTTCAACATTACGTCGCTCGCGCTTGGTCTGGCGTTTCTGTACCTTCCT
ATCGTCATTCTCGTGATCTA

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSGRRLFSAPARRAAIAPFLWMLLFFLLPFGFVLKISLSHAVMAQPPYLPVFDPAEGIAAPKAAIAAFSLDNFRLLASDH
FYVLSYLRSVVVALVSTSILLAVGYPIAYGMSRLPARWQPIAMILVIVPFWTSFLIRVYAWINILQHDGILNNVLMALRL
ISAPVVWLSTDAAMYIGLVYSYLPFMILPLYATLAKIDPALLEASADLGGSRLTTFWLVTVPLSLPGIGAGALLCFIPIV
GEFVIPDLLAGSASPMIGQTLWLEFFNNHDWPVASSIAVVLLVLLLAPLLVYERVQRRRLEGTR

Sequences:

>Translated_304_residues
MSGRRLFSAPARRAAIAPFLWMLLFFLLPFGFVLKISLSHAVMAQPPYLPVFDPAEGIAAPKAAIAAFSLDNFRLLASDH
FYVLSYLRSVVVALVSTSILLAVGYPIAYGMSRLPARWQPIAMILVIVPFWTSFLIRVYAWINILQHDGILNNVLMALRL
ISAPVVWLSTDAAMYIGLVYSYLPFMILPLYATLAKIDPALLEASADLGGSRLTTFWLVTVPLSLPGIGAGALLCFIPIV
GEFVIPDLLAGSASPMIGQTLWLEFFNNHDWPVASSIAVVLLVLLLAPLLVYERVQRRRLEGTR
>Mature_303_residues
SGRRLFSAPARRAAIAPFLWMLLFFLLPFGFVLKISLSHAVMAQPPYLPVFDPAEGIAAPKAAIAAFSLDNFRLLASDHF
YVLSYLRSVVVALVSTSILLAVGYPIAYGMSRLPARWQPIAMILVIVPFWTSFLIRVYAWINILQHDGILNNVLMALRLI
SAPVVWLSTDAAMYIGLVYSYLPFMILPLYATLAKIDPALLEASADLGGSRLTTFWLVTVPLSLPGIGAGALLCFIPIVG
EFVIPDLLAGSASPMIGQTLWLEFFNNHDWPVASSIAVVLLVLLLAPLLVYERVQRRRLEGTR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=264, Percent_Identity=47.7272727272727, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1787369, Length=232, Percent_Identity=42.2413793103448, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787713, Length=229, Percent_Identity=31.4410480349345, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33470; Mature: 33339

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRRLFSAPARRAAIAPFLWMLLFFLLPFGFVLKISLSHAVMAQPPYLPVFDPAEGIAA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PKAAIAAFSLDNFRLLASDHFYVLSYLRSVVVALVSTSILLAVGYPIAYGMSRLPARWQP
CHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHH
IAMILVIVPFWTSFLIRVYAWINILQHDGILNNVLMALRLISAPVVWLSTDAAMYIGLVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHH
SYLPFMILPLYATLAKIDPALLEASADLGGSRLTTFWLVTVPLSLPGIGAGALLCFIPIV
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHH
GEFVIPDLLAGSASPMIGQTLWLEFFNNHDWPVASSIAVVLLVLLLAPLLVYERVQRRRL
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EGTR
CCCC
>Mature Secondary Structure 
SGRRLFSAPARRAAIAPFLWMLLFFLLPFGFVLKISLSHAVMAQPPYLPVFDPAEGIAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PKAAIAAFSLDNFRLLASDHFYVLSYLRSVVVALVSTSILLAVGYPIAYGMSRLPARWQP
CHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHH
IAMILVIVPFWTSFLIRVYAWINILQHDGILNNVLMALRLISAPVVWLSTDAAMYIGLVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHH
SYLPFMILPLYATLAKIDPALLEASADLGGSRLTTFWLVTVPLSLPGIGAGALLCFIPIV
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHH
GEFVIPDLLAGSASPMIGQTLWLEFFNNHDWPVASSIAVVLLVLLLAPLLVYERVQRRRL
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EGTR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]