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The map label for this gene is gltD [H]

Identifier: 74318495

GI number: 74318495

Start: 2552614

End: 2554575

Strand: Reverse

Name: gltD [H]

Synonym: Tbd_2477

Alternate gene names: 74318495

Gene position: 2554575-2552614 (Counterclockwise)

Preceding gene: 74318496

Following gene: 74318494

Centisome position: 87.79

GC content: 63.35

Gene sequence:

>1962_bases
ATGGCTGTCACGACGAACAAAGCAGCAAAGACCGAAGGCATGACGTGGCGTCGCTACAAGGATGGCGAGTCGGAAGCGAA
CATCCGTTCGGCTCAGGACCGCATTTTCCAGTCGAGCTGGACGCACAAGTGCCCAGAGTACATGCTCTCCACGCCGCCTT
GCCAGGGTTCCTGCCCGGCCGGTGAAGACATCCGCGGTTACCTCAACATCGTGCGCGGCATCGAGAAGCCGCCAGCAGGC
GTGACCTGGCAGGAGTATGCCTTCCGCCGCGTGACCGAAGCCAACCCCTTCCCGGGCGTGATGGGCCGCGTGTGTCCCGC
TCCGTGTGAATCGGGCTGTAACCGTAACATGGTCGAAGACCATGTCGGCATCAACTCGGTCGAGCACTTCATCGGCGACT
GGGGCATCGAAAACAACATGGCCTACACGTCGACCGCTGCCGAAACCGGCAAGAAGATCGCGATCATCGGCGCCGGCCCG
GCCGGTCTGGCGGCCGCCTACCAGCTGCGCCTGCGTGGACACGGCGTGACCATTTTCGACGAGCACGAAGAGCTCGGCGG
CATGATGCGCTACGGCATCCCGGGCTTCCGCACGCCGCGTGAAATGCTCGACGCGGAAATCAACCGCATCATCGCGCTGG
GCGTCGAGACCCGCCTCAAGACGCGCGTCGGCTCGGATATCAGCTTCGATGAGCTCGACAAGAACTACGACGCCATCTTC
ATGGCGATGGGCGCGCAAGCGGGTCGTCCGCTGCCCGTTCCCGGCGCCGAAGCGCCGAACTGCGTGACCGCCGTCGCGTT
CCTGCGTGCCTTCAACGAAGGTCGTCTGCAGCACGTCGGCAACCGCGTCGTCGTGATCGGTGGTGGCGACACCTCGATCG
ACGTCGCGACCGTTGCGCGCCGCCTCGGCAATGTCACAAAGTCTGGCACCCATGATGACCGTCCCGAAGCCGTGATCGCG
GGCCACATGGCTTCCGACGTCGCGTCGATTTCCGCCCGCGAGGGTGCCGAAGTGGTGCTCGTGTCGCGTGCCACGGTCGA
CAAGATGGCCGCCAACAAGCATGAAGTCGAGCACGCCCAGCAGGAAGGCATCGAAATCATCGGCGGCGTGACCCCGGTCT
CCGTCGTCGTCGACGCCAATGGCCGTGCGACCGCACTGCGCGTCGCCGACTTCGTGATGGAAGGCAAGGAAACCAAGATC
GTCGAAGGCACCGAGCGTGATCTGCCGGCCGACCTGATCGTGTCTGCGATCGGCCAAGGCGTCGACTTCACCGGCCTCGA
GCAGTTCAACAACAACAATGGCCTGATCAAGGCCGACAAGAACTACCGCTTCCCCAACAACGAGAAGGTCTTCGTCGGTG
GCGACGTGATCCGTCCGCACCTGCTGACGACCGCGATCGGCCACGCCTCGATCGCCGTCGACGGCATCGACGCCTTCCTC
GCAGGCAAGGAGCTCGACAAGCGTCCCAAGGTCGACGTTCACCACTTCGACGAAATCCGCAAGTGGCTCGAATCCGGCCA
CGAGTACAGCGAGGTCAAGGGTCAGATCTGGGGCACGTCCGAGCGCAAGGACATCCTGCACAACTTCGAGGACCGTTCGC
AGCGTCACGTCATTCCGCACAACGAGCTGTTCCTCGGCCACTTCCCCAACGAAGCGCGCCACATCCGTGAAGTGACGGTG
CTCGACAAGGAAGGTGCGCTCGGCAACTTCGAAGAGCGTCTGCACGCGCTGTCGGAGAAGGACGTGGTCGCCGAGGCCAA
GCGCTGCATGTCCTGCGGCCAGTGCTTCGAATGCGACAACTGCGTGGTGTACTGCCCGCAGACGGCCGTGTTCAAGGTCA
AGAAGAAGGACAACCCGACCGTGGGCCGTTACGTCGATACCGACTACGGCAAGTGCATCGGCTGCCACATCTGTGCCGAC
GTCTGCCCGACCGGCTACATCGTCATGGGGATGGGTGACTGA

Upstream 100 bases:

>100_bases
AGCATGGTGGGCGGGGTGCATCAATTGGTCAGCAAGGCGATCAAGCTCGACTGACCATATTTGACAACTTATTCAATTCG
TAGGCGTTAGGAGAACCAAC

Downstream 100 bases:

>100_bases
GCGTGACGCCCAAGTTCAAACGTCTGCTGGCATTGGGTGCGGCCGTCATGACGACGGCGGCCCTTGCCTGGGCCGGGAGC
GACGCCGGCAGCCCGACCAA

Product: putative glutamate synthase (NADPH) small subunit

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MAVTTNKAAKTEGMTWRRYKDGESEANIRSAQDRIFQSSWTHKCPEYMLSTPPCQGSCPAGEDIRGYLNIVRGIEKPPAG
VTWQEYAFRRVTEANPFPGVMGRVCPAPCESGCNRNMVEDHVGINSVEHFIGDWGIENNMAYTSTAAETGKKIAIIGAGP
AGLAAAYQLRLRGHGVTIFDEHEELGGMMRYGIPGFRTPREMLDAEINRIIALGVETRLKTRVGSDISFDELDKNYDAIF
MAMGAQAGRPLPVPGAEAPNCVTAVAFLRAFNEGRLQHVGNRVVVIGGGDTSIDVATVARRLGNVTKSGTHDDRPEAVIA
GHMASDVASISAREGAEVVLVSRATVDKMAANKHEVEHAQQEGIEIIGGVTPVSVVVDANGRATALRVADFVMEGKETKI
VEGTERDLPADLIVSAIGQGVDFTGLEQFNNNNGLIKADKNYRFPNNEKVFVGGDVIRPHLLTTAIGHASIAVDGIDAFL
AGKELDKRPKVDVHHFDEIRKWLESGHEYSEVKGQIWGTSERKDILHNFEDRSQRHVIPHNELFLGHFPNEARHIREVTV
LDKEGALGNFEERLHALSEKDVVAEAKRCMSCGQCFECDNCVVYCPQTAVFKVKKKDNPTVGRYVDTDYGKCIGCHICAD
VCPTGYIVMGMGD

Sequences:

>Translated_653_residues
MAVTTNKAAKTEGMTWRRYKDGESEANIRSAQDRIFQSSWTHKCPEYMLSTPPCQGSCPAGEDIRGYLNIVRGIEKPPAG
VTWQEYAFRRVTEANPFPGVMGRVCPAPCESGCNRNMVEDHVGINSVEHFIGDWGIENNMAYTSTAAETGKKIAIIGAGP
AGLAAAYQLRLRGHGVTIFDEHEELGGMMRYGIPGFRTPREMLDAEINRIIALGVETRLKTRVGSDISFDELDKNYDAIF
MAMGAQAGRPLPVPGAEAPNCVTAVAFLRAFNEGRLQHVGNRVVVIGGGDTSIDVATVARRLGNVTKSGTHDDRPEAVIA
GHMASDVASISAREGAEVVLVSRATVDKMAANKHEVEHAQQEGIEIIGGVTPVSVVVDANGRATALRVADFVMEGKETKI
VEGTERDLPADLIVSAIGQGVDFTGLEQFNNNNGLIKADKNYRFPNNEKVFVGGDVIRPHLLTTAIGHASIAVDGIDAFL
AGKELDKRPKVDVHHFDEIRKWLESGHEYSEVKGQIWGTSERKDILHNFEDRSQRHVIPHNELFLGHFPNEARHIREVTV
LDKEGALGNFEERLHALSEKDVVAEAKRCMSCGQCFECDNCVVYCPQTAVFKVKKKDNPTVGRYVDTDYGKCIGCHICAD
VCPTGYIVMGMGD
>Mature_652_residues
AVTTNKAAKTEGMTWRRYKDGESEANIRSAQDRIFQSSWTHKCPEYMLSTPPCQGSCPAGEDIRGYLNIVRGIEKPPAGV
TWQEYAFRRVTEANPFPGVMGRVCPAPCESGCNRNMVEDHVGINSVEHFIGDWGIENNMAYTSTAAETGKKIAIIGAGPA
GLAAAYQLRLRGHGVTIFDEHEELGGMMRYGIPGFRTPREMLDAEINRIIALGVETRLKTRVGSDISFDELDKNYDAIFM
AMGAQAGRPLPVPGAEAPNCVTAVAFLRAFNEGRLQHVGNRVVVIGGGDTSIDVATVARRLGNVTKSGTHDDRPEAVIAG
HMASDVASISAREGAEVVLVSRATVDKMAANKHEVEHAQQEGIEIIGGVTPVSVVVDANGRATALRVADFVMEGKETKIV
EGTERDLPADLIVSAIGQGVDFTGLEQFNNNNGLIKADKNYRFPNNEKVFVGGDVIRPHLLTTAIGHASIAVDGIDAFLA
GKELDKRPKVDVHHFDEIRKWLESGHEYSEVKGQIWGTSERKDILHNFEDRSQRHVIPHNELFLGHFPNEARHIREVTVL
DKEGALGNFEERLHALSEKDVVAEAKRCMSCGQCFECDNCVVYCPQTAVFKVKKKDNPTVGRYVDTDYGKCIGCHICADV
CPTGYIVMGMGD

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=453, Percent_Identity=26.2693156732892, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1789606, Length=461, Percent_Identity=30.1518438177874, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1788811, Length=479, Percent_Identity=29.2275574112735, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI1788468, Length=436, Percent_Identity=27.2935779816514, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI87082180, Length=500, Percent_Identity=27.8, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1789243, Length=433, Percent_Identity=25.4041570438799, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789463, Length=159, Percent_Identity=33.9622641509434, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17570289, Length=460, Percent_Identity=28.695652173913, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=279, Percent_Identity=25.8064516129032, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17543860, Length=342, Percent_Identity=24.2690058479532, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=298, Percent_Identity=31.8791946308725, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24665547, Length=455, Percent_Identity=29.8901098901099, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24665543, Length=455, Percent_Identity=29.8901098901099, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI28574881, Length=455, Percent_Identity=29.8901098901099, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24665539, Length=455, Percent_Identity=29.8901098901099, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24640763, Length=476, Percent_Identity=25.2100840336134, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18858217, Length=476, Percent_Identity=25.2100840336134, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 71333; Mature: 71201

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTTNKAAKTEGMTWRRYKDGESEANIRSAQDRIFQSSWTHKCPEYMLSTPPCQGSCPA
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
GEDIRGYLNIVRGIEKPPAGVTWQEYAFRRVTEANPFPGVMGRVCPAPCESGCNRNMVED
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCHHHCCCCCCHHHH
HVGINSVEHFIGDWGIENNMAYTSTAAETGKKIAIIGAGPAGLAAAYQLRLRGHGVTIFD
HCCHHHHHHHHHCCCCCCCEEEEEHHHHCCCEEEEEECCCHHHHHEEEEEEECCCEEEEE
EHEELGGMMRYGIPGFRTPREMLDAEINRIIALGVETRLKTRVGSDISFDELDKNYDAIF
CHHHHCCHHHCCCCCCCCHHHHHHHHHHHEEEECCHHHHHHHCCCCCCHHHHCCCCCEEE
MAMGAQAGRPLPVPGAEAPNCVTAVAFLRAFNEGRLQHVGNRVVVIGGGDTSIDVATVAR
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCHHHHHHH
RLGNVTKSGTHDDRPEAVIAGHMASDVASISAREGAEVVLVSRATVDKMAANKHEVEHAQ
HHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCHHHHHHHH
QEGIEIIGGVTPVSVVVDANGRATALRVADFVMEGKETKIVEGTERDLPADLIVSAIGQG
HCCCEEECCCCEEEEEEECCCCEEHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCC
VDFTGLEQFNNNNGLIKADKNYRFPNNEKVFVGGDVIRPHLLTTAIGHASIAVDGIDAFL
CCCCCHHHHCCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEECHHHHH
AGKELDKRPKVDVHHFDEIRKWLESGHEYSEVKGQIWGTSERKDILHNFEDRSQRHVIPH
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCEECCCCHHHHHHHHHHCCCCCCCCCC
NELFLGHFPNEARHIREVTVLDKEGALGNFEERLHALSEKDVVAEAKRCMSCGQCFECDN
CCEEEECCCCHHHHHEEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCC
CVVYCPQTAVFKVKKKDNPTVGRYVDTDYGKCIGCHICADVCPTGYIVMGMGD
EEEECCCCEEEEEECCCCCCCCCEECCCCCCEEHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure 
AVTTNKAAKTEGMTWRRYKDGESEANIRSAQDRIFQSSWTHKCPEYMLSTPPCQGSCPA
CCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
GEDIRGYLNIVRGIEKPPAGVTWQEYAFRRVTEANPFPGVMGRVCPAPCESGCNRNMVED
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCHHHCCCCCCHHHH
HVGINSVEHFIGDWGIENNMAYTSTAAETGKKIAIIGAGPAGLAAAYQLRLRGHGVTIFD
HCCHHHHHHHHHCCCCCCCEEEEEHHHHCCCEEEEEECCCHHHHHEEEEEEECCCEEEEE
EHEELGGMMRYGIPGFRTPREMLDAEINRIIALGVETRLKTRVGSDISFDELDKNYDAIF
CHHHHCCHHHCCCCCCCCHHHHHHHHHHHEEEECCHHHHHHHCCCCCCHHHHCCCCCEEE
MAMGAQAGRPLPVPGAEAPNCVTAVAFLRAFNEGRLQHVGNRVVVIGGGDTSIDVATVAR
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCHHHHHHH
RLGNVTKSGTHDDRPEAVIAGHMASDVASISAREGAEVVLVSRATVDKMAANKHEVEHAQ
HHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCHHHHHHHH
QEGIEIIGGVTPVSVVVDANGRATALRVADFVMEGKETKIVEGTERDLPADLIVSAIGQG
HCCCEEECCCCEEEEEEECCCCEEHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCC
VDFTGLEQFNNNNGLIKADKNYRFPNNEKVFVGGDVIRPHLLTTAIGHASIAVDGIDAFL
CCCCCHHHHCCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEECHHHHH
AGKELDKRPKVDVHHFDEIRKWLESGHEYSEVKGQIWGTSERKDILHNFEDRSQRHVIPH
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCEECCCCHHHHHHHHHHCCCCCCCCCC
NELFLGHFPNEARHIREVTVLDKEGALGNFEERLHALSEKDVVAEAKRCMSCGQCFECDN
CCEEEECCCCHHHHHEEEEEEECCCCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCC
CVVYCPQTAVFKVKKKDNPTVGRYVDTDYGKCIGCHICADVCPTGYIVMGMGD
EEEECCCCEEEEEECCCCCCCCCEECCCCCCEEHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]