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The map label for this gene is rpoS [H]

Identifier: 73541783

GI number: 73541783

Start: 2302978

End: 2304153

Strand: Reverse

Name: rpoS [H]

Synonym: Reut_A2095

Alternate gene names: 73541783

Gene position: 2304153-2302978 (Counterclockwise)

Preceding gene: 73541784

Following gene: 73541782

Centisome position: 60.53

GC content: 61.9

Gene sequence:

>1176_bases
ATGCCACGCCAGAAAACTGTCTCCTCCGGAACCGTCAGCAGGGCTCGCCGTCCCAAGCAGCCGGAAGTGAAGGCTGGTGT
GGCCCAAACCGACGATCGCGTTGAACTGGATGCATTCGACAAGGATGTCGGTGCCGACCTGATTCCCCCGACCGACGAGC
CGATCTCGACCACGACAACGGTCGGCCTGCGCGAAGCCGATTTGCTCAGCGACGACAACGACCGTCAGCTGCGCGATGAT
GACGAGGATGAGGCAGAGGAGGAAGAGGAATCCGAAAGCGAGAACGTCGCCGAAGCGGCGCCCGAGCATGACGACTTCCG
TACCGTGCTGCATACCGAGCTGGCTGCCGATACGGTCCAGCACTATCTGAATCGCATCAGCATCAAGCCGCTGCTGTCCG
CTCCTGAGGAGTTGCATTTCTCGACGCTGGCCAAGGACGGCGATTTTTCCGCGCGGCAGGTCATGATCGAACGCAACCTG
CGCCTGGTCGTGAGCATCGCCAAGGGTTATCTCAATCGCGGCGTGCCGCTGCTGGACCTGATCGAGGAAGGCAACCTTGG
TCTCATGCACGCGATCGAGAAATTCGATCCGTCACGCGGTTTCCGTTTTTCTACCTACGCGACCTGGTGGATCCGCCAGA
GCATCGAGCGCGCCATCATGAACCAGGCGCGCACGGTACGGCTGCCGGTCCACGTGATCCGTGAACTCAACCAGGTGCTG
CGCGCCAAGCGGCATCTCGAAAAAGGCGGCACCGACGGCCGCGACGCCAGTCTCGAAGACATTGCCCACCTGCTGGGCAA
GACGCCTGACGAGGTGCAGGACGTGCTGGCGCTTAACGAACATACCACCTCGCTCGATACGCCGTTTGACCTGGACCCGG
GCTCCAGCCTGCTGGACTTCCTTTCCGACGAGCACAACGCCGCCCCGGACCAGGAAGTCGCGCACCGCGAGCTCGAAGGC
CTGATGAAGCTGTGGCTGGCCCGCCTGTCGGAAAAGCACCGCTATGTGGTGGAGCGCCGCTTCGGCCTCAACCATATCGA
ACCGGCCACGCTTGAAGAGCTGGCCGAAGAGATGGGCCTGACGCGCGAACGCGTGCGCCAGATCCAACAGGAAGCCCTGG
TCAAGCTCAAGCGGCATTTCGCATCGCAAGGTGTCAGGAAGGACGCTGTTCTATGA

Upstream 100 bases:

>100_bases
GGGCAATAGTGATACCGACCGGGTAAAGCTTCACTTCGAAGTTCGCAAGAACGGAAAGCCGGTTGATCCGATGCGGTATC
TGCCACCACAGTGAGGGTTC

Downstream 100 bases:

>100_bases
CCCCTGTGCTGGTATTCGACATCGAGACCATTCCCGATGTCGACGGCCTGCGCCGTTTGCATGACCATCCCGCATCGATG
AGCGACGAGGAAGTCGCCGA

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPRQKTVSSGTVSRARRPKQPEVKAGVAQTDDRVELDAFDKDVGADLIPPTDEPISTTTTVGLREADLLSDDNDRQLRDD
DEDEAEEEEESESENVAEAAPEHDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKDGDFSARQVMIERNL
RLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVL
RAKRHLEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELEG
LMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Sequences:

>Translated_391_residues
MPRQKTVSSGTVSRARRPKQPEVKAGVAQTDDRVELDAFDKDVGADLIPPTDEPISTTTTVGLREADLLSDDNDRQLRDD
DEDEAEEEEESESENVAEAAPEHDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKDGDFSARQVMIERNL
RLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVL
RAKRHLEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELEG
LMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL
>Mature_390_residues
PRQKTVSSGTVSRARRPKQPEVKAGVAQTDDRVELDAFDKDVGADLIPPTDEPISTTTTVGLREADLLSDDNDRQLRDDD
EDEAEEEEESESENVAEAAPEHDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKDGDFSARQVMIERNLR
LVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLR
AKRHLEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELEGL
MKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=280, Percent_Identity=51.7857142857143, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1789448, Length=236, Percent_Identity=41.9491525423729, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=29.7101449275362, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 44214; Mature: 44083

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQKTVSSGTVSRARRPKQPEVKAGVAQTDDRVELDAFDKDVGADLIPPTDEPISTTTT
CCCCCCCCCCCHHHHCCCCCCCHHCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCHHH
VGLREADLLSDDNDRQLRDDDEDEAEEEEESESENVAEAAPEHDDFRTVLHTELAADTVQ
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HYLNRISIKPLLSAPEELHFSTLAKDGDFSARQVMIERNLRLVVSIAKGYLNRGVPLLDL
HHHHHCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHH
IEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVL
HHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RAKRHLEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDF
HHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LSDEHNAAPDQEVAHRELEGLMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
TRERVRQIQQEALVKLKRHFASQGVRKDAVL
CHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
PRQKTVSSGTVSRARRPKQPEVKAGVAQTDDRVELDAFDKDVGADLIPPTDEPISTTTT
CCCCCCCCCCHHHHCCCCCCCHHCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCHHH
VGLREADLLSDDNDRQLRDDDEDEAEEEEESESENVAEAAPEHDDFRTVLHTELAADTVQ
CCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HYLNRISIKPLLSAPEELHFSTLAKDGDFSARQVMIERNLRLVVSIAKGYLNRGVPLLDL
HHHHHCCCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHH
IEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVL
HHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHH
RAKRHLEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDF
HHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
LSDEHNAAPDQEVAHRELEGLMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
TRERVRQIQQEALVKLKRHFASQGVRKDAVL
CHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]