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The map label for this gene is bioD [H]

Identifier: 74316341

GI number: 74316341

Start: 346724

End: 347407

Strand: Direct

Name: bioD [H]

Synonym: Tbd_0323

Alternate gene names: 74316341

Gene position: 346724-347407 (Clockwise)

Preceding gene: 74316340

Following gene: 74316342

Centisome position: 11.92

GC content: 70.32

Gene sequence:

>684_bases
ATGAGCGCGCGCGGCCTGTTCGTCACAGGGACCGACACCGGCGTCGGCAAGACCCGCGTGGCGGTTGCCCTGATTCACGC
GCTGCGCGCGCAAGGACTGAACGTCGCGGCGATGAAGCCGGTGGCCGCGGGCTGCGCGCCGGGCGCGTTGAACGAGGATG
TGAGCGCGCTGCTGCAGGCTGCCAACGTCGCGGCCGAGCTGCGCGACGTCAACCCCTACAGCTTCGAGCCGGCGATCGCG
CCGCACATCGCGGCCGAGCTCGCCGGTGTGACGATCGACCTCGACGTGATCGCCGCCGCCTACGGCAGGCTCGCCGCGGC
CGCCGACGTCGTCGTCGTCGAGGGGGCCGGGGGCTGGCGCGTGCCACTCAACGCCAGCGAGGACATGGCGACGCTTGCCC
AAAAGCTCGGTCTGGCTGCCGTGCTGGTCGTCGGCCTGCGCCTCGGCTGCGTCAACCACGCCGTGCTGAGCGCGGCGTCG
ATCGTGTCGGCCGGGGTGCCCTGGACCGGGTGGATCGCGAATCGAATCAAACCGGCGATGCCCTACGAGGCGCAGAATGT
CGCTGCCTTGCAGGCGCGCCTGCCGGGTCCCTTCCTCGGCGTCCAGCCGTTTCTCACGGTGCCGACAGCAGACCAGGGCG
TGCCGCAATGGCTGACGCCCCCGACTATCAGTATCTTAGAATAG

Upstream 100 bases:

>100_bases
TCTACGGCCACGCGTGGGCCGGCGACAAGACCCACCTCGAGGACGGGCGCCAGGTCATCCAGTTCGCGATCGGCGAGCGC
CTGCGCAAGCGCGGTCGCGC

Downstream 100 bases:

>100_bases
TCTTTAATAATTATAATTAAATCAACGACTTAAATCGTATTTGAGACTTGTTCGCGGTCGCGGCACCGTGTTACTGTTCG
CGACAAAATGCCGCACCCGG

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSARGLFVTGTDTGVGKTRVAVALIHALRAQGLNVAAMKPVAAGCAPGALNEDVSALLQAANVAAELRDVNPYSFEPAIA
PHIAAELAGVTIDLDVIAAAYGRLAAAADVVVVEGAGGWRVPLNASEDMATLAQKLGLAAVLVVGLRLGCVNHAVLSAAS
IVSAGVPWTGWIANRIKPAMPYEAQNVAALQARLPGPFLGVQPFLTVPTADQGVPQWLTPPTISILE

Sequences:

>Translated_227_residues
MSARGLFVTGTDTGVGKTRVAVALIHALRAQGLNVAAMKPVAAGCAPGALNEDVSALLQAANVAAELRDVNPYSFEPAIA
PHIAAELAGVTIDLDVIAAAYGRLAAAADVVVVEGAGGWRVPLNASEDMATLAQKLGLAAVLVVGLRLGCVNHAVLSAAS
IVSAGVPWTGWIANRIKPAMPYEAQNVAALQARLPGPFLGVQPFLTVPTADQGVPQWLTPPTISILE
>Mature_226_residues
SARGLFVTGTDTGVGKTRVAVALIHALRAQGLNVAAMKPVAAGCAPGALNEDVSALLQAANVAAELRDVNPYSFEPAIAP
HIAAELAGVTIDLDVIAAAYGRLAAAADVVVVEGAGGWRVPLNASEDMATLAQKLGLAAVLVVGLRLGCVNHAVLSAASI
VSAGVPWTGWIANRIKPAMPYEAQNVAALQARLPGPFLGVQPFLTVPTADQGVPQWLTPPTISILE

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=208, Percent_Identity=40.8653846153846, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI87081944, Length=212, Percent_Identity=36.7924528301887, Blast_Score=134, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23133; Mature: 23002

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARGLFVTGTDTGVGKTRVAVALIHALRAQGLNVAAMKPVAAGCAPGALNEDVSALLQA
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCHHHHHHHHH
ANVAAELRDVNPYSFEPAIAPHIAAELAGVTIDLDVIAAAYGRLAAAADVVVVEGAGGWR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEHHHHHHHHHHHHHHCCEEEEECCCCEE
VPLNASEDMATLAQKLGLAAVLVVGLRLGCVNHAVLSAASIVSAGVPWTGWIANRIKPAM
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
PYEAQNVAALQARLPGPFLGVQPFLTVPTADQGVPQWLTPPTISILE
CCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
SARGLFVTGTDTGVGKTRVAVALIHALRAQGLNVAAMKPVAAGCAPGALNEDVSALLQA
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCHHHHHHHHH
ANVAAELRDVNPYSFEPAIAPHIAAELAGVTIDLDVIAAAYGRLAAAADVVVVEGAGGWR
HHHHHHHHCCCCCCCCCCHHHHHHHHHHCCEEEHHHHHHHHHHHHHHCCEEEEECCCCEE
VPLNASEDMATLAQKLGLAAVLVVGLRLGCVNHAVLSAASIVSAGVPWTGWIANRIKPAM
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCC
PYEAQNVAALQARLPGPFLGVQPFLTVPTADQGVPQWLTPPTISILE
CCCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA