Click here to switch to the map view.

The map label for this gene is hemN [H]

Identifier: 74316653

GI number: 74316653

Start: 673709

End: 675079

Strand: Direct

Name: hemN [H]

Synonym: Tbd_0635

Alternate gene names: 74316653

Gene position: 673709-675079 (Clockwise)

Preceding gene: 74316649

Following gene: 74316654

Centisome position: 23.15

GC content: 65.86

Gene sequence:

>1371_bases
ATGTCGTCGACCCCCCTCGAATTGCTCCAGAAATACAGCGTGCCCGGGCCGCGTTATACGTCCTACCCGACCGCGCCCTA
TTTCCACACCGGCTTCGGCGAGACCGACTGGCGCGAGGCGCTCGCGCGCGCGCGCAGCGATCGCGCCTTGTCGCTGTACG
CGCACGTGCCGTTCTGCGACTCGCTCTGCTACTACTGCGGCTGCAACATGGTCGCGACGCGGGACTACAGCAAGACCGTT
CCCTATCTTGCCGCGATCGAGCGCGAAATGGAGCGCACGGCCGCCCTGGTCGACCCGGGCCGGGTCGTGCGCCAGCTGCA
CTGGGGCGGCGGCACGCCGACCTACCTCAACCCCGACGACATCCGCCGCCTGATGGCGATGATGCAGCGCAATTTCACGA
TTGCGCCCGACGCCGAGATCAGCTGCGAGGTCGATCCGCGCGAACTGACCTACGAGCATCTGGCGGCCTTGCGCGAGACC
GGCTTCAATCGACTGTCGTTCGGCGTGCAGGACATGGACTCCGACGTGCAGCAGGCGGTCAACCGCATCCAGTCCGAATT
CCTCATCCGCCAGGTGCTCGACTGGGCGCGCGAACTCGGTTTTTCGAGCATCAACCTCGACCTGATCGTCGGCCTTCCGA
AACAGACCGTCGAGAGCTTCAGCCGCACGCTTGAGCGCGTCACGAACTGGGCGCCCGACCGCCTCGCGGTCTTCGCCTAT
GCGCACGTGCCTTGGATCAAGAAGCACCAGAACCTGATCAACGAGGCCGACCTTCCCGACGCCGCCACACGGCTCGGCCT
GCAGCAGGCCGTGAACGACGCGCTCGCCGCGGCCGGCTACGTCAATATCGGACTCGACCATTTCGCGCGGCCCGAGGACG
AGCTCGTGCGCGCGCAGCAGAACAAGACGCTGTGGCGGAATTTCCAGGGCTACACGACGCACAAGGACTGCGACATCCTG
GCCTTCGGCGTATCGAGCATCAGCCAGACCGACGATGTCTACATGCAGAACGACAAGAATCTCAAGCGCTACCAGGATCG
CGCGGCTGCGAACGGCTTCGCGGTCGAGCGCGGGCTGAGGCTCACGCGCGACGACCAGGTCCGTCGCGACGCGATCACGC
GCGTGATGTGCGACCTCGAACTCGATTTCGCCGCCTTTGGGAAGGAATGGGACGTCAGCTTCGGCGACTACTTCGCCGAC
GCCCTGGTCCAGCTACGCCCGCTGGCCGACGACGGTCTGGTCGACATCGCGGCGGACCGGATCAGCGTCACCGCGACGGG
CCGCCTTTTCCTGCGCAACATCGGCATGTGCTTCGACCGCTACCTCAAGGAGGCGGCCGGTGAGCGGCCGCGCTATTCCC
GCACGGCCTGA

Upstream 100 bases:

>100_bases
TTTGAACTTTAACCCAAAAAACGCGGGCCGACGGCGACGGCTGGATTTCGGCGCGCGTCTATGATAAAAAAGATATTGCC
ATCTGTTATAGGGCGCTTTG

Downstream 100 bases:

>100_bases
ACCGCCCGCCGGCGTGTCCGGCGTCGCGCATCCCTGATCTCCGCTCCGGTTGACAAAGCAGCTGCCGCTTCGCGATAGTG
GCTGCATAACGAGCGGAGGA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSSTPLELLQKYSVPGPRYTSYPTAPYFHTGFGETDWREALARARSDRALSLYAHVPFCDSLCYYCGCNMVATRDYSKTV
PYLAAIEREMERTAALVDPGRVVRQLHWGGGTPTYLNPDDIRRLMAMMQRNFTIAPDAEISCEVDPRELTYEHLAALRET
GFNRLSFGVQDMDSDVQQAVNRIQSEFLIRQVLDWARELGFSSINLDLIVGLPKQTVESFSRTLERVTNWAPDRLAVFAY
AHVPWIKKHQNLINEADLPDAATRLGLQQAVNDALAAAGYVNIGLDHFARPEDELVRAQQNKTLWRNFQGYTTHKDCDIL
AFGVSSISQTDDVYMQNDKNLKRYQDRAAANGFAVERGLRLTRDDQVRRDAITRVMCDLELDFAAFGKEWDVSFGDYFAD
ALVQLRPLADDGLVDIAADRISVTATGRLFLRNIGMCFDRYLKEAAGERPRYSRTA

Sequences:

>Translated_456_residues
MSSTPLELLQKYSVPGPRYTSYPTAPYFHTGFGETDWREALARARSDRALSLYAHVPFCDSLCYYCGCNMVATRDYSKTV
PYLAAIEREMERTAALVDPGRVVRQLHWGGGTPTYLNPDDIRRLMAMMQRNFTIAPDAEISCEVDPRELTYEHLAALRET
GFNRLSFGVQDMDSDVQQAVNRIQSEFLIRQVLDWARELGFSSINLDLIVGLPKQTVESFSRTLERVTNWAPDRLAVFAY
AHVPWIKKHQNLINEADLPDAATRLGLQQAVNDALAAAGYVNIGLDHFARPEDELVRAQQNKTLWRNFQGYTTHKDCDIL
AFGVSSISQTDDVYMQNDKNLKRYQDRAAANGFAVERGLRLTRDDQVRRDAITRVMCDLELDFAAFGKEWDVSFGDYFAD
ALVQLRPLADDGLVDIAADRISVTATGRLFLRNIGMCFDRYLKEAAGERPRYSRTA
>Mature_455_residues
SSTPLELLQKYSVPGPRYTSYPTAPYFHTGFGETDWREALARARSDRALSLYAHVPFCDSLCYYCGCNMVATRDYSKTVP
YLAAIEREMERTAALVDPGRVVRQLHWGGGTPTYLNPDDIRRLMAMMQRNFTIAPDAEISCEVDPRELTYEHLAALRETG
FNRLSFGVQDMDSDVQQAVNRIQSEFLIRQVLDWARELGFSSINLDLIVGLPKQTVESFSRTLERVTNWAPDRLAVFAYA
HVPWIKKHQNLINEADLPDAATRLGLQQAVNDALAAAGYVNIGLDHFARPEDELVRAQQNKTLWRNFQGYTTHKDCDILA
FGVSSISQTDDVYMQNDKNLKRYQDRAAANGFAVERGLRLTRDDQVRRDAITRVMCDLELDFAAFGKEWDVSFGDYFADA
LVQLRPLADDGLVDIAADRISVTATGRLFLRNIGMCFDRYLKEAAGERPRYSRTA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=196, Percent_Identity=26.0204081632653, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI87082341, Length=441, Percent_Identity=53.968253968254, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1789325, Length=391, Percent_Identity=25.5754475703325, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 51720; Mature: 51589

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPLELLQKYSVPGPRYTSYPTAPYFHTGFGETDWREALARARSDRALSLYAHVPFCD
CCCCHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEEECCHHH
SLCYYCGCNMVATRDYSKTVPYLAAIEREMERTAALVDPGRVVRQLHWGGGTPTYLNPDD
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHH
IRRLMAMMQRNFTIAPDAEISCEVDPRELTYEHLAALRETGFNRLSFGVQDMDSDVQQAV
HHHHHHHHHCCCEECCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHH
NRIQSEFLIRQVLDWARELGFSSINLDLIVGLPKQTVESFSRTLERVTNWAPDRLAVFAY
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHEEEHH
AHVPWIKKHQNLINEADLPDAATRLGLQQAVNDALAAAGYVNIGLDHFARPEDELVRAQQ
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHCCCHHHCCCCHHHHHHHHH
NKTLWRNFQGYTTHKDCDILAFGVSSISQTDDVYMQNDKNLKRYQDRAAANGFAVERGLR
HHHHHHCCCCCCCCCCCCEEEECHHHHCCCCCCEECCCCHHHHHHHHHHCCCHHHHCCCC
LTRDDQVRRDAITRVMCDLELDFAAFGKEWDVSFGDYFADALVQLRPLADDGLVDIAADR
CCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCC
ISVTATGRLFLRNIGMCFDRYLKEAAGERPRYSRTA
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SSTPLELLQKYSVPGPRYTSYPTAPYFHTGFGETDWREALARARSDRALSLYAHVPFCD
CCCHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEEECCHHH
SLCYYCGCNMVATRDYSKTVPYLAAIEREMERTAALVDPGRVVRQLHWGGGTPTYLNPDD
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHH
IRRLMAMMQRNFTIAPDAEISCEVDPRELTYEHLAALRETGFNRLSFGVQDMDSDVQQAV
HHHHHHHHHCCCEECCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHH
NRIQSEFLIRQVLDWARELGFSSINLDLIVGLPKQTVESFSRTLERVTNWAPDRLAVFAY
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHEEEHH
AHVPWIKKHQNLINEADLPDAATRLGLQQAVNDALAAAGYVNIGLDHFARPEDELVRAQQ
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHCCCHHHCCCCHHHHHHHHH
NKTLWRNFQGYTTHKDCDILAFGVSSISQTDDVYMQNDKNLKRYQDRAAANGFAVERGLR
HHHHHHCCCCCCCCCCCCEEEECHHHHCCCCCCEECCCCHHHHHHHHHHCCCHHHHCCCC
LTRDDQVRRDAITRVMCDLELDFAAFGKEWDVSFGDYFADALVQLRPLADDGLVDIAADR
CCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCC
ISVTATGRLFLRNIGMCFDRYLKEAAGERPRYSRTA
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768836; 8346018; 9278503 [H]