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The map label for this gene is purN [H]

Identifier: 73542426

GI number: 73542426

Start: 3008846

End: 3009511

Strand: Reverse

Name: purN [H]

Synonym: Reut_A2741

Alternate gene names: 73542426

Gene position: 3009511-3008846 (Counterclockwise)

Preceding gene: 73542428

Following gene: 73542425

Centisome position: 79.06

GC content: 62.76

Gene sequence:

>666_bases
ATGAAAAAAATCGTAATCCTGATTTCAGGGCGTGGCTCCAATATGGAAGCCATCGTCCGGGCATGCGCGGCCGGGGGTTG
GCCGGCGCGCATTGCCGCAGTCATTTCCAACCGGCCGGATGCCGCCGGACTGCAGTTTGCCAAGGACCACGGCATTGCCG
CCGGCGTGGTCGATCATCGCCAGCATCCCGACCGCGCCAGCTTCGATGCGGCGCTGGCCGAGGCCATTGATGCCTACGAA
CCCGATCTGGTCGTGCTGGCCGGCTTTATGCGCATCCTGACGACCGGCTTTGTCGATCGTTATGCCGGCCGACTGCTCAA
TATCCATCCGTCGCTGCTGCCGTGCTTCCCGGGTCTGAACACCCATAAGCAGGCGCTCGACGCAGGCGTGAAGCTGCACG
GTGCGACCGTGCATTTCGTGACCCCGGAACTCGATCACGGCCCGATCGTGATCCAGGCGGGCCTCGATGTATTGCCAAAC
GATACTCCAGAGAGCCTCGCCGCACGCCTGCTCGACTGCGAGCATGTCATTTATCCCCGCGCCGTCCAGTGGTTCGTCGA
AGGACGCCTGCAGGTGCAAAACGGGGTCGTACACGTCAATCCGGCCGAGCCGCAACTGCTTATGGCCATCTCCGCCGGTG
TACCGGCGGCAGGAGCAAAGCCATGA

Upstream 100 bases:

>100_bases
CGCATCCCGGTTGCGCCCCGAATCTCGGATTGTCGTGAATTAAGTGCCTAATGTATTCGCTTTGGCAGAGTTTAGTGCTT
GCACTTGGTAGAATTGCGCG

Downstream 100 bases:

>100_bases
GTCGTCATCAGGCCGGGGCGCGCCCGGCGCCCAAGGATTCCCGCCAACCTGCTAAAGCCCGCAAGTCGAGCCCGATCCGC
AGGCCGGCAAACGCGCGGTC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKKIVILISGRGSNMEAIVRACAAGGWPARIAAVISNRPDAAGLQFAKDHGIAAGVVDHRQHPDRASFDAALAEAIDAYE
PDLVVLAGFMRILTTGFVDRYAGRLLNIHPSLLPCFPGLNTHKQALDAGVKLHGATVHFVTPELDHGPIVIQAGLDVLPN
DTPESLAARLLDCEHVIYPRAVQWFVEGRLQVQNGVVHVNPAEPQLLMAISAGVPAAGAKP

Sequences:

>Translated_221_residues
MKKIVILISGRGSNMEAIVRACAAGGWPARIAAVISNRPDAAGLQFAKDHGIAAGVVDHRQHPDRASFDAALAEAIDAYE
PDLVVLAGFMRILTTGFVDRYAGRLLNIHPSLLPCFPGLNTHKQALDAGVKLHGATVHFVTPELDHGPIVIQAGLDVLPN
DTPESLAARLLDCEHVIYPRAVQWFVEGRLQVQNGVVHVNPAEPQLLMAISAGVPAAGAKP
>Mature_221_residues
MKKIVILISGRGSNMEAIVRACAAGGWPARIAAVISNRPDAAGLQFAKDHGIAAGVVDHRQHPDRASFDAALAEAIDAYE
PDLVVLAGFMRILTTGFVDRYAGRLLNIHPSLLPCFPGLNTHKQALDAGVKLHGATVHFVTPELDHGPIVIQAGLDVLPN
DTPESLAARLLDCEHVIYPRAVQWFVEGRLQVQNGVVHVNPAEPQLLMAISAGVPAAGAKP

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=196, Percent_Identity=41.8367346938776, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869995, Length=196, Percent_Identity=41.8367346938776, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI209869993, Length=196, Percent_Identity=41.8367346938776, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=49.740932642487, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787483, Length=191, Percent_Identity=36.6492146596859, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=37.2222222222222, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=200, Percent_Identity=32, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=45.5026455026455, Blast_Score=165, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23467; Mature: 23467

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVILISGRGSNMEAIVRACAAGGWPARIAAVISNRPDAAGLQFAKDHGIAAGVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEHHCCCCEECHHHCC
QHPDRASFDAALAEAIDAYEPDLVVLAGFMRILTTGFVDRYAGRLLNIHPSLLPCFPGLN
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCC
THKQALDAGVKLHGATVHFVTPELDHGPIVIQAGLDVLPNDTPESLAARLLDCEHVIYPR
HHHHHHHCCCEEECCEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AVQWFVEGRLQVQNGVVHVNPAEPQLLMAISAGVPAAGAKP
HHHHHHHCEEEEECCEEEECCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKIVILISGRGSNMEAIVRACAAGGWPARIAAVISNRPDAAGLQFAKDHGIAAGVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEHHCCCCEECHHHCC
QHPDRASFDAALAEAIDAYEPDLVVLAGFMRILTTGFVDRYAGRLLNIHPSLLPCFPGLN
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHCCCCCC
THKQALDAGVKLHGATVHFVTPELDHGPIVIQAGLDVLPNDTPESLAARLLDCEHVIYPR
HHHHHHHCCCEEECCEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AVQWFVEGRLQVQNGVVHVNPAEPQLLMAISAGVPAAGAKP
HHHHHHHCEEEEECCEEEECCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]