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The map label for this gene is metE

Identifier: 74318304

GI number: 74318304

Start: 2359679

End: 2361997

Strand: Direct

Name: metE

Synonym: Tbd_2286

Alternate gene names: 74318304

Gene position: 2359679-2361997 (Clockwise)

Preceding gene: 74318297

Following gene: 74318305

Centisome position: 81.09

GC content: 67.62

Gene sequence:

>2319_bases
ATGGCTCTCACCCACAACCTCGGCTTCCCGCGCATCGGCGCCCGGCGCGAACTCAAATTCGCGCTCGAGTCCTACTGGAA
GGGCGAATCCTCGCGCGACGCGCTCGAAGCGGTCGGCGCGGACCTGCGCCGCCGCCATTGGCAGGATCAGGCCGCGCTCG
ACCTCGTGCCGGTCGGCGACTTCGCCTTCTACGACCAGGTGCTCGACATGAGTTTCACGCTCGGCAACCTGCCCGAACGC
GTGCAGGGTTTCGACGGCCACCCGCTCGACAATGCCTTTCGCGTCGCCCGCGGCCGCTCGGCCGACATCGCCGAGACGCA
CTGCTGCAGCGCTGCGTGCAGCGGCGTGGCCGCCGGCGAGATGACCAAGTGGTTCGACACCAACTACCACTACATCGTCC
CCGAATTCGCGGCTTCCACGTCATTTAAGCTCGACGCGTCGCGCCTGCTCGCGCAACTGGCCGAAGCGCAAGCGGCGGGC
GTGCGCGCCAAGCCCGTGATCATCGGCCCGGTGACCTACCTTGCGCTCGGCAAGGCCAAGGACGGCTCCGACAAACTCGG
CCTGCTCGACCGCCTGCTGCCGGTCTACGTCGAACTGCTCGAGGCGCTGGCTGGCGCCGGGGCGGAATGGGTGCAGATCG
ACGAACCGCTGCTCGTCACCGAACTCAACGCCGAATGGCAACACGCCTTCAATCGCGCCTATCACGCGCTCAAGGCGAGC
CGGGTCAAGCTGCTGCTCGCGACCTATTTCGGCCAGCTCCTCGAAAACCGCTACCTCGCCGCCACCCTGCCCGTCGCCGG
TCTGCACGTCGACGCGATCAGCGGCCGCGACGACGTCGAGCCGCTCCTCGGCCTCTTGCCCGCGCACAAGGTCCTTTCGC
TCGGCGTCGTCAACGGCCGCAACATCTGGAAGACCGACCTCGGCGCCACGCTCGACTGGCTCGAACCCGTCGCCGAGCGC
CTCGGCGAGCGCCTGTGGATCGCGCCGTCGTGCTCGCTGCTGCATGTGCCGGTCGACCTCGACAGCGAAGATGCGTTGGA
CGGCGAGATCAAGTCCTGGCTCGCCTTCGCGAAGCAGAAGCTCGACGAACTGCGCGTGCTGGTCGCCGCGTTGAACCACG
GCCGCGACACGGTCAGCGCCGAGCTCGCTGCCAACCGCGCCGCGTGCGACGCGCGCCGCAACTCGCCGCGCGTCAACGAC
CCGGCGGTCAAAGCCGCGGTCGCCGCGATCACGCCGCAATCCGGTGCGCGCCAGAGCCCCTACGCCGTGCGTGCCGCGAA
GCAGGCCGCCCACCTCAGGCTTCCCCGGTTCCCCACCACGACGATCGGCTCGTTTCCGCAAACCGCCGAAATCCGCCAGG
CACGCCGCCGCTTTAAGGCCGGCGAGATCGACGACGCCGCCTACCGCGCCGCGATGCAGGACGAGATCGCACACAGCGTG
CGCGCGCAGGAAGCACTGGGGCTCGACGTGCTCGTGCACGGCGAGGCCGAGCGCAACGACATGGTCGAATATTTCGGCGA
GCAGCTCGACGGCTACGCGTTCAGCCGCTTCGGCTGGGTGCAGTCCTACGGCTCACGCTGCGTCAAGCCGCCGATCCTGT
TCGGCGACGTGCGCCGCCCCCGCCCCATGACCGTTGAGTGGACCCGGTACGGCCAGTCGCTGACCGAGAAGCCGATGAAG
GGCATGCTGACCGGCCCGGTGACGCTGCTCAACTGGTCCTTCGTGCGCGACGACCAGCCGCGCGCGGAGTCGTGCAAACA
GCTCGCCCTCACGATCCGCGACGAAGTGGCCGATCTCGAAAAGGCCGGCGTGCGCGTGATCCAGATCGACGAGGCCGCGC
TGCGCGAAGGCCTGCCGCTGCGCCGGTCGCAATGGAAAACCTACCTCGGCTGGGCCGTCGAATCCTTCCGCATCGCCGCC
AACGGCGTCGCCGACGAAACCCAGATCCACACCCACATGTGCTACTCGGAATTCAACGACATCATCGAAGCGATCGCCGC
GATGGACGCCGACGTCATCACCATCGAGACCTCGCGCTCCGACATGGAACTGCTCGACGCCTTCGATCATTTCAGCTATC
CGAACGAAATCGGCCCCGGCGTCTACGACATCCACTCGCCCAACATCCCGACGCCGCAGCACATCGTCAACCTGATCAAA
AAGGCCGCCGAGCGCATCCCCGCGGAGCGCCTGTGGATCAACCCGGACTGCGGCCTCAAGACGCGGCAGTGGAACGAGGT
GATTCCGGCGTTGGGGAACATGGTTGCGGCGGCGAGGACATTGCGGGCGGGATGGCAGGAGACAGGACGCGCAGGCTAA

Upstream 100 bases:

>100_bases
CCATGAGTGTTATTCATCGATGGATGAGAACATACCATTTTTGTTCATGTACCGTTTTGCCTAGGATGCCTCCACGTTCC
ATCAAAAAAGGTATCCCGCC

Downstream 100 bases:

>100_bases
CCCCGCAAGGCCGGCCAGCGCCTTGCGGCCCCGCAGGCCACCGGCCTATGATGCGTGGGCTTGCGGATGCCCCAGGGAGG
CGCCCATGACAACGAATGAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 772; Mature: 771

Protein sequence:

>772_residues
MALTHNLGFPRIGARRELKFALESYWKGESSRDALEAVGADLRRRHWQDQAALDLVPVGDFAFYDQVLDMSFTLGNLPER
VQGFDGHPLDNAFRVARGRSADIAETHCCSAACSGVAAGEMTKWFDTNYHYIVPEFAASTSFKLDASRLLAQLAEAQAAG
VRAKPVIIGPVTYLALGKAKDGSDKLGLLDRLLPVYVELLEALAGAGAEWVQIDEPLLVTELNAEWQHAFNRAYHALKAS
RVKLLLATYFGQLLENRYLAATLPVAGLHVDAISGRDDVEPLLGLLPAHKVLSLGVVNGRNIWKTDLGATLDWLEPVAER
LGERLWIAPSCSLLHVPVDLDSEDALDGEIKSWLAFAKQKLDELRVLVAALNHGRDTVSAELAANRAACDARRNSPRVND
PAVKAAVAAITPQSGARQSPYAVRAAKQAAHLRLPRFPTTTIGSFPQTAEIRQARRRFKAGEIDDAAYRAAMQDEIAHSV
RAQEALGLDVLVHGEAERNDMVEYFGEQLDGYAFSRFGWVQSYGSRCVKPPILFGDVRRPRPMTVEWTRYGQSLTEKPMK
GMLTGPVTLLNWSFVRDDQPRAESCKQLALTIRDEVADLEKAGVRVIQIDEAALREGLPLRRSQWKTYLGWAVESFRIAA
NGVADETQIHTHMCYSEFNDIIEAIAAMDADVITIETSRSDMELLDAFDHFSYPNEIGPGVYDIHSPNIPTPQHIVNLIK
KAAERIPAERLWINPDCGLKTRQWNEVIPALGNMVAAARTLRAGWQETGRAG

Sequences:

>Translated_772_residues
MALTHNLGFPRIGARRELKFALESYWKGESSRDALEAVGADLRRRHWQDQAALDLVPVGDFAFYDQVLDMSFTLGNLPER
VQGFDGHPLDNAFRVARGRSADIAETHCCSAACSGVAAGEMTKWFDTNYHYIVPEFAASTSFKLDASRLLAQLAEAQAAG
VRAKPVIIGPVTYLALGKAKDGSDKLGLLDRLLPVYVELLEALAGAGAEWVQIDEPLLVTELNAEWQHAFNRAYHALKAS
RVKLLLATYFGQLLENRYLAATLPVAGLHVDAISGRDDVEPLLGLLPAHKVLSLGVVNGRNIWKTDLGATLDWLEPVAER
LGERLWIAPSCSLLHVPVDLDSEDALDGEIKSWLAFAKQKLDELRVLVAALNHGRDTVSAELAANRAACDARRNSPRVND
PAVKAAVAAITPQSGARQSPYAVRAAKQAAHLRLPRFPTTTIGSFPQTAEIRQARRRFKAGEIDDAAYRAAMQDEIAHSV
RAQEALGLDVLVHGEAERNDMVEYFGEQLDGYAFSRFGWVQSYGSRCVKPPILFGDVRRPRPMTVEWTRYGQSLTEKPMK
GMLTGPVTLLNWSFVRDDQPRAESCKQLALTIRDEVADLEKAGVRVIQIDEAALREGLPLRRSQWKTYLGWAVESFRIAA
NGVADETQIHTHMCYSEFNDIIEAIAAMDADVITIETSRSDMELLDAFDHFSYPNEIGPGVYDIHSPNIPTPQHIVNLIK
KAAERIPAERLWINPDCGLKTRQWNEVIPALGNMVAAARTLRAGWQETGRAG
>Mature_771_residues
ALTHNLGFPRIGARRELKFALESYWKGESSRDALEAVGADLRRRHWQDQAALDLVPVGDFAFYDQVLDMSFTLGNLPERV
QGFDGHPLDNAFRVARGRSADIAETHCCSAACSGVAAGEMTKWFDTNYHYIVPEFAASTSFKLDASRLLAQLAEAQAAGV
RAKPVIIGPVTYLALGKAKDGSDKLGLLDRLLPVYVELLEALAGAGAEWVQIDEPLLVTELNAEWQHAFNRAYHALKASR
VKLLLATYFGQLLENRYLAATLPVAGLHVDAISGRDDVEPLLGLLPAHKVLSLGVVNGRNIWKTDLGATLDWLEPVAERL
GERLWIAPSCSLLHVPVDLDSEDALDGEIKSWLAFAKQKLDELRVLVAALNHGRDTVSAELAANRAACDARRNSPRVNDP
AVKAAVAAITPQSGARQSPYAVRAAKQAAHLRLPRFPTTTIGSFPQTAEIRQARRRFKAGEIDDAAYRAAMQDEIAHSVR
AQEALGLDVLVHGEAERNDMVEYFGEQLDGYAFSRFGWVQSYGSRCVKPPILFGDVRRPRPMTVEWTRYGQSLTEKPMKG
MLTGPVTLLNWSFVRDDQPRAESCKQLALTIRDEVADLEKAGVRVIQIDEAALREGLPLRRSQWKTYLGWAVESFRIAAN
GVADETQIHTHMCYSEFNDIIEAIAAMDADVITIETSRSDMELLDAFDHFSYPNEIGPGVYDIHSPNIPTPQHIVNLIKK
AAERIPAERLWINPDCGLKTRQWNEVIPALGNMVAAARTLRAGWQETGRAG

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=767, Percent_Identity=58.4093872229465, Blast_Score=869, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=776, Percent_Identity=46.9072164948454, Blast_Score=699, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_THIDA (Q3SGL1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316044.1
- HSSP:   O50008
- ProteinModelPortal:   Q3SGL1
- SMR:   Q3SGL1
- STRING:   Q3SGL1
- GeneID:   3673414
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2286
- NMPDR:   fig|292415.3.peg.2307
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   Q3SGL1
- ProtClustDB:   PRK05222
- BioCyc:   TDEN292415:TBD_2286-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85286; Mature: 85155

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTHNLGFPRIGARRELKFALESYWKGESSRDALEAVGADLRRRHWQDQAALDLVPVGD
CCEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FAFYDQVLDMSFTLGNLPERVQGFDGHPLDNAFRVARGRSADIAETHCCSAACSGVAAGE
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
MTKWFDTNYHYIVPEFAASTSFKLDASRLLAQLAEAQAAGVRAKPVIIGPVTYLALGKAK
HHHHHCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHEECCCC
DGSDKLGLLDRLLPVYVELLEALAGAGAEWVQIDEPLLVTELNAEWQHAFNRAYHALKAS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHH
RVKLLLATYFGQLLENRYLAATLPVAGLHVDAISGRDDVEPLLGLLPAHKVLSLGVVNGR
HHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCC
NIWKTDLGATLDWLEPVAERLGERLWIAPSCSLLHVPVDLDSEDALDGEIKSWLAFAKQK
CEEHHHHCCHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
LDELRVLVAALNHGRDTVSAELAANRAACDARRNSPRVNDPAVKAAVAAITPQSGARQSP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
YAVRAAKQAAHLRLPRFPTTTIGSFPQTAEIRQARRRFKAGEIDDAAYRAAMQDEIAHSV
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RAQEALGLDVLVHGEAERNDMVEYFGEQLDGYAFSRFGWVQSYGSRCVKPPILFGDVRRP
HHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEECCCCCC
RPMTVEWTRYGQSLTEKPMKGMLTGPVTLLNWSFVRDDQPRAESCKQLALTIRDEVADLE
CCCEEEHHHHCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
KAGVRVIQIDEAALREGLPLRRSQWKTYLGWAVESFRIAANGVADETQIHTHMCYSEFND
HCCEEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
IIEAIAAMDADVITIETSRSDMELLDAFDHFSYPNEIGPGVYDIHSPNIPTPQHIVNLIK
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHH
KAAERIPAERLWINPDCGLKTRQWNEVIPALGNMVAAARTLRAGWQETGRAG
HHHHHCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALTHNLGFPRIGARRELKFALESYWKGESSRDALEAVGADLRRRHWQDQAALDLVPVGD
CEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FAFYDQVLDMSFTLGNLPERVQGFDGHPLDNAFRVARGRSADIAETHCCSAACSGVAAGE
HHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
MTKWFDTNYHYIVPEFAASTSFKLDASRLLAQLAEAQAAGVRAKPVIIGPVTYLALGKAK
HHHHHCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHEECCCC
DGSDKLGLLDRLLPVYVELLEALAGAGAEWVQIDEPLLVTELNAEWQHAFNRAYHALKAS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHH
RVKLLLATYFGQLLENRYLAATLPVAGLHVDAISGRDDVEPLLGLLPAHKVLSLGVVNGR
HHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCC
NIWKTDLGATLDWLEPVAERLGERLWIAPSCSLLHVPVDLDSEDALDGEIKSWLAFAKQK
CEEHHHHCCHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHH
LDELRVLVAALNHGRDTVSAELAANRAACDARRNSPRVNDPAVKAAVAAITPQSGARQSP
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
YAVRAAKQAAHLRLPRFPTTTIGSFPQTAEIRQARRRFKAGEIDDAAYRAAMQDEIAHSV
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RAQEALGLDVLVHGEAERNDMVEYFGEQLDGYAFSRFGWVQSYGSRCVKPPILFGDVRRP
HHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEECCCCCC
RPMTVEWTRYGQSLTEKPMKGMLTGPVTLLNWSFVRDDQPRAESCKQLALTIRDEVADLE
CCCEEEHHHHCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
KAGVRVIQIDEAALREGLPLRRSQWKTYLGWAVESFRIAANGVADETQIHTHMCYSEFND
HCCEEEEEECHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
IIEAIAAMDADVITIETSRSDMELLDAFDHFSYPNEIGPGVYDIHSPNIPTPQHIVNLIK
HHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHH
KAAERIPAERLWINPDCGLKTRQWNEVIPALGNMVAAARTLRAGWQETGRAG
HHHHHCCHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA