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The map label for this gene is oprF [H]

Identifier: 74318472

GI number: 74318472

Start: 2525239

End: 2526447

Strand: Reverse

Name: oprF [H]

Synonym: Tbd_2454

Alternate gene names: 74318472

Gene position: 2526447-2525239 (Counterclockwise)

Preceding gene: 74318473

Following gene: 74318470

Centisome position: 86.83

GC content: 71.38

Gene sequence:

>1209_bases
ATGAAACGAGCACAGGGGGCCTGCCTGCTTGCCGGCCTCGCGCTGCTGGGCGGCGGCCAGGCGGCCGACATGCCGGCGCA
GGAAGTCGGTATTTTGTTGGGTGCGGGCTGGGCGGACGATAGTCTCGTCGGCAGCGAGGACGACGCGGCGAACCCGCTGT
TCGGCCTGCGCTACAGCCAGCAGCTCGATCGCGATTTCCGGCTGTTCGGCGATTTCGTCTACGGTGCCTACGACAGCGAC
CGACCGGGGGTCGGCGACGCGAAACTCGCCACGCTGCGTGGCGGCGTCGAGTGGGCGTTCTCCCGCCAACGGCAATACGA
CTGGTTCCTCGCCGGCGGTCTCGGCCTGATCCGCGCCGAGACCGACGGCGACGTCGACTTCACGCGCCCCGCGGCGTCGT
TCGGCGTCGGCCAGAACTGGGCGTTCGGTTCGAACGACGCGCTGCGCTGGGAGCTGCGTGCCGACCGTTCCTTCGGCAAC
GATGCGCTGCCGGGTGCGGGGTTGACCCAGGTTCAGGTCCTCGTCGGCTATGCCTGGGGCCTCGGCGAGCCGTCCGACGC
CGATGGTGACGGCGTGATCGACCGCGTCGAACAATGCCCCGGCACGCCCAAGGGTGCCGCGGTCGATGCGCTGGGCTGTC
CGCTCGATTCCGATGCCGACGGGGTGTTCGACGGCCTTGACCGCTGTCCGTCGACCCCGCCCGGCGCCACCGTCGACGCG
GCCGGCTGTCCCTCCGACCGCGACGGTGACGGCGTTCCCGACGCGCGCGACAAATGCCCGGCCGAAGCCGGGCGCGGCAG
CGCCGACGGCTGCCCGCCGCGGGCGGCGCGTGAAGCCGAACCCGAGCCCGAGCCGCCAAGGCCGTCCATGCCACGGCGGA
TGACGCTCGACGGCGTGAGCTTTGAGCCCGGCAGCGTGCGTCTGACGCCCGACTCGATCACGATCCTCGACAATGCCGCG
GCCTCGCTGAACGCATGGGGTGAAGTCAGGGTCGAGGTCGCCGGTCACACCGACTCGACCCACTCGGCCGCCTTCAACCT
CGAGCTCTCGCAGCGCCGGGCCGAGGCCGTGCGGGCCTATCTCGTGAAAAAAGGCGTTCGCGCCGAACGGCTGACCGCCC
GCGGCTACGGCGAGTCGCAGCCGGTTGCGGACAACCGCACCGCGGCCGGCCGCGCGCAGAACCGCCGCGTGGAACTCGTG
CCGCAGTAG

Upstream 100 bases:

>100_bases
AGTGCGGGCAAGTGGCCGGCGCAGCGTGCGCCGGGGCTACAATCGCCGCCGGCTGCTCGCTGTCGGCGAAGCAGGTTTTT
TGGTTGTTTGGGTGGATCAC

Downstream 100 bases:

>100_bases
CCACCTGGCTGCTAAAAAAAACGGGCGCTCGCGGCGCCCGTTTTTCATGGGCGAGACAGGACGCTCAGTGCGCCGCTTCC
CAGTTGCCGCCGACGCCGAC

Product: outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKRAQGACLLAGLALLGGGQAADMPAQEVGILLGAGWADDSLVGSEDDAANPLFGLRYSQQLDRDFRLFGDFVYGAYDSD
RPGVGDAKLATLRGGVEWAFSRQRQYDWFLAGGLGLIRAETDGDVDFTRPAASFGVGQNWAFGSNDALRWELRADRSFGN
DALPGAGLTQVQVLVGYAWGLGEPSDADGDGVIDRVEQCPGTPKGAAVDALGCPLDSDADGVFDGLDRCPSTPPGATVDA
AGCPSDRDGDGVPDARDKCPAEAGRGSADGCPPRAAREAEPEPEPPRPSMPRRMTLDGVSFEPGSVRLTPDSITILDNAA
ASLNAWGEVRVEVAGHTDSTHSAAFNLELSQRRAEAVRAYLVKKGVRAERLTARGYGESQPVADNRTAAGRAQNRRVELV
PQ

Sequences:

>Translated_402_residues
MKRAQGACLLAGLALLGGGQAADMPAQEVGILLGAGWADDSLVGSEDDAANPLFGLRYSQQLDRDFRLFGDFVYGAYDSD
RPGVGDAKLATLRGGVEWAFSRQRQYDWFLAGGLGLIRAETDGDVDFTRPAASFGVGQNWAFGSNDALRWELRADRSFGN
DALPGAGLTQVQVLVGYAWGLGEPSDADGDGVIDRVEQCPGTPKGAAVDALGCPLDSDADGVFDGLDRCPSTPPGATVDA
AGCPSDRDGDGVPDARDKCPAEAGRGSADGCPPRAAREAEPEPEPPRPSMPRRMTLDGVSFEPGSVRLTPDSITILDNAA
ASLNAWGEVRVEVAGHTDSTHSAAFNLELSQRRAEAVRAYLVKKGVRAERLTARGYGESQPVADNRTAAGRAQNRRVELV
PQ
>Mature_402_residues
MKRAQGACLLAGLALLGGGQAADMPAQEVGILLGAGWADDSLVGSEDDAANPLFGLRYSQQLDRDFRLFGDFVYGAYDSD
RPGVGDAKLATLRGGVEWAFSRQRQYDWFLAGGLGLIRAETDGDVDFTRPAASFGVGQNWAFGSNDALRWELRADRSFGN
DALPGAGLTQVQVLVGYAWGLGEPSDADGDGVIDRVEQCPGTPKGAAVDALGCPLDSDADGVFDGLDRCPSTPPGATVDA
AGCPSDRDGDGVPDARDKCPAEAGRGSADGCPPRAAREAEPEPEPPRPSMPRRMTLDGVSFEPGSVRLTPDSITILDNAA
ASLNAWGEVRVEVAGHTDSTHSAAFNLELSQRRAEAVRAYLVKKGVRAERLTARGYGESQPVADNRTAAGRAQNRRVELV
PQ

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=103, Percent_Identity=40.7766990291262, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788957, Length=105, Percent_Identity=35.2380952380952, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 42315; Mature: 42315

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAQGACLLAGLALLGGGQAADMPAQEVGILLGAGWADDSLVGSEDDAANPLFGLRYSQ
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHCEEEECCCCCCCCCCCCCCCCCCEECCHHHH
QLDRDFRLFGDFVYGAYDSDRPGVGDAKLATLRGGVEWAFSRQRQYDWFLAGGLGLIRAE
HHCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHCCHHHHHHCCCCCEEEEECCEEEEEEC
TDGDVDFTRPAASFGVGQNWAFGSNDALRWELRADRSFGNDALPGAGLTQVQVLVGYAWG
CCCCCEECCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHEEEEEHHHC
LGEPSDADGDGVIDRVEQCPGTPKGAAVDALGCPLDSDADGVFDGLDRCPSTPPGATVDA
CCCCCCCCCCHHHHHHHHCCCCCCCCEEHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEC
AGCPSDRDGDGVPDARDKCPAEAGRGSADGCPPRAAREAEPEPEPPRPSMPRRMTLDGVS
CCCCCCCCCCCCCCHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
FEPGSVRLTPDSITILDNAAASLNAWGEVRVEVAGHTDSTHSAAFNLELSQRRAEAVRAY
CCCCEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHH
LVKKGVRAERLTARGYGESQPVADNRTAAGRAQNRRVELVPQ
HHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MKRAQGACLLAGLALLGGGQAADMPAQEVGILLGAGWADDSLVGSEDDAANPLFGLRYSQ
CCCCCCHHHHHHHHHHCCCCCCCCCHHHHCEEEECCCCCCCCCCCCCCCCCCEECCHHHH
QLDRDFRLFGDFVYGAYDSDRPGVGDAKLATLRGGVEWAFSRQRQYDWFLAGGLGLIRAE
HHCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHCCHHHHHHCCCCCEEEEECCEEEEEEC
TDGDVDFTRPAASFGVGQNWAFGSNDALRWELRADRSFGNDALPGAGLTQVQVLVGYAWG
CCCCCEECCCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHEEEEEHHHC
LGEPSDADGDGVIDRVEQCPGTPKGAAVDALGCPLDSDADGVFDGLDRCPSTPPGATVDA
CCCCCCCCCCHHHHHHHHCCCCCCCCEEHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEC
AGCPSDRDGDGVPDARDKCPAEAGRGSADGCPPRAAREAEPEPEPPRPSMPRRMTLDGVS
CCCCCCCCCCCCCCHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
FEPGSVRLTPDSITILDNAAASLNAWGEVRVEVAGHTDSTHSAAFNLELSQRRAEAVRAY
CCCCEEEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHH
LVKKGVRAERLTARGYGESQPVADNRTAAGRAQNRRVELVPQ
HHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]