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The map label for this gene is pgdS [H]

Identifier: 74317010

GI number: 74317010

Start: 1058017

End: 1058529

Strand: Reverse

Name: pgdS [H]

Synonym: Tbd_0992

Alternate gene names: 74317010

Gene position: 1058529-1058017 (Counterclockwise)

Preceding gene: 74317013

Following gene: 74317009

Centisome position: 36.38

GC content: 66.86

Gene sequence:

>513_bases
GTGACCGTTTCCGTTTCAACTATAGAGCAGATTGTTTCGATGAACCGCGCCCTCCTGCCCCTGCTGGCCTTGCTGGCGCT
TCCCGTCGCGGCGGCAGACGTGCCGGCGGATGTCCCGATCTACGCCCTGAGTCTGATCGGGCACCCTTACCGGCTCGGCG
GCACCTCGCCCGAGACAGGGCTCGACTGCAGCGGCTTCGTCGCTCACGTCTACGGACAGGTGACCGGGGTCGTCCTGCCG
CGCGACAGCCGTTCGATCAGCACGCAGACGATTCCGCTCGCGCCGGACGAACTCCTGCCCGGCGATCTTGTCTTCTTCAA
CACCCTCGAGCGCGCGTTTTCCCATGTCGGCATTTATCTGGGCGATGGCCGTTTCGTGCACGCGGCGAGCAGCCGCAGCG
GCGCGGTCATGGTCTCCCGTATCGGCGACCCGTACTGGCGTGAACGCTACGACGGCGCGCGCCGCGTCGTCGTGCCGGAC
GCCGGGCAACCGGCCGCAGATGATCCGCGCTAA

Upstream 100 bases:

>100_bases
AGTTTCGCCAACTGCATGTTGTCGGGAATGGTGCTGCCGGCGGTGCCTGGCGCGGGCGCGTCGTTCATGATGCTGTCCTC
GTGTATAAGATTCACGACCT

Downstream 100 bases:

>100_bases
GCCCCGCCTCGGCCGCCGCGTACTCGCGGCGCTGGCACTGGTCGCAGCGGTACCGACCGCGGCCAGCGACGACTTCGACA
TGCGTAGCGCGGACGCCGTC

Product: hypothetical protein

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTVSVSTIEQIVSMNRALLPLLALLALPVAAADVPADVPIYALSLIGHPYRLGGTSPETGLDCSGFVAHVYGQVTGVVLP
RDSRSISTQTIPLAPDELLPGDLVFFNTLERAFSHVGIYLGDGRFVHAASSRSGAVMVSRIGDPYWRERYDGARRVVVPD
AGQPAADDPR

Sequences:

>Translated_170_residues
MTVSVSTIEQIVSMNRALLPLLALLALPVAAADVPADVPIYALSLIGHPYRLGGTSPETGLDCSGFVAHVYGQVTGVVLP
RDSRSISTQTIPLAPDELLPGDLVFFNTLERAFSHVGIYLGDGRFVHAASSRSGAVMVSRIGDPYWRERYDGARRVVVPD
AGQPAADDPR
>Mature_169_residues
TVSVSTIEQIVSMNRALLPLLALLALPVAAADVPADVPIYALSLIGHPYRLGGTSPETGLDCSGFVAHVYGQVTGVVLPR
DSRSISTQTIPLAPDELLPGDLVFFNTLERAFSHVGIYLGDGRFVHAASSRSGAVMVSRIGDPYWRERYDGARRVVVPDA
GQPAADDPR

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA) [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133 [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=122, Percent_Identity=40.1639344262295, Blast_Score=100, Evalue=4e-23,
Organism=Escherichia coli, GI1786421, Length=129, Percent_Identity=36.4341085271318, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788501, Length=132, Percent_Identity=39.3939393939394, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788001, Length=114, Percent_Identity=40.3508771929825, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 18111; Mature: 17980

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSVSTIEQIVSMNRALLPLLALLALPVAAADVPADVPIYALSLIGHPYRLGGTSPETG
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEECCCCCCCCC
LDCSGFVAHVYGQVTGVVLPRDSRSISTQTIPLAPDELLPGDLVFFNTLERAFSHVGIYL
CCCHHHHHHHHHHHEEEEECCCCCCCCCEECCCCCHHCCCCCHHHHHHHHHHHHHCCEEE
GDGRFVHAASSRSGAVMVSRIGDPYWRERYDGARRVVVPDAGQPAADDPR
CCCEEEEECCCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TVSVSTIEQIVSMNRALLPLLALLALPVAAADVPADVPIYALSLIGHPYRLGGTSPETG
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEECCCCCCCCC
LDCSGFVAHVYGQVTGVVLPRDSRSISTQTIPLAPDELLPGDLVFFNTLERAFSHVGIYL
CCCHHHHHHHHHHHEEEEECCCCCCCCCEECCCCCHHCCCCCHHHHHHHHHHHHHCCEEE
GDGRFVHAASSRSGAVMVSRIGDPYWRERYDGARRVVVPDAGQPAADDPR
CCCEEEEECCCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653 [H]