Click here to switch to the map view.

The map label for this gene is ntrC [H]

Identifier: 74316569

GI number: 74316569

Start: 577920

End: 579350

Strand: Reverse

Name: ntrC [H]

Synonym: Tbd_0551

Alternate gene names: 74316569

Gene position: 579350-577920 (Counterclockwise)

Preceding gene: 74316570

Following gene: 74316568

Centisome position: 19.91

GC content: 64.92

Gene sequence:

>1431_bases
GTGGTCCAAACCAACAAGCCGACTTTGCTGATCGTCGATGACGATCCGCTGATTTCCGACACTCTGACCTACGTTCTCAG
CAAGGACTTCGAAATCTCGGCCGCCGAGTCGCGCGCACAGGTCAGAAGCCTGATGATGCAGCTCGACGAGCCGCCGCAAC
TGGCGCTCGTCGACCTCGGCCTGCCGCCGCAGCCGCACAAGCCCGACGAGGGCTTCGCCCTGATCGCCGATCTGCTCGGC
ATTTCCCCGAGCATCAAGATCCTGGTCCTGTCGGGCCAGAGCGACGAGGCCAACGCGCGTCATGCGCGGGCGCTCGGCGC
GATCGACTTCGTCGCCAAGCCCTGCGAGCCGGAACGCCTGAAGTCGCTGCTGTTCAACGCGCTGCTCGTGCAGGACGCCG
AGCGCAGCGCCGACAAGGCGCCGGCGCCGGCCAAGGACTCCGGGATCGTCGGGCAGAGCCCGGCCATGGACAAGCTGCGC
GAACAGATCAAGCAATACGCCGCAGCGCCGTTCCCGGTGCTGATCGAAGGCGAATCGGGCAGCGGCAAGGAGCGCGTCGC
GTCGAGTCTGCATCAGCTCTCGCCGCGCTCGGCCAAGCCCTACCTCGCGCTCAACTGTGCCGCGATCTCGCCGACGCTGG
TCGAACCGACGCTGTTCGGTTACGCCAAGGGCGCGTTCACCGGCGCGACCAGCGCCCGGGCGGGCTACTTCGAGGAGGCC
GAGAACGGCACGCTGTTCCTCGACGAAATCGGCGAATTGCCGCTCGAACTGCAGGCCAAGCTCCTGCGCGTGCTCGAAAA
TGGCGAATACCAGCGCGTCGGCGAAACCCAGCCGCGCTTCTCGAACGCCCGCGTGGTCACCGCGACCAACCGCGACTTGC
GCGCCGAGATCAAGGCCGGGCGCTTCCGCGCCGACCTCTACCACCGTCTGTCGGTATTTTCGATCACCGTGCCGCCGCTG
CGAGAAATGGGCCAGGACAAGCTCACGCTGCTCGAGCACTTCCGTGATTTCTATGCGCGCGAAGCCAAGAGCGCACCCTT
CCAGCTCGATTCGCGTGCCCAGCAGATCTGGAAGGACTATCACTTCCCCGGCAACGTTCGCGAACTGCGCAACATCGCGA
TCCGCCTGACGACGAAGTATCCGGGAACGACGGTCAACGTCGAGCAGCTCGAAGCCGAACTCGACACCGATCAGGCCTAC
CCCCAGGAAATCCCGCTGGCGAGCGACAGCAAGGCGCTGATCGAACTCGCGCGCAAGCAGCTGCAGACGCTCGCCAACTT
CAACCTCGACGTCACGTTGCGCCAGTGGGAAAAAGCCTACGTCGAAGCCGCGCTCAACATGACGCACGGCAACCTGTCGC
AGGCAGCGAAGCTGCTCGGCATCAACCGCACCACGCTCTACAGCCGCATGCAGACCTACGGAAGCGAATAG

Upstream 100 bases:

>100_bases
TTGCCTCTCTGGCACAATCGTCGAAAGTCCGACAGCGCACAAAAAACGTCCTGCCCGCCGAGAGCAGACGCATGGTCGGC
ACGACTCGACCGGAGGATAT

Downstream 100 bases:

>100_bases
CCCTCGTCCGTGTATCTCGACTACTTCGGCCTGAAAGAAGCGCCCTTCCGCATCACGCCGAACACCGAGTACTGGTACGC
CGGCGGCCAGCGTGGAGAAA

Product: two component Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MVQTNKPTLLIVDDDPLISDTLTYVLSKDFEISAAESRAQVRSLMMQLDEPPQLALVDLGLPPQPHKPDEGFALIADLLG
ISPSIKILVLSGQSDEANARHARALGAIDFVAKPCEPERLKSLLFNALLVQDAERSADKAPAPAKDSGIVGQSPAMDKLR
EQIKQYAAAPFPVLIEGESGSGKERVASSLHQLSPRSAKPYLALNCAAISPTLVEPTLFGYAKGAFTGATSARAGYFEEA
ENGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQPRFSNARVVTATNRDLRAEIKAGRFRADLYHRLSVFSITVPPL
REMGQDKLTLLEHFRDFYAREAKSAPFQLDSRAQQIWKDYHFPGNVRELRNIAIRLTTKYPGTTVNVEQLEAELDTDQAY
PQEIPLASDSKALIELARKQLQTLANFNLDVTLRQWEKAYVEAALNMTHGNLSQAAKLLGINRTTLYSRMQTYGSE

Sequences:

>Translated_476_residues
MVQTNKPTLLIVDDDPLISDTLTYVLSKDFEISAAESRAQVRSLMMQLDEPPQLALVDLGLPPQPHKPDEGFALIADLLG
ISPSIKILVLSGQSDEANARHARALGAIDFVAKPCEPERLKSLLFNALLVQDAERSADKAPAPAKDSGIVGQSPAMDKLR
EQIKQYAAAPFPVLIEGESGSGKERVASSLHQLSPRSAKPYLALNCAAISPTLVEPTLFGYAKGAFTGATSARAGYFEEA
ENGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQPRFSNARVVTATNRDLRAEIKAGRFRADLYHRLSVFSITVPPL
REMGQDKLTLLEHFRDFYAREAKSAPFQLDSRAQQIWKDYHFPGNVRELRNIAIRLTTKYPGTTVNVEQLEAELDTDQAY
PQEIPLASDSKALIELARKQLQTLANFNLDVTLRQWEKAYVEAALNMTHGNLSQAAKLLGINRTTLYSRMQTYGSE
>Mature_476_residues
MVQTNKPTLLIVDDDPLISDTLTYVLSKDFEISAAESRAQVRSLMMQLDEPPQLALVDLGLPPQPHKPDEGFALIADLLG
ISPSIKILVLSGQSDEANARHARALGAIDFVAKPCEPERLKSLLFNALLVQDAERSADKAPAPAKDSGIVGQSPAMDKLR
EQIKQYAAAPFPVLIEGESGSGKERVASSLHQLSPRSAKPYLALNCAAISPTLVEPTLFGYAKGAFTGATSARAGYFEEA
ENGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQPRFSNARVVTATNRDLRAEIKAGRFRADLYHRLSVFSITVPPL
REMGQDKLTLLEHFRDFYAREAKSAPFQLDSRAQQIWKDYHFPGNVRELRNIAIRLTTKYPGTTVNVEQLEAELDTDQAY
PQEIPLASDSKALIELARKQLQTLANFNLDVTLRQWEKAYVEAALNMTHGNLSQAAKLLGINRTTLYSRMQTYGSE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=33.6842105263158, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=35.85313174946, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788550, Length=473, Percent_Identity=32.5581395348837, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI87082152, Length=340, Percent_Identity=42.0588235294118, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788905, Length=478, Percent_Identity=35.3556485355649, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI87082117, Length=329, Percent_Identity=41.0334346504559, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1789233, Length=344, Percent_Identity=37.7906976744186, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=356, Percent_Identity=39.8876404494382, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=38.3435582822086, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789087, Length=330, Percent_Identity=38.1818181818182, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787583, Length=328, Percent_Identity=35.3658536585366, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=31.9391634980989, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=29.5819935691318, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52615; Mature: 52615

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQTNKPTLLIVDDDPLISDTLTYVLSKDFEISAAESRAQVRSLMMQLDEPPQLALVDLG
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECC
LPPQPHKPDEGFALIADLLGISPSIKILVLSGQSDEANARHARALGAIDFVAKPCEPERL
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHH
KSLLFNALLVQDAERSADKAPAPAKDSGIVGQSPAMDKLREQIKQYAAAPFPVLIEGESG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
SGKERVASSLHQLSPRSAKPYLALNCAAISPTLVEPTLFGYAKGAFTGATSARAGYFEEA
CCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCHHCC
ENGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQPRFSNARVVTATNRDLRAEIKAG
CCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHC
RFRADLYHRLSVFSITVPPLREMGQDKLTLLEHFRDFYAREAKSAPFQLDSRAQQIWKDY
HHHHHHHHHHEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
HFPGNVRELRNIAIRLTTKYPGTTVNVEQLEAELDTDQAYPQEIPLASDSKALIELARKQ
CCCCCHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LQTLANFNLDVTLRQWEKAYVEAALNMTHGNLSQAAKLLGINRTTLYSRMQTYGSE
HHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MVQTNKPTLLIVDDDPLISDTLTYVLSKDFEISAAESRAQVRSLMMQLDEPPQLALVDLG
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCCEEEEECC
LPPQPHKPDEGFALIADLLGISPSIKILVLSGQSDEANARHARALGAIDFVAKPCEPERL
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHH
KSLLFNALLVQDAERSADKAPAPAKDSGIVGQSPAMDKLREQIKQYAAAPFPVLIEGESG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
SGKERVASSLHQLSPRSAKPYLALNCAAISPTLVEPTLFGYAKGAFTGATSARAGYFEEA
CCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCHHCC
ENGTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQPRFSNARVVTATNRDLRAEIKAG
CCCEEEHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHC
RFRADLYHRLSVFSITVPPLREMGQDKLTLLEHFRDFYAREAKSAPFQLDSRAQQIWKDY
HHHHHHHHHHEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
HFPGNVRELRNIAIRLTTKYPGTTVNVEQLEAELDTDQAYPQEIPLASDSKALIELARKQ
CCCCCHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LQTLANFNLDVTLRQWEKAYVEAALNMTHGNLSQAAKLLGINRTTLYSRMQTYGSE
HHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]