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The map label for this gene is metR [H]

Identifier: 74318303

GI number: 74318303

Start: 2358643

End: 2359572

Strand: Reverse

Name: metR [H]

Synonym: Tbd_2285

Alternate gene names: 74318303

Gene position: 2359572-2358643 (Counterclockwise)

Preceding gene: 74318306

Following gene: 74318302

Centisome position: 81.09

GC content: 69.03

Gene sequence:

>930_bases
ATGGCCGCTCTCGAACGCATCCATCTCGAAATCGTCCGCGCGGTCGACCAGCACGGCTCGCTGACCGCCGCCGCCGAAAC
GCTCCACCTCACCCAATCGGCCTTGAGCCACGCGGTGCGCAAACTCGAGGATCAGCTCGGGCTCGCGGTCTGGCACCGCG
AGGGACGCAGCCTGCGGTCGACACAAGCGGGCGCCTACCTGCTGTCCATAGCCAATCGGCTGCTGCCGCAACTCGAACGC
GCCGAGGCCCAACTCGACCAGTTCGCCCGTGGCGAGCGCGGCACGCTGCGCATCGGCATGGAATGCCATCCCTGCTACGC
GTGGCTGCTGAAGATTGTGTCGCCCTACCTGGCCGCGTGGCCTGCAGTCGACGTCGACGTGAAGCAGAAATTCCAGTTCG
GCGGCGTCGGCGCGCTGTTCGCTTACGAGATCGACCTGCTCGTCACGCCCGATCCCTTCGACGCGCCTGGCCTGCGCTTC
GAGCCGGTGTTCGACTACGAGCAGGTGCTCGTCGTCGGCCCCGGCCACCCGTTGCGCCGGGCCGCCTACGTGCGCCCGCA
GCAACTCGCCGACGAGACGCTCATCACCTATCCGGTGGAGACCGACCGGCTCGACATCTACACGCGCTTCCTCACGCCGG
CCGGCGTCAGCCCCCGGCGCCACAAGACCATCGAGACCACCGACATCATGCTGCAGATGGTCGCCAGCGGCCGCGGCGTC
GCGGCGCTGCCGCGCTGGCTCGTCGAAGCGCAGGCCGACAGCTTCGACGTCGCCGCGGTCAGGCTCGGTCGCCGCGGCAT
GGCCAAGCAGATCTTCGTGGGTTTGCGCGAGGCGGATGTGGAGATCGACTACCTGCGCGCATTCGTCGAGTTGGCGCGCG
GCCGCGCGACGCGTTCGGGGCGCGGAAAGGAGCGCGCGCGCGGCTCTTGA

Upstream 100 bases:

>100_bases
ATACCTTTTTTGATGGAACGTGGAGGCATCCTAGGCAAAACGGTACATGAACAAAAATGGTATGTTCTCATCCATCGATG
AATAACACTCATGGAACGTC

Downstream 100 bases:

>100_bases
GTTCTGCGCGAGCCAGCGCCGCTCGGGCGAAACCGCATCCGCCGCCAACCGGCCACAGGCGACTACCCCCGCCTCGCCGC
CGAAAAAATCCGGCGCGGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MAALERIHLEIVRAVDQHGSLTAAAETLHLTQSALSHAVRKLEDQLGLAVWHREGRSLRSTQAGAYLLSIANRLLPQLER
AEAQLDQFARGERGTLRIGMECHPCYAWLLKIVSPYLAAWPAVDVDVKQKFQFGGVGALFAYEIDLLVTPDPFDAPGLRF
EPVFDYEQVLVVGPGHPLRRAAYVRPQQLADETLITYPVETDRLDIYTRFLTPAGVSPRRHKTIETTDIMLQMVASGRGV
AALPRWLVEAQADSFDVAAVRLGRRGMAKQIFVGLREADVEIDYLRAFVELARGRATRSGRGKERARGS

Sequences:

>Translated_309_residues
MAALERIHLEIVRAVDQHGSLTAAAETLHLTQSALSHAVRKLEDQLGLAVWHREGRSLRSTQAGAYLLSIANRLLPQLER
AEAQLDQFARGERGTLRIGMECHPCYAWLLKIVSPYLAAWPAVDVDVKQKFQFGGVGALFAYEIDLLVTPDPFDAPGLRF
EPVFDYEQVLVVGPGHPLRRAAYVRPQQLADETLITYPVETDRLDIYTRFLTPAGVSPRRHKTIETTDIMLQMVASGRGV
AALPRWLVEAQADSFDVAAVRLGRRGMAKQIFVGLREADVEIDYLRAFVELARGRATRSGRGKERARGS
>Mature_308_residues
AALERIHLEIVRAVDQHGSLTAAAETLHLTQSALSHAVRKLEDQLGLAVWHREGRSLRSTQAGAYLLSIANRLLPQLERA
EAQLDQFARGERGTLRIGMECHPCYAWLLKIVSPYLAAWPAVDVDVKQKFQFGGVGALFAYEIDLLVTPDPFDAPGLRFE
PVFDYEQVLVVGPGHPLRRAAYVRPQQLADETLITYPVETDRLDIYTRFLTPAGVSPRRHKTIETTDIMLQMVASGRGVA
ALPRWLVEAQADSFDVAAVRLGRRGMAKQIFVGLREADVEIDYLRAFVELARGRATRSGRGKERARGS

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=294, Percent_Identity=39.7959183673469, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=29.2452830188679, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789173, Length=234, Percent_Identity=29.4871794871795, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=25.609756097561, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI2367136, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787589, Length=249, Percent_Identity=27.3092369477912, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=256, Percent_Identity=23.046875, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789204, Length=216, Percent_Identity=28.7037037037037, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789639, Length=323, Percent_Identity=24.4582043343653, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790399, Length=237, Percent_Identity=25.7383966244726, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788296, Length=293, Percent_Identity=25.938566552901, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787949, Length=93, Percent_Identity=37.6344086021505, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082024, Length=282, Percent_Identity=26.241134751773, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34406; Mature: 34275

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALERIHLEIVRAVDQHGSLTAAAETLHLTQSALSHAVRKLEDQLGLAVWHREGRSLRS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TQAGAYLLSIANRLLPQLERAEAQLDQFARGERGTLRIGMECHPCYAWLLKIVSPYLAAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHC
PAVDVDVKQKFQFGGVGALFAYEIDLLVTPDPFDAPGLRFEPVFDYEQVLVVGPGHPLRR
CCCCCCHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCEECCCCCCCEEEEECCCCHHHH
AAYVRPQQLADETLITYPVETDRLDIYTRFLTPAGVSPRRHKTIETTDIMLQMVASGRGV
HHHCCHHHHCCCCEEEECCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
AALPRWLVEAQADSFDVAAVRLGRRGMAKQIFVGLREADVEIDYLRAFVELARGRATRSG
HHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
RGKERARGS
CCCCCCCCC
>Mature Secondary Structure 
AALERIHLEIVRAVDQHGSLTAAAETLHLTQSALSHAVRKLEDQLGLAVWHREGRSLRS
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
TQAGAYLLSIANRLLPQLERAEAQLDQFARGERGTLRIGMECHPCYAWLLKIVSPYLAAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHC
PAVDVDVKQKFQFGGVGALFAYEIDLLVTPDPFDAPGLRFEPVFDYEQVLVVGPGHPLRR
CCCCCCHHHHHCCCCEEEEEEEEEEEEECCCCCCCCCCEECCCCCCCEEEEECCCCHHHH
AAYVRPQQLADETLITYPVETDRLDIYTRFLTPAGVSPRRHKTIETTDIMLQMVASGRGV
HHHCCHHHHCCCCEEEECCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
AALPRWLVEAQADSFDVAAVRLGRRGMAKQIFVGLREADVEIDYLRAFVELARGRATRSG
HHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
RGKERARGS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]