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The map label for this gene is mltF

Identifier: 74317248

GI number: 74317248

Start: 1288506

End: 1289918

Strand: Reverse

Name: mltF

Synonym: Tbd_1230

Alternate gene names: 74317248

Gene position: 1289918-1288506 (Counterclockwise)

Preceding gene: 74317249

Following gene: 74317245

Centisome position: 44.33

GC content: 66.53

Gene sequence:

>1413_bases
ATGCCCAGCCTCAAGACAAAGGGAGCGGCCGGGAAATTCGCTTCGTTGCTGCTGGTGCTCGCGCTGAGCGCCTGCAGCCG
GCCCGCGCCGCCGCCGGAAACGAGCGGCGAATTGCGCGTCGGGACACGCAACAGTCCGGCCACCTTTTACATCGGCCACG
ACGGCGAAACCGCGGGATTCGAACACGACCTCATCCTCGCATTCAGCCGCGCACAAAACTGGACGCTCAGTTGGACCGAA
AAGTCCCGGCCACAGGCGCTGTTCGACATGCTCGAGCGGCGCGAAATCCATCTCGCGGCGGCTGCGCTGCCGCAGGCCGT
GGTCAAGGACCGCCACCTGATCTCCGGTCCGATCCTGTTCGAGACCCCAGTCCACGTCGTCTATCGCACAGCCGATCGCG
CGCCGCGCGGCGTCGCCGGACTCGCCGGAAAAAAACTCGCATTCATCATCGGCTCCGGGCACGGCCCGATGCTCATGCGG
CTGAAACGCAAGCACCCGGAACTCAGCTGGGCCGCGGTGGAGAACGTCTGGCCCGAGGAATTGCTCGCGCAACTGCAGGC
GGGCAAGTACGACGCGGTGATCATCAACGGCATGGATTTCGACGCGATGCGCAACTTCTATCCCGGCCTCGCCGTCGCCT
TCGATCTGCCGTACAAGCAAAAAATCGTCTGGGCGCTGTCGCCCGGCAGTTCGCACGCCTTTCGCAACGCGCTCGCACGC
TTCGTCGAACGCGCGCGCTCGGACGGCACGATCAAGCGCGCGCTGGAACGCTACTTCGGGCACGTCAAGCGCCTGGGCAG
CAGCGACATTCTCGGCATCCTGCAACGCCGCCCGCAACGCCTTCCAGACCTGCGCGAGCATTTCCAGGAAGCGCAGACGC
TGAGCGGAATCGACTGGCGTCTGCTCGCCGCGATCGGCTACCAGGAATCGCAGTGGAACCGTCTGGCGACCTCGCCAACC
GGCGTGCGCGGCGTGATGATGCTGACCGGCGAGACGGCGGACCGCATGGGGGTCAGCGACCGCCTCAACGCGCGCGAGAG
CATCCTCGGCGGCGCACGCTATCTGGCGCTGTTGAAGGACGCGCTTCCCGCACGCATCGCCGAGCCGGATCGCACTTGGC
TCGCGCTCGCTGCCTACAACCAGGGTCAGGGCCACCTCGAGGACGCGCGGCGGATCGCGCAGGCACGCGGCGGCGACCCG
AACAGCTGGGCGGACGTCAAGGAGGCGCTGCCCTACCTGAGCCGCGGCAGCTACGCGAAGGTCATGAAATACGGCTATGC
GCGCGGCGGCGAAGCGCTGCGCTTCGCCGAGAACATCCGCAACTACTACGACATCCTGCTGCGGCTCGAGCCCGAATACG
ATCCCTTGATCAATCTCGGGCGAGGCGAAGACGGCCTGCCACCGCCGGGCTGA

Upstream 100 bases:

>100_bases
TCGACCACTCTGCCACCTCTCCGGCGGCGCGTATTGTAACCGAGTCGGCGCCAGCGCGCGCCTGCTGGACGAAAATTTCA
CACTCGGTCAGACTGCGCGC

Downstream 100 bases:

>100_bases
GACCTCAGGCCGGCGTCAGCCGCTCGACACCGCCCATCCACGGACGCAAGGCCTCGGGGACCGTGATGCTGCCGTCGGCG
TTCTGATAGTTTTCGAGGAT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MPSLKTKGAAGKFASLLLVLALSACSRPAPPPETSGELRVGTRNSPATFYIGHDGETAGFEHDLILAFSRAQNWTLSWTE
KSRPQALFDMLERREIHLAAAALPQAVVKDRHLISGPILFETPVHVVYRTADRAPRGVAGLAGKKLAFIIGSGHGPMLMR
LKRKHPELSWAAVENVWPEELLAQLQAGKYDAVIINGMDFDAMRNFYPGLAVAFDLPYKQKIVWALSPGSSHAFRNALAR
FVERARSDGTIKRALERYFGHVKRLGSSDILGILQRRPQRLPDLREHFQEAQTLSGIDWRLLAAIGYQESQWNRLATSPT
GVRGVMMLTGETADRMGVSDRLNARESILGGARYLALLKDALPARIAEPDRTWLALAAYNQGQGHLEDARRIAQARGGDP
NSWADVKEALPYLSRGSYAKVMKYGYARGGEALRFAENIRNYYDILLRLEPEYDPLINLGRGEDGLPPPG

Sequences:

>Translated_470_residues
MPSLKTKGAAGKFASLLLVLALSACSRPAPPPETSGELRVGTRNSPATFYIGHDGETAGFEHDLILAFSRAQNWTLSWTE
KSRPQALFDMLERREIHLAAAALPQAVVKDRHLISGPILFETPVHVVYRTADRAPRGVAGLAGKKLAFIIGSGHGPMLMR
LKRKHPELSWAAVENVWPEELLAQLQAGKYDAVIINGMDFDAMRNFYPGLAVAFDLPYKQKIVWALSPGSSHAFRNALAR
FVERARSDGTIKRALERYFGHVKRLGSSDILGILQRRPQRLPDLREHFQEAQTLSGIDWRLLAAIGYQESQWNRLATSPT
GVRGVMMLTGETADRMGVSDRLNARESILGGARYLALLKDALPARIAEPDRTWLALAAYNQGQGHLEDARRIAQARGGDP
NSWADVKEALPYLSRGSYAKVMKYGYARGGEALRFAENIRNYYDILLRLEPEYDPLINLGRGEDGLPPPG
>Mature_469_residues
PSLKTKGAAGKFASLLLVLALSACSRPAPPPETSGELRVGTRNSPATFYIGHDGETAGFEHDLILAFSRAQNWTLSWTEK
SRPQALFDMLERREIHLAAAALPQAVVKDRHLISGPILFETPVHVVYRTADRAPRGVAGLAGKKLAFIIGSGHGPMLMRL
KRKHPELSWAAVENVWPEELLAQLQAGKYDAVIINGMDFDAMRNFYPGLAVAFDLPYKQKIVWALSPGSSHAFRNALARF
VERARSDGTIKRALERYFGHVKRLGSSDILGILQRRPQRLPDLREHFQEAQTLSGIDWRLLAAIGYQESQWNRLATSPTG
VRGVMMLTGETADRMGVSDRLNARESILGGARYLALLKDALPARIAEPDRTWLALAAYNQGQGHLEDARRIAQARGGDPN
SWADVKEALPYLSRGSYAKVMKYGYARGGEALRFAENIRNYYDILLRLEPEYDPLINLGRGEDGLPPPG

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=408, Percent_Identity=37.5, Blast_Score=258, Evalue=8e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_THIDA (Q3SJH8)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314988.1
- ProteinModelPortal:   Q3SJH8
- SMR:   Q3SJH8
- STRING:   Q3SJH8
- GeneID:   3673319
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1230
- NMPDR:   fig|292415.3.peg.1250
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- PhylomeDB:   Q3SJH8
- BioCyc:   TDEN292415:TBD_1230-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 52024; Mature: 51893

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLKTKGAAGKFASLLLVLALSACSRPAPPPETSGELRVGTRNSPATFYIGHDGETAGF
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCC
EHDLILAFSRAQNWTLSWTEKSRPQALFDMLERREIHLAAAALPQAVVKDRHLISGPILF
CCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEE
ETPVHVVYRTADRAPRGVAGLAGKKLAFIIGSGHGPMLMRLKRKHPELSWAAVENVWPEE
ECCHHHEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHCCCHHH
LLAQLQAGKYDAVIINGMDFDAMRNFYPGLAVAFDLPYKQKIVWALSPGSSHAFRNALAR
HHHHHHCCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHH
FVERARSDGTIKRALERYFGHVKRLGSSDILGILQRRPQRLPDLREHFQEAQTLSGIDWR
HHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCE
LLAAIGYQESQWNRLATSPTGVRGVMMLTGETADRMGVSDRLNARESILGGARYLALLKD
EHHHHCCCHHHCCHHCCCCCCCCEEEEEECCCHHHCCCHHHCCHHHHHHHHHHHHHHHHH
ALPARIAEPDRTWLALAAYNQGQGHLEDARRIAQARGGDPNSWADVKEALPYLSRGSYAK
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHH
VMKYGYARGGEALRFAENIRNYYDILLRLEPEYDPLINLGRGEDGLPPPG
HHHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
PSLKTKGAAGKFASLLLVLALSACSRPAPPPETSGELRVGTRNSPATFYIGHDGETAGF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCC
EHDLILAFSRAQNWTLSWTEKSRPQALFDMLERREIHLAAAALPQAVVKDRHLISGPILF
CCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEE
ETPVHVVYRTADRAPRGVAGLAGKKLAFIIGSGHGPMLMRLKRKHPELSWAAVENVWPEE
ECCHHHEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHCCCHHH
LLAQLQAGKYDAVIINGMDFDAMRNFYPGLAVAFDLPYKQKIVWALSPGSSHAFRNALAR
HHHHHHCCCCCEEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHH
FVERARSDGTIKRALERYFGHVKRLGSSDILGILQRRPQRLPDLREHFQEAQTLSGIDWR
HHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCE
LLAAIGYQESQWNRLATSPTGVRGVMMLTGETADRMGVSDRLNARESILGGARYLALLKD
EHHHHCCCHHHCCHHCCCCCCCCEEEEEECCCHHHCCCHHHCCHHHHHHHHHHHHHHHHH
ALPARIAEPDRTWLALAAYNQGQGHLEDARRIAQARGGDPNSWADVKEALPYLSRGSYAK
HHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHH
VMKYGYARGGEALRFAENIRNYYDILLRLEPEYDPLINLGRGEDGLPPPG
HHHHHCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA