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The map label for this gene is mscL

Identifier: 74318473

GI number: 74318473

Start: 2526564

End: 2526989

Strand: Reverse

Name: mscL

Synonym: Tbd_2455

Alternate gene names: 74318473

Gene position: 2526989-2526564 (Counterclockwise)

Preceding gene: 74318485

Following gene: 74318472

Centisome position: 86.84

GC content: 63.62

Gene sequence:

>426_bases
ATGAGCTTCGCGTCCGAGTTCAAGCAATTCATCGCCAAGGGCAACGCAATGGACCTCGCGGTCGGCGTCATCATCGGCGC
CGCGTTCAGCAAGATCGTCGCGTCGATCGTCGACGACCTGATCATGCCGATCGTCGGCGCCGTGTTCGGCGGCTTCGATT
TTTCCAACCTCTTCATCGCGCTCGGCAGTGTGCCCGAGGGGGTGGCGTTGACGCTGGCGGAGGTCAGAAAGGCCGGCGTG
CCGGTCCTCGCCTACGGGAACTTCGTGACCGTGCTGCTCAATTTCCTGATCCTCGCCCTGATCGTCTTCATCATCGTCAG
GCAGATCAACCGCCTCAAGCGGCCGGCGCCCGGGGCCGCGCCGGCGGCGCCGCCCGAAGACATCGTGCTGCTGCGCGAGA
TTCGTGACGCGTTGAGACAAAAGTAG

Upstream 100 bases:

>100_bases
CCGGGACCCACCAAGCCGGCAAAAATTACCGCTGTACGCCCGGCCCGTTGGCCGGCAGGCTACTATGACCGGATCATCCA
GAAACCGGGAAAGGGGAGAT

Downstream 100 bases:

>100_bases
CGCCCTGAGCCGCGCAAGTGCGGGCAAGTGGCCGGCGCAGCGTGCGCCGGGGCTACAATCGCCGCCGGCTGCTCGCTGTC
GGCGAAGCAGGTTTTTTGGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MSFASEFKQFIAKGNAMDLAVGVIIGAAFSKIVASIVDDLIMPIVGAVFGGFDFSNLFIALGSVPEGVALTLAEVRKAGV
PVLAYGNFVTVLLNFLILALIVFIIVRQINRLKRPAPGAAPAAPPEDIVLLREIRDALRQK

Sequences:

>Translated_141_residues
MSFASEFKQFIAKGNAMDLAVGVIIGAAFSKIVASIVDDLIMPIVGAVFGGFDFSNLFIALGSVPEGVALTLAEVRKAGV
PVLAYGNFVTVLLNFLILALIVFIIVRQINRLKRPAPGAAPAAPPEDIVLLREIRDALRQK
>Mature_140_residues
SFASEFKQFIAKGNAMDLAVGVIIGAAFSKIVASIVDDLIMPIVGAVFGGFDFSNLFIALGSVPEGVALTLAEVRKAGVP
VLAYGNFVTVLLNFLILALIVFIIVRQINRLKRPAPGAAPAAPPEDIVLLREIRDALRQK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=145, Percent_Identity=44.1379310344828, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_THIDA (Q3SG48)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316213.1
- ProteinModelPortal:   Q3SG48
- SMR:   Q3SG48
- STRING:   Q3SG48
- GeneID:   3671774
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2455
- NMPDR:   fig|292415.3.peg.2528
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   Q3SG48
- BioCyc:   TDEN292415:TBD_2455-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14992; Mature: 14861

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e48a064)-; HASH(0x1da9309c)-; HASH(0x1b0196c8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFASEFKQFIAKGNAMDLAVGVIIGAAFSKIVASIVDDLIMPIVGAVFGGFDFSNLFIA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LGSVPEGVALTLAEVRKAGVPVLAYGNFVTVLLNFLILALIVFIIVRQINRLKRPAPGAA
HCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAAPPEDIVLLREIRDALRQK
CCCCHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SFASEFKQFIAKGNAMDLAVGVIIGAAFSKIVASIVDDLIMPIVGAVFGGFDFSNLFIA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LGSVPEGVALTLAEVRKAGVPVLAYGNFVTVLLNFLILALIVFIIVRQINRLKRPAPGAA
HCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAAPPEDIVLLREIRDALRQK
CCCCHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA