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The map label for this gene is narK [H]

Identifier: 74317419

GI number: 74317419

Start: 1481769

End: 1483037

Strand: Direct

Name: narK [H]

Synonym: Tbd_1401

Alternate gene names: 74317419

Gene position: 1481769-1483037 (Clockwise)

Preceding gene: 74317415

Following gene: 74317420

Centisome position: 50.92

GC content: 63.99

Gene sequence:

>1269_bases
ATGCAGAGCCAACAATACAAGGCATGGTCGGTCGTCAGCATGAGCACGCTGGCGTTCACGGTCTGCTTCATGATCTGGAT
GATGTTCGCCGTCATCGGCGTTCCCATCAAACAGCAACTCGGCCTCAACGAGACGCAGTTCGGCATCCTCGTCGCCACCC
CGGTCCTGACCGGGTCGCTGATCCGCGTGCCGCTCGGCATGTGGACGGACAAATTCGGCGGCCGCATCGTGTTCTTCGTC
CTGATGCTGACGACGGTCATCCCGATCTGGCTGATCTCCTACGCGACCGAGTTCTGGCAGTTTCTGCTGGTCGGCCTGTT
CGTCGGCCTGGCCGGCGGCTCGTTCACGGTCGGCATCGCCTACTGCGCGCGCTGGTTCCCGCGTGAGCGCCAGGGGCTGG
CGATGGGCATTTTCGGCGCGGGCAATACCGGCGCCGCCGTCACCAAGCTGCTCGCACCGATCATCGTCGTCGGATACGGC
TGGACGATGGTGCCCAAGGTCTACGCCGGACTCATGCTCGTCACCGCCATCGTCTTCTGGTTCTTCACCTACACCGAGCC
CGCCCACAAGGTGAGCAAGTCGGTCACGATCCGCGAGCAGCTTGCGGCCTTCAAGGATCCGAAGGTCTGGCGCTACAGCC
AGTACTACTCGATCGTGTTCGGCGGCTACGTGGCGCTCGCGCTGTGGATGACCAAGTACTACGTGAGCCAGTACGGCTTC
GATCTCAAGACCGCGGCCTTCCTGGCGGCCGCCTTCAGCATTCCCGGCGGCGTGCTGCGCGCGGTCGGCGGCTACTTCTC
CGACAAGTACGGCGCGCACACGATCACCTGGTGGGTGCTGTGGGTCTCGCTGCTGTGCCTGTTCTTCCTGTCCTACCCGC
AGAGCAGCATCACGATCCACACCGTCGACGGCCTGCGCACCTTCCACTTCGGCCTCGGCCCGACGGCGTTCACGATCGTG
ATGTTCAGCCTCGGCATCGCGTTCGCGATCGGCAAGGCCTCTGTCTTCAAGTACATCTCCGACGACTACCCCGACAACAT
CGGCGTGATCTCCGGCGTGGTCGGCCTCGCCGGCGGTCTCGGCGGCTTCCTGATGCCGATCATGTTCGGCGCCCTGGTCG
ACCTGACCGGCGTCCGCTCGTCGGCCTTCATGCTGATGTTCGGCGTCGTGTGGGTGTCGCTGATGTGGATGTATTTCACC
GAAGTACGCGAGATGGACGCGGCGGCGCGGGGCGCGAAGCGCGTCATCGCCGCGGACGTCATGGAGTAA

Upstream 100 bases:

>100_bases
CGCGCCCTTCCGACCGGCGCTTCCCCCTCTGGCCTGTGTGGGTTCAAGGGGGTAAAAAGACCCCTAAGTCCATTTTACCG
GTTCCGTCGCGGGGTGGTTT

Downstream 100 bases:

>100_bases
CTGTGTCGATCAGCCGAATGGAGGGCAAGGGATCATGAGTCTCAGAAACAAGCAGCACGGCGCCGCGGGCGTCGCCGTCG
GGGGACGCGAGCGCGAGCAG

Product: nitrate/proton symporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MQSQQYKAWSVVSMSTLAFTVCFMIWMMFAVIGVPIKQQLGLNETQFGILVATPVLTGSLIRVPLGMWTDKFGGRIVFFV
LMLTTVIPIWLISYATEFWQFLLVGLFVGLAGGSFTVGIAYCARWFPRERQGLAMGIFGAGNTGAAVTKLLAPIIVVGYG
WTMVPKVYAGLMLVTAIVFWFFTYTEPAHKVSKSVTIREQLAAFKDPKVWRYSQYYSIVFGGYVALALWMTKYYVSQYGF
DLKTAAFLAAAFSIPGGVLRAVGGYFSDKYGAHTITWWVLWVSLLCLFFLSYPQSSITIHTVDGLRTFHFGLGPTAFTIV
MFSLGIAFAIGKASVFKYISDDYPDNIGVISGVVGLAGGLGGFLMPIMFGALVDLTGVRSSAFMLMFGVVWVSLMWMYFT
EVREMDAAARGAKRVIAADVME

Sequences:

>Translated_422_residues
MQSQQYKAWSVVSMSTLAFTVCFMIWMMFAVIGVPIKQQLGLNETQFGILVATPVLTGSLIRVPLGMWTDKFGGRIVFFV
LMLTTVIPIWLISYATEFWQFLLVGLFVGLAGGSFTVGIAYCARWFPRERQGLAMGIFGAGNTGAAVTKLLAPIIVVGYG
WTMVPKVYAGLMLVTAIVFWFFTYTEPAHKVSKSVTIREQLAAFKDPKVWRYSQYYSIVFGGYVALALWMTKYYVSQYGF
DLKTAAFLAAAFSIPGGVLRAVGGYFSDKYGAHTITWWVLWVSLLCLFFLSYPQSSITIHTVDGLRTFHFGLGPTAFTIV
MFSLGIAFAIGKASVFKYISDDYPDNIGVISGVVGLAGGLGGFLMPIMFGALVDLTGVRSSAFMLMFGVVWVSLMWMYFT
EVREMDAAARGAKRVIAADVME
>Mature_422_residues
MQSQQYKAWSVVSMSTLAFTVCFMIWMMFAVIGVPIKQQLGLNETQFGILVATPVLTGSLIRVPLGMWTDKFGGRIVFFV
LMLTTVIPIWLISYATEFWQFLLVGLFVGLAGGSFTVGIAYCARWFPRERQGLAMGIFGAGNTGAAVTKLLAPIIVVGYG
WTMVPKVYAGLMLVTAIVFWFFTYTEPAHKVSKSVTIREQLAAFKDPKVWRYSQYYSIVFGGYVALALWMTKYYVSQYGF
DLKTAAFLAAAFSIPGGVLRAVGGYFSDKYGAHTITWWVLWVSLLCLFFLSYPQSSITIHTVDGLRTFHFGLGPTAFTIV
MFSLGIAFAIGKASVFKYISDDYPDNIGVISGVVGLAGGLGGFLMPIMFGALVDLTGVRSSAFMLMFGVVWVSLMWMYFT
EVREMDAAARGAKRVIAADVME

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=442, Percent_Identity=26.2443438914027, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787475, Length=460, Percent_Identity=25.6521739130435, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46554; Mature: 46554

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure
MQSQQYKAWSVVSMSTLAFTVCFMIWMMFAVIGVPIKQQLGLNETQFGILVATPVLTGSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEECHHHHCHH
IRVPLGMWTDKFGGRIVFFVLMLTTVIPIWLISYATEFWQFLLVGLFVGLAGGSFTVGIA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YCARWFPRERQGLAMGIFGAGNTGAAVTKLLAPIIVVGYGWTMVPKVYAGLMLVTAIVFW
HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FFTYTEPAHKVSKSVTIREQLAAFKDPKVWRYSQYYSIVFGGYVALALWMTKYYVSQYGF
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DLKTAAFLAAAFSIPGGVLRAVGGYFSDKYGAHTITWWVLWVSLLCLFFLSYPQSSITIH
CHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TVDGLRTFHFGLGPTAFTIVMFSLGIAFAIGKASVFKYISDDYPDNIGVISGVVGLAGGL
EECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
GGFLMPIMFGALVDLTGVRSSAFMLMFGVVWVSLMWMYFTEVREMDAAARGAKRVIAADV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ME
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]