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The map label for this gene is purF [H]

Identifier: 73541985

GI number: 73541985

Start: 2535534

End: 2537060

Strand: Reverse

Name: purF [H]

Synonym: Reut_A2299

Alternate gene names: 73541985

Gene position: 2537060-2535534 (Counterclockwise)

Preceding gene: 73541986

Following gene: 73541984

Centisome position: 66.65

GC content: 64.44

Gene sequence:

>1527_bases
ATGTGCGGTATCGTCGGCGCGGTCTCTACCTCGCCCGTCAACCAATTGATTTACGACAGCCTGCTGTTGTTGCAACACCG
CGGACAGGATGCTGCCGGCATCGCCACCGCCAACGGCAGTACTTTCCACATGCACAAGGCCAACGGCCTCGTGCGCGACG
TGTTCCGGACCCGCAATATGCGCGGCCTGCCGGGCACCGCCGGCATCGGTCAGGTGCGTTACCCGACCGCGGGCTCCGCC
TCCAGCGAAGAAGAGGCACAGCCGTTCTACGTCAATGCGCCGTACGGCATCATCCTGGCCCACAACGGCAACCTGACCAA
CTGGCAGCAACTGCGCGAGGAAATGTTCCGCCGCGACCGCCGCCACATCAATACGCACTCGGATACCGAGGTGCTGCTCA
ATGTGCTCGCTGACGAACTGCAGCGCGCGAGCCAGGGCATGGCGCTGGATCCGGAAACGATCTTCAAGGCCGTTTCCGGC
ATGCACCGCCGCGTGAAGGGCTCGTACGCCATTGCCGCGCAGATCGCCGGTTACGGCATGCTTGCCGTGCGCGATCCGTT
CGGCATCCGCCCGCTGAGTCTTGGCAGCGTGGACACGCCCACCGGCAAGGAATGGATGGTGGCGTCGGAGTCGGTGGCGC
TGGAAGGCATCGGCTATCGCATGGAGCGCGACGTGGCGCCCGGCGAGGCCATCTTCATCGACCTCGACGGCAAGCTGTAC
AGCAAGCAGTGCGCGGACAACCCCGTGCTGACGCCGTGCATCTTCGAGTACGTCTACCTGGCCCGTCCGGATTCGTGCAT
CGATGGCGTGCCCGTCTATGACGCCCGCCTGCGCATGGGCGACTACCTGGCCGAAAAGATCCGCCAGGAAGTGTCGGCCG
GCGACATCGACGTGGTCATGCCGATTCCCGATTCCAGCCGCCCGGCCGCCATGCAGGTAGCCAACCGGCTGGGCGTGGGC
TATCGCGAAGGTTTCTTCAAGAACCGCTACGTGGGCCGCACCTTCATCATGCCGGGCCAGGCGGTGCGCAAGAAGTCGGT
GCGCCAGAAGCTCAACGCGATGGCCGTCGAGTTCAAGGGCAAGAACGTGCTGATCGTCGATGACTCGATCGTGCGCGGCA
CCACGTCGTTCGAGATCGTGCAGATGGCGCGCGACGCCGGCGCCAACAAGGTCATCTTCGCCTCGGCCGCGCCGCCGGTG
AAGTACCCGAACGTGTACGGCATTGACATGCCGACGCGCAGCGAGTTGGTGGCCCATGGCCGCACGCACGAAGAGATCGC
CAAGATCATCGGCGCCGACAAGCTGGTGTACCAGGACGTGGAAGCGATGAAGCAGGCTGTACGCGACATCAACCCCGCGT
TGAACGACTTCGACGCGTCGTGCTTCGATGGCCGCTACGTTACCGGCGACATCGACGAGGCCTACCTGGATCGCCTGGAA
ACGGCGCGCAGCTTGGCTGATCGCGAAGGCACCGGAGACATGGAGCGCTCGCAACTGCACCTGCAGCGTTCGGGCGGCAA
CGAGTAA

Upstream 100 bases:

>100_bases
CACTGAGGCCCGGCACCGATGCCTGGCCGGTACGAAACGCAGGCCCTGTCCTCAGGACCGGGCTGCGCCCCTGATTCAAA
GCCTTTCCTGGGAGCATGGC

Downstream 100 bases:

>100_bases
GCCGCGCCGCGTCCGTGCGCGTTGACGAATGCGAGGCCCGGCCGTAACATGGCCGGGCGTCGATTTGAGAGTCAGCTTGC
GGACGCGGGTCCTGCCCGAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGLVRDVFRTRNMRGLPGTAGIGQVRYPTAGSA
SSEEEAQPFYVNAPYGIILAHNGNLTNWQQLREEMFRRDRRHINTHSDTEVLLNVLADELQRASQGMALDPETIFKAVSG
MHRRVKGSYAIAAQIAGYGMLAVRDPFGIRPLSLGSVDTPTGKEWMVASESVALEGIGYRMERDVAPGEAIFIDLDGKLY
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRQEVSAGDIDVVMPIPDSSRPAAMQVANRLGVG
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAVEFKGKNVLIVDDSIVRGTTSFEIVQMARDAGANKVIFASAAPPV
KYPNVYGIDMPTRSELVAHGRTHEEIAKIIGADKLVYQDVEAMKQAVRDINPALNDFDASCFDGRYVTGDIDEAYLDRLE
TARSLADREGTGDMERSQLHLQRSGGNE

Sequences:

>Translated_508_residues
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGLVRDVFRTRNMRGLPGTAGIGQVRYPTAGSA
SSEEEAQPFYVNAPYGIILAHNGNLTNWQQLREEMFRRDRRHINTHSDTEVLLNVLADELQRASQGMALDPETIFKAVSG
MHRRVKGSYAIAAQIAGYGMLAVRDPFGIRPLSLGSVDTPTGKEWMVASESVALEGIGYRMERDVAPGEAIFIDLDGKLY
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRQEVSAGDIDVVMPIPDSSRPAAMQVANRLGVG
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAVEFKGKNVLIVDDSIVRGTTSFEIVQMARDAGANKVIFASAAPPV
KYPNVYGIDMPTRSELVAHGRTHEEIAKIIGADKLVYQDVEAMKQAVRDINPALNDFDASCFDGRYVTGDIDEAYLDRLE
TARSLADREGTGDMERSQLHLQRSGGNE
>Mature_508_residues
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGLVRDVFRTRNMRGLPGTAGIGQVRYPTAGSA
SSEEEAQPFYVNAPYGIILAHNGNLTNWQQLREEMFRRDRRHINTHSDTEVLLNVLADELQRASQGMALDPETIFKAVSG
MHRRVKGSYAIAAQIAGYGMLAVRDPFGIRPLSLGSVDTPTGKEWMVASESVALEGIGYRMERDVAPGEAIFIDLDGKLY
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRQEVSAGDIDVVMPIPDSSRPAAMQVANRLGVG
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAVEFKGKNVLIVDDSIVRGTTSFEIVQMARDAGANKVIFASAAPPV
KYPNVYGIDMPTRSELVAHGRTHEEIAKIIGADKLVYQDVEAMKQAVRDINPALNDFDASCFDGRYVTGDIDEAYLDRLE
TARSLADREGTGDMERSQLHLQRSGGNE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=488, Percent_Identity=37.0901639344262, Blast_Score=273, Evalue=4e-73,
Organism=Escherichia coli, GI1788651, Length=486, Percent_Identity=59.2592592592593, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790167, Length=171, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=489, Percent_Identity=34.1513292433538, Blast_Score=239, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=491, Percent_Identity=49.2871690427699, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24659598, Length=506, Percent_Identity=35.5731225296443, Blast_Score=279, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24659604, Length=486, Percent_Identity=36.2139917695473, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI28573187, Length=502, Percent_Identity=35.4581673306773, Blast_Score=269, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55816; Mature: 55816

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGLVRDVFRTRNM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RGLPGTAGIGQVRYPTAGSASSEEEAQPFYVNAPYGIILAHNGNLTNWQQLREEMFRRDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHINTHSDTEVLLNVLADELQRASQGMALDPETIFKAVSGMHRRVKGSYAIAAQIAGYGM
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHCCCCE
LAVRDPFGIRPLSLGSVDTPTGKEWMVASESVALEGIGYRMERDVAPGEAIFIDLDGKLY
EEEECCCCCCEEECCCCCCCCCCCEEEECCCEEEECCCCEEECCCCCCCEEEEEECCCCH
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRQEVSAGDIDVVM
HHHHCCCCCCHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PIPDSSRPAAMQVANRLGVGYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAVEFKG
ECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KNVLIVDDSIVRGTTSFEIVQMARDAGANKVIFASAAPPVKYPNVYGIDMPTRSELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
RTHEEIAKIIGADKLVYQDVEAMKQAVRDINPALNDFDASCFDGRYVTGDIDEAYLDRLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEEECCCCHHHHHHHH
TARSLADREGTGDMERSQLHLQRSGGNE
HHHHHHHCCCCCCCCHHHHHEECCCCCC
>Mature Secondary Structure
MCGIVGAVSTSPVNQLIYDSLLLLQHRGQDAAGIATANGSTFHMHKANGLVRDVFRTRNM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHCCC
RGLPGTAGIGQVRYPTAGSASSEEEAQPFYVNAPYGIILAHNGNLTNWQQLREEMFRRDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHINTHSDTEVLLNVLADELQRASQGMALDPETIFKAVSGMHRRVKGSYAIAAQIAGYGM
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHCCCCE
LAVRDPFGIRPLSLGSVDTPTGKEWMVASESVALEGIGYRMERDVAPGEAIFIDLDGKLY
EEEECCCCCCEEECCCCCCCCCCCEEEECCCEEEECCCCEEECCCCCCCEEEEEECCCCH
SKQCADNPVLTPCIFEYVYLARPDSCIDGVPVYDARLRMGDYLAEKIRQEVSAGDIDVVM
HHHHCCCCCCHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
PIPDSSRPAAMQVANRLGVGYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAVEFKG
ECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KNVLIVDDSIVRGTTSFEIVQMARDAGANKVIFASAAPPVKYPNVYGIDMPTRSELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
RTHEEIAKIIGADKLVYQDVEAMKQAVRDINPALNDFDASCFDGRYVTGDIDEAYLDRLE
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEEECCCCHHHHHHHH
TARSLADREGTGDMERSQLHLQRSGGNE
HHHHHHHCCCCCCCCHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]