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The map label for this gene is gatA

Identifier: 74316276

GI number: 74316276

Start: 279369

End: 280826

Strand: Reverse

Name: gatA

Synonym: Tbd_0258

Alternate gene names: 74316276

Gene position: 280826-279369 (Counterclockwise)

Preceding gene: 74316277

Following gene: 74316275

Centisome position: 9.65

GC content: 67.22

Gene sequence:

>1458_bases
ATGCCCGCACCCTCCCTTTCCGCCCTCGCTGCACAACTCGCGGCGAAACAGGTGTCGAGCCGCGAGCTCGCGCAGCAATA
TCTCGACCGCATCGCCGCGCTGAACCCGACGATCAACGCCTTCATTACGGTCGACCCGGAGAAGACGCTCGCCGAGGCGG
CGGCTGCCGACGCGCTGATCGCCGGCGGCGCCGCCGGACCGCTGACTGGCGTGCCGATCGCGCACAAGGACATCTTCTGC
ACCGACGGCTGGCTCACGACCTGCGGCTCGAAAATGCTCGCGAACTTCGTCGCGCCCTACGACGCCCACGTCATCGAGCG
CTGCAAGGCCGCCGGCATGCCGAGCCTCGGCAAGACCAACATGGACGAGTTCGCCATGGGCTCGTCGAACGAAACCTCGT
TCTTCGGCGCGGTGAAGAACCCGTGGAACGCCGGCTACGTGCCCGGCGGCTCGTCGGGCGGCGCCGCGGCCTGCGTCGCT
GCACGGATGGCACCCGCCGCGACCGGCACCGACACCGGCGGCTCGATCCGCCAGCCGGCCGCGCTGTGCGGCATCACCGG
GCTCAAGCCGACCTACGGCCTGGTCTCGCGCTACGGCATGATCGCCTTCGCCTCGAGTCTCGACCAGGCGGGCCCGATGG
CGCCGTCGGCCGAAGACTGTGCGCGGCTTTTGAACGTGATAACGGGCTTCGATCCGAAGGACTCGACCAGCCTCGAGCGC
GAAAAGGAAGACTACACGCGCGACCTCGATCTCCCGCTCACGGGCCTACGCGTCGGCCTGCCGTGCGAATTTTTTGCCGA
GGGTCTCAACAACGAGGTCGCCGCTGCGGTCGACGCCGCGGTCAATGAACTGAAGAAGCTCGGCGCGACGACGGTCGAGA
TCTCGCTCGCCAACTCCCGGCTCGCGATCCCCGTCTACTACGTGCTCGCGCCCGCCGAGGCGTCGTCCAACCTCTCGCGC
TTCGACGGCGTGCGCTACGGCTACCGCGCCCCCGAGTACGCGGACCTCAACGACATGTACGCCAAGACCCGTGCGCAGGG
TTTCGGCGCGGAGGTCAAGCGCCGCATCATGATCGGCACCTACGTGCTGAGCCACGGCTACTACGACGCCTACTACCTGC
AGGCGCAAAAAATTCGCCGCCTGATCGCGCACGACTTCGCCGAGGCATTCAAGACCTGCGACGTCATCCTCGGCCCGACC
GCGCCGGGCACGGCATTCAAGCTCGGCGAGAAATCCGACGACCCGGTCGAGATGTACCTCAACGACCTCTACACGATTCC
GGCCAACCTCGCGGGCCTGCCCGGCATGAGCCTGCCCTGCGGCTTCGATTCGCAGGGCCTGCCCATCGGCCTGCAGCTCG
TCGGCGATTATTTTTCCGAAGCGAAGATGCTGAACGTCGCCCATCAGTACCAGCGCGTCACCGACTGGCACGCGCGCCAA
CCGGAGGCGCTCGCATGA

Upstream 100 bases:

>100_bases
ATTCCAGGCCGTCGCACCGGCGGTCGAAAACGGCCTGTATCTCGTGCCGAAAGTGATCGAGTGAGCGTCTCCCATTCCGC
CGTGCCGAATCCGTAACGCC

Downstream 100 bases:

>100_bases
AGTGGGAGACCGTCATCGGGCTCGAAGTGCATACCCAGCTCGCCACCCAGTCCAAGATCTTCTCGGCCAGCAGCACGGCC
TTCGGCGCCGCGCCCAACAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPAPSLSALAAQLAAKQVSSRELAQQYLDRIAALNPTINAFITVDPEKTLAEAAAADALIAGGAAGPLTGVPIAHKDIFC
TDGWLTTCGSKMLANFVAPYDAHVIERCKAAGMPSLGKTNMDEFAMGSSNETSFFGAVKNPWNAGYVPGGSSGGAAACVA
ARMAPAATGTDTGGSIRQPAALCGITGLKPTYGLVSRYGMIAFASSLDQAGPMAPSAEDCARLLNVITGFDPKDSTSLER
EKEDYTRDLDLPLTGLRVGLPCEFFAEGLNNEVAAAVDAAVNELKKLGATTVEISLANSRLAIPVYYVLAPAEASSNLSR
FDGVRYGYRAPEYADLNDMYAKTRAQGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRLIAHDFAEAFKTCDVILGPT
APGTAFKLGEKSDDPVEMYLNDLYTIPANLAGLPGMSLPCGFDSQGLPIGLQLVGDYFSEAKMLNVAHQYQRVTDWHARQ
PEALA

Sequences:

>Translated_485_residues
MPAPSLSALAAQLAAKQVSSRELAQQYLDRIAALNPTINAFITVDPEKTLAEAAAADALIAGGAAGPLTGVPIAHKDIFC
TDGWLTTCGSKMLANFVAPYDAHVIERCKAAGMPSLGKTNMDEFAMGSSNETSFFGAVKNPWNAGYVPGGSSGGAAACVA
ARMAPAATGTDTGGSIRQPAALCGITGLKPTYGLVSRYGMIAFASSLDQAGPMAPSAEDCARLLNVITGFDPKDSTSLER
EKEDYTRDLDLPLTGLRVGLPCEFFAEGLNNEVAAAVDAAVNELKKLGATTVEISLANSRLAIPVYYVLAPAEASSNLSR
FDGVRYGYRAPEYADLNDMYAKTRAQGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRLIAHDFAEAFKTCDVILGPT
APGTAFKLGEKSDDPVEMYLNDLYTIPANLAGLPGMSLPCGFDSQGLPIGLQLVGDYFSEAKMLNVAHQYQRVTDWHARQ
PEALA
>Mature_484_residues
PAPSLSALAAQLAAKQVSSRELAQQYLDRIAALNPTINAFITVDPEKTLAEAAAADALIAGGAAGPLTGVPIAHKDIFCT
DGWLTTCGSKMLANFVAPYDAHVIERCKAAGMPSLGKTNMDEFAMGSSNETSFFGAVKNPWNAGYVPGGSSGGAAACVAA
RMAPAATGTDTGGSIRQPAALCGITGLKPTYGLVSRYGMIAFASSLDQAGPMAPSAEDCARLLNVITGFDPKDSTSLERE
KEDYTRDLDLPLTGLRVGLPCEFFAEGLNNEVAAAVDAAVNELKKLGATTVEISLANSRLAIPVYYVLAPAEASSNLSRF
DGVRYGYRAPEYADLNDMYAKTRAQGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRLIAHDFAEAFKTCDVILGPTA
PGTAFKLGEKSDDPVEMYLNDLYTIPANLAGLPGMSLPCGFDSQGLPIGLQLVGDYFSEAKMLNVAHQYQRVTDWHARQP
EALA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=512, Percent_Identity=36.5234375, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI195972892, Length=486, Percent_Identity=25.9259259259259, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI166795287, Length=215, Percent_Identity=31.1627906976744, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=39.8633257403189, Blast_Score=295, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=484, Percent_Identity=25.8264462809917, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=184, Percent_Identity=30.4347826086957, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=188, Percent_Identity=28.7234042553192, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71990152, Length=164, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=186, Percent_Identity=30.1075268817204, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=186, Percent_Identity=30.1075268817204, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=34.7639484978541, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=469, Percent_Identity=28.7846481876333, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648113, Length=485, Percent_Identity=40, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=26.369168356998, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=26.369168356998, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=26.369168356998, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=26.369168356998, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=26.1386138613861, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=28.0392156862745, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648435, Length=494, Percent_Identity=25.7085020242915, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648437, Length=494, Percent_Identity=25.7085020242915, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550774, Length=499, Percent_Identity=25.6513026052104, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21356731, Length=486, Percent_Identity=24.8971193415638, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648441, Length=431, Percent_Identity=24.5939675174014, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648439, Length=431, Percent_Identity=24.5939675174014, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_THIDA (Q3SM39)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314016.1
- ProteinModelPortal:   Q3SM39
- SMR:   Q3SM39
- STRING:   Q3SM39
- GeneID:   3671400
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0258
- NMPDR:   fig|292415.3.peg.365
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   ICTVIAN
- PhylomeDB:   Q3SM39
- ProtClustDB:   PRK00012
- BioCyc:   TDEN292415:TBD_0258-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51432; Mature: 51301

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPSLSALAAQLAAKQVSSRELAQQYLDRIAALNPTINAFITVDPEKTLAEAAAADALI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
AGGAAGPLTGVPIAHKDIFCTDGWLTTCGSKMLANFVAPYDAHVIERCKAAGMPSLGKTN
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
MDEFAMGSSNETSFFGAVKNPWNAGYVPGGSSGGAAACVAARMAPAATGTDTGGSIRQPA
HHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCH
ALCGITGLKPTYGLVSRYGMIAFASSLDQAGPMAPSAEDCARLLNVITGFDPKDSTSLER
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
EKEDYTRDLDLPLTGLRVGLPCEFFAEGLNNEVAAAVDAAVNELKKLGATTVEISLANSR
HHHHHHCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCC
LAIPVYYVLAPAEASSNLSRFDGVRYGYRAPEYADLNDMYAKTRAQGFGAEVKRRIMIGT
EEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
YVLSHGYYDAYYLQAQKIRRLIAHDFAEAFKTCDVILGPTAPGTAFKLGEKSDDPVEMYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEECCCCCCCHHHHHH
NDLYTIPANLAGLPGMSLPCGFDSQGLPIGLQLVGDYFSEAKMLNVAHQYQRVTDWHARQ
HHHHHCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEALA
CCCCC
>Mature Secondary Structure 
PAPSLSALAAQLAAKQVSSRELAQQYLDRIAALNPTINAFITVDPEKTLAEAAAADALI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
AGGAAGPLTGVPIAHKDIFCTDGWLTTCGSKMLANFVAPYDAHVIERCKAAGMPSLGKTN
CCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
MDEFAMGSSNETSFFGAVKNPWNAGYVPGGSSGGAAACVAARMAPAATGTDTGGSIRQPA
HHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCH
ALCGITGLKPTYGLVSRYGMIAFASSLDQAGPMAPSAEDCARLLNVITGFDPKDSTSLER
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHH
EKEDYTRDLDLPLTGLRVGLPCEFFAEGLNNEVAAAVDAAVNELKKLGATTVEISLANSR
HHHHHHCCCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCC
LAIPVYYVLAPAEASSNLSRFDGVRYGYRAPEYADLNDMYAKTRAQGFGAEVKRRIMIGT
EEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
YVLSHGYYDAYYLQAQKIRRLIAHDFAEAFKTCDVILGPTAPGTAFKLGEKSDDPVEMYL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEECCCCCCCHHHHHH
NDLYTIPANLAGLPGMSLPCGFDSQGLPIGLQLVGDYFSEAKMLNVAHQYQRVTDWHARQ
HHHHHCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEALA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA