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The map label for this gene is pulE [H]

Identifier: 73542914

GI number: 73542914

Start: 3541955

End: 3543430

Strand: Direct

Name: pulE [H]

Synonym: Reut_A3230

Alternate gene names: 73542914

Gene position: 3541955-3543430 (Clockwise)

Preceding gene: 73542913

Following gene: 73542915

Centisome position: 93.05

GC content: 67.62

Gene sequence:

>1476_bases
ATGGCGGCGCGCCTGGTGTCGTACAGCTTTGCACGCGAAGCGCCGCTGCTGATCGCGCACCAGCGCGCCGACGGCCTTGA
AGTCTGGGTGAGCCGCGCCACATCGCCCGCTGCGCTTGCGGAGGTGGCGCGCGTGCACGGCGCGCTGCGCCTGCGCGTGA
TGGAGCCCGATGCGCTCGAACGCGCCATGTCCGACGCCTACAACCGCCAGGACGGCAGCGCCGCGCAGGTGGTGGGCGAA
GTCGAAGGCGAAGTCGACCTGTCGCGCCTGATGCAGGACATTCCCGCTGTGGAAGATCTGCTCGAGTCCGAAGACGACGC
GCCGATCATCCGCATGATCAACGCGCTGCTCACACAGGCCGCGCGCGAAGGCGCATCGGACATCCACATCGAACCGTTCG
AATCGGCGTCGGTCGTGCGCTTCCGTGTCGACGGCACGCTGCGCGACGTGGTGCGGCCGAAGAAGGCGCTGCACGGCGCG
CTGATCTCGCGCATCAAGATCATGGCGCAGCTCGATATTGCCGAGAAACGCTTGCCGCAGGACGGCCGGATCACGCTGCG
CGTGGGCGGGCGCCCCGTGGATGTGCGCGTGTCCACGCTGCCGACGGGCCACGGCGAGCGCGCGGTGCTGCGTCTGCTGG
ACAAGGAGGCAGGCCGCCTCGACCTGGCCAAGCTCGGCATGTCGCCGCGCACGCTGGCCGGTTTCGACGACCTGATCCGC
CAGCCGCACGGCATCGTGCTCGTTACCGGGCCGACAGGCTCGGGCAAGACCACTACGCTGTATGCGGCGCTATCTCGGCT
GGACGCGCGCTCCACCAACATCATGACGGTGGAGGATCCGATCGAATACGACCTCGACGGCATCGGCCAGACGCAGGTCA
ACGCGCGTATCGACATGACCTTCGCCAAGGCCCTGCGCGCGATCCTGCGCCAGGACCCGGACGTGGTGATGATCGGCGAA
ATCCGCGACCTGGAAACCGCGCAGATCGCGGTGCAGGCTTCGCTGACCGGCCACCTAGTGCTGGCCACGCTGCACACCAA
TGACTCGGCCTCGGCGATCACGCGGCTCGTGGACATGGGCATCGAGCCGTTCCTGCTGTCGTCGTCGCTGCTTGGCGTGC
TCGCGCAGCGGCTCGTACGCCGGTTGTGCCCGCATTGCAAGCGCGAGGAAGTCATCGAAGTCACCGCCGGCGAAGCCGAA
ACGCTGCATACGCAAGGCAAGCCGCTACAACACCTGTGGCATGCGGTGGGCTGCGACAAGTGCGGCAACTCCGGCTACCA
GGGCCGCATGGGCGTGTACGAGCTGCTGACCGTCGACGACGAGATCCGCACGATGATCCACCGCCAGCAGCCCGAGTCGG
AGATCAAGCAGGTCGCGCTTGCTCATGGCATGCAGACCATGCGCGGCGATGCGCAGCGCTGGATCGACACCGGCGCCACC
GCGCTCGAAGAAGTGCTGCGCGTCACGCGCGACTGA

Upstream 100 bases:

>100_bases
CGGCATCGCCCGACGCCGGCCGCCGTAACTGAGCCAGACCATGGCAAGCGAAGTCCAAGCCGCCCTTGACAGCCAGGATA
TTCAGGAGCCGCCGTCACCG

Downstream 100 bases:

>100_bases
CGGAACGCCGCCATGCCCGCCTTCCGCTACGAAGCCGCCGACGCCCTGGGCAAGACCGACCGCGGCGTCATCGAGGCCGA
CAGCCCGCGGCAGGCGCGCT

Product: type II secretion system protein E (GspE)

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MAARLVSYSFAREAPLLIAHQRADGLEVWVSRATSPAALAEVARVHGALRLRVMEPDALERAMSDAYNRQDGSAAQVVGE
VEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFESASVVRFRVDGTLRDVVRPKKALHGA
LISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDLAKLGMSPRTLAGFDDLIR
QPHGIVLVTGPTGSGKTTTLYAALSRLDARSTNIMTVEDPIEYDLDGIGQTQVNARIDMTFAKALRAILRQDPDVVMIGE
IRDLETAQIAVQASLTGHLVLATLHTNDSASAITRLVDMGIEPFLLSSSLLGVLAQRLVRRLCPHCKREEVIEVTAGEAE
TLHTQGKPLQHLWHAVGCDKCGNSGYQGRMGVYELLTVDDEIRTMIHRQQPESEIKQVALAHGMQTMRGDAQRWIDTGAT
ALEEVLRVTRD

Sequences:

>Translated_491_residues
MAARLVSYSFAREAPLLIAHQRADGLEVWVSRATSPAALAEVARVHGALRLRVMEPDALERAMSDAYNRQDGSAAQVVGE
VEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFESASVVRFRVDGTLRDVVRPKKALHGA
LISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDLAKLGMSPRTLAGFDDLIR
QPHGIVLVTGPTGSGKTTTLYAALSRLDARSTNIMTVEDPIEYDLDGIGQTQVNARIDMTFAKALRAILRQDPDVVMIGE
IRDLETAQIAVQASLTGHLVLATLHTNDSASAITRLVDMGIEPFLLSSSLLGVLAQRLVRRLCPHCKREEVIEVTAGEAE
TLHTQGKPLQHLWHAVGCDKCGNSGYQGRMGVYELLTVDDEIRTMIHRQQPESEIKQVALAHGMQTMRGDAQRWIDTGAT
ALEEVLRVTRD
>Mature_490_residues
AARLVSYSFAREAPLLIAHQRADGLEVWVSRATSPAALAEVARVHGALRLRVMEPDALERAMSDAYNRQDGSAAQVVGEV
EGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQAAREGASDIHIEPFESASVVRFRVDGTLRDVVRPKKALHGAL
ISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDLAKLGMSPRTLAGFDDLIRQ
PHGIVLVTGPTGSGKTTTLYAALSRLDARSTNIMTVEDPIEYDLDGIGQTQVNARIDMTFAKALRAILRQDPDVVMIGEI
RDLETAQIAVQASLTGHLVLATLHTNDSASAITRLVDMGIEPFLLSSSLLGVLAQRLVRRLCPHCKREEVIEVTAGEAET
LHTQGKPLQHLWHAVGCDKCGNSGYQGRMGVYELLTVDDEIRTMIHRQQPESEIKQVALAHGMQTMRGDAQRWIDTGATA
LEEVLRVTRD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=430, Percent_Identity=53.953488372093, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786296, Length=416, Percent_Identity=44.2307692307692, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=43.0769230769231, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53743; Mature: 53612

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARLVSYSFAREAPLLIAHQRADGLEVWVSRATSPAALAEVARVHGALRLRVMEPDALE
CCCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCHHHH
RAMSDAYNRQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQA
HHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHH
AREGASDIHIEPFESASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQ
HHCCCCCCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDLAKLGMSPRTLAGFDDLIR
CCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHCCCCCCHHCCHHHHHC
QPHGIVLVTGPTGSGKTTTLYAALSRLDARSTNIMTVEDPIEYDLDGIGQTQVNARIDMT
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEECCEEHHH
FAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAITRLVDMG
HHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEEEEEEECCCHHHHHHHHHHCC
IEPFLLSSSLLGVLAQRLVRRLCPHCKREEVIEVTAGEAETLHTQGKPLQHLWHAVGCDK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHCHHH
CGNSGYQGRMGVYELLTVDDEIRTMIHRQQPESEIKQVALAHGMQTMRGDAQRWIDTGAT
CCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEEVLRVTRD
HHHHHHHHHCC
>Mature Secondary Structure 
AARLVSYSFAREAPLLIAHQRADGLEVWVSRATSPAALAEVARVHGALRLRVMEPDALE
CCHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCHHHH
RAMSDAYNRQDGSAAQVVGEVEGEVDLSRLMQDIPAVEDLLESEDDAPIIRMINALLTQA
HHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHH
AREGASDIHIEPFESASVVRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQ
HHCCCCCCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLDKEAGRLDLAKLGMSPRTLAGFDDLIR
CCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHCCCCCCHHCCHHHHHC
QPHGIVLVTGPTGSGKTTTLYAALSRLDARSTNIMTVEDPIEYDLDGIGQTQVNARIDMT
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEECCEEHHH
FAKALRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLATLHTNDSASAITRLVDMG
HHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEEEEEEECCCHHHHHHHHHHCC
IEPFLLSSSLLGVLAQRLVRRLCPHCKREEVIEVTAGEAETLHTQGKPLQHLWHAVGCDK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHCHHH
CGNSGYQGRMGVYELLTVDDEIRTMIHRQQPESEIKQVALAHGMQTMRGDAQRWIDTGAT
CCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALEEVLRVTRD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]