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The map label for this gene is rlmB [H]

Identifier: 73541759

GI number: 73541759

Start: 2275970

End: 2276740

Strand: Reverse

Name: rlmB [H]

Synonym: Reut_A2071

Alternate gene names: 73541759

Gene position: 2276740-2275970 (Counterclockwise)

Preceding gene: 73541760

Following gene: 73541758

Centisome position: 59.81

GC content: 65.76

Gene sequence:

>771_bases
ATGGCCGGGCCGGGCGAGTACAATCGCGCCATGGCTAAACAAAAACTTCTGATCGGCTTTCACGCCGTCACCGCGCGCTT
GCGCCAGGACGCCAAGGGCGTCACCGATGTCTATGTCGAGGCTTCGCGCCGCGACCGTCGCATGCAGGACTTCATCCGCC
TGGCCGAGAGCCTCGGTGTGCGCCTGCACCCCGTTGATGCCGAACGCTTGCGCGGCATGGCGGGCACCGACCGTCATCAG
GGCGTGGTGGCGCGTGCTGAAGACGTGGCGCTTGCACTCAATCTCGACGAATTGCTCGATGGTATTGAGGGCACGCCGCT
GCTGCTCGTGCTCGACGGTGTGACGGATCCGCATAACCTCGGCGCCTGCCTGCGTGTGGCGGACGGCGCCGGCGCGCATG
CGGTGATCGCGCCCAAGGACCGCAGCGTCGGACTGAATACAACCGTGGCCAAGGTGGCGAGCGGCGCGGCCGAGACCGTG
CCTTACATCACCGTGACCAATCTTGCCCGCACGCTGCGTGAACTGCAGGAACGGGGCATCTGGGTGGTCGGCACGGCCGA
CGGTACTGACAAGACGCTTTACGATGTGGACCTGAAGGGCCCGATGGCGATCGTCATGGGAGCCGAGGGCGAGGGCATGC
GCCGCCTGACGCGCGAGACCTGCGACGAACTGGTTGGCATCCCCATGCTTGGCGGTGTCGAAAGCCTGAACGTGTCGGTG
GCCAGCGGTGTCTGCCTGTACGAAGCGGTGCGCCAGCGGCGCTTGAAGTAA

Upstream 100 bases:

>100_bases
AAGACCGCAGGCAAAACCTCGCGCACGCCGCGCAAGCGTTGAGCCTGAGCCGCTCCGGGGCGCCTCCACATCGGCGCCCC
GGACGGCACGCACAAGCGTG

Downstream 100 bases:

>100_bases
AGCATCCTTGTATCTGCTGCCGCTGTAACGCGGCACGTTGCCGGCCGTCCGCGCTGTCGCCGTCGCACGGGGCTAGAATC
TGTGTCATCCCCTGGCCTGG

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAGPGEYNRAMAKQKLLIGFHAVTARLRQDAKGVTDVYVEASRRDRRMQDFIRLAESLGVRLHPVDAERLRGMAGTDRHQ
GVVARAEDVALALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDRSVGLNTTVAKVASGAAETV
PYITVTNLARTLRELQERGIWVVGTADGTDKTLYDVDLKGPMAIVMGAEGEGMRRLTRETCDELVGIPMLGGVESLNVSV
ASGVCLYEAVRQRRLK

Sequences:

>Translated_256_residues
MAGPGEYNRAMAKQKLLIGFHAVTARLRQDAKGVTDVYVEASRRDRRMQDFIRLAESLGVRLHPVDAERLRGMAGTDRHQ
GVVARAEDVALALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDRSVGLNTTVAKVASGAAETV
PYITVTNLARTLRELQERGIWVVGTADGTDKTLYDVDLKGPMAIVMGAEGEGMRRLTRETCDELVGIPMLGGVESLNVSV
ASGVCLYEAVRQRRLK
>Mature_255_residues
AGPGEYNRAMAKQKLLIGFHAVTARLRQDAKGVTDVYVEASRRDRRMQDFIRLAESLGVRLHPVDAERLRGMAGTDRHQG
VVARAEDVALALNLDELLDGIEGTPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDRSVGLNTTVAKVASGAAETVP
YITVTNLARTLRELQERGIWVVGTADGTDKTLYDVDLKGPMAIVMGAEGEGMRRLTRETCDELVGIPMLGGVESLNVSVA
SGVCLYEAVRQRRLK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=54.356846473029, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1788935, Length=208, Percent_Identity=29.3269230769231, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790083, Length=151, Percent_Identity=33.7748344370861, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17555908, Length=256, Percent_Identity=24.609375, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=166, Percent_Identity=33.7349397590361, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27477; Mature: 27346

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGPGEYNRAMAKQKLLIGFHAVTARLRQDAKGVTDVYVEASRRDRRMQDFIRLAESLGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECHHHHHHHHHHHHHHHHCCC
RLHPVDAERLRGMAGTDRHQGVVARAEDVALALNLDELLDGIEGTPLLLVLDGVTDPHNL
EEECCCHHHHCCCCCCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEEEECCCCCCCC
GACLRVADGAGAHAVIAPKDRSVGLNTTVAKVASGAAETVPYITVTNLARTLRELQERGI
CCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCE
WVVGTADGTDKTLYDVDLKGPMAIVMGAEGEGMRRLTRETCDELVGIPMLGGVESLNVSV
EEEECCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
ASGVCLYEAVRQRRLK
HCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AGPGEYNRAMAKQKLLIGFHAVTARLRQDAKGVTDVYVEASRRDRRMQDFIRLAESLGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECHHHHHHHHHHHHHHHHCCC
RLHPVDAERLRGMAGTDRHQGVVARAEDVALALNLDELLDGIEGTPLLLVLDGVTDPHNL
EEECCCHHHHCCCCCCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEEEECCCCCCCC
GACLRVADGAGAHAVIAPKDRSVGLNTTVAKVASGAAETVPYITVTNLARTLRELQERGI
CCEEEEECCCCCEEEECCCCCCCCCCHHHHHHHCCCHHHCCEEEHHHHHHHHHHHHHCCE
WVVGTADGTDKTLYDVDLKGPMAIVMGAEGEGMRRLTRETCDELVGIPMLGGVESLNVSV
EEEECCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
ASGVCLYEAVRQRRLK
HCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]