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The map label for this gene is exoA [H]

Identifier: 74316272

GI number: 74316272

Start: 275859

End: 276638

Strand: Reverse

Name: exoA [H]

Synonym: Tbd_0254

Alternate gene names: 74316272

Gene position: 276638-275859 (Counterclockwise)

Preceding gene: 74316275

Following gene: 74316271

Centisome position: 9.51

GC content: 63.59

Gene sequence:

>780_bases
ATGCGAATTATATCGGCCAACCTCAACGGCATCCGTTCGGCGGCCACCAAGGGCTTCTTCGACTGGCTGCCGACGCAGGG
CGCGGACGTCGTCTGCGTGCAGGAACTGAAAGCCCAGGCGACCGACCTCAAGCCGGAAGTGCGACAGTGCGACGGGCTCA
GCGGGGCCTTTCACTGCGCCGAGAAGAAAGGCTACAGCGGCGTCGGCGTCTACACGCGCCAGGCGCCGCAGCGCATCGTC
GAAGGTCTCGGCTTGCCCGAATTCGACGCCGAGGGCCGCTACATCGAAGCCGATTACGGAGATCTCGCGGTCATCTCGCT
CTATCAGCCTTCGGGCTCGAGCAGCGAAGAGCGGCAGCAGGCGAAATTCCGCTTCCTCGATGCCTTCTTTCCCCATCTGG
AGAAACTGGTCAAATGCGGCCGCGAGATCATCATCTGCGGCGACTGGAACATCGCCCACCAGGAAATCGACCTCAAGAAC
TGGAAATCCAACCAGAAGAACTCGGGCTTCCTGCCCGAGGAACGCGCCTGGATGACGCGCCTGTTCGACGAACTCGGCTG
GGTCGACGTCTACCGCAGCCTCTACCCCGCGCACACCGGCGAGGCCTACACCTGGTGGAGCAACCGCGGCCAGGCCTGGG
CCAAGAACGTCGGCTGGCGCATCGACTACCAGATCGCGACGCCGGGCATCGCGGCGAAGGCCCGCTCGGCTTTCGTGTAC
AAGGAACAGCGGTTTTCCGACCACGCCCCGCTGGTCGTCGATTACGACCATGCGCTGTAG

Upstream 100 bases:

>100_bases
TGAACTCGCCAAAACACAACACCTGCGAGGCGACGGCAAACTCGACGAACTCGGCTCTGTAATCGGACATTTCCGGACTT
TTCTTGAGTAGATAAACGCG

Downstream 100 bases:

>100_bases
CTGAACACAGACCGACGCAACCATGCCCAGCCCCTCCGCCAGCCACCCCTGGCTCGCCGCGCTCAAGGTCTACGCCCACC
CGCGTGTTGTCGGCATGCTG

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRIISANLNGIRSAATKGFFDWLPTQGADVVCVQELKAQATDLKPEVRQCDGLSGAFHCAEKKGYSGVGVYTRQAPQRIV
EGLGLPEFDAEGRYIEADYGDLAVISLYQPSGSSSEERQQAKFRFLDAFFPHLEKLVKCGREIIICGDWNIAHQEIDLKN
WKSNQKNSGFLPEERAWMTRLFDELGWVDVYRSLYPAHTGEAYTWWSNRGQAWAKNVGWRIDYQIATPGIAAKARSAFVY
KEQRFSDHAPLVVDYDHAL

Sequences:

>Translated_259_residues
MRIISANLNGIRSAATKGFFDWLPTQGADVVCVQELKAQATDLKPEVRQCDGLSGAFHCAEKKGYSGVGVYTRQAPQRIV
EGLGLPEFDAEGRYIEADYGDLAVISLYQPSGSSSEERQQAKFRFLDAFFPHLEKLVKCGREIIICGDWNIAHQEIDLKN
WKSNQKNSGFLPEERAWMTRLFDELGWVDVYRSLYPAHTGEAYTWWSNRGQAWAKNVGWRIDYQIATPGIAAKARSAFVY
KEQRFSDHAPLVVDYDHAL
>Mature_259_residues
MRIISANLNGIRSAATKGFFDWLPTQGADVVCVQELKAQATDLKPEVRQCDGLSGAFHCAEKKGYSGVGVYTRQAPQRIV
EGLGLPEFDAEGRYIEADYGDLAVISLYQPSGSSSEERQQAKFRFLDAFFPHLEKLVKCGREIIICGDWNIAHQEIDLKN
WKSNQKNSGFLPEERAWMTRLFDELGWVDVYRSLYPAHTGEAYTWWSNRGQAWAKNVGWRIDYQIATPGIAAKARSAFVY
KEQRFSDHAPLVVDYDHAL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=35.2941176470588, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=35.2941176470588, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=35.2941176470588, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=26.8370607028754, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=29.2134831460674, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=30.1158301158301, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=34.7826086956522, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=34.7826086956522, Blast_Score=148, Evalue=4e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29317; Mature: 29317

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISANLNGIRSAATKGFFDWLPTQGADVVCVQELKAQATDLKPEVRQCDGLSGAFHCA
CEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHHHHCCCCCHHHHHH
EKKGYSGVGVYTRQAPQRIVEGLGLPEFDAEGRYIEADYGDLAVISLYQPSGSSSEERQQ
HHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHH
AKFRFLDAFFPHLEKLVKCGREIIICGDWNIAHQEIDLKNWKSNQKNSGFLPEERAWMTR
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHEECHHHCCCCCCCCCCCCHHHHHHHH
LFDELGWVDVYRSLYPAHTGEAYTWWSNRGQAWAKNVGWRIDYQIATPGIAAKARSAFVY
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCEEEEEEEECCCHHHHHHHHHEE
KEQRFSDHAPLVVDYDHAL
HHHHCCCCCCEEEECCCCC
>Mature Secondary Structure
MRIISANLNGIRSAATKGFFDWLPTQGADVVCVQELKAQATDLKPEVRQCDGLSGAFHCA
CEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCHHHHHCCCCCHHHHHH
EKKGYSGVGVYTRQAPQRIVEGLGLPEFDAEGRYIEADYGDLAVISLYQPSGSSSEERQQ
HHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHHHHH
AKFRFLDAFFPHLEKLVKCGREIIICGDWNIAHQEIDLKNWKSNQKNSGFLPEERAWMTR
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHEECHHHCCCCCCCCCCCCHHHHHHHH
LFDELGWVDVYRSLYPAHTGEAYTWWSNRGQAWAKNVGWRIDYQIATPGIAAKARSAFVY
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCEEEEEEEECCCHHHHHHHHHEE
KEQRFSDHAPLVVDYDHAL
HHHHCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]