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The map label for this gene is proB

Identifier: 75674641

GI number: 75674641

Start: 499266

End: 500405

Strand: Direct

Name: proB

Synonym: Nwi_0443

Alternate gene names: 75674641

Gene position: 499266-500405 (Clockwise)

Preceding gene: 75674640

Following gene: 75674642

Centisome position: 14.68

GC content: 66.93

Gene sequence:

>1140_bases
ATGACCCGTCCGCATCTCAAGAAATTTCGTCGTATCGTCGTCAAGGTCGGCTCGTCGCTGCTGATCGATTCCGCGGCGGG
CAAGGTGCGGGGGGAGTGGCTGTCGGCGCTGGCCGCCGATATCGCAGGGCTTCACGGCGACGGCTGCGACGTGCTGGTGG
TCTCGTCCGGCGCGGTCGCGCTCGGCCGCAGCAAGCTCAAATTGCCTCGCGGTCCGCTGAAGCTGGAGGAGAGTCAGGCG
GCCGCCGCCGTCGGACAGATCGCGCTGGCCCGGATATGGTCGAAGGTGCTGGCCGATCACGGCATCGGCGCGGGGCAGAT
CCTGGTGACATGGCAGGACACCGAGGAGCGCCGCCGCTATCTCAATGCGCGCTCGACCATCGCGAAGCTGCTCGAATGGC
GCGCGGTCCCGGTCATCAACGAAAACGATACGGTCGCCACCAACGAAATCCGCTACGGCGACAATGACCGCCTTGCCGCG
AGGGTCGCCACCATGGCGAGCGCGGATCTGCTGATTCTGCTGTCCGACATCGACGGGCTCTATGACGCGCCGCCTCATCT
CAACCCGGATGCAAAACTGATTACGGTGGTGAAAAGGGTCACGGCGGACATCGAGGCGATGGCCGGCAGCGCCGCATCCG
AGCTGTCGCGCGGTGGAATGCGGACCAAGATCGAGGCAGCGAAGATCGCAACGACCGCCGGCACGCACATGCTGATCGCT
TCAGGCACGATCGAGCATCCGCTGCGGGCCATCATGGATGGAGGTCCCTGCACATGGTTTCTGACGCCGGCCAATCCGGT
CACGGCGCGCAAGCGTTGGATCGCCGGTTCGCTGGAGCCCAGGGGGACGCTCGCGATCGATGCCGGCGCGGTGGCGGCGC
TGCGCGCCGGCAAGAGCCTGCTTCCGGCGGGCGTGACCCGGATTGACGGGCATTTCGCGCGGGGCGATGCCGTCATCGTG
CGCGGTCCGAACGGGCATGAGATCGGCCGCGGCCTCGTCGCCTACGACGCCGCCGACGCCGACAGGATCAAGGGGCGTTC
GTCTTCCGATGCCGCGCAGCTTCTGGGGGTTCGCGGCCGCGTCGAAATGATTCATCGCGACGACCTCGTGGTCGGCGGGC
CACTCGGCGATTCCGCTTAG

Upstream 100 bases:

>100_bases
GGTTTTTCGATCGAATGATTTCGATCGGCATTCCGCTTTCCCGGCCGCCGCTTTCATGTGATGAAAGCGGCGCTTTCGTT
TCGATTGACCGTCGATTGTC

Downstream 100 bases:

>100_bases
AGCATGATCCCGACCCGAAGGACCGCGCCGGCGCAAAGTGGAAACCGGTTTTCGGAAATGATCATACTCGAACAGAAAGA
CAAATAGCGGTCTGGTTCAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTRPHLKKFRRIVVKVGSSLLIDSAAGKVRGEWLSALAADIAGLHGDGCDVLVVSSGAVALGRSKLKLPRGPLKLEESQA
AAAVGQIALARIWSKVLADHGIGAGQILVTWQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAA
RVATMASADLLILLSDIDGLYDAPPHLNPDAKLITVVKRVTADIEAMAGSAASELSRGGMRTKIEAAKIATTAGTHMLIA
SGTIEHPLRAIMDGGPCTWFLTPANPVTARKRWIAGSLEPRGTLAIDAGAVAALRAGKSLLPAGVTRIDGHFARGDAVIV
RGPNGHEIGRGLVAYDAADADRIKGRSSSDAAQLLGVRGRVEMIHRDDLVVGGPLGDSA

Sequences:

>Translated_379_residues
MTRPHLKKFRRIVVKVGSSLLIDSAAGKVRGEWLSALAADIAGLHGDGCDVLVVSSGAVALGRSKLKLPRGPLKLEESQA
AAAVGQIALARIWSKVLADHGIGAGQILVTWQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAA
RVATMASADLLILLSDIDGLYDAPPHLNPDAKLITVVKRVTADIEAMAGSAASELSRGGMRTKIEAAKIATTAGTHMLIA
SGTIEHPLRAIMDGGPCTWFLTPANPVTARKRWIAGSLEPRGTLAIDAGAVAALRAGKSLLPAGVTRIDGHFARGDAVIV
RGPNGHEIGRGLVAYDAADADRIKGRSSSDAAQLLGVRGRVEMIHRDDLVVGGPLGDSA
>Mature_378_residues
TRPHLKKFRRIVVKVGSSLLIDSAAGKVRGEWLSALAADIAGLHGDGCDVLVVSSGAVALGRSKLKLPRGPLKLEESQAA
AAVGQIALARIWSKVLADHGIGAGQILVTWQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAAR
VATMASADLLILLSDIDGLYDAPPHLNPDAKLITVVKRVTADIEAMAGSAASELSRGGMRTKIEAAKIATTAGTHMLIAS
GTIEHPLRAIMDGGPCTWFLTPANPVTARKRWIAGSLEPRGTLAIDAGAVAALRAGKSLLPAGVTRIDGHFARGDAVIVR
GPNGHEIGRGLVAYDAADADRIKGRSSSDAAQLLGVRGRVEMIHRDDLVVGGPLGDSA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=314, Percent_Identity=32.1656050955414, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI62912457, Length=312, Percent_Identity=32.0512820512821, Blast_Score=131, Evalue=9e-31,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=40.2203856749311, Blast_Score=245, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17569967, Length=257, Percent_Identity=33.4630350194552, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=257, Percent_Identity=33.4630350194552, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=31.4903846153846, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=30.6954436450839, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21357643, Length=293, Percent_Identity=31.740614334471, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_NITWN (Q3SVI1)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317062.1
- ProteinModelPortal:   Q3SVI1
- SMR:   Q3SVI1
- STRING:   Q3SVI1
- GeneID:   3676618
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0443
- NMPDR:   fig|323098.3.peg.135
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q3SVI1
- ProtClustDB:   PRK05429
- BioCyc:   NWIN323098:NWI_0443-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39934; Mature: 39803

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPHLKKFRRIVVKVGSSLLIDSAAGKVRGEWLSALAADIAGLHGDGCDVLVVSSGAVA
CCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEE
LGRSKLKLPRGPLKLEESQAAAAVGQIALARIWSKVLADHGIGAGQILVTWQDTEERRRY
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHH
LNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAARVATMASADLLILLSDIDGL
HHHHHHHHHHHHHCCCEEECCCCCEEECEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
YDAPPHLNPDAKLITVVKRVTADIEAMAGSAASELSRGGMRTKIEAAKIATTAGTHMLIA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHEEECCCCEEEEE
SGTIEHPLRAIMDGGPCTWFLTPANPVTARKRWIAGSLEPRGTLAIDAGAVAALRAGKSL
CCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHEECCCCCCCEEEECCCHHHHHHCCCCC
LPAGVTRIDGHFARGDAVIVRGPNGHEIGRGLVAYDAADADRIKGRSSSDAAQLLGVRGR
CCCCHHHCCCEEECCCEEEEECCCCHHHCCCEEEECCCCHHHHCCCCCCCHHHHHCCCCC
VEMIHRDDLVVGGPLGDSA
EEEEECCCEEEECCCCCCC
>Mature Secondary Structure 
TRPHLKKFRRIVVKVGSSLLIDSAAGKVRGEWLSALAADIAGLHGDGCDVLVVSSGAVA
CCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEE
LGRSKLKLPRGPLKLEESQAAAAVGQIALARIWSKVLADHGIGAGQILVTWQDTEERRRY
ECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHH
LNARSTIAKLLEWRAVPVINENDTVATNEIRYGDNDRLAARVATMASADLLILLSDIDGL
HHHHHHHHHHHHHCCCEEECCCCCEEECEEEECCCCHHHHHHHHHCCCCEEEEEECCCCC
YDAPPHLNPDAKLITVVKRVTADIEAMAGSAASELSRGGMRTKIEAAKIATTAGTHMLIA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHEEECCCCEEEEE
SGTIEHPLRAIMDGGPCTWFLTPANPVTARKRWIAGSLEPRGTLAIDAGAVAALRAGKSL
CCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHEECCCCCCCEEEECCCHHHHHHCCCCC
LPAGVTRIDGHFARGDAVIVRGPNGHEIGRGLVAYDAADADRIKGRSSSDAAQLLGVRGR
CCCCHHHCCCEEECCCEEEEECCCCHHHCCCEEEECCCCHHHHCCCCCCCHHHHHCCCCC
VEMIHRDDLVVGGPLGDSA
EEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA