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The map label for this gene is queF

Identifier: 74317245

GI number: 74317245

Start: 1283138

End: 1283557

Strand: Reverse

Name: queF

Synonym: Tbd_1227

Alternate gene names: 74317245

Gene position: 1283557-1283138 (Counterclockwise)

Preceding gene: 74317248

Following gene: 74317244

Centisome position: 44.11

GC content: 62.86

Gene sequence:

>420_bases
ATGCCCTCCACGCCGAGCAAGACCCTCGAAACCTTCCCCAACCCCAAGCCCGGACGCGACTTTCACATCCACATGGAGGT
CCCTGAATTCACGTGTCTTTGCCCCAAGACCGGGCAGCCCGATTTCGCCACGCTCGTGCTCGACTACATCCCCAACCAGG
CCTGCGTCGAACTCAAGAGCCTGAAACTCTACATGTGGAGCTTCCGCGACGAGGGGCATTTCCACGAGGACGTCACCAAC
CGCATCCTCGATGACCTCGTCGCCGCGACGGACCCGCGCTACATGCGGCTGACCGCCAAGTTCTACGTGCGCGGCGGCAT
CTTCACCAACGTCGTTGCCGAGCATCGCAAGGACGGCTGGCAGCCGGCGCCGCGCGTGGACCTGACGCAGTTCGAGCACC
AGTCGAATACGCGCGGATAA

Upstream 100 bases:

>100_bases
GTTAAGCGCAAGACCGTGCAACCTTAGCTTTATATAATGGTGTCCCCGAGCGTCCCGGGCGGACGCTTTTTCCGCCGCCC
ATTCTACACCGAGCTTTTTT

Downstream 100 bases:

>100_bases
TTCCTTGTATTTCCTCGGAATCGACTTCGGCACCACGGGCGCCCGCGCCTGTGTCATCGACGCCCAGGGCGCGGTCGTCG
TCGAGGATAAACGCGACTTC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MPSTPSKTLETFPNPKPGRDFHIHMEVPEFTCLCPKTGQPDFATLVLDYIPNQACVELKSLKLYMWSFRDEGHFHEDVTN
RILDDLVAATDPRYMRLTAKFYVRGGIFTNVVAEHRKDGWQPAPRVDLTQFEHQSNTRG

Sequences:

>Translated_139_residues
MPSTPSKTLETFPNPKPGRDFHIHMEVPEFTCLCPKTGQPDFATLVLDYIPNQACVELKSLKLYMWSFRDEGHFHEDVTN
RILDDLVAATDPRYMRLTAKFYVRGGIFTNVVAEHRKDGWQPAPRVDLTQFEHQSNTRG
>Mature_138_residues
PSTPSKTLETFPNPKPGRDFHIHMEVPEFTCLCPKTGQPDFATLVLDYIPNQACVELKSLKLYMWSFRDEGHFHEDVTNR
ILDDLVAATDPRYMRLTAKFYVRGGIFTNVVAEHRKDGWQPAPRVDLTQFEHQSNTRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_THIDA (Q3SJI1)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314985.1
- ProteinModelPortal:   Q3SJI1
- SMR:   Q3SJI1
- STRING:   Q3SJI1
- GeneID:   3673316
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1227
- NMPDR:   fig|292415.3.peg.1247
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- BioCyc:   TDEN292415:TBD_1227-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 16023; Mature: 15892

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTPSKTLETFPNPKPGRDFHIHMEVPEFTCLCPKTGQPDFATLVLDYIPNQACVELKS
CCCCCCCHHHHCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHH
LKLYMWSFRDEGHFHEDVTNRILDDLVAATDPRYMRLTAKFYVRGGIFTNVVAEHRKDGW
HEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCC
QPAPRVDLTQFEHQSNTRG
CCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
PSTPSKTLETFPNPKPGRDFHIHMEVPEFTCLCPKTGQPDFATLVLDYIPNQACVELKS
CCCCCCHHHHCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHH
LKLYMWSFRDEGHFHEDVTNRILDDLVAATDPRYMRLTAKFYVRGGIFTNVVAEHRKDGW
HEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCC
QPAPRVDLTQFEHQSNTRG
CCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA