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The map label for this gene is obgE [H]

Identifier: 75674640

GI number: 75674640

Start: 498050

End: 499126

Strand: Direct

Name: obgE [H]

Synonym: Nwi_0442

Alternate gene names: 75674640

Gene position: 498050-499126 (Clockwise)

Preceding gene: 75674639

Following gene: 75674641

Centisome position: 14.64

GC content: 65.92

Gene sequence:

>1077_bases
ATGAAATTTCTCGATGAAGCCAAGGTCTATATCCGTTCCGGCGATGGTGGTAACGGCTGCGTGGCTTTCCGCCGCGAGAA
GTTTATCGAGTTCGGGGGCCCCTCCGGCGGCAATGGCGGCCGCGGCGGCGACGTCATCGTCGAGGTCGCCGATGGCCTCA
ACACCCTGATCGACTATCGCTACCAGCAGCACTTCAAGGCTCCCAAGGGCGCCAACGGCATGGGCTCGGACCGCCACGGC
GCGAACGGCAAGGCCATCGTGCTCAAGGTGCCGCTTGGCACCCAGATCATCGACGAGGATCGCGAAACCCTGATCCACGA
CTTCACCCGGGTCGGCGAGCGCCTTGTGCTGGCCGAGGGCGGCAATGGCGGGTTCGGCAACGCGCACTTCAAGTCGTCGA
CCAACCGGGCGCCGCGCCGCGCCAACCCCGGCCAGCCGGGCGAGGAGCGCTGGATCTGGCTGCGGTTGAAGCTGATTGCC
GATGCCGGTCTGGTCGGCCTGCCCAATGCAGGCAAGTCGACGTTTCTTTCCAAGGTGAGTGCGGCGAAGCCGAAGATCGC
CGACTATCCGTTTACCACGCTGCACCCGCAACTCGGCGTCGTGAGAGTGCAGGATCGCGAATTCGTGCTGGCGGATATTC
CCGGATTGATTGAGGGCGCGCATGAGGGCGCGGGACTGGGCGACCGTTTCCTCGGGCATGTCGAGCGCTGCCGCGTGCTG
CTGCATCTGGTGGATGCCGGCTGTGACCATGCCGGCCGCGCCTACAAGATCGTGCGCGGAGAAATGGAAGCCTACGCCGG
GGCGCTTGCCGAGAAGGTCGAGATCGTGGCGCTCAACAAGATCGACGCGGTCACGGCCGAGGATCTGAAGAAGCAGCGCG
ACCGCCTCAAGCGCGCCGCGAAAAAAACGCCGCTGCTGGTCTCAGGCGTCACGGGCGAGGGCGTGCAGGATGTGCTGCGC
GCGCTGGTGGAGGTCATCGGGCAGGCGCCGGTCTCGGACAAGGCCAAGGGCGCGGATGCTTCGGCGGCACAGGCGATGGA
AACGCCGGTCGCCCGGGCCAAGCCGTGGTCACCCTGA

Upstream 100 bases:

>100_bases
AGGCTTCGCTCGGAACGCGTTCACTCTCCTTTCAGGCGACGGCCGTGATGCCACGCGATGGGCCGTTGCCTCCCTGGCTT
CCCTATCCTACCTATCAGCC

Downstream 100 bases:

>100_bases
GCCGCGGCGGTGAGCGCATACTTCCTATAAAAGGGATACGGTTTTTCGATCGAATGATTTCGATCGGCATTCCGCTTTCC
CGGCCGCCGCTTTCATGTGA

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGDVIVEVADGLNTLIDYRYQQHFKAPKGANGMGSDRHG
ANGKAIVLKVPLGTQIIDEDRETLIHDFTRVGERLVLAEGGNGGFGNAHFKSSTNRAPRRANPGQPGEERWIWLRLKLIA
DAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVRVQDREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
LHLVDAGCDHAGRAYKIVRGEMEAYAGALAEKVEIVALNKIDAVTAEDLKKQRDRLKRAAKKTPLLVSGVTGEGVQDVLR
ALVEVIGQAPVSDKAKGADASAAQAMETPVARAKPWSP

Sequences:

>Translated_358_residues
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGDVIVEVADGLNTLIDYRYQQHFKAPKGANGMGSDRHG
ANGKAIVLKVPLGTQIIDEDRETLIHDFTRVGERLVLAEGGNGGFGNAHFKSSTNRAPRRANPGQPGEERWIWLRLKLIA
DAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVRVQDREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
LHLVDAGCDHAGRAYKIVRGEMEAYAGALAEKVEIVALNKIDAVTAEDLKKQRDRLKRAAKKTPLLVSGVTGEGVQDVLR
ALVEVIGQAPVSDKAKGADASAAQAMETPVARAKPWSP
>Mature_358_residues
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGDVIVEVADGLNTLIDYRYQQHFKAPKGANGMGSDRHG
ANGKAIVLKVPLGTQIIDEDRETLIHDFTRVGERLVLAEGGNGGFGNAHFKSSTNRAPRRANPGQPGEERWIWLRLKLIA
DAGLVGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPQLGVVRVQDREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
LHLVDAGCDHAGRAYKIVRGEMEAYAGALAEKVEIVALNKIDAVTAEDLKKQRDRLKRAAKKTPLLVSGVTGEGVQDVLR
ALVEVIGQAPVSDKAKGADASAAQAMETPVARAKPWSP

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI24308117, Length=324, Percent_Identity=39.1975308641975, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI111955139, Length=346, Percent_Identity=36.7052023121387, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI111955063, Length=180, Percent_Identity=38.8888888888889, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI58761500, Length=147, Percent_Identity=31.9727891156463, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI4758796, Length=123, Percent_Identity=34.1463414634146, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789574, Length=338, Percent_Identity=50.5917159763314, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1787454, Length=102, Percent_Identity=36.2745098039216, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790615, Length=171, Percent_Identity=31.5789473684211, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508313, Length=324, Percent_Identity=37.6543209876543, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17552324, Length=305, Percent_Identity=31.4754098360656, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17509631, Length=105, Percent_Identity=38.0952380952381, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17555344, Length=87, Percent_Identity=37.9310344827586, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321962, Length=159, Percent_Identity=41.5094339622642, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319281, Length=89, Percent_Identity=37.0786516853933, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321612, Length=91, Percent_Identity=37.3626373626374, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20129375, Length=325, Percent_Identity=36.3076923076923, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24585318, Length=298, Percent_Identity=36.5771812080537, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24640873, Length=104, Percent_Identity=35.5769230769231, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640877, Length=104, Percent_Identity=35.5769230769231, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640875, Length=104, Percent_Identity=35.5769230769231, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17981711, Length=113, Percent_Identity=32.7433628318584, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014100
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917 [H]

Pfam domain/function: PF01018 GTP1_OBG; PF01926 MMR_HSR1 [H]

EC number: NA

Molecular weight: Translated: 38264; Mature: 38264

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGDVIVEVADGLNTLIDYR
CCCCCCCEEEEEECCCCCCEEEEHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
YQQHFKAPKGANGMGSDRHGANGKAIVLKVPLGTQIIDEDRETLIHDFTRVGERLVLAEG
HHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEC
GNGGFGNAHFKSSTNRAPRRANPGQPGEERWIWLRLKLIADAGLVGLPNAGKSTFLSKVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHH
AAKPKIADYPFTTLHPQLGVVRVQDREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
HCCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHH
LHLVDAGCDHAGRAYKIVRGEMEAYAGALAEKVEIVALNKIDAVTAEDLKKQRDRLKRAA
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHH
KKTPLLVSGVTGEGVQDVLRALVEVIGQAPVSDKAKGADASAAQAMETPVARAKPWSP
HCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MKFLDEAKVYIRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGDVIVEVADGLNTLIDYR
CCCCCCCEEEEEECCCCCCEEEEHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHH
YQQHFKAPKGANGMGSDRHGANGKAIVLKVPLGTQIIDEDRETLIHDFTRVGERLVLAEG
HHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEC
GNGGFGNAHFKSSTNRAPRRANPGQPGEERWIWLRLKLIADAGLVGLPNAGKSTFLSKVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHH
AAKPKIADYPFTTLHPQLGVVRVQDREFVLADIPGLIEGAHEGAGLGDRFLGHVERCRVL
HCCCCCCCCCCCCCCCCCCEEEECCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHHH
LHLVDAGCDHAGRAYKIVRGEMEAYAGALAEKVEIVALNKIDAVTAEDLKKQRDRLKRAA
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHH
KKTPLLVSGVTGEGVQDVLRALVEVIGQAPVSDKAKGADASAAQAMETPVARAKPWSP
HCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA