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The map label for this gene is guaD [H]

Identifier: 73542115

GI number: 73542115

Start: 2662487

End: 2663809

Strand: Direct

Name: guaD [H]

Synonym: Reut_A2429

Alternate gene names: 73542115

Gene position: 2662487-2663809 (Clockwise)

Preceding gene: 73542114

Following gene: 73542116

Centisome position: 69.95

GC content: 66.97

Gene sequence:

>1323_bases
ATGACGACCACTCCTCCGACTCCGTCCATTACTCGCGCGATCCGCGGCCGCGTGCTGCATTTCCTGCACGACCCGCAGTT
CCATGAGGACGCCTATCAGTATTGGGAGGATGGTCTGCTGCTCGTCACCGCTGGCCGCATCGTCGCGGCCGGCGACTACA
CGTCGCTCGCGTCGCGCATTCCGGCCGATGCCGAGGTGATCGACCACCGCGGCAAGCTGATCGTGCCGGGCTTTATCGAC
ACGCACGTGCACTACCCGCAGACCGACATCATCGCGTCGCCGTCGCCGGGCCTGCTGCACTGGCTGGACACCTACACCTT
CCCCGAGGAACGTCGCTTCGCCGAGCCTGAATACGCGCGCGCGGTCGCCGGCTTCTTCACCGACGAACTGCTGCGCAACG
GCACCACCAGCGCGGTGGTCTGGAGCACGGTGCACAAGGCTTCAGCCGACGCACTGTTTGCCGAGAGCGAACAGCGCAAC
CTGCGCATGATCACCGGCAAGGTGATGATGGACCGCAACTGTCCCGAGTTCCTGCGCGATACCGCTGAAACCGGCGCGCG
CGATTCCGCCGACCTGCTGTCGCGCTGGCATAACAAGGGCCGCCTCGCCTACGCCATCACGCCGCGCTTCGCGCCGACGT
CGACCGAAGCGCAGCTCGCCGCCTGCGGCGAACTGGCCAAGGCCTATCCGGACGCATTCATCCAGACCCACGTCGCAGAG
AACCGCGACGAAGTGAAATGGGTGGCCGAACTGTTCCCGGACGCGCGGAGCTACCTGGACGTCTACGACCGCTACGGCCT
GCTGCGCCCCGGCGCGTTCTACGGCCATGCGATCTACCTGGACCAGGACGACCGCCGCCGCCTGGCCGACAGCGGCGCCG
CCGTCGCGCACTGCCCGACGTCGAACCTGTTCCTCGGCAGCGGCTTCTACGACTTCCACCAGTCGGACGCCAACCGCCTG
AACGTGACGCTGGCGACCGACGTCGGCGGCGGCACCTCGTTCTCGATGTTCCGCACCATGAACGCCGCGCACAAGGTCGC
GCGCATGGGCGGCTACCACCTGACCGCGCTGCGCATGTTTTACCTGGCCACGCGCGCGGCCGCCGAAGCGCTGGGCTGGT
CCAACCGCATCGGCAGCTTCAGCGAGGGCTGCGAGGCCGACTTCATCGTGCTGGATCCGCAGGCCACGCCGCTGATCGCG
CGTCGCAGCGGCCGCTCGGAGACGCTCGAAGAGCAATTGTTCGCATTCGCCATGCTCGGCGATGACCGGGTCATCGACAG
TGTCTATGTGATGGGCGAGGCGGTACAGGTACCGGTCGTGTGA

Upstream 100 bases:

>100_bases
ACGCCGACGTCCCCGCCTGCCACGGCCCCCGCGGCCTGAGCGGCACATACCAATGGCCTGGCCCTGCGCGTGCGCGCCGG
CCTGGCCCGAGAGACAGACC

Downstream 100 bases:

>100_bases
CGCCCTCCATACCTGTGGCCGCCACTCGTGCGGCCGCGATGGGTTAATACCCGGGAGCGCTCCGGCGCGGCTTGACGGTG
ATCAAGACTCGTAACGGCAA

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTTTPPTPSITRAIRGRVLHFLHDPQFHEDAYQYWEDGLLLVTAGRIVAAGDYTSLASRIPADAEVIDHRGKLIVPGFID
THVHYPQTDIIASPSPGLLHWLDTYTFPEERRFAEPEYARAVAGFFTDELLRNGTTSAVVWSTVHKASADALFAESEQRN
LRMITGKVMMDRNCPEFLRDTAETGARDSADLLSRWHNKGRLAYAITPRFAPTSTEAQLAACGELAKAYPDAFIQTHVAE
NRDEVKWVAELFPDARSYLDVYDRYGLLRPGAFYGHAIYLDQDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQSDANRL
NVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWSNRIGSFSEGCEADFIVLDPQATPLIA
RRSGRSETLEEQLFAFAMLGDDRVIDSVYVMGEAVQVPVV

Sequences:

>Translated_440_residues
MTTTPPTPSITRAIRGRVLHFLHDPQFHEDAYQYWEDGLLLVTAGRIVAAGDYTSLASRIPADAEVIDHRGKLIVPGFID
THVHYPQTDIIASPSPGLLHWLDTYTFPEERRFAEPEYARAVAGFFTDELLRNGTTSAVVWSTVHKASADALFAESEQRN
LRMITGKVMMDRNCPEFLRDTAETGARDSADLLSRWHNKGRLAYAITPRFAPTSTEAQLAACGELAKAYPDAFIQTHVAE
NRDEVKWVAELFPDARSYLDVYDRYGLLRPGAFYGHAIYLDQDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQSDANRL
NVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWSNRIGSFSEGCEADFIVLDPQATPLIA
RRSGRSETLEEQLFAFAMLGDDRVIDSVYVMGEAVQVPVV
>Mature_439_residues
TTTPPTPSITRAIRGRVLHFLHDPQFHEDAYQYWEDGLLLVTAGRIVAAGDYTSLASRIPADAEVIDHRGKLIVPGFIDT
HVHYPQTDIIASPSPGLLHWLDTYTFPEERRFAEPEYARAVAGFFTDELLRNGTTSAVVWSTVHKASADALFAESEQRNL
RMITGKVMMDRNCPEFLRDTAETGARDSADLLSRWHNKGRLAYAITPRFAPTSTEAQLAACGELAKAYPDAFIQTHVAEN
RDEVKWVAELFPDARSYLDVYDRYGLLRPGAFYGHAIYLDQDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQSDANRLN
VTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWSNRIGSFSEGCEADFIVLDPQATPLIAR
RSGRSETLEEQLFAFAMLGDDRVIDSVYVMGEAVQVPVV

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=392, Percent_Identity=34.4387755102041, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1789249, Length=427, Percent_Identity=46.3700234192037, Blast_Score=424, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319963, Length=358, Percent_Identity=31.2849162011173, Blast_Score=173, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24643849, Length=447, Percent_Identity=32.8859060402685, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 48828; Mature: 48697

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPPTPSITRAIRGRVLHFLHDPQFHEDAYQYWEDGLLLVTAGRIVAAGDYTSLASRI
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCEEEECCHHHHHHHC
PADAEVIDHRGKLIVPGFIDTHVHYPQTDIIASPSPGLLHWLDTYTFPEERRFAEPEYAR
CCCHHHHHCCCCEEECCHHHCCCCCCCCCEECCCCCCHHHHHHHCCCCHHHCCCCHHHHH
AVAGFFTDELLRNGTTSAVVWSTVHKASADALFAESEQRNLRMITGKVMMDRNCPEFLRD
HHHHHHHHHHHHCCCCCCEEHHHHHHHCCHHHHHCCCCCCEEEEEEEEEECCCCHHHHHH
TAETGARDSADLLSRWHNKGRLAYAITPRFAPTSTEAQLAACGELAKAYPDAFIQTHVAE
HHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHC
NRDEVKWVAELFPDARSYLDVYDRYGLLRPGAFYGHAIYLDQDDRRRLADSGAAVAHCPT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCEEEECCC
SNLFLGSGFYDFHQSDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMF
CCEEEECCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
YLATRAAAEALGWSNRIGSFSEGCEADFIVLDPQATPLIARRSGRSETLEEQLFAFAMLG
HHHHHHHHHHHCCHHCCCCCCCCCCCCEEEECCCCCCCCEECCCCCHHHHHHHHHHHHCC
DDRVIDSVYVMGEAVQVPVV
CCHHHHHHHEECCEEEECCC
>Mature Secondary Structure 
TTTPPTPSITRAIRGRVLHFLHDPQFHEDAYQYWEDGLLLVTAGRIVAAGDYTSLASRI
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCEEEECCHHHHHHHC
PADAEVIDHRGKLIVPGFIDTHVHYPQTDIIASPSPGLLHWLDTYTFPEERRFAEPEYAR
CCCHHHHHCCCCEEECCHHHCCCCCCCCCEECCCCCCHHHHHHHCCCCHHHCCCCHHHHH
AVAGFFTDELLRNGTTSAVVWSTVHKASADALFAESEQRNLRMITGKVMMDRNCPEFLRD
HHHHHHHHHHHHCCCCCCEEHHHHHHHCCHHHHHCCCCCCEEEEEEEEEECCCCHHHHHH
TAETGARDSADLLSRWHNKGRLAYAITPRFAPTSTEAQLAACGELAKAYPDAFIQTHVAE
HHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHC
NRDEVKWVAELFPDARSYLDVYDRYGLLRPGAFYGHAIYLDQDDRRRLADSGAAVAHCPT
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHCCCCEEEECCC
SNLFLGSGFYDFHQSDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMF
CCEEEECCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
YLATRAAAEALGWSNRIGSFSEGCEADFIVLDPQATPLIARRSGRSETLEEQLFAFAMLG
HHHHHHHHHHHCCHHCCCCCCCCCCCCEEEECCCCCCCCEECCCCCHHHHHHHHHHHHCC
DDRVIDSVYVMGEAVQVPVV
CCHHHHHHHEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]