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The map label for this gene is argH

Identifier: 74316247

GI number: 74316247

Start: 250631

End: 252022

Strand: Reverse

Name: argH

Synonym: Tbd_0229

Alternate gene names: 74316247

Gene position: 252022-250631 (Counterclockwise)

Preceding gene: 74316252

Following gene: 74316244

Centisome position: 8.66

GC content: 67.39

Gene sequence:

>1392_bases
ATGAGCACGTCGTCGACGCCGCCGCAAGGCGCCTGGTCGGGCCGCTTTTCCGAGCCTGTTTCCGAAATCGTCAAACGCTA
CACGGCGTCGATTCCTTTTGATTATCGGCTCGCCGAGTTCGATATTCAGGGCTCGCTCGCGCACGCGGCGATGCTGCACC
ACGTCGGCGTCCTGAGCGCCGACGACCTCGCGGCGATCCGCGGCGGCATGGGCGAGCTGCTCGAAGAGATACGCGCCGGG
CGCTTCGCCTGGTCGCTGGATAAGGAGGATGTGCACCTCAACATCGAGGCGGCGCTGACGGCGAAGATCGGCGACGCCGG
CAAGCGCCTGCACACCGGACGCAGCCGCAACGATCAGGTCGCGACCGACATCCGGCTCTATCTGCGCGACGCGATCGACC
GCATCCTCGCGGGCCTCAAGGCGTGCCAGACCTCGCTCGTCGACCTTGCCGAGGCGCACGCCGACACCGTAATGCCGGGC
TTCACCCACCTGCAGGTCGCGCAGCCGGTGACCTTCGGCCACCATCTTCTCGCCTATTTCGAGATGCTCGCCCGCGACGC
CGAGCGCATGGCCGACTGCCGACGGCGCGTCAACCGCCTCCCGCTCGGCGCCGCGGCGCTCGCGGGCACGAGCTACCCCA
TCGATCGCGCCTACGTCGCGCGCGAACTCGGCTTCGAGGCCGTGTGCGAGAACTCGCTCGACGCCGTCTCCGACCGCGAC
TTCGCGATCGAATTCGCGGCCGCGGCGGCGCTCGTGATGACGCATCTGTCGCGCCTCTCGGAAGAACTGATCCTGTGGAT
GAGCCCGCGCTTCGGCTTCATCGACCTTGCCGACCGCTTCTGCACCGGCTCGTCGATCATGCCGCAGAAGAAGAATCCTG
ACGTGCCCGAACTCGTGCGCGGCAAGACCGGGCGGGTCAACGGCCACCTCGTCGCGTTGCTGACCCTGATGAAGGGCCAG
CCGCTCGCATACAACAAGGACAATCAGGAAGACAAGGAACCGCTATTCGATACGGTCGACACCCTGGCCGACACGCTTGC
GATCTACGCCGACATGCTGCGCGGCGTGACGGTCAAGCCCGACGCGATGCACGCGGCCGTGATGCAAGGCTTCGCGACCG
CCACCGACCTCGCCGACTACCTGGTCAAGAAAGGCCTGCCCTTCCGCGATGCGCACGAAGTCGTTGCGCGTGCCGTGCGC
GTCGCCGACGAATCCGGGCGCGACCTGGCCGACTTGCCGCTCGACGAACTGCAGACGTTCAGCCCGATCATCGGCGACGA
CGTCTACGCACTATTGACCCTCGAAGGCTCGCTCGCCGCCCGCGACCATTTCGGCGGCACCGCGCCCGCGCAGGTCCGCG
CAGCGGTCGCGCGCGCCCGCGGGCGACTTTAA

Upstream 100 bases:

>100_bases
GTCAGTGCGATCCGCAGGCTGACCCCGAGGCGGCAGAAGTTCGGCAGTTCGACCTCGCGGTTGTTATGATTCCTTCTTTT
CACTGTGCGTTTCTGTCCCC

Downstream 100 bases:

>100_bases
TCGGCTGGCGGCGGCGCGTGCTCGCGGCAGATCCGCCGCACCTCGGGGCTGCCGAGTTTTTCCTGCATCAGTTCGCAAAA
ATAGCTGCGCGGCCCGAGCC

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSTSSTPPQGAWSGRFSEPVSEIVKRYTASIPFDYRLAEFDIQGSLAHAAMLHHVGVLSADDLAAIRGGMGELLEEIRAG
RFAWSLDKEDVHLNIEAALTAKIGDAGKRLHTGRSRNDQVATDIRLYLRDAIDRILAGLKACQTSLVDLAEAHADTVMPG
FTHLQVAQPVTFGHHLLAYFEMLARDAERMADCRRRVNRLPLGAAALAGTSYPIDRAYVARELGFEAVCENSLDAVSDRD
FAIEFAAAAALVMTHLSRLSEELILWMSPRFGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLTLMKGQ
PLAYNKDNQEDKEPLFDTVDTLADTLAIYADMLRGVTVKPDAMHAAVMQGFATATDLADYLVKKGLPFRDAHEVVARAVR
VADESGRDLADLPLDELQTFSPIIGDDVYALLTLEGSLAARDHFGGTAPAQVRAAVARARGRL

Sequences:

>Translated_463_residues
MSTSSTPPQGAWSGRFSEPVSEIVKRYTASIPFDYRLAEFDIQGSLAHAAMLHHVGVLSADDLAAIRGGMGELLEEIRAG
RFAWSLDKEDVHLNIEAALTAKIGDAGKRLHTGRSRNDQVATDIRLYLRDAIDRILAGLKACQTSLVDLAEAHADTVMPG
FTHLQVAQPVTFGHHLLAYFEMLARDAERMADCRRRVNRLPLGAAALAGTSYPIDRAYVARELGFEAVCENSLDAVSDRD
FAIEFAAAAALVMTHLSRLSEELILWMSPRFGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLTLMKGQ
PLAYNKDNQEDKEPLFDTVDTLADTLAIYADMLRGVTVKPDAMHAAVMQGFATATDLADYLVKKGLPFRDAHEVVARAVR
VADESGRDLADLPLDELQTFSPIIGDDVYALLTLEGSLAARDHFGGTAPAQVRAAVARARGRL
>Mature_462_residues
STSSTPPQGAWSGRFSEPVSEIVKRYTASIPFDYRLAEFDIQGSLAHAAMLHHVGVLSADDLAAIRGGMGELLEEIRAGR
FAWSLDKEDVHLNIEAALTAKIGDAGKRLHTGRSRNDQVATDIRLYLRDAIDRILAGLKACQTSLVDLAEAHADTVMPGF
THLQVAQPVTFGHHLLAYFEMLARDAERMADCRRRVNRLPLGAAALAGTSYPIDRAYVARELGFEAVCENSLDAVSDRDF
AIEFAAAAALVMTHLSRLSEELILWMSPRFGFIDLADRFCTGSSIMPQKKNPDVPELVRGKTGRVNGHLVALLTLMKGQP
LAYNKDNQEDKEPLFDTVDTLADTLAIYADMLRGVTVKPDAMHAAVMQGFATATDLADYLVKKGLPFRDAHEVVARAVRV
ADESGRDLADLPLDELQTFSPIIGDDVYALLTLEGSLAARDHFGGTAPAQVRAAVARARGRL

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=452, Percent_Identity=42.6991150442478, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI68303542, Length=452, Percent_Identity=42.6991150442478, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI68303547, Length=452, Percent_Identity=40.4867256637168, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI68303549, Length=452, Percent_Identity=40.0442477876106, Blast_Score=327, Evalue=2e-89,
Organism=Escherichia coli, GI1790398, Length=453, Percent_Identity=50.7726269315673, Blast_Score=451, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321806, Length=462, Percent_Identity=41.991341991342, Blast_Score=365, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221473854, Length=448, Percent_Identity=41.7410714285714, Blast_Score=350, Evalue=9e-97,
Organism=Drosophila melanogaster, GI78706858, Length=448, Percent_Identity=41.7410714285714, Blast_Score=350, Evalue=9e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_THIDA (Q3SM68)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_313987.1
- ProteinModelPortal:   Q3SM68
- SMR:   Q3SM68
- STRING:   Q3SM68
- GeneID:   3671371
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0229
- NMPDR:   fig|292415.3.peg.257
- eggNOG:   COG0165
- HOGENOM:   HBG539632
- OMA:   MAEDLIF
- PhylomeDB:   Q3SM68
- ProtClustDB:   PRK00855
- BioCyc:   TDEN292415:TBD_0229-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 50434; Mature: 50303

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSTPPQGAWSGRFSEPVSEIVKRYTASIPFDYRLAEFDIQGSLAHAAMLHHVGVLSA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
DDLAAIRGGMGELLEEIRAGRFAWSLDKEDVHLNIEAALTAKIGDAGKRLHTGRSRNDQV
CHHHHHHCCHHHHHHHHHCCCEEEECCCCCEEEEEEEEEEEHHCHHHHHHHCCCCCCCHH
ATDIRLYLRDAIDRILAGLKACQTSLVDLAEAHADTVMPGFTHLQVAQPVTFGHHLLAYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
EMLARDAERMADCRRRVNRLPLGAAALAGTSYPIDRAYVARELGFEAVCENSLDAVSDRD
HHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHCHHHHHCCCHHHCCCCC
FAIEFAAAAALVMTHLSRLSEELILWMSPRFGFIDLADRFCTGSSIMPQKKNPDVPELVR
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHC
GKTGRVNGHLVALLTLMKGQPLAYNKDNQEDKEPLFDTVDTLADTLAIYADMLRGVTVKP
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DAMHAAVMQGFATATDLADYLVKKGLPFRDAHEVVARAVRVADESGRDLADLPLDELQTF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SPIIGDDVYALLTLEGSLAARDHFGGTAPAQVRAAVARARGRL
CCHHCCCEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STSSTPPQGAWSGRFSEPVSEIVKRYTASIPFDYRLAEFDIQGSLAHAAMLHHVGVLSA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCC
DDLAAIRGGMGELLEEIRAGRFAWSLDKEDVHLNIEAALTAKIGDAGKRLHTGRSRNDQV
CHHHHHHCCHHHHHHHHHCCCEEEECCCCCEEEEEEEEEEEHHCHHHHHHHCCCCCCCHH
ATDIRLYLRDAIDRILAGLKACQTSLVDLAEAHADTVMPGFTHLQVAQPVTFGHHLLAYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHH
EMLARDAERMADCRRRVNRLPLGAAALAGTSYPIDRAYVARELGFEAVCENSLDAVSDRD
HHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHCHHHHHCCCHHHCCCCC
FAIEFAAAAALVMTHLSRLSEELILWMSPRFGFIDLADRFCTGSSIMPQKKNPDVPELVR
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHC
GKTGRVNGHLVALLTLMKGQPLAYNKDNQEDKEPLFDTVDTLADTLAIYADMLRGVTVKP
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DAMHAAVMQGFATATDLADYLVKKGLPFRDAHEVVARAVRVADESGRDLADLPLDELQTF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SPIIGDDVYALLTLEGSLAARDHFGGTAPAQVRAAVARARGRL
CCHHCCCEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA