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The map label for this gene is gph [C]

Identifier: 73542706

GI number: 73542706

Start: 3324004

End: 3324729

Strand: Direct

Name: gph [C]

Synonym: Reut_A3022

Alternate gene names: 73542706

Gene position: 3324004-3324729 (Clockwise)

Preceding gene: 73542705

Following gene: 73542707

Centisome position: 87.32

GC content: 65.84

Gene sequence:

>726_bases
ATGACGCTGCGCCGCACCGACTGGCGCGGCATCGAAGGCGTGATCGTCGATCTGGACGGCACCATGGTCGACACCGCCGG
GGATTTCCACGCGTCGATCAACGCGATGCTGCTGGCGCTCGCGCACCTGCACCCGAACCTGGGGCCGGTCGCGCCGATGT
CCGAGCAGGAGATCGTGAGCTTCGTCGGCAAGGGTTCGGAGAACCTGATCCGGCGCGTGCTGGACGCCCGGTTCTCGCCA
CTGCACGCCAACGGCCTGTTCGCGCAGGCCTATGCGCTGTACGACCGCGAGTACATCCGCATCAATGGCCAGTTCTCGCA
GATCTACCCCGGCGTGCGCGAAGGCCTGGCGGCGCTGAAGGCGGCCGGCCTGCGCATGGCCTGCGTGACCAACAAGCCGT
ACAACTTCACCGAGCCGCTGCTGGCCAAGACCGGGCTGGCGCAGTATTTCGAGCTGGTCTACGGTGGCGATGCGTTTCCG
CTGCGCAAGCCGGACCCGTATCCGCTGCTCAAGGTGGCCGAGGTTTTCCACCTCGATCCGTCGGCCATGCTGGCCATCGG
CGACTCGGAAAACGACGCGCAGGCGGCCCGCGCGGCCGGCATGGGTGTGCTGCTGATGCCCTACGGCTACAACCATGGCA
ACCCTGTACAAGCCGTCGACGCCGATGGTATAGTCGATTCCATTGCCCGCGTGGCAGAGCTTCTTTCTGCACACGGAACC
TGCTGA

Upstream 100 bases:

>100_bases
GCTCGGCCGTGTTCGGCAAGCCCGATGCGGACGGCGGCTACCGCGGCATCATTTCCGCACTGCGCGGCGAGCTGGCCAAG
GTCGGCCAGTGAGCGCCGGC

Downstream 100 bases:

>100_bases
GAAAGCGGTAATTCGCTGGCAATCAGACTTTCATAGCCCCTGAACACCTCAATGTTCTTCAACCGCAAACGCATCTCTTC
TGGCAGTGATCGGCAGGCTT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTLRRTDWRGIEGVIVDLDGTMVDTAGDFHASINAMLLALAHLHPNLGPVAPMSEQEIVSFVGKGSENLIRRVLDARFSP
LHANGLFAQAYALYDREYIRINGQFSQIYPGVREGLAALKAAGLRMACVTNKPYNFTEPLLAKTGLAQYFELVYGGDAFP
LRKPDPYPLLKVAEVFHLDPSAMLAIGDSENDAQAARAAGMGVLLMPYGYNHGNPVQAVDADGIVDSIARVAELLSAHGT
C

Sequences:

>Translated_241_residues
MTLRRTDWRGIEGVIVDLDGTMVDTAGDFHASINAMLLALAHLHPNLGPVAPMSEQEIVSFVGKGSENLIRRVLDARFSP
LHANGLFAQAYALYDREYIRINGQFSQIYPGVREGLAALKAAGLRMACVTNKPYNFTEPLLAKTGLAQYFELVYGGDAFP
LRKPDPYPLLKVAEVFHLDPSAMLAIGDSENDAQAARAAGMGVLLMPYGYNHGNPVQAVDADGIVDSIARVAELLSAHGT
C
>Mature_240_residues
TLRRTDWRGIEGVIVDLDGTMVDTAGDFHASINAMLLALAHLHPNLGPVAPMSEQEIVSFVGKGSENLIRRVLDARFSPL
HANGLFAQAYALYDREYIRINGQFSQIYPGVREGLAALKAAGLRMACVTNKPYNFTEPLLAKTGLAQYFELVYGGDAFPL
RKPDPYPLLKVAEVFHLDPSAMLAIGDSENDAQAARAAGMGVLLMPYGYNHGNPVQAVDADGIVDSIARVAELLSAHGTC

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=237, Percent_Identity=36.2869198312236, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 26028; Mature: 25897

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRRTDWRGIEGVIVDLDGTMVDTAGDFHASINAMLLALAHLHPNLGPVAPMSEQEIVS
CCCCCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
FVGKGSENLIRRVLDARFSPLHANGLFAQAYALYDREYIRINGQFSQIYPGVREGLAALK
HHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCHHHHCCCHHHHHHHHH
AAGLRMACVTNKPYNFTEPLLAKTGLAQYFELVYGGDAFPLRKPDPYPLLKVAEVFHLDP
HCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCC
SAMLAIGDSENDAQAARAAGMGVLLMPYGYNHGNPVQAVDADGIVDSIARVAELLSAHGT
CCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCC
C
C
>Mature Secondary Structure 
TLRRTDWRGIEGVIVDLDGTMVDTAGDFHASINAMLLALAHLHPNLGPVAPMSEQEIVS
CCCCCCCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
FVGKGSENLIRRVLDARFSPLHANGLFAQAYALYDREYIRINGQFSQIYPGVREGLAALK
HHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCHHHHCCCHHHHHHHHH
AAGLRMACVTNKPYNFTEPLLAKTGLAQYFELVYGGDAFPLRKPDPYPLLKVAEVFHLDP
HCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCC
SAMLAIGDSENDAQAARAAGMGVLLMPYGYNHGNPVQAVDADGIVDSIARVAELLSAHGT
CCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA