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The map label for this gene is ppiB [H]

Identifier: 74318065

GI number: 74318065

Start: 2135609

End: 2136268

Strand: Direct

Name: ppiB [H]

Synonym: Tbd_2047

Alternate gene names: 74318065

Gene position: 2135609-2136268 (Clockwise)

Preceding gene: 74318064

Following gene: 74318066

Centisome position: 73.39

GC content: 67.27

Gene sequence:

>660_bases
ATGAACCGCCTGTCCCGCTTCTTCTCCCCCCTCTTCGTCGTCGCGCTGATGCTCGGCATGTCGGGCTGCGGCGCCAGCGA
GCCCGGCAAGCAGAACGTCTCGACCGCACCGGCGGCAGGGCAGCCCGCCAACCCGCGCGTGCTGATCGAAACCTCGAAAG
GCAACATCACGGTCGAACTCTTCGCCGCCAACGCCCCGCGGTCGACCGCCAATTTCCTCGACTACGTCGGGCAGGGTTTC
TACGACGGCACGATCTTCCACCGCGTGATTCCGGGTTTCATGATCCAGGGCGGCGGCATGACGGCGGACATGAATGAAAA
ACCCAACGGCGAGCCGATCCCGAACGAGGCCGACAACGGCCTCAAGAACCTGCGCGGGACGCTTGCGATGGCGCGCACCG
GCGACCCACACTCGGCGACCTCGCAGTTCTTCATCAACGTCGTCGACAACGCGCTGCTCAACCACCGCGGCAAGAGCGTC
CAGGGCTGGGGGTATGCGGTCTTCGGCCAGGTCGTCGACGGCATGGACGTCGTCGACGCGATCGTCGCGGTGCCACGCGG
CCCCTGGGGACCGCACGACGACGTCCCGGTCGAGCCGGTCGTGATGCGGCGCGTGAGCCTGTTGCCGCCAGCCGCGTCGG
CAGCAGCCGGCAAGCCATAA

Upstream 100 bases:

>100_bases
TCTCAGCGCATGAGCCGCGGACTGCGCGATTTCCCATCCCTTTTTGTTCGCCCCCGCTCACCCGTAGAATGCACGCGTTC
CCCTCGCCCGGAGTGTTCCG

Downstream 100 bases:

>100_bases
GACCCGCGCTGTCGGCCTCGGCGAGGCTGCTGCTGCTCGGTTTCGCGGCGTTCGCCGCGCTCGCGCTCGGCCTGACCGCC
GGCGCGCTGCCCGACGACAG

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase B; Rotamase B [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNRLSRFFSPLFVVALMLGMSGCGASEPGKQNVSTAPAAGQPANPRVLIETSKGNITVELFAANAPRSTANFLDYVGQGF
YDGTIFHRVIPGFMIQGGGMTADMNEKPNGEPIPNEADNGLKNLRGTLAMARTGDPHSATSQFFINVVDNALLNHRGKSV
QGWGYAVFGQVVDGMDVVDAIVAVPRGPWGPHDDVPVEPVVMRRVSLLPPAASAAAGKP

Sequences:

>Translated_219_residues
MNRLSRFFSPLFVVALMLGMSGCGASEPGKQNVSTAPAAGQPANPRVLIETSKGNITVELFAANAPRSTANFLDYVGQGF
YDGTIFHRVIPGFMIQGGGMTADMNEKPNGEPIPNEADNGLKNLRGTLAMARTGDPHSATSQFFINVVDNALLNHRGKSV
QGWGYAVFGQVVDGMDVVDAIVAVPRGPWGPHDDVPVEPVVMRRVSLLPPAASAAAGKP
>Mature_219_residues
MNRLSRFFSPLFVVALMLGMSGCGASEPGKQNVSTAPAAGQPANPRVLIETSKGNITVELFAANAPRSTANFLDYVGQGF
YDGTIFHRVIPGFMIQGGGMTADMNEKPNGEPIPNEADNGLKNLRGTLAMARTGDPHSATSQFFINVVDNALLNHRGKSV
QGWGYAVFGQVVDGMDVVDAIVAVPRGPWGPHDDVPVEPVVMRRVSLLPPAASAAAGKP

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI64276486, Length=134, Percent_Identity=37.3134328358209, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI19557636, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI24308049, Length=185, Percent_Identity=33.5135135135135, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI22547212, Length=157, Percent_Identity=35.031847133758, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI7657473, Length=157, Percent_Identity=35.031847133758, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI22547215, Length=157, Percent_Identity=35.031847133758, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI7706339, Length=165, Percent_Identity=34.5454545454545, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310110104, Length=150, Percent_Identity=35.3333333333333, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI310129267, Length=150, Percent_Identity=36.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310120620, Length=150, Percent_Identity=36.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI5454154, Length=193, Percent_Identity=30.5699481865285, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI10863927, Length=149, Percent_Identity=36.241610738255, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1786736, Length=161, Percent_Identity=60.2484472049689, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1789763, Length=166, Percent_Identity=59.0361445783133, Blast_Score=196, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI71980594, Length=162, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71980590, Length=142, Percent_Identity=35.9154929577465, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539496, Length=170, Percent_Identity=34.7058823529412, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17506311, Length=163, Percent_Identity=33.7423312883436, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71997782, Length=130, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17532641, Length=168, Percent_Identity=30.952380952381, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17552780, Length=176, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565860, Length=155, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17566266, Length=157, Percent_Identity=32.484076433121, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559074, Length=141, Percent_Identity=34.0425531914894, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=158, Percent_Identity=34.8101265822785, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321848, Length=173, Percent_Identity=34.6820809248555, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320359, Length=137, Percent_Identity=37.2262773722628, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323562, Length=156, Percent_Identity=33.974358974359, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922912, Length=179, Percent_Identity=33.5195530726257, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17986117, Length=157, Percent_Identity=35.031847133758, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24652460, Length=165, Percent_Identity=31.5151515151515, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922376, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549139, Length=183, Percent_Identity=33.879781420765, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357249, Length=165, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17136360, Length=166, Percent_Identity=33.7349397590361, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 23086; Mature: 23086

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLSRFFSPLFVVALMLGMSGCGASEPGKQNVSTAPAAGQPANPRVLIETSKGNITVEL
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEE
FAANAPRSTANFLDYVGQGFYDGTIFHRVIPGFMIQGGGMTADMNEKPNGEPIPNEADNG
EECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCEECCCCCCCCCCCCCHHHHH
LKNLRGTLAMARTGDPHSATSQFFINVVDNALLNHRGKSVQGWGYAVFGQVVDGMDVVDA
HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHH
IVAVPRGPWGPHDDVPVEPVVMRRVSLLPPAASAAAGKP
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNRLSRFFSPLFVVALMLGMSGCGASEPGKQNVSTAPAAGQPANPRVLIETSKGNITVEL
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEE
FAANAPRSTANFLDYVGQGFYDGTIFHRVIPGFMIQGGGMTADMNEKPNGEPIPNEADNG
EECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCEECCCCCCCCCCCCCHHHHH
LKNLRGTLAMARTGDPHSATSQFFINVVDNALLNHRGKSVQGWGYAVFGQVVDGMDVVDA
HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHH
IVAVPRGPWGPHDDVPVEPVVMRRVSLLPPAASAAAGKP
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2007139; 9278503; 1864365; 9298646; 9600841; 1606970; 8601841 [H]