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The map label for this gene is vsr [H]

Identifier: 74317830

GI number: 74317830

Start: 1914837

End: 1915250

Strand: Direct

Name: vsr [H]

Synonym: Tbd_1812

Alternate gene names: 74317830

Gene position: 1914837-1915250 (Clockwise)

Preceding gene: 74317829

Following gene: 74317834

Centisome position: 65.81

GC content: 61.35

Gene sequence:

>414_bases
ATGACCGAAACGCCGGAGCTGCGCAGCCGCACAATGCGAGCTGTGCGCTCCCGCGACACCACCCCCGAACTGGCCGTCCG
CCGGCTTCTGCGCGGCATGGGCCTCACAGGCTATCGTCTCCATCGCAATGGGCTACCCGGTAAGCCGGACGTCGTTTTCG
TTGGTCGAAAGAAGGCAATCTTCATTCACGGCTGTTTCTGGCACGGCCACGATTGTCAACGCGGGCGGCGAGTGCCGCTG
ACAAACGCAGACTACTGGTTGCCCAAGATTCAACGCAACCGGCAGCGCGACGCCAAGCACTTGACCGAGTTGGCACGGCT
TGGCTGGTCCGTACTAACGGTCTGGGAGTGCGATCTGCGCGACCCTATTGAACTAACCGCAAGACTCCGTTCATTCCTTG
AGCGCACCGTCTAG

Upstream 100 bases:

>100_bases
CTGACGGCCGCCGCGTCGCGGTCGGCGATCTGGTGACGCGCTACAACGAAATCATTGAAGAAGTCGAAACCGATCCAAGC
CTCAAGATCGAGTTTCGCCA

Downstream 100 bases:

>100_bases
CCACTTCGCGCCTACGGCCTCCAACTTTATCGAAGCATAACAAGAGGTATTCGTGATAACCCGCATAATCACGAATGGCA
GATTTCGAGTCTAATCGTCG

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MTETPELRSRTMRAVRSRDTTPELAVRRLLRGMGLTGYRLHRNGLPGKPDVVFVGRKKAIFIHGCFWHGHDCQRGRRVPL
TNADYWLPKIQRNRQRDAKHLTELARLGWSVLTVWECDLRDPIELTARLRSFLERTV

Sequences:

>Translated_137_residues
MTETPELRSRTMRAVRSRDTTPELAVRRLLRGMGLTGYRLHRNGLPGKPDVVFVGRKKAIFIHGCFWHGHDCQRGRRVPL
TNADYWLPKIQRNRQRDAKHLTELARLGWSVLTVWECDLRDPIELTARLRSFLERTV
>Mature_136_residues
TETPELRSRTMRAVRSRDTTPELAVRRLLRGMGLTGYRLHRNGLPGKPDVVFVGRKKAIFIHGCFWHGHDCQRGRRVPLT
NADYWLPKIQRNRQRDAKHLTELARLGWSVLTVWECDLRDPIELTARLRSFLERTV

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=119, Percent_Identity=46.218487394958, Blast_Score=106, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16057; Mature: 15926

Theoretical pI: Translated: 11.64; Mature: 11.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETPELRSRTMRAVRSRDTTPELAVRRLLRGMGLTGYRLHRNGLPGKPDVVFVGRKKAI
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCEEE
FIHGCFWHGHDCQRGRRVPLTNADYWLPKIQRNRQRDAKHLTELARLGWSVLTVWECDLR
EEEEEEECCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
DPIELTARLRSFLERTV
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TETPELRSRTMRAVRSRDTTPELAVRRLLRGMGLTGYRLHRNGLPGKPDVVFVGRKKAI
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCCEEE
FIHGCFWHGHDCQRGRRVPLTNADYWLPKIQRNRQRDAKHLTELARLGWSVLTVWECDLR
EEEEEEECCCCCCCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
DPIELTARLRSFLERTV
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]