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The map label for this gene is pleD [H]

Identifier: 74316654

GI number: 74316654

Start: 675129

End: 676808

Strand: Direct

Name: pleD [H]

Synonym: Tbd_0636

Alternate gene names: 74316654

Gene position: 675129-676808 (Clockwise)

Preceding gene: 74316653

Following gene: 74316665

Centisome position: 23.2

GC content: 68.39

Gene sequence:

>1680_bases
TTGACAAAGCAGCTGCCGCTTCGCGATAGTGGCTGCATAACGAGCGGAGGACGGCAAAATGCCCACCGAAAGGATCGCCG
CAGGCGATCGGTCGCCTCGTCGCGCCCACCTCGAGCCGACGGCTGCGTGAGTCAGTCGCTGCGGCACATGCTGTTGCTTC
CGCTCGCGACCGGGCTCGCGTATTACCTGGGCGCGGTGCTCGGCGTCGCCGCCTCCGCGATGTCGGAAGGCATCGCCATT
TTCTGGCTGCCGAACGGCATCCTGCTGGTCGTGCTGCTCTCGACGGCACGGCACCACTGGCCGCTGCATCTCGCCGCCGC
GGTGCTCGCCGAAATCGCGGCCGACCTTCCGACTTTCACCCTCCTCCAAGCGCTCGGTTTCGCCGCTGTCAACGTCGTCG
AATGCCTGCTCGCCGCGCTCCTGCTCGAGCGCCTCGCGCGGCCGTTCAGGCTCGAGAGCCTGCGCCACGTCATCGCTTTC
GGTGGCTACGTCTTGGTCATCGCCTGCGGCCTCGCCGCCTTGCTCGGCGCCGCGATCTATTCGCTGACAGGGCAGGCGAC
GACCGCGTTCTGGACCTACTGGCGGATATGGTGGTTCGGAGACGGACTGGGCATGCTTCTGGTCGCGCCGCTCGCGCTGG
GCTGGCTGCAGCCCCGGAGTCGGCCCGGCGAGCGCCGGTCTTTCGAAGCGACCGCCTTGTTCGGCCTCACCCTGCTGTTC
TCGCTATGGGTTTTTTACCCGTCCGCCGCCCATGTCGAAGCGGCCTTTCCGCGGCGCGCCTTCCTGCTGCTGCCGTTCGC
GCTCTGGGCGGCGGCGCGCTTCGGCGTGTGGGGCACGGCCAGCGTCGCACTGCTGCTCGCCGGCATCGCGATCCCCGCGA
CGCTCACCGATCGCGGCCCCCTTGCGCTGGCGGCGCCCGAGGCCACGGTGCTGTTGTTGCAGGAATATCTTGTAGTGCTG
AGCCTGCCGGCACTCGCGCTGGCGGCGCTCCTGCAGGAGCTGCGCGACCGGAACGCGCGCCTGCAGTCACGGGAGACCGA
ATTGCGCATGACGCACGATGCGCTCGATCGAGTCAATCGCGAACTCGAAACGCGGGTGCGCACACGGACCGAAGCGCTCG
AGGTCGCGAACCGTCGTCTCAAGGCGCTGGCCTCGGTCGACTCGCTGACGGGGATCAGCAACCGCCGTCATTTCGTCGAG
CAGGCCAAGGCCGAAATCAGCCGTGCCAGACGCCAAGGCCTGCCGCTGTCGGTGATCATGTTCGACATCGATTTCTTCAA
GACGGTCAACGACCGCTTCGGCCACGAGGCCGGGGACCAGACCTTGAAGGCGCTGACGAGCACGGTCAGCGGGAACCTTC
GTGCCGGCGACATTTTCGCGCGGCTCGGCGGCGAGGAGTTCATCCTGATGCTGCCGGGGCAGGGCCTCGGTGACGCCGTG
CAGACCGCCGACCGTGTGCGCGCGTTGGTCGCAGAGACGAGTCGCACCCACTACCCAGTGCCGATCACGCTCAGTGCGGG
TGTCGCCGGGCTGGCCGGATCGGAAGATCTCGACGGCCTGCTGCGCCAGGCGGACCGCGCGCTCTACGCGGCGAAGCACC
AGGGACGCGACCGCGTGTGTGTCGCGCCGGCGGCGGGTGCGCCCGAAACCCTGCCGAGCGCTGGGCCGTTCATGGGCTGA

Upstream 100 bases:

>100_bases
TACCTCAAGGAGGCGGCCGGTGAGCGGCCGCGCTATTCCCGCACGGCCTGAACCGCCCGCCGGCGTGTCCGGCGTCGCGC
ATCCCTGATCTCCGCTCCGG

Downstream 100 bases:

>100_bases
GCGTCAACAACAGCCGCCGGTGTCGCGCTTGGGACAGCCTTCGGGCCGGTCGGGCGGCGGCAGTGGACAACGATTGATCT
CGTCGGCGCTGCGCTGGAAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTKQLPLRDSGCITSGGRQNAHRKDRRRRSVASSRPPRADGCVSQSLRHMLLLPLATGLAYYLGAVLGVAASAMSEGIAI
FWLPNGILLVVLLSTARHHWPLHLAAAVLAEIAADLPTFTLLQALGFAAVNVVECLLAALLLERLARPFRLESLRHVIAF
GGYVLVIACGLAALLGAAIYSLTGQATTAFWTYWRIWWFGDGLGMLLVAPLALGWLQPRSRPGERRSFEATALFGLTLLF
SLWVFYPSAAHVEAAFPRRAFLLLPFALWAAARFGVWGTASVALLLAGIAIPATLTDRGPLALAAPEATVLLLQEYLVVL
SLPALALAALLQELRDRNARLQSRETELRMTHDALDRVNRELETRVRTRTEALEVANRRLKALASVDSLTGISNRRHFVE
QAKAEISRARRQGLPLSVIMFDIDFFKTVNDRFGHEAGDQTLKALTSTVSGNLRAGDIFARLGGEEFILMLPGQGLGDAV
QTADRVRALVAETSRTHYPVPITLSAGVAGLAGSEDLDGLLRQADRALYAAKHQGRDRVCVAPAAGAPETLPSAGPFMG

Sequences:

>Translated_559_residues
MTKQLPLRDSGCITSGGRQNAHRKDRRRRSVASSRPPRADGCVSQSLRHMLLLPLATGLAYYLGAVLGVAASAMSEGIAI
FWLPNGILLVVLLSTARHHWPLHLAAAVLAEIAADLPTFTLLQALGFAAVNVVECLLAALLLERLARPFRLESLRHVIAF
GGYVLVIACGLAALLGAAIYSLTGQATTAFWTYWRIWWFGDGLGMLLVAPLALGWLQPRSRPGERRSFEATALFGLTLLF
SLWVFYPSAAHVEAAFPRRAFLLLPFALWAAARFGVWGTASVALLLAGIAIPATLTDRGPLALAAPEATVLLLQEYLVVL
SLPALALAALLQELRDRNARLQSRETELRMTHDALDRVNRELETRVRTRTEALEVANRRLKALASVDSLTGISNRRHFVE
QAKAEISRARRQGLPLSVIMFDIDFFKTVNDRFGHEAGDQTLKALTSTVSGNLRAGDIFARLGGEEFILMLPGQGLGDAV
QTADRVRALVAETSRTHYPVPITLSAGVAGLAGSEDLDGLLRQADRALYAAKHQGRDRVCVAPAAGAPETLPSAGPFMG
>Mature_558_residues
TKQLPLRDSGCITSGGRQNAHRKDRRRRSVASSRPPRADGCVSQSLRHMLLLPLATGLAYYLGAVLGVAASAMSEGIAIF
WLPNGILLVVLLSTARHHWPLHLAAAVLAEIAADLPTFTLLQALGFAAVNVVECLLAALLLERLARPFRLESLRHVIAFG
GYVLVIACGLAALLGAAIYSLTGQATTAFWTYWRIWWFGDGLGMLLVAPLALGWLQPRSRPGERRSFEATALFGLTLLFS
LWVFYPSAAHVEAAFPRRAFLLLPFALWAAARFGVWGTASVALLLAGIAIPATLTDRGPLALAAPEATVLLLQEYLVVLS
LPALALAALLQELRDRNARLQSRETELRMTHDALDRVNRELETRVRTRTEALEVANRRLKALASVDSLTGISNRRHFVEQ
AKAEISRARRQGLPLSVIMFDIDFFKTVNDRFGHEAGDQTLKALTSTVSGNLRAGDIFARLGGEEFILMLPGQGLGDAVQ
TADRVRALVAETSRTHYPVPITLSAGVAGLAGSEDLDGLLRQADRALYAAKHQGRDRVCVAPAAGAPETLPSAGPFMG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=38.0368098159509, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI87082007, Length=185, Percent_Identity=36.7567567567568, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=38.7878787878788, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=35.1190476190476, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787262, Length=178, Percent_Identity=33.1460674157303, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=35.031847133758, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=35.2941176470588, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=32.0987654320988, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.7073170731707, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=31.6091954022989, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=182, Percent_Identity=30.2197802197802, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 60441; Mature: 60310

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQLPLRDSGCITSGGRQNAHRKDRRRRSVASSRPPRADGCVSQSLRHMLLLPLATGLA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YYLGAVLGVAASAMSEGIAIFWLPNGILLVVLLSTARHHWPLHLAAAVLAEIAADLPTFT
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
LLQALGFAAVNVVECLLAALLLERLARPFRLESLRHVIAFGGYVLVIACGLAALLGAAIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTGQATTAFWTYWRIWWFGDGLGMLLVAPLALGWLQPRSRPGERRSFEATALFGLTLLF
HHCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
SLWVFYPSAAHVEAAFPRRAFLLLPFALWAAARFGVWGTASVALLLAGIAIPATLTDRGP
HHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCCC
LALAAPEATVLLLQEYLVVLSLPALALAALLQELRDRNARLQSRETELRMTHDALDRVNR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELETRVRTRTEALEVANRRLKALASVDSLTGISNRRHFVEQAKAEISRARRQGLPLSVIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
FDIDFFKTVNDRFGHEAGDQTLKALTSTVSGNLRAGDIFARLGGEEFILMLPGQGLGDAV
EHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCCHHH
QTADRVRALVAETSRTHYPVPITLSAGVAGLAGSEDLDGLLRQADRALYAAKHQGRDRVC
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VAPAAGAPETLPSAGPFMG
EECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKQLPLRDSGCITSGGRQNAHRKDRRRRSVASSRPPRADGCVSQSLRHMLLLPLATGLA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YYLGAVLGVAASAMSEGIAIFWLPNGILLVVLLSTARHHWPLHLAAAVLAEIAADLPTFT
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHH
LLQALGFAAVNVVECLLAALLLERLARPFRLESLRHVIAFGGYVLVIACGLAALLGAAIY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLTGQATTAFWTYWRIWWFGDGLGMLLVAPLALGWLQPRSRPGERRSFEATALFGLTLLF
HHCCCHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
SLWVFYPSAAHVEAAFPRRAFLLLPFALWAAARFGVWGTASVALLLAGIAIPATLTDRGP
HHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCCC
LALAAPEATVLLLQEYLVVLSLPALALAALLQELRDRNARLQSRETELRMTHDALDRVNR
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ELETRVRTRTEALEVANRRLKALASVDSLTGISNRRHFVEQAKAEISRARRQGLPLSVIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
FDIDFFKTVNDRFGHEAGDQTLKALTSTVSGNLRAGDIFARLGGEEFILMLPGQGLGDAV
EHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCCCCHHH
QTADRVRALVAETSRTHYPVPITLSAGVAGLAGSEDLDGLLRQADRALYAAKHQGRDRVC
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VAPAAGAPETLPSAGPFMG
EECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA