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The map label for this gene is traI [H]

Identifier: 74317838

GI number: 74317838

Start: 1919351

End: 1922140

Strand: Reverse

Name: traI [H]

Synonym: Tbd_1820

Alternate gene names: 74317838

Gene position: 1922140-1919351 (Counterclockwise)

Preceding gene: 74317839

Following gene: 74317837

Centisome position: 66.06

GC content: 65.56

Gene sequence:

>2790_bases
ATGGTCTGCACAGTCAGCGCTATCAAAACCGCCGGCGTGGCGGCTGAGTACTACGCTCAGACAGACGACTATTATCGCGA
TCGGGGGCATGCCCCGACTGCATGGCAAGGCAAAGGCGCTGAGGCGCTGGGCCTGCGGGGCGAAGTCACCCCCGCGCAAG
CTGAGGCAATCCTGAACGGCATTCTGCCGAACGGTGAGCGGGTAGGCGGCGGCGACCACCGCCCCGGCTGGGATGCTACG
TTCTCAGCCCCGAAAAGTGTCAGCGTCGCGGCCTATGTACGCGGCGACGACCGCCTGATTGCAGCGCATGATCGAGCGGT
GAACGCTGCGATCCAATACCTGGAGCGAGAGGTGGCGGCCACCCGCATCCGCGAAGAAGGGGAGGTCCGCACGGAAGCGA
CGGGCAACCTGGTCGCCGCGACATATAGACATGACGTGTCGCGCGAGGGTGAGCCGGATCTACACACCCACAGCGTGATC
GCCAATATCACGCAGAGCGCAGACGGACAGTGGCGGTCGCTGGAGTCCCGCCCGTTGTACCGCCTGCAGACTGACGCGGG
AGCGGTGTATCGGGCGGCCCTTGCTCGTGAATGCGAGCGCCTGGGGTACGCGACCGAGCGCACCCGAGCCGGCGAGCATC
CTAGTTTTGAGTTGCGCCAGGTGTCACAGGCTGAGCGCGACATGTGGAGCACCAGGTCGCGGCAGGTGGAGGCCGAGCTG
GCAAAAATGGGGCTCTCGCGGGAGACCGCGACCGCCGAGCAAAAACAGGCCGCTGCGCTGGCGACGCGCCAGGCGAAGGG
CAACATAGGCCGCGATAACCTTTTATCCAATTGGCGAGAGCAGTCGCGGCAGGCCGGACACGAGATGCCAGCACTGCCGC
AGGCCCAGGAGATCGATGCGGGGGAGTATCGGCGTCGTGCCGAGGCGGCAGTACGGCAGGCGGTCGAGCATCTATCGGAG
CGTGAGAGCAGGTACACCGAGCGCCAGATCGTCACAGAAGCACGGAAGCTCGGGATGGGCGGCATCGACGACCAGGACAT
CCGGGCTGCCGTCGCCGACTTGCACCGGCGCGGCGAACTGGTGGAGACGCAGACCCGGCAGCACGACGCGATCACCGGCC
AGCGGCTGGAGCAGGCCGGCTACACGACGCGCGAGGCGCAGCGGATCGAGCGGCACATGCTCGACACGGCGAGCCGGGCC
GCTGGTGCAATCAAACCAGCGATGAGCATCGAGGCGGCCGACGCGGCGATTTCGGCGCAGGTGGCAAAAACCGGGCACGC
ATTTAACACCGCTCAGGTCGAGGCCACCAAGGCGCTGCTGACCGGCACGGATCGCATCACGCTGGTTCAAGGTTACGCGG
GCACAGCCAAGACCACCAGCGTGCTCGCCGTCTCATCTGCCGCGCTGCAACAGCAGGGTTATGGGGTCATCGCTTTGGCC
CCGACGCACTCGTCTGCGAAAACGTTGGGCGATGCGATTGGCGCCGAGTCGCAAACTGTCGCCAAATTCCTCAACAGCCG
ACCGGAGGTTAGCAGCCAGCCACGCATATATATGGTGGACGAGGCATCGATGCTGTCCGCCCGCGACATGGACAAGCTGT
TATCCCGCACCCAAGGGGGCCGGGTTGTCATGGTCGGCGACGTGAAACAGCTCGGCAGCGTCGAGGCGGGCGCTGCGTTT
CGTCAATTGCAAACGGACGGCGATCTCAAAACGCAGGTGCTCGATTATATCGTCCGCCAGCGTAGCGATGAGTTGCGGGC
GGCGGTCTACGACGCGATACGCGGCGACGTTCAGTCAGCGCTCAGCAAAATCGACGTACGCGAGCTGGACACGCGAGAGA
GCCGCGTCGGTGCGATCGCCGCCGACTATGCCGGCATGAGCCGCGACGACCGCGATCGCACAATCATCATTGCCCCCGGC
AGGGACGACCGGCGGCAGATCAACGATGCCGTGCGTGAGCAGTTGGCGGCGCGGGGCGAGTTGGGTGGCTCGGTGACGAT
CCAGGCGCTCGACTCGCGTGACATGACAGCGGTAGAGAGTCGGCGGGCCGGCAGCTATGCGGTTGGCCACCAGGTGCAGG
CCGGGCGGGACTATGCGTCGCTCGGCCTCAAACGCGGCGATTTTGCTCGCGTGGTTGCGGTGGACGTCGATTGCAACCGC
ATCACGCTCGAAACCGTCACCGGACACCAGAAAGAGATTGACCCGTCACGGGTGACGAAGCTCGCCGCGTTTGAGCAGCG
CGACATGGCTGTGGCGGTGGGCGACAAGCTGGTCAACCGTGTCAACACCCAGACGCTTAAAAACGGCACCCCGCTGACGG
TAGAGAAAATCGCCGAGGGCCAGATCCATGTCCGCGATGCCGCCGGCAAGCTGCACCGGCTCGACGCCGGAGCGATGCAC
CGGCTCGACCACGGCTACGCGCAGACTGGCCACGAATCCCAGGGCCGCACGTGTACGCGCGTACTAATACACGCGGAGAG
CGCGCGGACGAATCTGCAAACCCAGCAAAACATGTACGTCGCGCTCAGCCGCGCGACTGATGACGCACGGGTCTACACCG
ACAACCGCGGGGCCCTGGCTGCGCAGATCGAGCGTGAGTCGGGGCAGAAAGAGACCGCGCTGGCGGAGCCCGAGCCGCCC
CCGCCGGCCCTGGAGCTGCCGATGCCCGGCCCTGCGATCGAGCCAGCGCCGTGGGAGACGTCGGAGCCGGCGATCGATGT
CCGCACCCCCGACATATTAGAGATAGAGACGCCGCCACCCGCGGTGCCCGAGTTTGATTTTGATCGTTAG

Upstream 100 bases:

>100_bases
TCACTAGTGAGGCCGACCCCAGCACTGTCGAGGGCGACGGCAACACCACCGACACCACGGCTGATAGCGCCGACGTGCCG
TTTACTCTGGAGCGATAACC

Downstream 100 bases:

>100_bases
GAGACGACATGGACAAACTGATCCGACATTGGCCTGCCGCCCTGGCCGCCGCGCTTTTGATCGCGTTTGGTGCCGTGCAG
CTGGCGATTTTTAACACCGA

Product: TrwC protein

Products: NA

Alternate protein names: DNA helicase I [H]

Number of amino acids: Translated: 929; Mature: 929

Protein sequence:

>929_residues
MVCTVSAIKTAGVAAEYYAQTDDYYRDRGHAPTAWQGKGAEALGLRGEVTPAQAEAILNGILPNGERVGGGDHRPGWDAT
FSAPKSVSVAAYVRGDDRLIAAHDRAVNAAIQYLEREVAATRIREEGEVRTEATGNLVAATYRHDVSREGEPDLHTHSVI
ANITQSADGQWRSLESRPLYRLQTDAGAVYRAALARECERLGYATERTRAGEHPSFELRQVSQAERDMWSTRSRQVEAEL
AKMGLSRETATAEQKQAAALATRQAKGNIGRDNLLSNWREQSRQAGHEMPALPQAQEIDAGEYRRRAEAAVRQAVEHLSE
RESRYTERQIVTEARKLGMGGIDDQDIRAAVADLHRRGELVETQTRQHDAITGQRLEQAGYTTREAQRIERHMLDTASRA
AGAIKPAMSIEAADAAISAQVAKTGHAFNTAQVEATKALLTGTDRITLVQGYAGTAKTTSVLAVSSAALQQQGYGVIALA
PTHSSAKTLGDAIGAESQTVAKFLNSRPEVSSQPRIYMVDEASMLSARDMDKLLSRTQGGRVVMVGDVKQLGSVEAGAAF
RQLQTDGDLKTQVLDYIVRQRSDELRAAVYDAIRGDVQSALSKIDVRELDTRESRVGAIAADYAGMSRDDRDRTIIIAPG
RDDRRQINDAVREQLAARGELGGSVTIQALDSRDMTAVESRRAGSYAVGHQVQAGRDYASLGLKRGDFARVVAVDVDCNR
ITLETVTGHQKEIDPSRVTKLAAFEQRDMAVAVGDKLVNRVNTQTLKNGTPLTVEKIAEGQIHVRDAAGKLHRLDAGAMH
RLDHGYAQTGHESQGRTCTRVLIHAESARTNLQTQQNMYVALSRATDDARVYTDNRGALAAQIERESGQKETALAEPEPP
PPALELPMPGPAIEPAPWETSEPAIDVRTPDILEIETPPPAVPEFDFDR

Sequences:

>Translated_929_residues
MVCTVSAIKTAGVAAEYYAQTDDYYRDRGHAPTAWQGKGAEALGLRGEVTPAQAEAILNGILPNGERVGGGDHRPGWDAT
FSAPKSVSVAAYVRGDDRLIAAHDRAVNAAIQYLEREVAATRIREEGEVRTEATGNLVAATYRHDVSREGEPDLHTHSVI
ANITQSADGQWRSLESRPLYRLQTDAGAVYRAALARECERLGYATERTRAGEHPSFELRQVSQAERDMWSTRSRQVEAEL
AKMGLSRETATAEQKQAAALATRQAKGNIGRDNLLSNWREQSRQAGHEMPALPQAQEIDAGEYRRRAEAAVRQAVEHLSE
RESRYTERQIVTEARKLGMGGIDDQDIRAAVADLHRRGELVETQTRQHDAITGQRLEQAGYTTREAQRIERHMLDTASRA
AGAIKPAMSIEAADAAISAQVAKTGHAFNTAQVEATKALLTGTDRITLVQGYAGTAKTTSVLAVSSAALQQQGYGVIALA
PTHSSAKTLGDAIGAESQTVAKFLNSRPEVSSQPRIYMVDEASMLSARDMDKLLSRTQGGRVVMVGDVKQLGSVEAGAAF
RQLQTDGDLKTQVLDYIVRQRSDELRAAVYDAIRGDVQSALSKIDVRELDTRESRVGAIAADYAGMSRDDRDRTIIIAPG
RDDRRQINDAVREQLAARGELGGSVTIQALDSRDMTAVESRRAGSYAVGHQVQAGRDYASLGLKRGDFARVVAVDVDCNR
ITLETVTGHQKEIDPSRVTKLAAFEQRDMAVAVGDKLVNRVNTQTLKNGTPLTVEKIAEGQIHVRDAAGKLHRLDAGAMH
RLDHGYAQTGHESQGRTCTRVLIHAESARTNLQTQQNMYVALSRATDDARVYTDNRGALAAQIERESGQKETALAEPEPP
PPALELPMPGPAIEPAPWETSEPAIDVRTPDILEIETPPPAVPEFDFDR
>Mature_929_residues
MVCTVSAIKTAGVAAEYYAQTDDYYRDRGHAPTAWQGKGAEALGLRGEVTPAQAEAILNGILPNGERVGGGDHRPGWDAT
FSAPKSVSVAAYVRGDDRLIAAHDRAVNAAIQYLEREVAATRIREEGEVRTEATGNLVAATYRHDVSREGEPDLHTHSVI
ANITQSADGQWRSLESRPLYRLQTDAGAVYRAALARECERLGYATERTRAGEHPSFELRQVSQAERDMWSTRSRQVEAEL
AKMGLSRETATAEQKQAAALATRQAKGNIGRDNLLSNWREQSRQAGHEMPALPQAQEIDAGEYRRRAEAAVRQAVEHLSE
RESRYTERQIVTEARKLGMGGIDDQDIRAAVADLHRRGELVETQTRQHDAITGQRLEQAGYTTREAQRIERHMLDTASRA
AGAIKPAMSIEAADAAISAQVAKTGHAFNTAQVEATKALLTGTDRITLVQGYAGTAKTTSVLAVSSAALQQQGYGVIALA
PTHSSAKTLGDAIGAESQTVAKFLNSRPEVSSQPRIYMVDEASMLSARDMDKLLSRTQGGRVVMVGDVKQLGSVEAGAAF
RQLQTDGDLKTQVLDYIVRQRSDELRAAVYDAIRGDVQSALSKIDVRELDTRESRVGAIAADYAGMSRDDRDRTIIIAPG
RDDRRQINDAVREQLAARGELGGSVTIQALDSRDMTAVESRRAGSYAVGHQVQAGRDYASLGLKRGDFARVVAVDVDCNR
ITLETVTGHQKEIDPSRVTKLAAFEQRDMAVAVGDKLVNRVNTQTLKNGTPLTVEKIAEGQIHVRDAAGKLHRLDAGAMH
RLDHGYAQTGHESQGRTCTRVLIHAESARTNLQTQQNMYVALSRATDDARVYTDNRGALAAQIERESGQKETALAEPEPP
PPALELPMPGPAIEPAPWETSEPAIDVRTPDILEIETPPPAVPEFDFDR

Specific function: TraI has been identified as DNA helicase I and it also has an additional activity of site-specific nicking at oriT. DNA helicase I is a potent DNA-dependent ATPase [H]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traI of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014059
- InterPro:   IPR014129
- InterPro:   IPR009767
- InterPro:   IPR014862 [H]

Pfam domain/function: PF07057 TraI; PF08751 TrwC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 101144; Mature: 101144

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVCTVSAIKTAGVAAEYYAQTDDYYRDRGHAPTAWQGKGAEALGLRGEVTPAQAEAILNG
CEEECHHHHHHCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHH
ILPNGERVGGGDHRPGWDATFSAPKSVSVAAYVRGDDRLIAAHDRAVNAAIQYLEREVAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
TRIREEGEVRTEATGNLVAATYRHDVSREGEPDLHTHSVIANITQSADGQWRSLESRPLY
HHHHHCCCEEECCCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCE
RLQTDAGAVYRAALARECERLGYATERTRAGEHPSFELRQVSQAERDMWSTRSRQVEAEL
EEECCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKMGLSRETATAEQKQAAALATRQAKGNIGRDNLLSNWREQSRQAGHEMPALPQAQEIDA
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
GEYRRRAEAAVRQAVEHLSERESRYTERQIVTEARKLGMGGIDDQDIRAAVADLHRRGEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
VETQTRQHDAITGQRLEQAGYTTREAQRIERHMLDTASRAAGAIKPAMSIEAADAAISAQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAKTGHAFNTAQVEATKALLTGTDRITLVQGYAGTAKTTSVLAVSSAALQQQGYGVIALA
HHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
PTHSSAKTLGDAIGAESQTVAKFLNSRPEVSSQPRIYMVDEASMLSARDMDKLLSRTQGG
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
RVVMVGDVKQLGSVEAGAAFRQLQTDGDLKTQVLDYIVRQRSDELRAAVYDAIRGDVQSA
EEEEEECHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LSKIDVRELDTRESRVGAIAADYAGMSRDDRDRTIIIAPGRDDRRQINDAVREQLAARGE
HHHCCHHHHCCHHHHHCCHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
LGGSVTIQALDSRDMTAVESRRAGSYAVGHQVQAGRDYASLGLKRGDFARVVAVDVDCNR
CCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCE
ITLETVTGHQKEIDPSRVTKLAAFEQRDMAVAVGDKLVNRVNTQTLKNGTPLTVEKIAEG
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCC
QIHVRDAAGKLHRLDAGAMHRLDHGYAQTGHESQGRTCTRVLIHAESARTNLQTQQNMYV
EEEEEHHCCHHHHCCHHHHHHHHCCHHCCCCCCCCCEEEEEEEECHHHHCCCHHCCCEEE
ALSRATDDARVYTDNRGALAAQIERESGQKETALAEPEPPPPALELPMPGPAIEPAPWET
EEECCCCCCEEEECCCCCEEEEEEHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
SEPAIDVRTPDILEIETPPPAVPEFDFDR
CCCEEECCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MVCTVSAIKTAGVAAEYYAQTDDYYRDRGHAPTAWQGKGAEALGLRGEVTPAQAEAILNG
CEEECHHHHHHCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHH
ILPNGERVGGGDHRPGWDATFSAPKSVSVAAYVRGDDRLIAAHDRAVNAAIQYLEREVAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHH
TRIREEGEVRTEATGNLVAATYRHDVSREGEPDLHTHSVIANITQSADGQWRSLESRPLY
HHHHHCCCEEECCCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCE
RLQTDAGAVYRAALARECERLGYATERTRAGEHPSFELRQVSQAERDMWSTRSRQVEAEL
EEECCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKMGLSRETATAEQKQAAALATRQAKGNIGRDNLLSNWREQSRQAGHEMPALPQAQEIDA
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
GEYRRRAEAAVRQAVEHLSERESRYTERQIVTEARKLGMGGIDDQDIRAAVADLHRRGEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCH
VETQTRQHDAITGQRLEQAGYTTREAQRIERHMLDTASRAAGAIKPAMSIEAADAAISAQ
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VAKTGHAFNTAQVEATKALLTGTDRITLVQGYAGTAKTTSVLAVSSAALQQQGYGVIALA
HHHCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
PTHSSAKTLGDAIGAESQTVAKFLNSRPEVSSQPRIYMVDEASMLSARDMDKLLSRTQGG
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
RVVMVGDVKQLGSVEAGAAFRQLQTDGDLKTQVLDYIVRQRSDELRAAVYDAIRGDVQSA
EEEEEECHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LSKIDVRELDTRESRVGAIAADYAGMSRDDRDRTIIIAPGRDDRRQINDAVREQLAARGE
HHHCCHHHHCCHHHHHCCHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
LGGSVTIQALDSRDMTAVESRRAGSYAVGHQVQAGRDYASLGLKRGDFARVVAVDVDCNR
CCCCEEEEEECCCCCHHHHHHCCCCEECCCCCCCCCCHHHHCCCCCCCEEEEEEECCCCE
ITLETVTGHQKEIDPSRVTKLAAFEQRDMAVAVGDKLVNRVNTQTLKNGTPLTVEKIAEG
EEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCC
QIHVRDAAGKLHRLDAGAMHRLDHGYAQTGHESQGRTCTRVLIHAESARTNLQTQQNMYV
EEEEEHHCCHHHHCCHHHHHHHHCCHHCCCCCCCCCEEEEEEEECHHHHCCCHHCCCEEE
ALSRATDDARVYTDNRGALAAQIERESGQKETALAEPEPPPPALELPMPGPAIEPAPWET
EEECCCCCCEEEECCCCCEEEEEEHHCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
SEPAIDVRTPDILEIETPPPAVPEFDFDR
CCCEEECCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2163400; 7915817; 2680768; 2164585; 8736534 [H]