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The map label for this gene is proA [H]

Identifier: 75674642

GI number: 75674642

Start: 500732

End: 502024

Strand: Direct

Name: proA [H]

Synonym: Nwi_0444

Alternate gene names: 75674642

Gene position: 500732-502024 (Clockwise)

Preceding gene: 75674641

Following gene: 75674643

Centisome position: 14.72

GC content: 66.9

Gene sequence:

>1293_bases
ATGACGGCTCCCATGAAGGCGATCGGCGCTCCGGCCGATCTTCCCGTGTTGATGGGCGACCTCGCAGCACAGGCGCGCGA
GGCCGCGCGCTCGCTCGGGCTCGCTTCAACGGCGCAGAAGAACGAGGCGCTCGATGCGATGGCGCGCGCGATTCGAAGCA
ATGCGGCTGCGATCCTCCAGGCCAATGCGCAAGACGTCGCGGATGCACGCGCCGGCGGCGCCACCGCCGCTTTCCTCGAT
CGTCTCACGCTGACGCAGGCGCGCGTGGAGGCGATGGCCGACGGCGTGAAGGTGGTTCGCGAGATAGACGATCCGGTGGG
GCGGGTCACCGAGCGCTGGCAGCGTCCCAACGGCATGACCATCGAGCGCGTGCGGGTGCCGCTCGGAGTGGTCGCCGTGA
TCTTCGAGAGCCGTCCCAATGTCTGCGCCGACGCCGGCGTGCTCTGTCTTAAATCCGGCAACGCCGTGATCCTGCGCGGC
GGCTCGGAAAGTTTTCGCTCATGCCGCGCGATTCATGCGTGCCTGGTCGAGGGATTGCGCGAGGCGCGATTGCCTGAAGC
GGCGATCACGCTGGTGCCGACGCGCGACCGCGCCGCGGTCGGGCTGCTTCTGAGCGGGATGAATGGCGGCATCGACGTGA
TCGTGCCGCGCGGCGGCAAAAGCCTTGTCGCGCGTGTCGAGGCCGAGGCGCGCGTGCCGGTGTTCGCGCATCTTGAAGGC
GTCAATCATGTTTATGTCGATGGCGCCGCCGATCTCGCAATGGCGAAGGCGGTCGTGCTCAACTCGAAAATGCGCCGCCC
CGGCGTTTGCGGTGCGGCCGAAACGCTGCTGGTCGATCGCTCGGGCGTACGGGATATTCTGAAGCCGCTGGTCGAAGCGC
TGATCGAGGCCGGTTGCGAGGTGCGCGGCGATTCCGACGTGCAGGGTGTCGATCCGCGGGTCAGGTCCGCGGCCGACAGC
GACTGGAATACCGAATACGGCGACGCGATCATTTCCGCGCGCGTGGTCGACGGTTGCGGCGGCGCGATTGATCATATCCA
GCGTCACGGCTCGCGCCACACGGATGCGATCGTGACCGAGGATCCTGAAACGGCGGCCCGGTTTCTCAACGAGGTCGATT
CGGCGATCGTGCTGCACAATGCGTCGACGCAATTCGCCGACGGCGGGGAGTTCGGCTTCGGGGCTGAAATCGGCATTGCC
ACGGGCAAGTTCCACGCGCGGGGGCCGGTTGGCGCCGAGCAGTTGACGAGCTTCAAATATCGCGTCCATGGTGACGGGCA
GGTGCGCCCGTGA

Upstream 100 bases:

>100_bases
CAGCCTCGGTCTTGCGAGGACAGTCGTCTCACGCCTCGCCGGGCCCGGATTTATATGTTAGGGATATGACAAGCACATAC
GATCGAAGACTGTTACCCGA

Downstream 100 bases:

>100_bases
GCGCGCCCGGATCATCCTGCGGGTGGCGCGGGGATTGATTTGGGGTCGCGTTCCGTCGTCTCACGCGCCATCCCGCTTCA
TTCCGATGGCATGCGTATCG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTAPMKAIGAPADLPVLMGDLAAQAREAARSLGLASTAQKNEALDAMARAIRSNAAAILQANAQDVADARAGGATAAFLD
RLTLTQARVEAMADGVKVVREIDDPVGRVTERWQRPNGMTIERVRVPLGVVAVIFESRPNVCADAGVLCLKSGNAVILRG
GSESFRSCRAIHACLVEGLREARLPEAAITLVPTRDRAAVGLLLSGMNGGIDVIVPRGGKSLVARVEAEARVPVFAHLEG
VNHVYVDGAADLAMAKAVVLNSKMRRPGVCGAAETLLVDRSGVRDILKPLVEALIEAGCEVRGDSDVQGVDPRVRSAADS
DWNTEYGDAIISARVVDGCGGAIDHIQRHGSRHTDAIVTEDPETAARFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIA
TGKFHARGPVGAEQLTSFKYRVHGDGQVRP

Sequences:

>Translated_430_residues
MTAPMKAIGAPADLPVLMGDLAAQAREAARSLGLASTAQKNEALDAMARAIRSNAAAILQANAQDVADARAGGATAAFLD
RLTLTQARVEAMADGVKVVREIDDPVGRVTERWQRPNGMTIERVRVPLGVVAVIFESRPNVCADAGVLCLKSGNAVILRG
GSESFRSCRAIHACLVEGLREARLPEAAITLVPTRDRAAVGLLLSGMNGGIDVIVPRGGKSLVARVEAEARVPVFAHLEG
VNHVYVDGAADLAMAKAVVLNSKMRRPGVCGAAETLLVDRSGVRDILKPLVEALIEAGCEVRGDSDVQGVDPRVRSAADS
DWNTEYGDAIISARVVDGCGGAIDHIQRHGSRHTDAIVTEDPETAARFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIA
TGKFHARGPVGAEQLTSFKYRVHGDGQVRP
>Mature_429_residues
TAPMKAIGAPADLPVLMGDLAAQAREAARSLGLASTAQKNEALDAMARAIRSNAAAILQANAQDVADARAGGATAAFLDR
LTLTQARVEAMADGVKVVREIDDPVGRVTERWQRPNGMTIERVRVPLGVVAVIFESRPNVCADAGVLCLKSGNAVILRGG
SESFRSCRAIHACLVEGLREARLPEAAITLVPTRDRAAVGLLLSGMNGGIDVIVPRGGKSLVARVEAEARVPVFAHLEGV
NHVYVDGAADLAMAKAVVLNSKMRRPGVCGAAETLLVDRSGVRDILKPLVEALIEAGCEVRGDSDVQGVDPRVRSAADSD
WNTEYGDAIISARVVDGCGGAIDHIQRHGSRHTDAIVTEDPETAARFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIAT
GKFHARGPVGAEQLTSFKYRVHGDGQVRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=412, Percent_Identity=37.8640776699029, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI21361368, Length=412, Percent_Identity=37.8640776699029, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=40.5275779376499, Blast_Score=311, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI17569967, Length=416, Percent_Identity=36.0576923076923, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17569969, Length=416, Percent_Identity=36.0576923076923, Blast_Score=251, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6324899, Length=431, Percent_Identity=36.8909512761021, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI21357643, Length=411, Percent_Identity=37.2262773722628, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45119; Mature: 44988

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPMKAIGAPADLPVLMGDLAAQAREAARSLGLASTAQKNEALDAMARAIRSNAAAILQ
CCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
ANAQDVADARAGGATAAFLDRLTLTQARVEAMADGVKVVREIDDPVGRVTERWQRPNGMT
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
IERVRVPLGVVAVIFESRPNVCADAGVLCLKSGNAVILRGGSESFRSCRAIHACLVEGLR
HHEECCCHHHEEHEECCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
EARLPEAAITLVPTRDRAAVGLLLSGMNGGIDVIVPRGGKSLVARVEAEARVPVFAHLEG
HHCCCCCEEEEEECCCCHHHHHHEECCCCCEEEEEECCCHHHEEEECCCCCCCEEEEECC
VNHVYVDGAADLAMAKAVVLNSKMRRPGVCGAAETLLVDRSGVRDILKPLVEALIEAGCE
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCC
VRGDSDVQGVDPRVRSAADSDWNTEYGDAIISARVVDGCGGAIDHIQRHGSRHTDAIVTE
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHEEHHHCCCCHHHHHHHHCCCCCCCCEEEC
DPETAARFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGAEQLTSFKY
CHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCEEE
RVHGDGQVRP
EECCCCCCCC
>Mature Secondary Structure 
TAPMKAIGAPADLPVLMGDLAAQAREAARSLGLASTAQKNEALDAMARAIRSNAAAILQ
CCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
ANAQDVADARAGGATAAFLDRLTLTQARVEAMADGVKVVREIDDPVGRVTERWQRPNGMT
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
IERVRVPLGVVAVIFESRPNVCADAGVLCLKSGNAVILRGGSESFRSCRAIHACLVEGLR
HHEECCCHHHEEHEECCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHH
EARLPEAAITLVPTRDRAAVGLLLSGMNGGIDVIVPRGGKSLVARVEAEARVPVFAHLEG
HHCCCCCEEEEEECCCCHHHHHHEECCCCCEEEEEECCCHHHEEEECCCCCCCEEEEECC
VNHVYVDGAADLAMAKAVVLNSKMRRPGVCGAAETLLVDRSGVRDILKPLVEALIEAGCE
CCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCC
VRGDSDVQGVDPRVRSAADSDWNTEYGDAIISARVVDGCGGAIDHIQRHGSRHTDAIVTE
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHEEHHHCCCCHHHHHHHHCCCCCCCCEEEC
DPETAARFLNEVDSAIVLHNASTQFADGGEFGFGAEIGIATGKFHARGPVGAEQLTSFKY
CHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCEEE
RVHGDGQVRP
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA