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The map label for this gene is ygbJ [C]

Identifier: 73541121

GI number: 73541121

Start: 1543815

End: 1544708

Strand: Reverse

Name: ygbJ [C]

Synonym: Reut_A1427

Alternate gene names: 73541121

Gene position: 1544708-1543815 (Counterclockwise)

Preceding gene: 73541122

Following gene: 73541120

Centisome position: 40.58

GC content: 67.23

Gene sequence:

>894_bases
ATGTCCCGGAATATCGGCGTAATCGGCCTTGGCGCCATGGGCAACGGTGTCGCGCAGTCGCTGCTGCGAGCCGGATTTAC
CGTGCACGCCTGCGACCTTCGGCCCGACGTGCTGCAGCGTTTTGCCGACGCCGGCGGCGTGCCGTGCGCAAGCCCCGCCG
AACTGGGCAGCCGCTGCGATGTCGTGATCACGCTGGTAGTCAACGCCCAGCAGACAGAGGCCGTTCTGTTCGGTGCCGAC
GGTGCCGCCACGACGATGCACTCCGGCACGCTGGTGATCGCGAGCGCGACCGTGCCGCCCTCGTTTGCCGAAGCGCTGGG
CCGTCGCCTGGGCGAGCAAGGCATCCTTCTGCTCGATGCCCCGGTGTCCGGGGGCGCGGCGCGGGCGGCCAGCGGCGAGA
TGACCATGATGACGTCCGGACCCGCCGAAGCGTATTCGCTGGCCGAGGACGTGCTGGCCGCGATCTCCGGCAAGGTCTAC
CGCCTCGGCAACGCGCATGGTGCCGGCTCCAAGGTCAAAATCATCAACCAGCTTCTGGCGGGCGTGCATATCGCCGCCGC
GGCGGAGGCCATGGCACTGGGCCTGCGCGAAGGTGTGGACCCGGATGCGCTGTACGACGTCATCACCCATAGCGCCGGCA
ATTCGTGGATGTTCGAGAACCGCGTGCCGCATATCCTGTCGGGCGACTACACGCCGCTGTCCGCAGTCGATATCTTCGTC
AAGGACCTCGGCATGGTGCTCGATACCGCGCGCACCAGCAAATTCCCGCTGCCGCTGTCCGCCGCCGCGCACCAGATGTT
CATGATGGCCTCCACCGCCGGCCACGGCGGCGAAGACGATTCCGCCGTGATCAAGATCTTCCCCGGCATCGAACTGCCGG
CCAAGGCAGAGTGA

Upstream 100 bases:

>100_bases
GCTCGGCCAGGACGAAGCGGACGACGAGCGTCCCGGCACCGCCAGCTAAGCCGGCGCCGCCCTATCGATTGACCTGCCTT
ACCTGCATCAGGAGTTTTCT

Downstream 100 bases:

>100_bases
GGCGAAGCACGTCATGACCGCCATCCAGCAACAAGATCGCCCGCTGCTCGGCTGCATCGCCGACGACTTCACCGGCGCCA
CCGACCTCGCCAATACGCTC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSRNIGVIGLGAMGNGVAQSLLRAGFTVHACDLRPDVLQRFADAGGVPCASPAELGSRCDVVITLVVNAQQTEAVLFGAD
GAATTMHSGTLVIASATVPPSFAEALGRRLGEQGILLLDAPVSGGAARAASGEMTMMTSGPAEAYSLAEDVLAAISGKVY
RLGNAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVDPDALYDVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFV
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIELPAKAE

Sequences:

>Translated_297_residues
MSRNIGVIGLGAMGNGVAQSLLRAGFTVHACDLRPDVLQRFADAGGVPCASPAELGSRCDVVITLVVNAQQTEAVLFGAD
GAATTMHSGTLVIASATVPPSFAEALGRRLGEQGILLLDAPVSGGAARAASGEMTMMTSGPAEAYSLAEDVLAAISGKVY
RLGNAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVDPDALYDVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFV
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIELPAKAE
>Mature_296_residues
SRNIGVIGLGAMGNGVAQSLLRAGFTVHACDLRPDVLQRFADAGGVPCASPAELGSRCDVVITLVVNAQQTEAVLFGADG
AATTMHSGTLVIASATVPPSFAEALGRRLGEQGILLLDAPVSGGAARAASGEMTMMTSGPAEAYSLAEDVLAAISGKVYR
LGNAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVDPDALYDVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVK
DLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIELPAKAE

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=32.7586206896552, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=24.5614035087719, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=52.5773195876289, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=31.0714285714286, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=30.8510638297872, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=26.241134751773, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=29.2592592592593, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=29.8305084745763, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=29.8305084745763, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=23.6749116607774, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 30406; Mature: 30275

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNIGVIGLGAMGNGVAQSLLRAGFTVHACDLRPDVLQRFADAGGVPCASPAELGSRCD
CCCCCCEEEECCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHCCCCC
VVITLVVNAQQTEAVLFGADGAATTMHSGTLVIASATVPPSFAEALGRRLGEQGILLLDA
EEEEEEEECCCCCEEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEC
PVSGGAARAASGEMTMMTSGPAEAYSLAEDVLAAISGKVYRLGNAHGAGSKVKIINQLLA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHH
GVHIAAAAEAMALGLREGVDPDALYDVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHHCCCCCCHHHHHHHH
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIELPAKAE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SRNIGVIGLGAMGNGVAQSLLRAGFTVHACDLRPDVLQRFADAGGVPCASPAELGSRCD
CCCCCEEEECCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHCCCCC
VVITLVVNAQQTEAVLFGADGAATTMHSGTLVIASATVPPSFAEALGRRLGEQGILLLDA
EEEEEEEECCCCCEEEEECCCCCEEECCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEC
PVSGGAARAASGEMTMMTSGPAEAYSLAEDVLAAISGKVYRLGNAHGAGSKVKIINQLLA
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHH
GVHIAAAAEAMALGLREGVDPDALYDVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHHCCCCCCHHHHHHHH
KDLGMVLDTARTSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIELPAKAE
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]