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The map label for this gene is pcnB [H]

Identifier: 74318068

GI number: 74318068

Start: 2138010

End: 2139398

Strand: Direct

Name: pcnB [H]

Synonym: Tbd_2050

Alternate gene names: 74318068

Gene position: 2138010-2139398 (Clockwise)

Preceding gene: 74318067

Following gene: 74318069

Centisome position: 73.48

GC content: 69.19

Gene sequence:

>1389_bases
ATGATCCGCCGCATCATCAACAAAGTATTTGGCCGCACCTCGCGCGCCTCACGCAGCGAGGCGAAAATCCTGCCTCTGAA
AATGCACGGCATCCGGCGCGAACAGCTCGACGATTGCGCCCTCAAGGTGTGCGAAACCCTGGCCCAGGCGGGGTTCAAAG
GCTACCTGGTCGGCGGCGCGGTGCGCGATCTCCTGCTCGGCGGCACGCCGAAGGACTTCGACGTCGCGACCGACGCGACG
CCGGAAGAGGTGCGCCGGCTGTTCCGCCGCTCGCGCATCATCGGCCGCCGCTTCCAGATCGTGCACGTCATGTGCGGGCG
CGAGACGATCGAAGTCACGACCTTCCGCGCCAACGGCCAGAAGGAGGCCGAAGACGAAGACGCGCACGGCGACGATGCGC
CGGCTGCGTCGAGGCGCCTGACCGACGAGCACGGGCGCCTGCTGTCGGACAACGTGTTCGGCAGCATGGCGGACGACGCG
GCGCGCCGCGACTTCACGATCAACGCGCTCTACTACGACCCCGCGCGCGAAGAGGTGCACGACTATTTCGGCGGCGTCGG
CGACTGCAAGAAGCGGGTGCTGCGCATGATCGGGGATCCCGAGACGCGCTACCGCGAAGACCCGGTTCGCATGCTGCGCG
CCGCACGCTTTGCGGCCAAGCTCGACTTCCACATCGATCCCGAGACGCGCAAGCCGGTCGCCACGCTGGCCCCCCTGCTC
GCCAACATCCCGCGTGCGCGCATCTTCGACGAGGCGCTCAAACTGTTGCTCTCGGGCCACGCGCTGCGCGGCGTCCACCA
GTTACGCGCCGAGGGGCTGCACCACGGCATGCTGCCGCTGCTCGACACCATACTCGACGACACGACCGGCGAGCGTTTCA
TCACCGCGGCGCTGAAGAGCACCGACGCGCGCATCCAGTCCGACCGCCCGTCGTCGCCGGCCTTCCTGTTCGGGACCCTG
TTGTGGCCCCAGGTCGTCAAGCGCTGGAAAGCGCTCGAAGCGGCCGGCGAAAAACCGCAGCCGGCCTTGTTCGTCGCGAT
GGACGAGGTGCTCGACGCGCAGCGCGGCCAGCTCGCCATCCCGCGCCGCTACGACGGCATGATGAAGGAAATCTGGGCGC
TGCAGCCGCGCTTCGAGCAGCGCGGCGGGCAGCGGCCGTTCCGCCTGCTCGAACATCCGCGCTTCCGCGCCGCCTACGAC
TTCCTGCTGCTGCGCGCCGAAGCGGGCGAGGTCCCGAGCGAGCTCGGGGAATGGTGGACGCGCTTCCAGGAGGTCGACGA
CGACGCACGCGCCGGAATGCTCCTCGCCGACACGGCCGCCAAGCCGCGGCGGCGCCGCAAGCGCAGGAAGCCCGGCGAGG
AAGGCGCGCGCGAGACGCAACCGGCATGA

Upstream 100 bases:

>100_bases
CGGTGCCTGAACGCGCGGGCGCGCCATCGCCCGCGCCGGCCGCACCGCACCCAGCCTGTATAATCGGCCCTCTGCTCGAC
TCCGCTGCTTCGCGCCCTCA

Downstream 100 bases:

>100_bases
AGGTCGTCGCCACCATCGGCCTCGGCGCCAACCTCAACGACCCGGCGGCACAGGTCGAATATGCGCTGGCCGAGCTCGAC
CGCCTGCCCGCGACGCGCCT

Product: poly(A) polymerase, PcnB

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MIRRIINKVFGRTSRASRSEAKILPLKMHGIRREQLDDCALKVCETLAQAGFKGYLVGGAVRDLLLGGTPKDFDVATDAT
PEEVRRLFRRSRIIGRRFQIVHVMCGRETIEVTTFRANGQKEAEDEDAHGDDAPAASRRLTDEHGRLLSDNVFGSMADDA
ARRDFTINALYYDPAREEVHDYFGGVGDCKKRVLRMIGDPETRYREDPVRMLRAARFAAKLDFHIDPETRKPVATLAPLL
ANIPRARIFDEALKLLLSGHALRGVHQLRAEGLHHGMLPLLDTILDDTTGERFITAALKSTDARIQSDRPSSPAFLFGTL
LWPQVVKRWKALEAAGEKPQPALFVAMDEVLDAQRGQLAIPRRYDGMMKEIWALQPRFEQRGGQRPFRLLEHPRFRAAYD
FLLLRAEAGEVPSELGEWWTRFQEVDDDARAGMLLADTAAKPRRRRKRRKPGEEGARETQPA

Sequences:

>Translated_462_residues
MIRRIINKVFGRTSRASRSEAKILPLKMHGIRREQLDDCALKVCETLAQAGFKGYLVGGAVRDLLLGGTPKDFDVATDAT
PEEVRRLFRRSRIIGRRFQIVHVMCGRETIEVTTFRANGQKEAEDEDAHGDDAPAASRRLTDEHGRLLSDNVFGSMADDA
ARRDFTINALYYDPAREEVHDYFGGVGDCKKRVLRMIGDPETRYREDPVRMLRAARFAAKLDFHIDPETRKPVATLAPLL
ANIPRARIFDEALKLLLSGHALRGVHQLRAEGLHHGMLPLLDTILDDTTGERFITAALKSTDARIQSDRPSSPAFLFGTL
LWPQVVKRWKALEAAGEKPQPALFVAMDEVLDAQRGQLAIPRRYDGMMKEIWALQPRFEQRGGQRPFRLLEHPRFRAAYD
FLLLRAEAGEVPSELGEWWTRFQEVDDDARAGMLLADTAAKPRRRRKRRKPGEEGARETQPA
>Mature_462_residues
MIRRIINKVFGRTSRASRSEAKILPLKMHGIRREQLDDCALKVCETLAQAGFKGYLVGGAVRDLLLGGTPKDFDVATDAT
PEEVRRLFRRSRIIGRRFQIVHVMCGRETIEVTTFRANGQKEAEDEDAHGDDAPAASRRLTDEHGRLLSDNVFGSMADDA
ARRDFTINALYYDPAREEVHDYFGGVGDCKKRVLRMIGDPETRYREDPVRMLRAARFAAKLDFHIDPETRKPVATLAPLL
ANIPRARIFDEALKLLLSGHALRGVHQLRAEGLHHGMLPLLDTILDDTTGERFITAALKSTDARIQSDRPSSPAFLFGTL
LWPQVVKRWKALEAAGEKPQPALFVAMDEVLDAQRGQLAIPRRYDGMMKEIWALQPRFEQRGGQRPFRLLEHPRFRAAYD
FLLLRAEAGEVPSELGEWWTRFQEVDDDARAGMLLADTAAKPRRRRKRRKPGEEGARETQPA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=277, Percent_Identity=30.3249097472924, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI87081691, Length=429, Percent_Identity=47.3193473193473, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6321016, Length=181, Percent_Identity=30.3867403314917, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360112, Length=226, Percent_Identity=32.3008849557522, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357337, Length=226, Percent_Identity=32.3008849557522, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 52320; Mature: 52320

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRIINKVFGRTSRASRSEAKILPLKMHGIRREQLDDCALKVCETLAQAGFKGYLVGGA
CHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHH
VRDLLLGGTPKDFDVATDATPEEVRRLFRRSRIIGRRFQIVHVMCGRETIEVTTFRANGQ
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCC
KEAEDEDAHGDDAPAASRRLTDEHGRLLSDNVFGSMADDAARRDFTINALYYDPAREEVH
CCCCCCCCCCCCCCHHHHCCCHHHCCCHHHHHCCCHHHHHHHCCEEEEEEEECCHHHHHH
DYFGGVGDCKKRVLRMIGDPETRYREDPVRMLRAARFAAKLDFHIDPETRKPVATLAPLL
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHH
ANIPRARIFDEALKLLLSGHALRGVHQLRAEGLHHGMLPLLDTILDDTTGERFITAALKS
HCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHH
TDARIQSDRPSSPAFLFGTLLWPQVVKRWKALEAAGEKPQPALFVAMDEVLDAQRGQLAI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCEEC
PRRYDGMMKEIWALQPRFEQRGGQRPFRLLEHPRFRAAYDFLLLRAEAGEVPSELGEWWT
CHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
RFQEVDDDARAGMLLADTAAKPRRRRKRRKPGEEGARETQPA
HHHHCCCHHHCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRRIINKVFGRTSRASRSEAKILPLKMHGIRREQLDDCALKVCETLAQAGFKGYLVGGA
CHHHHHHHHHCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHH
VRDLLLGGTPKDFDVATDATPEEVRRLFRRSRIIGRRFQIVHVMCGRETIEVTTFRANGQ
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEECCCC
KEAEDEDAHGDDAPAASRRLTDEHGRLLSDNVFGSMADDAARRDFTINALYYDPAREEVH
CCCCCCCCCCCCCCHHHHCCCHHHCCCHHHHHCCCHHHHHHHCCEEEEEEEECCHHHHHH
DYFGGVGDCKKRVLRMIGDPETRYREDPVRMLRAARFAAKLDFHIDPETRKPVATLAPLL
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHH
ANIPRARIFDEALKLLLSGHALRGVHQLRAEGLHHGMLPLLDTILDDTTGERFITAALKS
HCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHH
TDARIQSDRPSSPAFLFGTLLWPQVVKRWKALEAAGEKPQPALFVAMDEVLDAQRGQLAI
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCEEC
PRRYDGMMKEIWALQPRFEQRGGQRPFRLLEHPRFRAAYDFLLLRAEAGEVPSELGEWWT
CHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHH
RFQEVDDDARAGMLLADTAAKPRRRRKRRKPGEEGARETQPA
HHHHCCCHHHCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]