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The map label for this gene is pdxJ [H]

Identifier: 73541936

GI number: 73541936

Start: 2482086

End: 2482868

Strand: Reverse

Name: pdxJ [H]

Synonym: Reut_A2249

Alternate gene names: 73541936

Gene position: 2482868-2482086 (Counterclockwise)

Preceding gene: 73541937

Following gene: 73541935

Centisome position: 65.23

GC content: 67.05

Gene sequence:

>783_bases
ATGATCTTCCACGCAAATCCGGGCGTCATCGACCTCGGCATCAACATCGACCACGTCGCCACGCTGCGCAATGCGCGCGG
CACGGTGTATCCCGACCCGATCGAGGCAGCATTGCAGGCCGAAGAAGCCGGCGCGGACCTGATCACGCTGCACCTGCGCG
AAGACCGCCGCCATATCCGCGATGCCGATGTGCGCGCGCTGCGCCCACAACTCGCCACGCGCATGAACCTGGAATGCGCC
ATCACGCAGGAGATGCTCGACATCGCCTGCGAGATCCGGCCGCAGGACGTCTGCCTCGTGCCCGAGCGGCGCGAGGAGGT
CACGACCGAAGGCGGGCTCGATGTGGCAGGTCGCTTTGGACAGGTCAGTTCCGCTTGCAAGCAGCTCGCCGCAGCCGGCA
TCCGTGTATCGCTGTTCATTGACGCCGATGCCGACCAGATCGCCGCCGCTGCGGCCTGCGGTGCGCCGGTGATCGAACTG
CATACCGGCCGCTATGCCGATGCGCATACGCCTGACGAGCTGGCCATCGAATTCCGCCGCGTTGCCGACGGCGTCGATGC
CGGCATCCGCCACGGCCTGGTCGTGAACGCGGGCCACGGCCTGCATTACACGAACGTGCAGCCGATTGCGGCGCTGGCGG
GCATCAAGGAACTCAACATCGGCCACGCGGTCGTCGCGCATGCCGTGTTCGTGGGCTGGCAAAACGCCGTGCGCGAGATG
AAGGCCATCATGGTGGCCGCGCGCCTCGGCACCCGTTACCCCGCTGCAGGCAAGCCGGCGTGA

Upstream 100 bases:

>100_bases
TATTATTTGCAGGGAGCGCCGCTCAAGACGCGGCAGATCCTGATCGACCTGCACTATCTCTGACCCGCCGGGCAGCCGGC
CCACCGCTGATTCCGCAAGC

Downstream 100 bases:

>100_bases
TCTACGGCGTCGGCACCGACATCATCGAGATCGCGCGCGTGCAGGGCGTGATGGAGCGCACGCGCGGCCGCTTTGCCGAG
AAGGTGCTCGGGGCCGATGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MIFHANPGVIDLGINIDHVATLRNARGTVYPDPIEAALQAEEAGADLITLHLREDRRHIRDADVRALRPQLATRMNLECA
ITQEMLDIACEIRPQDVCLVPERREEVTTEGGLDVAGRFGQVSSACKQLAAAGIRVSLFIDADADQIAAAAACGAPVIEL
HTGRYADAHTPDELAIEFRRVADGVDAGIRHGLVVNAGHGLHYTNVQPIAALAGIKELNIGHAVVAHAVFVGWQNAVREM
KAIMVAARLGTRYPAAGKPA

Sequences:

>Translated_260_residues
MIFHANPGVIDLGINIDHVATLRNARGTVYPDPIEAALQAEEAGADLITLHLREDRRHIRDADVRALRPQLATRMNLECA
ITQEMLDIACEIRPQDVCLVPERREEVTTEGGLDVAGRFGQVSSACKQLAAAGIRVSLFIDADADQIAAAAACGAPVIEL
HTGRYADAHTPDELAIEFRRVADGVDAGIRHGLVVNAGHGLHYTNVQPIAALAGIKELNIGHAVVAHAVFVGWQNAVREM
KAIMVAARLGTRYPAAGKPA
>Mature_260_residues
MIFHANPGVIDLGINIDHVATLRNARGTVYPDPIEAALQAEEAGADLITLHLREDRRHIRDADVRALRPQLATRMNLECA
ITQEMLDIACEIRPQDVCLVPERREEVTTEGGLDVAGRFGQVSSACKQLAAAGIRVSLFIDADADQIAAAAACGAPVIEL
HTGRYADAHTPDELAIEFRRVADGVDAGIRHGLVVNAGHGLHYTNVQPIAALAGIKELNIGHAVVAHAVFVGWQNAVREM
KAIMVAARLGTRYPAAGKPA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=237, Percent_Identity=63.2911392405063, Blast_Score=291, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 27813; Mature: 27813

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFHANPGVIDLGINIDHVATLRNARGTVYPDPIEAALQAEEAGADLITLHLREDRRHIR
CEEECCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHH
DADVRALRPQLATRMNLECAITQEMLDIACEIRPQDVCLVPERREEVTTEGGLDVAGRFG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCHHCCHH
QVSSACKQLAAAGIRVSLFIDADADQIAAAAACGAPVIELHTGRYADAHTPDELAIEFRR
HHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
VADGVDAGIRHGLVVNAGHGLHYTNVQPIAALAGIKELNIGHAVVAHAVFVGWQNAVREM
HHHHHHHHHHCCEEEECCCCEEECCCCHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHH
KAIMVAARLGTRYPAAGKPA
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIFHANPGVIDLGINIDHVATLRNARGTVYPDPIEAALQAEEAGADLITLHLREDRRHIR
CEEECCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHH
DADVRALRPQLATRMNLECAITQEMLDIACEIRPQDVCLVPERREEVTTEGGLDVAGRFG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCHHCCHH
QVSSACKQLAAAGIRVSLFIDADADQIAAAAACGAPVIELHTGRYADAHTPDELAIEFRR
HHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
VADGVDAGIRHGLVVNAGHGLHYTNVQPIAALAGIKELNIGHAVVAHAVFVGWQNAVREM
HHHHHHHHHHCCEEEECCCCEEECCCCHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHH
KAIMVAARLGTRYPAAGKPA
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA