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The map label for this gene is narK [H]

Identifier: 74317420

GI number: 74317420

Start: 1483072

End: 1484748

Strand: Direct

Name: narK [H]

Synonym: Tbd_1402

Alternate gene names: 74317420

Gene position: 1483072-1484748 (Clockwise)

Preceding gene: 74317419

Following gene: 74317421

Centisome position: 50.97

GC content: 64.04

Gene sequence:

>1677_bases
ATGAGTCTCAGAAACAAGCAGCACGGCGCCGCGGGCGTCGCCGTCGGGGGACGCGAGCGCGAGCAGGTTCCCGCGACCAT
TCCCGAGTGGGACGTCGAGAACAATGTGTTCTGGGAATCGACGGGCAAGCGCATCGCCAACCGCAACCTGTGGATTTCGA
TCCCGGCCCTGTTGTGCGGATTCGCGGTGTGGGGCATGTGGGGCATCATCACCGTACAGATGCTCAATCTCGGGTTCCCC
TTCTCCAAGGAGGAGCTCTTCACGCTCACCGCGATCGCCGGCATCTCGGGCGCCACGATGCGGATTCCGTCGTCGTTCTT
CATTCGTCTCGCGGGCGGGCGCAACGTCATTTTCCTGACCACGGCGATGCTGCTCGCGCCGGCGATCGGCACGGGCATCG
TGCTGCAACACCCCGACTGGCCCTTGTGGACCTTCCAGCTAATGGCGCTGTGGTCGGGCGTCGGCGGCGGCAACTTCGCC
AGCTCGATGTCGAACATCAGCACCTTCTTCCCCAAGCGTCTCCAGGGCACGGCGCTCGGCCTCAACGCGGGCCTGGGCAA
CTTCGGCGTAACCACGATGCAGGTCGTGATTCCGCTGGTCATGACTCTCGGCATGCTCGGCAGCTACGGCGGCGAATCGA
TGACGCTGGTCAAGGACAGCGGCTGGATCCTCGGCAAGATCGCCGCGGGCACCGAGACCTACGTGCAGAACGCCGGCTTC
GCCTGGGTGCTGTCGCTGGTGCCGCTGGCCATCCTGGCCTGGTTCGGGATGAACAACCTGAAGCCGATCTCGCCGGACGC
GCATCACACGATCGCGTCCTTCGCCAAGGTCACCTACCTCTACACGCTGGCCTTCCTGCCGGCGATCTTCATGCTCTACC
TGTATCTGCCGGCGCCGACCGGGCTCGGCGTACTCAACATGTGGGTCGCCATTCCGCTCGATATCCTCGCGGCGCTGCTG
GTGATGAAGCTTGCAGCGTTCGGCACCATGAAGGAAAACGTCGCCAAGCAGTTCGCAATCTTCAAGAACAAGCACACCTG
GTCCCTGACCGCGCTGTACATCGTCACCTTCGGCTCGTTCATCGGCTTCTCGATGGCGCTGCCGTTGTCGATCACGGTCA
TCTTCGGCGACTCGCACGTGCTCGATGCGGCAACCGGCGTCTGGACCCATAGCAAGAACCCCAACGCGCCCTCGGCCTTC
ACCTATGCGTGGATCGGCCCCTTCGTCGGCGCGGCGATGCGCCCGATCGGCGGCTGGATTTCGGACAAGGTCGGCGGATC
GATCGTCACCCAGATCATCTCCGCCGTGATGGTCGCCGCCTCGGCCTACGCGGGCTGGATCATGATGCAGGCCTTCCACT
CGGCCACGCCCGAAGTCTATTTCACCCAGTTCCTCGTTACCTTCATCGTGCTCTTTGCCGCCACCGGTGTTGGCAACGGC
TCGACCTTCCGCACCGTCGGCGTGATCTTCGACCGCGTGCAGGCGGGGCCGGTGCTCGGCTGGACGTCGGCGGTCGCCGC
CTACGGCGCGTTCATCGCCCCGGTGGTGATCGGCGATCAGATCAAGGCCGGCTCGCCGGAGAACGCGATGTACGGCTTCG
CCGCCTTCTATGCCGTGTGCCTGTTCGTGAACTGGTGGTTCTACCTGCGCAAGCACGCCTACATCAAGAACCCCTGA

Upstream 100 bases:

>100_bases
GTACGCGAGATGGACGCGGCGGCGCGGGGCGCGAAGCGCGTCATCGCCGCGGACGTCATGGAGTAACTGTGTCGATCAGC
CGAATGGAGGGCAAGGGATC

Downstream 100 bases:

>100_bases
TCGCAGCCTGAAACCCCAAGGAGCAAGAACAATGAGTCACTTTCTCGACCGACTGACCTACTTCACCCGCCAGCGCGAGT
CGTTTTCCGGCGATCACGGC

Product: nitrate/nitrite antiporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSLRNKQHGAAGVAVGGREREQVPATIPEWDVENNVFWESTGKRIANRNLWISIPALLCGFAVWGMWGIITVQMLNLGFP
FSKEELFTLTAIAGISGATMRIPSSFFIRLAGGRNVIFLTTAMLLAPAIGTGIVLQHPDWPLWTFQLMALWSGVGGGNFA
SSMSNISTFFPKRLQGTALGLNAGLGNFGVTTMQVVIPLVMTLGMLGSYGGESMTLVKDSGWILGKIAAGTETYVQNAGF
AWVLSLVPLAILAWFGMNNLKPISPDAHHTIASFAKVTYLYTLAFLPAIFMLYLYLPAPTGLGVLNMWVAIPLDILAALL
VMKLAAFGTMKENVAKQFAIFKNKHTWSLTALYIVTFGSFIGFSMALPLSITVIFGDSHVLDAATGVWTHSKNPNAPSAF
TYAWIGPFVGAAMRPIGGWISDKVGGSIVTQIISAVMVAASAYAGWIMMQAFHSATPEVYFTQFLVTFIVLFAATGVGNG
STFRTVGVIFDRVQAGPVLGWTSAVAAYGAFIAPVVIGDQIKAGSPENAMYGFAAFYAVCLFVNWWFYLRKHAYIKNP

Sequences:

>Translated_558_residues
MSLRNKQHGAAGVAVGGREREQVPATIPEWDVENNVFWESTGKRIANRNLWISIPALLCGFAVWGMWGIITVQMLNLGFP
FSKEELFTLTAIAGISGATMRIPSSFFIRLAGGRNVIFLTTAMLLAPAIGTGIVLQHPDWPLWTFQLMALWSGVGGGNFA
SSMSNISTFFPKRLQGTALGLNAGLGNFGVTTMQVVIPLVMTLGMLGSYGGESMTLVKDSGWILGKIAAGTETYVQNAGF
AWVLSLVPLAILAWFGMNNLKPISPDAHHTIASFAKVTYLYTLAFLPAIFMLYLYLPAPTGLGVLNMWVAIPLDILAALL
VMKLAAFGTMKENVAKQFAIFKNKHTWSLTALYIVTFGSFIGFSMALPLSITVIFGDSHVLDAATGVWTHSKNPNAPSAF
TYAWIGPFVGAAMRPIGGWISDKVGGSIVTQIISAVMVAASAYAGWIMMQAFHSATPEVYFTQFLVTFIVLFAATGVGNG
STFRTVGVIFDRVQAGPVLGWTSAVAAYGAFIAPVVIGDQIKAGSPENAMYGFAAFYAVCLFVNWWFYLRKHAYIKNP
>Mature_557_residues
SLRNKQHGAAGVAVGGREREQVPATIPEWDVENNVFWESTGKRIANRNLWISIPALLCGFAVWGMWGIITVQMLNLGFPF
SKEELFTLTAIAGISGATMRIPSSFFIRLAGGRNVIFLTTAMLLAPAIGTGIVLQHPDWPLWTFQLMALWSGVGGGNFAS
SMSNISTFFPKRLQGTALGLNAGLGNFGVTTMQVVIPLVMTLGMLGSYGGESMTLVKDSGWILGKIAAGTETYVQNAGFA
WVLSLVPLAILAWFGMNNLKPISPDAHHTIASFAKVTYLYTLAFLPAIFMLYLYLPAPTGLGVLNMWVAIPLDILAALLV
MKLAAFGTMKENVAKQFAIFKNKHTWSLTALYIVTFGSFIGFSMALPLSITVIFGDSHVLDAATGVWTHSKNPNAPSAFT
YAWIGPFVGAAMRPIGGWISDKVGGSIVTQIISAVMVAASAYAGWIMMQAFHSATPEVYFTQFLVTFIVLFAATGVGNGS
TFRTVGVIFDRVQAGPVLGWTSAVAAYGAFIAPVVIGDQIKAGSPENAMYGFAAFYAVCLFVNWWFYLRKHAYIKNP

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=236, Percent_Identity=43.6440677966102, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1787743, Length=232, Percent_Identity=43.1034482758621, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 60163; Mature: 60032

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRNKQHGAAGVAVGGREREQVPATIPEWDVENNVFWESTGKRIANRNLWISIPALLCG
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCCEEEEHHHHHHH
FAVWGMWGIITVQMLNLGFPFSKEELFTLTAIAGISGATMRIPSSFFIRLAGGRNVIFLT
HHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHHCCCCCEEECCCEEEEEECCCCEEEHHH
TAMLLAPAIGTGIVLQHPDWPLWTFQLMALWSGVGGGNFASSMSNISTFFPKRLQGTALG
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
LNAGLGNFGVTTMQVVIPLVMTLGMLGSYGGESMTLVKDSGWILGKIAAGTETYVQNAGF
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHCCCH
AWVLSLVPLAILAWFGMNNLKPISPDAHHTIASFAKVTYLYTLAFLPAIFMLYLYLPAPT
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLGVLNMWVAIPLDILAALLVMKLAAFGTMKENVAKQFAIFKNKHTWSLTALYIVTFGSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGFSMALPLSITVIFGDSHVLDAATGVWTHSKNPNAPSAFTYAWIGPFVGAAMRPIGGWI
HHHHEECCEEEEEEECCCCHHHHHCCCEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCHH
SDKVGGSIVTQIISAVMVAASAYAGWIMMQAFHSATPEVYFTQFLVTFIVLFAATGVGNG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
STFRTVGVIFDRVQAGPVLGWTSAVAAYGAFIAPVVIGDQIKAGSPENAMYGFAAFYAVC
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LFVNWWFYLRKHAYIKNP
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLRNKQHGAAGVAVGGREREQVPATIPEWDVENNVFWESTGKRIANRNLWISIPALLCG
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCCEEEEHHHHHHH
FAVWGMWGIITVQMLNLGFPFSKEELFTLTAIAGISGATMRIPSSFFIRLAGGRNVIFLT
HHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHHCCCCCEEECCCEEEEEECCCCEEEHHH
TAMLLAPAIGTGIVLQHPDWPLWTFQLMALWSGVGGGNFASSMSNISTFFPKRLQGTALG
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
LNAGLGNFGVTTMQVVIPLVMTLGMLGSYGGESMTLVKDSGWILGKIAAGTETYVQNAGF
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHCCCH
AWVLSLVPLAILAWFGMNNLKPISPDAHHTIASFAKVTYLYTLAFLPAIFMLYLYLPAPT
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLGVLNMWVAIPLDILAALLVMKLAAFGTMKENVAKQFAIFKNKHTWSLTALYIVTFGSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGFSMALPLSITVIFGDSHVLDAATGVWTHSKNPNAPSAFTYAWIGPFVGAAMRPIGGWI
HHHHEECCEEEEEEECCCCHHHHHCCCEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCHH
SDKVGGSIVTQIISAVMVAASAYAGWIMMQAFHSATPEVYFTQFLVTFIVLFAATGVGNG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
STFRTVGVIFDRVQAGPVLGWTSAVAAYGAFIAPVVIGDQIKAGSPENAMYGFAAFYAVC
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LFVNWWFYLRKHAYIKNP
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]