Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 73541758

GI number: 73541758

Start: 2274959

End: 2275741

Strand: Reverse

Name: cobB [H]

Synonym: Reut_A2070

Alternate gene names: 73541758

Gene position: 2275741-2274959 (Counterclockwise)

Preceding gene: 73541759

Following gene: 73541756

Centisome position: 59.79

GC content: 67.05

Gene sequence:

>783_bases
ATGAACGATGATGCCCTTATCCCCCTGTCGTCGCCGTGGCCCGGCCTGGAAGAAGCCCGGGAACTGATTGCCCAGGCTCG
CAGCGTGTTCGTGCTGACCGGGGCAGGCATCTCCGCGGAATCCGGCGTGCCGACGTTCCGGGATGCGCTGACCGGTCTCT
GGGCACGTTTTGATCCCGAGGAGCTGGCGAGCGAAGAGGCATACCGCCGCCAACCGGCACTGGTGTGGGAGTGGTATCAG
CATCGCCGCGACCTCGTGGCTGCCGCACGGCCGAATCCTGCGCACTATGCGCTGGTGGCTTTGGCCGCGCAGAAGACGGT
GACGCTGGTCACGCAGAATGTGGACGGGCTGCACCAGCGCGCCGGCAGCGATCATGTGATCGAGCTGCATGGAAACCTGT
TCGCGAACAAATGGCTGAACGGCTGCGGGCGCTGCGATGAGGCCACGGCGATTCCCGGCGATCCGCCACGCTGCAGCCTG
TGTGGTGCGCTGATGCGCCCCGGGGTGGTGTGGTTCGGGGAAGACCTGCCGCGTGTGGCGCGTTTCCGCGCCGAACATGC
CGCCGAGACGGCGGACCTGTGCCTCGTCGTGGGGACGTCGGGGTTGGTGTACCCGGCAGCCGGGCTGCCGGGTTTGGCCA
GGGATCACGGCGCACGGGTCGTCGTGGTGAATCCGCAGCCCTCGGTGCTGGATCAGACCGCTGATGTGGTACTGCAGGCA
GCAGCAGGTGCAAGCCTGCCGCTGCTATGGCCGCAGTCCGGCTCCGATACGGAGATGGACTGA

Upstream 100 bases:

>100_bases
GTTGCCGCCGTGCCATGCCTGTCCGACTTCCTGAAGGCGATTGCTATGCAGTGCATTGCAGATATCCCGCAATCTCGGCC
GACAGGCGGGAAATGACACC

Downstream 100 bases:

>100_bases
GCGGCCGCCTCGGTCGGCGCCTTAGCCGGCGATTTCCGCAACCAGCTTTTCCAGCTTGATTGCGTCCGCAGCAAACAGAC
GGATGCCTTCAGCGAGTTTC

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNDDALIPLSSPWPGLEEARELIAQARSVFVLTGAGISAESGVPTFRDALTGLWARFDPEELASEEAYRRQPALVWEWYQ
HRRDLVAAARPNPAHYALVALAAQKTVTLVTQNVDGLHQRAGSDHVIELHGNLFANKWLNGCGRCDEATAIPGDPPRCSL
CGALMRPGVVWFGEDLPRVARFRAEHAAETADLCLVVGTSGLVYPAAGLPGLARDHGARVVVVNPQPSVLDQTADVVLQA
AAGASLPLLWPQSGSDTEMD

Sequences:

>Translated_260_residues
MNDDALIPLSSPWPGLEEARELIAQARSVFVLTGAGISAESGVPTFRDALTGLWARFDPEELASEEAYRRQPALVWEWYQ
HRRDLVAAARPNPAHYALVALAAQKTVTLVTQNVDGLHQRAGSDHVIELHGNLFANKWLNGCGRCDEATAIPGDPPRCSL
CGALMRPGVVWFGEDLPRVARFRAEHAAETADLCLVVGTSGLVYPAAGLPGLARDHGARVVVVNPQPSVLDQTADVVLQA
AAGASLPLLWPQSGSDTEMD
>Mature_260_residues
MNDDALIPLSSPWPGLEEARELIAQARSVFVLTGAGISAESGVPTFRDALTGLWARFDPEELASEEAYRRQPALVWEWYQ
HRRDLVAAARPNPAHYALVALAAQKTVTLVTQNVDGLHQRAGSDHVIELHGNLFANKWLNGCGRCDEATAIPGDPPRCSL
CGALMRPGVVWFGEDLPRVARFRAEHAAETADLCLVVGTSGLVYPAAGLPGLARDHGARVVVVNPQPSVLDQTADVVLQA
AAGASLPLLWPQSGSDTEMD

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=256, Percent_Identity=33.59375, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI6912664, Length=274, Percent_Identity=32.4817518248175, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI13787215, Length=234, Percent_Identity=35.042735042735, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI6912662, Length=278, Percent_Identity=31.6546762589928, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI7657575, Length=232, Percent_Identity=29.7413793103448, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI300797577, Length=243, Percent_Identity=30.4526748971193, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI6912660, Length=224, Percent_Identity=33.4821428571429, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI63054862, Length=205, Percent_Identity=32.6829268292683, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI13775602, Length=220, Percent_Identity=30, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI13775600, Length=207, Percent_Identity=30.9178743961353, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI300797705, Length=198, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI308199517, Length=205, Percent_Identity=43.4146341463415, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17567771, Length=264, Percent_Identity=28.7878787878788, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17541892, Length=212, Percent_Identity=28.3018867924528, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71990482, Length=267, Percent_Identity=28.8389513108614, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990487, Length=269, Percent_Identity=28.6245353159851, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325242, Length=227, Percent_Identity=31.2775330396476, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28571445, Length=279, Percent_Identity=29.0322580645161, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648389, Length=197, Percent_Identity=29.4416243654822, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24645650, Length=251, Percent_Identity=26.6932270916335, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137536, Length=234, Percent_Identity=26.4957264957265, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27882; Mature: 27882

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDDALIPLSSPWPGLEEARELIAQARSVFVLTGAGISAESGVPTFRDALTGLWARFDPE
CCCCCEEECCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHH
ELASEEAYRRQPALVWEWYQHRRDLVAAARPNPAHYALVALAAQKTVTLVTQNVDGLHQR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCHHHHHH
AGSDHVIELHGNLFANKWLNGCGRCDEATAIPGDPPRCSLCGALMRPGVVWFGEDLPRVA
CCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHH
RFRAEHAAETADLCLVVGTSGLVYPAAGLPGLARDHGARVVVVNPQPSVLDQTADVVLQA
HHHHHHHCCCCCEEEEECCCCCEECCCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHH
AAGASLPLLWPQSGSDTEMD
HCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MNDDALIPLSSPWPGLEEARELIAQARSVFVLTGAGISAESGVPTFRDALTGLWARFDPE
CCCCCEEECCCCCCCHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHH
ELASEEAYRRQPALVWEWYQHRRDLVAAARPNPAHYALVALAAQKTVTLVTQNVDGLHQR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCHHHHHH
AGSDHVIELHGNLFANKWLNGCGRCDEATAIPGDPPRCSLCGALMRPGVVWFGEDLPRVA
CCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHH
RFRAEHAAETADLCLVVGTSGLVYPAAGLPGLARDHGARVVVVNPQPSVLDQTADVVLQA
HHHHHHHCCCCCEEEEECCCCCEECCCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHH
AAGASLPLLWPQSGSDTEMD
HCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]