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The map label for this gene is trpE [H]

Identifier: 73542707

GI number: 73542707

Start: 3325162

End: 3326679

Strand: Direct

Name: trpE [H]

Synonym: Reut_A3023

Alternate gene names: 73542707

Gene position: 3325162-3326679 (Clockwise)

Preceding gene: 73542706

Following gene: 73542708

Centisome position: 87.35

GC content: 64.95

Gene sequence:

>1518_bases
ATGACCGAACTGGAATTCAAATCGCTGGCCGACCAGGGCTACAACCGCATCCCGCTGATCGCCGAGGCCTTCGCCGATCT
GGAAACGCCGCTGTCGCTGTACCTGAAGCTGGCCCAGTCGCAGACGCGCGGCGTCAATACCTTCCTGCTGGAATCGGTGG
TGGGCGGCGAACGCTTCGGCCGCTACTCGTTTATCGGCCTGCATGCGCGCACCCTGCTGCGCTCCTACGGCAACCGTGCT
GAAGTTGTCACCGACGGCAAGGTGGTCGAGACCCACGAAGGCAACCCGCTTGATTTCATCGCCGAGTTCGAGAAGCGCTT
CAAAGTCGCGCTGCGCCCGGGCCTGCCGCGCTTCTGCGGCGGTCTGGCCGGCTATTTCGGCTATGACGCGGTGCGCTATA
TCGAGAAGAAGCTGGCCAACACGCAGAAGCCCGACGACCTCGGCCTGCCCGATATCCAGCTGCTGCTGTGCGAAGAGCTG
GCCGTGATCGACAACTTGTCCGGCAAGCTCTACCTCATCGTCTACGCCGACCCGACCACACCGGAAGCCTATTCCCGCGC
TCGCCAGCGCCTGCGTGAGCTGCGCATGAAGCTGCGCCAGCCCGTGGATGTGCCGGTAACGAGCCCGTCGGTGCAGACCG
ACGTCTACCGCGAGTTCGACAAGGCCGATTACCTGGCCGCCGTGCACAAGGCCAAGGAATACATCATGGCCGGCGACATG
ATGCAGGTACAAATCGGCCAGCGCCTGATGAAGCCGTACCGCGACGCGCCGCTGTCGCTGTACCGTGCGCTGCGCTCGCT
GAACCCGTCGCCGTACATGTACTTCTACAACTTCGGCGATTTCCAGATCGTCGGGGCCTCGCCCGAGATCCTGGTGCGCC
AGGAATCGCGCAAGGTCGGCACCAATGGCGATTCGCGCGACGAGCACATCGTCACGATCCGTCCGCTGGCCGGCACGCGT
CCGCGCGGCAATACGCCCGAGAAGGACGCGCAGCTCGCCACCGAACTGCTCAACGACCCGAAGGAGATCGCCGAGCATGT
GATGCTGATCGACCTGGCGCGCAACGATATCGGCCGCATCGCCGAGACCGGCTCGGTCAAGGTCACCGACAAGATGGTGA
TCGAAAAGTACTCGCACGTGCAGCACATCGTCAGCTCGGTCGAGGGCACCCTGCGCCCGGGCATGAGCAACCTCGACGTC
CTGCGCGCCACCTTCCCGGCCGGCACGCTGTCGGGCGCGCCCAAGGTGCACGCGATGGAGATCATCGACGAACTGGAGCC
GCGCAAGCGCGGCATCTACGGCGGCGCCGTGGGCTACCTGTCGTTCGGCGGCGAGATGGACCTGGCGATTGCGATCCGCA
CCGGCATCATCAAGGACGGCAATCTCTACGTGCAGGCCGCGGCCGGCATCGTTGCGGACTCGGACCCCGAAGCCGAATGG
AGGGAAACCGAAGCCAAGGCGCGCGCCGTGATCCGCGCGGCCGAGCAGGTCCAGGATGGCCTGGACTCCGACATCTGA

Upstream 100 bases:

>100_bases
GGTTCCGCAACCGGAGCGGCCTTGCGCCTGCTCCCGCCTAATTGCCGACACCACGGCGCCGCGCCAACAAGAACACGCCG
CGCCGCAAACGAGGATCGAC

Downstream 100 bases:

>100_bases
ACCAGGGAACAAGGGAGAAAAGGCCATGCTGCTGATGATCGACAACTACGATTCGTTCACCTACAACCTCGTCCAGTACT
TCGGCGAACTCGGCGAGGAC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTELEFKSLADQGYNRIPLIAEAFADLETPLSLYLKLAQSQTRGVNTFLLESVVGGERFGRYSFIGLHARTLLRSYGNRA
EVVTDGKVVETHEGNPLDFIAEFEKRFKVALRPGLPRFCGGLAGYFGYDAVRYIEKKLANTQKPDDLGLPDIQLLLCEEL
AVIDNLSGKLYLIVYADPTTPEAYSRARQRLRELRMKLRQPVDVPVTSPSVQTDVYREFDKADYLAAVHKAKEYIMAGDM
MQVQIGQRLMKPYRDAPLSLYRALRSLNPSPYMYFYNFGDFQIVGASPEILVRQESRKVGTNGDSRDEHIVTIRPLAGTR
PRGNTPEKDAQLATELLNDPKEIAEHVMLIDLARNDIGRIAETGSVKVTDKMVIEKYSHVQHIVSSVEGTLRPGMSNLDV
LRATFPAGTLSGAPKVHAMEIIDELEPRKRGIYGGAVGYLSFGGEMDLAIAIRTGIIKDGNLYVQAAAGIVADSDPEAEW
RETEAKARAVIRAAEQVQDGLDSDI

Sequences:

>Translated_505_residues
MTELEFKSLADQGYNRIPLIAEAFADLETPLSLYLKLAQSQTRGVNTFLLESVVGGERFGRYSFIGLHARTLLRSYGNRA
EVVTDGKVVETHEGNPLDFIAEFEKRFKVALRPGLPRFCGGLAGYFGYDAVRYIEKKLANTQKPDDLGLPDIQLLLCEEL
AVIDNLSGKLYLIVYADPTTPEAYSRARQRLRELRMKLRQPVDVPVTSPSVQTDVYREFDKADYLAAVHKAKEYIMAGDM
MQVQIGQRLMKPYRDAPLSLYRALRSLNPSPYMYFYNFGDFQIVGASPEILVRQESRKVGTNGDSRDEHIVTIRPLAGTR
PRGNTPEKDAQLATELLNDPKEIAEHVMLIDLARNDIGRIAETGSVKVTDKMVIEKYSHVQHIVSSVEGTLRPGMSNLDV
LRATFPAGTLSGAPKVHAMEIIDELEPRKRGIYGGAVGYLSFGGEMDLAIAIRTGIIKDGNLYVQAAAGIVADSDPEAEW
RETEAKARAVIRAAEQVQDGLDSDI
>Mature_504_residues
TELEFKSLADQGYNRIPLIAEAFADLETPLSLYLKLAQSQTRGVNTFLLESVVGGERFGRYSFIGLHARTLLRSYGNRAE
VVTDGKVVETHEGNPLDFIAEFEKRFKVALRPGLPRFCGGLAGYFGYDAVRYIEKKLANTQKPDDLGLPDIQLLLCEELA
VIDNLSGKLYLIVYADPTTPEAYSRARQRLRELRMKLRQPVDVPVTSPSVQTDVYREFDKADYLAAVHKAKEYIMAGDMM
QVQIGQRLMKPYRDAPLSLYRALRSLNPSPYMYFYNFGDFQIVGASPEILVRQESRKVGTNGDSRDEHIVTIRPLAGTRP
RGNTPEKDAQLATELLNDPKEIAEHVMLIDLARNDIGRIAETGSVKVTDKMVIEKYSHVQHIVSSVEGTLRPGMSNLDVL
RATFPAGTLSGAPKVHAMEIIDELEPRKRGIYGGAVGYLSFGGEMDLAIAIRTGIIKDGNLYVQAAAGIVADSDPEAEWR
ETEAKARAVIRAAEQVQDGLDSDI

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=384, Percent_Identity=39.0625, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788114, Length=452, Percent_Identity=32.5221238938053, Blast_Score=228, Evalue=1e-60,
Organism=Escherichia coli, GI1786809, Length=315, Percent_Identity=25.3968253968254, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082077, Length=274, Percent_Identity=26.2773722627737, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=505, Percent_Identity=39.6039603960396, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324361, Length=477, Percent_Identity=25.3668763102725, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 56117; Mature: 55986

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELEFKSLADQGYNRIPLIAEAFADLETPLSLYLKLAQSQTRGVNTFLLESVVGGERFG
CCCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHCC
RYSFIGLHARTLLRSYGNRAEVVTDGKVVETHEGNPLDFIAEFEKRFKVALRPGLPRFCG
CEEEEHHHHHHHHHHCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
GLAGYFGYDAVRYIEKKLANTQKPDDLGLPDIQLLLCEELAVIDNLSGKLYLIVYADPTT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
PEAYSRARQRLRELRMKLRQPVDVPVTSPSVQTDVYREFDKADYLAAVHKAKEYIMAGDM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MQVQIGQRLMKPYRDAPLSLYRALRSLNPSPYMYFYNFGDFQIVGASPEILVRQESRKVG
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEECCCCCC
TNGDSRDEHIVTIRPLAGTRPRGNTPEKDAQLATELLNDPKEIAEHVMLIDLARNDIGRI
CCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AETGSVKVTDKMVIEKYSHVQHIVSSVEGTLRPGMSNLDVLRATFPAGTLSGAPKVHAME
HCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCHHHHH
IIDELEPRKRGIYGGAVGYLSFGGEMDLAIAIRTGIIKDGNLYVQAAAGIVADSDPEAEW
HHHHCCHHHCCCCCCEEEHEECCCCEEEEEEEEECEEECCCEEEEEECCEEECCCCCCHH
RETEAKARAVIRAAEQVQDGLDSDI
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TELEFKSLADQGYNRIPLIAEAFADLETPLSLYLKLAQSQTRGVNTFLLESVVGGERFG
CCCCHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHCC
RYSFIGLHARTLLRSYGNRAEVVTDGKVVETHEGNPLDFIAEFEKRFKVALRPGLPRFCG
CEEEEHHHHHHHHHHCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHH
GLAGYFGYDAVRYIEKKLANTQKPDDLGLPDIQLLLCEELAVIDNLSGKLYLIVYADPTT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
PEAYSRARQRLRELRMKLRQPVDVPVTSPSVQTDVYREFDKADYLAAVHKAKEYIMAGDM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MQVQIGQRLMKPYRDAPLSLYRALRSLNPSPYMYFYNFGDFQIVGASPEILVRQESRKVG
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEECCCCCC
TNGDSRDEHIVTIRPLAGTRPRGNTPEKDAQLATELLNDPKEIAEHVMLIDLARNDIGRI
CCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AETGSVKVTDKMVIEKYSHVQHIVSSVEGTLRPGMSNLDVLRATFPAGTLSGAPKVHAME
HCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCHHHHH
IIDELEPRKRGIYGGAVGYLSFGGEMDLAIAIRTGIIKDGNLYVQAAAGIVADSDPEAEW
HHHHCCHHHCCCCCCEEEHEECCCCEEEEEEEEECEEECCCEEEEEECCEEECCCCCCHH
RETEAKARAVIRAAEQVQDGLDSDI
HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]