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The map label for this gene is metZ [H]

Identifier: 73541984

GI number: 73541984

Start: 2534042

End: 2535247

Strand: Reverse

Name: metZ [H]

Synonym: Reut_A2298

Alternate gene names: 73541984

Gene position: 2535247-2534042 (Counterclockwise)

Preceding gene: 73541985

Following gene: 73541979

Centisome position: 66.6

GC content: 64.18

Gene sequence:

>1206_bases
ATGAACCAACCGCTCAATCCTGAATCGCTTGGCATCGATACCCTTGGCGTGCGCGCCGGCACGCTGCGCAGCGAATACAT
GGAGCATTCCGAGGCGATGTACCTGACCTCGAGCTTCTGCTTCCACAGCGCCGCCGAAGCCGCGGAACGCTTCGCCAACT
CGGAAGAGGGCTTCACCTACTCGCGCTTCACCAACCCGACCGTCTCGATGTTCCAGTCGCGCCTGGCGGCGCTGGAAGGC
GCGGAGTCGTGCATGGCGACCGCGTCGGGCATGAGCGCGATCCTGTCGGTGGTGATGTCGCTGATGCAGGCGGGCGACCA
CCTGGTGAGCTCGCGCGCGATCTTCGGGTCGACCATGACGCTGTTCGGGAACATTTTCTCGAAGTTCGGCGTCGAGACGA
CCTTCGTCGACCCGGGCGATCTGAACGCTTGGCGCGCGGCAGTGCGACCGACCACGAAGCTGTTCTTCCTGGAGACCCCG
TCGAATCCGTTGACCGAGGTAGCGGACATCGCGGCCGTGGCCGACATCGCGCACAACGCCGGCGCCCAATTGATCGTCGA
CAACTGCTTCTGTTCGCCAGCGCTGCAGCAGCCGATCAAGTTTGGCGCCGACATCGTGGTGCATTCGGCCACTAAGCATA
TCGACGGGCAGGGCCGTGTGCTCGGCGGCGCGGTAGTCGGTTCGCACGATTTCATCATGGGCAAGGTTTTCCCGTTCGTG
CGTACCGCGGGTCCGACGCTGTCGGCCTTCAACGCGTGGGTGCTCCTCAAGGGCATGGAGACGCTGGCCATCCGCATGGA
GCGGCATTCTGCCAATGCGCTGGCGCTCGCCGAGTTCCTGGAATCGCATCCGGCAGTGGCACGCGTGTTCCATCCCGCGC
TGAAGTCGCATCCGCAGCATGAGATCGCCATGCGTCAACAGAGCGGTGGTGGCGCGATCGTGTCGTTCGAGCTCAAGGGT
GCGACGCCCGAGCAGCAGCGTGCGAATGCCTGGCGCGTGATCGACAACACGCGCTTGTGCTCGATCACCGGGAATCTCGG
TGATACGCGTACGACGATTACCCATCCGTACACGACCACGCACGCGCGTGTGAGTCCGGAGGCCAAGGCAGCTGCGGGTG
TCGGTGAAGGCCTGATCCGCCTGGCGGTGGGACTTGAGACGGTGGCCGATCTGAAGGCCGATCTGCTGCGCGGGCTCGGC
GAGTAA

Upstream 100 bases:

>100_bases
GATCCGACTCTCCGATCGCACGGGCTTTTTTCATGGCCGGCCGTTTGCCGCCGGCTTGTCGAGACCACTGCATTCGCGCG
GGTACGACCGCGCCACCAGC

Downstream 100 bases:

>100_bases
GACGACAGCCGCGGAAGGATAGCGCCGGCTCAGGGCGTCTGGGCTGGTGCTGCGTTATCGCTGCCAGCCACGGCCTTGAC
GCCGGCCTTGGTCACGTCGT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNQPLNPESLGIDTLGVRAGTLRSEYMEHSEAMYLTSSFCFHSAAEAAERFANSEEGFTYSRFTNPTVSMFQSRLAALEG
AESCMATASGMSAILSVVMSLMQAGDHLVSSRAIFGSTMTLFGNIFSKFGVETTFVDPGDLNAWRAAVRPTTKLFFLETP
SNPLTEVADIAAVADIAHNAGAQLIVDNCFCSPALQQPIKFGADIVVHSATKHIDGQGRVLGGAVVGSHDFIMGKVFPFV
RTAGPTLSAFNAWVLLKGMETLAIRMERHSANALALAEFLESHPAVARVFHPALKSHPQHEIAMRQQSGGGAIVSFELKG
ATPEQQRANAWRVIDNTRLCSITGNLGDTRTTITHPYTTTHARVSPEAKAAAGVGEGLIRLAVGLETVADLKADLLRGLG
E

Sequences:

>Translated_401_residues
MNQPLNPESLGIDTLGVRAGTLRSEYMEHSEAMYLTSSFCFHSAAEAAERFANSEEGFTYSRFTNPTVSMFQSRLAALEG
AESCMATASGMSAILSVVMSLMQAGDHLVSSRAIFGSTMTLFGNIFSKFGVETTFVDPGDLNAWRAAVRPTTKLFFLETP
SNPLTEVADIAAVADIAHNAGAQLIVDNCFCSPALQQPIKFGADIVVHSATKHIDGQGRVLGGAVVGSHDFIMGKVFPFV
RTAGPTLSAFNAWVLLKGMETLAIRMERHSANALALAEFLESHPAVARVFHPALKSHPQHEIAMRQQSGGGAIVSFELKG
ATPEQQRANAWRVIDNTRLCSITGNLGDTRTTITHPYTTTHARVSPEAKAAAGVGEGLIRLAVGLETVADLKADLLRGLG
E
>Mature_401_residues
MNQPLNPESLGIDTLGVRAGTLRSEYMEHSEAMYLTSSFCFHSAAEAAERFANSEEGFTYSRFTNPTVSMFQSRLAALEG
AESCMATASGMSAILSVVMSLMQAGDHLVSSRAIFGSTMTLFGNIFSKFGVETTFVDPGDLNAWRAAVRPTTKLFFLETP
SNPLTEVADIAAVADIAHNAGAQLIVDNCFCSPALQQPIKFGADIVVHSATKHIDGQGRVLGGAVVGSHDFIMGKVFPFV
RTAGPTLSAFNAWVLLKGMETLAIRMERHSANALALAEFLESHPAVARVFHPALKSHPQHEIAMRQQSGGGAIVSFELKG
ATPEQQRANAWRVIDNTRLCSITGNLGDTRTTITHPYTTTHARVSPEAKAAAGVGEGLIRLAVGLETVADLKADLLRGLG
E

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=329, Percent_Identity=37.3860182370821, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI299473759, Length=329, Percent_Identity=34.3465045592705, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI25453487, Length=328, Percent_Identity=31.0975609756098, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=37.984496124031, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789383, Length=367, Percent_Identity=28.8828337874659, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=334, Percent_Identity=38.3233532934132, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=38.2530120481928, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71980708, Length=368, Percent_Identity=33.4239130434783, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=403, Percent_Identity=33.7468982630273, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6319307, Length=358, Percent_Identity=35.4748603351955, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=25.8454106280193, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=291, Percent_Identity=28.1786941580756, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=248, Percent_Identity=31.0483870967742, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922554, Length=346, Percent_Identity=34.971098265896, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42836; Mature: 42836

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPLNPESLGIDTLGVRAGTLRSEYMEHSEAMYLTSSFCFHSAAEAAERFANSEEGFTY
CCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCEEE
SRFTNPTVSMFQSRLAALEGAESCMATASGMSAILSVVMSLMQAGDHLVSSRAIFGSTMT
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LFGNIFSKFGVETTFVDPGDLNAWRAAVRPTTKLFFLETPSNPLTEVADIAAVADIAHNA
HHHHHHHHHCCEEEEECCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCC
GAQLIVDNCFCSPALQQPIKFGADIVVHSATKHIDGQGRVLGGAVVGSHDFIMGKVFPFV
CCEEEEECCCCCHHHHHHHHCCCCEEEECHHHCCCCCCEEEEEEEECCCCHHHHHHHHHH
RTAGPTLSAFNAWVLLKGMETLAIRMERHSANALALAEFLESHPAVARVFHPALKSHPQH
HCCCCCHHHHHHHHHHHCHHHHHEEHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCH
EIAMRQQSGGGAIVSFELKGATPEQQRANAWRVIDNTRLCSITGNLGDTRTTITHPYTTT
HHHHHCCCCCCEEEEEEECCCCCHHHHCCCEEEECCCEEEEEECCCCCCCCEEECCCCCC
HARVSPEAKAAAGVGEGLIRLAVGLETVADLKADLLRGLGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQPLNPESLGIDTLGVRAGTLRSEYMEHSEAMYLTSSFCFHSAAEAAERFANSEEGFTY
CCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCEEE
SRFTNPTVSMFQSRLAALEGAESCMATASGMSAILSVVMSLMQAGDHLVSSRAIFGSTMT
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LFGNIFSKFGVETTFVDPGDLNAWRAAVRPTTKLFFLETPSNPLTEVADIAAVADIAHNA
HHHHHHHHHCCEEEEECCCCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCC
GAQLIVDNCFCSPALQQPIKFGADIVVHSATKHIDGQGRVLGGAVVGSHDFIMGKVFPFV
CCEEEEECCCCCHHHHHHHHCCCCEEEECHHHCCCCCCEEEEEEEECCCCHHHHHHHHHH
RTAGPTLSAFNAWVLLKGMETLAIRMERHSANALALAEFLESHPAVARVFHPALKSHPQH
HCCCCCHHHHHHHHHHHCHHHHHEEHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCH
EIAMRQQSGGGAIVSFELKGATPEQQRANAWRVIDNTRLCSITGNLGDTRTTITHPYTTT
HHHHHCCCCCCEEEEEEECCCCCHHHHCCCEEEECCCEEEEEECCCCCCCCEEECCCCCC
HARVSPEAKAAAGVGEGLIRLAVGLETVADLKADLLRGLGE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]