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The map label for this gene is gatB

Identifier: 74316275

GI number: 74316275

Start: 277945

End: 279372

Strand: Reverse

Name: gatB

Synonym: Tbd_0257

Alternate gene names: 74316275

Gene position: 279372-277945 (Counterclockwise)

Preceding gene: 74316276

Following gene: 74316272

Centisome position: 9.6

GC content: 65.83

Gene sequence:

>1428_bases
ATGAAGTGGGAGACCGTCATCGGGCTCGAAGTGCATACCCAGCTCGCCACCCAGTCCAAGATCTTCTCGGCCAGCAGCAC
GGCCTTCGGCGCCGCGCCCAACACCCAGGCCAGCGCCGTCGACATCGCACTGCCCGGCGTGCTTCCCGTGCTCAACAAGG
CGGCGGTCGAATGCGCGATCAAGTTCGGCCTCGCGATCGGCGCGACGCTCAACCGCGTCAACGTGTTCGACCGCAAGAAC
TACTTCTACCCCGATCTTCCCAAGGGCTACCAGATCAGCCAGCTCGCCAAGCCCATCGTCGAGGGCGGCGCGCTCACGAT
CGTCGTCGACGGCGTCGAGAAGACGATTCATCTCACGCGCGCGCACATGGAGGAGGACGCCGGCAAGTCGCTGCACGAGG
ACTTCCACGGCATGACCGGCATCGATCTCAACCGCGCCGGCACGCCGCTGCTGGAGATCGTCTCCGAACCCGAGATGCGT
TCGTCGGCCGAAGCCGTCGCCTACGCGCGCGCGCTGCACACGCTCGTGACCTGGATCGGCATCTGCGACGGCAACATGCA
GGAAGGCAGCTTCCGCGTCGACGCCAACGTCTCGGTGCGCCCGCTCGGGCAGGCCGAATTCGGCACCCGGCGCGAGATCA
AGAACCTCAATTCGTTCCGCTTCCTGCAGCAGGCGATCGACTACGAAGTGCGCTGGCAGATCGAGACGCTCGAAGACGGC
GGCCGCATCCAGCAGGCCACGGTGCTGTTCGACCCCGACACCGGCGAGACGCGCATGATGCGCAGCAAGGAAGAGGCGCA
CGACTACCGCTACTTCCCCGATCCCGATCTGCTGCCCGTGAAGCTCGACGAGGCGTGGATCGACGCCGTGCGGACGACCC
TACCCGAGCTGCCGGCCGCGATGCGGACGCGCTTCCAGCAAGACTACGGCGTCTCGGCCTACGACGCCTCGGTGCTGACC
GGCTCGCGCGCGCTTGCCGCCTACTTCGAGGCTGCGGCCCAGCAATCCGGGCAGCCCAAGCTCGCCGCTAACTGGGTCAT
GGGCGAACTGTCCGCCGCGCTCAACAAGGCCGAGCTCGACATCGGCGAGAGCCCGGTTTCGGCCGTGCAACTCGGCACGC
TCATCACCCGGATTCAGGACGGCACGCTCTCCGGCAAGCTCGCCAAGCAGGTGTTCGAAGGGCTGTGGGAAGGCGCCGGC
GACGTCGACGGAATCATCGCGGCACGCGGGCTCAAGCAGATGTCCGACGCAGGTGAACTCGAGAAGATCGTCGACGAGGT
ACTCGCCGCCAATCAAAAGTCGGTCGAGGAATTCCGTGCGGGCAAGGACAAGGCGTTCAACGCGTTGGTCGGCCAGGTCA
TGAAGGCGAGCCGCGGCAAGGCCAATCCCGCACAGGTCAACGAACTGCTGAAAGCCAAGCTGCAGTAG

Upstream 100 bases:

>100_bases
AGCTCGTCGGCGATTATTTTTCCGAAGCGAAGATGCTGAACGTCGCCCATCAGTACCAGCGCGTCACCGACTGGCACGCG
CGCCAACCGGAGGCGCTCGC

Downstream 100 bases:

>100_bases
GGGACGACCCGGCGAGCTGCGCGCTTGCCGCGGGCGCGAAGGCGCCGCGCCTTAGGGCTGGCCCGTCAGCTCGCGAAAAC
GGGCCTTGTCGGCGGCGTAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKWETVIGLEVHTQLATQSKIFSASSTAFGAAPNTQASAVDIALPGVLPVLNKAAVECAIKFGLAIGATLNRVNVFDRKN
YFYPDLPKGYQISQLAKPIVEGGALTIVVDGVEKTIHLTRAHMEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPEMR
SSAEAVAYARALHTLVTWIGICDGNMQEGSFRVDANVSVRPLGQAEFGTRREIKNLNSFRFLQQAIDYEVRWQIETLEDG
GRIQQATVLFDPDTGETRMMRSKEEAHDYRYFPDPDLLPVKLDEAWIDAVRTTLPELPAAMRTRFQQDYGVSAYDASVLT
GSRALAAYFEAAAQQSGQPKLAANWVMGELSAALNKAELDIGESPVSAVQLGTLITRIQDGTLSGKLAKQVFEGLWEGAG
DVDGIIAARGLKQMSDAGELEKIVDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASRGKANPAQVNELLKAKLQ

Sequences:

>Translated_475_residues
MKWETVIGLEVHTQLATQSKIFSASSTAFGAAPNTQASAVDIALPGVLPVLNKAAVECAIKFGLAIGATLNRVNVFDRKN
YFYPDLPKGYQISQLAKPIVEGGALTIVVDGVEKTIHLTRAHMEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPEMR
SSAEAVAYARALHTLVTWIGICDGNMQEGSFRVDANVSVRPLGQAEFGTRREIKNLNSFRFLQQAIDYEVRWQIETLEDG
GRIQQATVLFDPDTGETRMMRSKEEAHDYRYFPDPDLLPVKLDEAWIDAVRTTLPELPAAMRTRFQQDYGVSAYDASVLT
GSRALAAYFEAAAQQSGQPKLAANWVMGELSAALNKAELDIGESPVSAVQLGTLITRIQDGTLSGKLAKQVFEGLWEGAG
DVDGIIAARGLKQMSDAGELEKIVDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASRGKANPAQVNELLKAKLQ
>Mature_475_residues
MKWETVIGLEVHTQLATQSKIFSASSTAFGAAPNTQASAVDIALPGVLPVLNKAAVECAIKFGLAIGATLNRVNVFDRKN
YFYPDLPKGYQISQLAKPIVEGGALTIVVDGVEKTIHLTRAHMEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPEMR
SSAEAVAYARALHTLVTWIGICDGNMQEGSFRVDANVSVRPLGQAEFGTRREIKNLNSFRFLQQAIDYEVRWQIETLEDG
GRIQQATVLFDPDTGETRMMRSKEEAHDYRYFPDPDLLPVKLDEAWIDAVRTTLPELPAAMRTRFQQDYGVSAYDASVLT
GSRALAAYFEAAAQQSGQPKLAANWVMGELSAALNKAELDIGESPVSAVQLGTLITRIQDGTLSGKLAKQVFEGLWEGAG
DVDGIIAARGLKQMSDAGELEKIVDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASRGKANPAQVNELLKAKLQ

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=40, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=474, Percent_Identity=31.8565400843882, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=32.2709163346614, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45550808, Length=479, Percent_Identity=35.9081419624217, Blast_Score=312, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_THIDA (Q3SM40)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314015.1
- ProteinModelPortal:   Q3SM40
- SMR:   Q3SM40
- STRING:   Q3SM40
- PRIDE:   Q3SM40
- GeneID:   3671399
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0257
- NMPDR:   fig|292415.3.peg.364
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q3SM40
- ProtClustDB:   PRK05477
- BioCyc:   TDEN292415:TBD_0257-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 51650; Mature: 51650

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWETVIGLEVHTQLATQSKIFSASSTAFGAAPNTQASAVDIALPGVLPVLNKAAVECAI
CCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
KFGLAIGATLNRVNVFDRKNYFYPDLPKGYQISQLAKPIVEGGALTIVVDGVEKTIHLTR
HHHHHHHCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHH
AHMEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPEMRSSAEAVAYARALHTLVTWIG
HHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMQEGSFRVDANVSVRPLGQAEFGTRREIKNLNSFRFLQQAIDYEVRWQIETLEDG
HCCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHCCHHHHHHHHHCCEEEEEEEEEECCC
GRIQQATVLFDPDTGETRMMRSKEEAHDYRYFPDPDLLPVKLDEAWIDAVRTTLPELPAA
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHH
MRTRFQQDYGVSAYDASVLTGSRALAAYFEAAAQQSGQPKLAANWVMGELSAALNKAELD
HHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
IGESPVSAVQLGTLITRIQDGTLSGKLAKQVFEGLWEGAGDVDGIIAARGLKQMSDAGEL
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHH
EKIVDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASRGKANPAQVNELLKAKLQ
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKWETVIGLEVHTQLATQSKIFSASSTAFGAAPNTQASAVDIALPGVLPVLNKAAVECAI
CCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
KFGLAIGATLNRVNVFDRKNYFYPDLPKGYQISQLAKPIVEGGALTIVVDGVEKTIHLTR
HHHHHHHCHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHH
AHMEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVSEPEMRSSAEAVAYARALHTLVTWIG
HHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ICDGNMQEGSFRVDANVSVRPLGQAEFGTRREIKNLNSFRFLQQAIDYEVRWQIETLEDG
HCCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHCCHHHHHHHHHCCEEEEEEEEEECCC
GRIQQATVLFDPDTGETRMMRSKEEAHDYRYFPDPDLLPVKLDEAWIDAVRTTLPELPAA
CCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCHH
MRTRFQQDYGVSAYDASVLTGSRALAAYFEAAAQQSGQPKLAANWVMGELSAALNKAELD
HHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
IGESPVSAVQLGTLITRIQDGTLSGKLAKQVFEGLWEGAGDVDGIIAARGLKQMSDAGEL
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHH
EKIVDEVLAANQKSVEEFRAGKDKAFNALVGQVMKASRGKANPAQVNELLKAKLQ
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA