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The map label for this gene is prfC

Identifier: 74316986

GI number: 74316986

Start: 1030200

End: 1031831

Strand: Reverse

Name: prfC

Synonym: Tbd_0968

Alternate gene names: 74316986

Gene position: 1031831-1030200 (Counterclockwise)

Preceding gene: 74316987

Following gene: 74316985

Centisome position: 35.46

GC content: 65.81

Gene sequence:

>1632_bases
ATGGAATCCGCTTCCCCCGCACCGGCCCAGGCCGGCGACCCGGCCCTTGCGCGCGACGTCAAGAAGCGCCGCACGTTCGC
GATCATCTCCCACCCGGACGCCGGCAAGACGACGCTGACCGAAAAGCTGCTGCTGTTCGGCGGCGCGATCCAGATGGCCG
GCACGGTCAAGGCGAAGAAGAGCGGCAAGTTCGCCACCTCGGACTGGATGGCGGTCGAACAGGAGCGCGGCATCTCGGTC
GCCTCGTCGGTCATGCAGTTCTCCTACGGCGACTGCGTGTTCAACCTGCTCGACACGCCGGGCCACAAGGACTTCTCGGA
AGACACCTATCGCGTGCTGACCGCGGTCGACGCGGCGATCATGGTCGTCGACGCCGCGAAGGGCGTCGAAGAACAGACGA
TCAAGCTGCTCGAAGTCTGCCGACTGCGCGACACGCCGATCATCACCTTCATCAACAAGCTGGACCGCGAGGTGCGCGAG
CCGCTCGAGCTGTTGGACGAGATCGAGTCGATCCTGAAGATCCACTGCGCGCCAGTGACCTGGCCGATCGGCATGGGCAA
GCGCTTCCAGGGCGTGTATCACCTGATCCACGACGAGATCCTGCGGTTCAAGGCAGGCGAGGAAAACGCGGGCCAGCAGT
TCGAGTCGCTGCAGGGCCTCGGCGACGCGAAGCTGCGCGAGCTCTTTCCGCTCGAGGCCGACGCGCTGCTGTCCGAGATC
GAACTCGTTCGCGGGGCCGGCGCGGCCTTCGACCTGGCAGACTTTCTCGCTGGCCGCCAGACGCCCGTGTTCTTCGGCTC
CGGCATCAACAACTTCGGCGTGCGCGAGGTGCTGCGCGCACTGTCGGACTGGGCGCCCTCGCCACTGCCGCGCGATGCGG
TCGAGCGCACGGTCGAGCCGACCGAACCGAAGTTCACCGGTTTCGTCTTCAAGATTCAGGCCAACATGGACCCGCAGCAC
CGCGACCGCATCGCGTTTTTCCGCGTCTGCTCTGGCCGCTATTCGCCGGGTATGAAGGTGCGCCACCGGCGCACCGGCAA
GGACATGAAGATCGCCAACGCGGTCGTGTTCATGGCCAACGAGCGGCAGCGCTCGGACGACGCCGTCGCGGGCGACATCA
TCGGCATCCACAACCACGGCCAGCTGCAGATCGGCGACACGCTGACCGAAGGCGAGGCGCTGCAGTTCAAGGGCATCCCC
TACTTCGCGCCCGAATTGTTCTCCAAGCCGCGGCTCCGCGATCCCTTGCGCGCCAAGCAGCTCAACAAGGGCCTGCTCGA
ACTCGGCGAGGAAGGCGCGGTGCAGGTGTTCGAGCCCTTCGCCGACAACACGCTCTTGATCGGCGCAGTCGGCCCGCTGC
AGTTCGAGATCGTCGCGCACCGATTGAAGACCGAATACGGCGTCGACGCGAGCTTCGAGAACGCGGGGATTCACACGGCG
CGGTGGGTCACCTGCCCCGACGCCCAGCACCTCGCCGAATTCACCAAGGCCAATTCGCTGCGGCTCGCCAAGGACGTCGA
CGGCAATCTCGTGTATCTGGCCGACACGCGCGTGAACCTCACCCTCGCCCAGGAACGTTGGCCCAAGGTCGCGTTCCACG
ACACGCGCGAGCACGGACAGCTCCTGACTTGA

Upstream 100 bases:

>100_bases
CGTGACGGCAACCCCGCACCCTGCGATATCGTCATCCACGCCGTAGGGAAGCACGCGGAATCGCTTAAAATTGCGCCTGC
CTCAGTATCAGAACACCCCG

Downstream 100 bases:

>100_bases
GTCGCACGTTCCGTATGCGGCGCGGCCGTTGAAGAACACGCCTGAGGATCGCGCGGTCGCGGTGATCGCGCGGTAACGCA
AGCGGCGCGCTTCGTGACAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MESASPAPAQAGDPALARDVKKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGTVKAKKSGKFATSDWMAVEQERGISV
ASSVMQFSYGDCVFNLLDTPGHKDFSEDTYRVLTAVDAAIMVVDAAKGVEEQTIKLLEVCRLRDTPIITFINKLDREVRE
PLELLDEIESILKIHCAPVTWPIGMGKRFQGVYHLIHDEILRFKAGEENAGQQFESLQGLGDAKLRELFPLEADALLSEI
ELVRGAGAAFDLADFLAGRQTPVFFGSGINNFGVREVLRALSDWAPSPLPRDAVERTVEPTEPKFTGFVFKIQANMDPQH
RDRIAFFRVCSGRYSPGMKVRHRRTGKDMKIANAVVFMANERQRSDDAVAGDIIGIHNHGQLQIGDTLTEGEALQFKGIP
YFAPELFSKPRLRDPLRAKQLNKGLLELGEEGAVQVFEPFADNTLLIGAVGPLQFEIVAHRLKTEYGVDASFENAGIHTA
RWVTCPDAQHLAEFTKANSLRLAKDVDGNLVYLADTRVNLTLAQERWPKVAFHDTREHGQLLT

Sequences:

>Translated_543_residues
MESASPAPAQAGDPALARDVKKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGTVKAKKSGKFATSDWMAVEQERGISV
ASSVMQFSYGDCVFNLLDTPGHKDFSEDTYRVLTAVDAAIMVVDAAKGVEEQTIKLLEVCRLRDTPIITFINKLDREVRE
PLELLDEIESILKIHCAPVTWPIGMGKRFQGVYHLIHDEILRFKAGEENAGQQFESLQGLGDAKLRELFPLEADALLSEI
ELVRGAGAAFDLADFLAGRQTPVFFGSGINNFGVREVLRALSDWAPSPLPRDAVERTVEPTEPKFTGFVFKIQANMDPQH
RDRIAFFRVCSGRYSPGMKVRHRRTGKDMKIANAVVFMANERQRSDDAVAGDIIGIHNHGQLQIGDTLTEGEALQFKGIP
YFAPELFSKPRLRDPLRAKQLNKGLLELGEEGAVQVFEPFADNTLLIGAVGPLQFEIVAHRLKTEYGVDASFENAGIHTA
RWVTCPDAQHLAEFTKANSLRLAKDVDGNLVYLADTRVNLTLAQERWPKVAFHDTREHGQLLT
>Mature_543_residues
MESASPAPAQAGDPALARDVKKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGTVKAKKSGKFATSDWMAVEQERGISV
ASSVMQFSYGDCVFNLLDTPGHKDFSEDTYRVLTAVDAAIMVVDAAKGVEEQTIKLLEVCRLRDTPIITFINKLDREVRE
PLELLDEIESILKIHCAPVTWPIGMGKRFQGVYHLIHDEILRFKAGEENAGQQFESLQGLGDAKLRELFPLEADALLSEI
ELVRGAGAAFDLADFLAGRQTPVFFGSGINNFGVREVLRALSDWAPSPLPRDAVERTVEPTEPKFTGFVFKIQANMDPQH
RDRIAFFRVCSGRYSPGMKVRHRRTGKDMKIANAVVFMANERQRSDDAVAGDIIGIHNHGQLQIGDTLTEGEALQFKGIP
YFAPELFSKPRLRDPLRAKQLNKGLLELGEEGAVQVFEPFADNTLLIGAVGPLQFEIVAHRLKTEYGVDASFENAGIHTA
RWVTCPDAQHLAEFTKANSLRLAKDVDGNLVYLADTRVNLTLAQERWPKVAFHDTREHGQLLT

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=28.0737704918033, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI19923640, Length=508, Percent_Identity=26.3779527559055, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=27.3657289002558, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306287, Length=483, Percent_Identity=26.7080745341615, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI157426893, Length=173, Percent_Identity=32.9479768786127, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=166, Percent_Identity=32.5301204819277, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI310132016, Length=121, Percent_Identity=34.7107438016529, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310110807, Length=121, Percent_Identity=34.7107438016529, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI310123363, Length=121, Percent_Identity=34.7107438016529, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=31.5436241610738, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=59.7701149425287, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=485, Percent_Identity=28.0412371134021, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=33.125, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI48994988, Length=184, Percent_Identity=31.5217391304348, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=512, Percent_Identity=28.125, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=32.5966850828729, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=34.8684210526316, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=156, Percent_Identity=30.1282051282051, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=156, Percent_Identity=30.1282051282051, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=174, Percent_Identity=29.8850574712644, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=496, Percent_Identity=29.0322580645161, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=516, Percent_Identity=24.4186046511628, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=162, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=162, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=30.4635761589404, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=27.5720164609054, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI221458488, Length=511, Percent_Identity=24.0704500978474, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=34.5911949685535, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=162, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585711, Length=162, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585713, Length=162, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574573, Length=154, Percent_Identity=34.4155844155844, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_THIDA (Q3SK69)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314726.1
- ProteinModelPortal:   Q3SK69
- SMR:   Q3SK69
- STRING:   Q3SK69
- GeneID:   3672760
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0968
- NMPDR:   fig|292415.3.peg.595
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- PhylomeDB:   Q3SK69
- ProtClustDB:   PRK00741
- BioCyc:   TDEN292415:TBD_0968-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 60103; Mature: 60103

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESASPAPAQAGDPALARDVKKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGTVKAKK
CCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCEEEEECC
SGKFATSDWMAVEQERGISVASSVMQFSYGDCVFNLLDTPGHKDFSEDTYRVLTAVDAAI
CCCCCCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MVVDAAKGVEEQTIKLLEVCRLRDTPIITFINKLDREVREPLELLDEIESILKIHCAPVT
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEE
WPIGMGKRFQGVYHLIHDEILRFKAGEENAGQQFESLQGLGDAKLRELFPLEADALLSEI
EECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
ELVRGAGAAFDLADFLAGRQTPVFFGSGINNFGVREVLRALSDWAPSPLPRDAVERTVEP
HHHHCCCCHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCC
TEPKFTGFVFKIQANMDPQHRDRIAFFRVCSGRYSPGMKVRHRRTGKDMKIANAVVFMAN
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEEEEEEEC
ERQRSDDAVAGDIIGIHNHGQLQIGDTLTEGEALQFKGIPYFAPELFSKPRLRDPLRAKQ
CCCCCCCCCCCCEEEECCCCCEEECCCCCCCCEEEECCCCCCCHHHCCCCCCCCCHHHHH
LNKGLLELGEEGAVQVFEPFADNTLLIGAVGPLQFEIVAHRLKTEYGVDASFENAGIHTA
HHHHHHHCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
RWVTCPDAQHLAEFTKANSLRLAKDVDGNLVYLADTRVNLTLAQERWPKVAFHDTREHGQ
EEEECCCHHHHHHHHHCCCEEEEECCCCCEEEEEECEEEEEECHHHCCCCEECCCCCCCC
LLT
CCC
>Mature Secondary Structure
MESASPAPAQAGDPALARDVKKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGTVKAKK
CCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCEEEEECC
SGKFATSDWMAVEQERGISVASSVMQFSYGDCVFNLLDTPGHKDFSEDTYRVLTAVDAAI
CCCCCCCCCHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MVVDAAKGVEEQTIKLLEVCRLRDTPIITFINKLDREVREPLELLDEIESILKIHCAPVT
EEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEE
WPIGMGKRFQGVYHLIHDEILRFKAGEENAGQQFESLQGLGDAKLRELFPLEADALLSEI
EECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
ELVRGAGAAFDLADFLAGRQTPVFFGSGINNFGVREVLRALSDWAPSPLPRDAVERTVEP
HHHHCCCCHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHCCCC
TEPKFTGFVFKIQANMDPQHRDRIAFFRVCSGRYSPGMKVRHRRTGKDMKIANAVVFMAN
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEEEEEEEEC
ERQRSDDAVAGDIIGIHNHGQLQIGDTLTEGEALQFKGIPYFAPELFSKPRLRDPLRAKQ
CCCCCCCCCCCCEEEECCCCCEEECCCCCCCCEEEECCCCCCCHHHCCCCCCCCCHHHHH
LNKGLLELGEEGAVQVFEPFADNTLLIGAVGPLQFEIVAHRLKTEYGVDASFENAGIHTA
HHHHHHHCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
RWVTCPDAQHLAEFTKANSLRLAKDVDGNLVYLADTRVNLTLAQERWPKVAFHDTREHGQ
EEEECCCHHHHHHHHHCCCEEEEECCCCCEEEEEECEEEEEECHHHCCCCEECCCCCCCC
LLT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA