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The map label for this gene is lhgO [H]

Identifier: 73540954

GI number: 73540954

Start: 1344332

End: 1345438

Strand: Reverse

Name: lhgO [H]

Synonym: Reut_A1259

Alternate gene names: 73540954

Gene position: 1345438-1344332 (Counterclockwise)

Preceding gene: 73540955

Following gene: 73540948

Centisome position: 35.35

GC content: 67.21

Gene sequence:

>1107_bases
ATGGAGAACGTCGATTGCGTGGTGATCGGTGCAGGCGTCGTCGGGCTGGCTGTCGCCAGGGCGCTGGCGCAGAACGGGCG
CGAAGTGATCATCCTGGAGGCCGAGAATGCGTTCGGCACCATTACCAGCGCGCGCAACAGCGAGGTGATCCATGCCGGCA
TCTACTATCCGGCCGGCTCGCTCAAGGCAGAACTATGCGTCCGCGGCAAGGAGATGCTGTACGAGTACTGCGCCTCGCAC
CACGTTGCCCATCAGCGTTGCGGCAAGCTGATCGTCGCCACGAGCGAGGCGCAGGTCGCCACGCTGGAGGGCATCCGAGC
GAAGGCCGCGGCCAATGGCGTGCATGACATGCAGTTGCTGACCCGAGCCGAAGCGCAGGCCCTGGAGCCGCAACTGCAAT
GCCACGCCGCCCTGCTCTCGCCATCGACCGGCATCGTGGACAGCCACGGCTTGATGACAGCGCTGCTCGGCGACGCCGAG
AACGCGGGCGCGATGCTGGCCGTACAGTCGCCGGTGCGGCGCGGCGCGATCACGCCCGCAGGCATCGAGCTTGAAGTCGG
CAGCGAGGACGGCGGCAGCACCACGCTGCTGGCCCGCTCGGTCGTCAACTCAGCCGGCCTGACCGCGCCCGAACTGGCTC
GGCGTATCGACGGCATGCCGGAAGACCACATCCCGCCGCAGTATTACGCCAAGGGCTGCTATTTCACGCTAGCCGGGCGT
GCGCCGTTTTCGCGCCTGATCTATCCGGTGCCCGAAGCCGCCGGCCTCGGCGTGCACCTGACGATCGACATGGGCGGGCA
AGCCCGCTTCGGCCCGAATGTGCGATGGATCGACGACATCGAATACAGCGTCGACCCCGCTGATGCAGACAGCTTCTATG
ACGAGGTGCGCCGCTACTGGCCGGCGCTCGCCGACGGGGCGTTGCAGCCCGGCTATGCCGGCATCCGTCCGAAGATCAGT
GGCCCGCATGAAGTTGCCGCCGATTTCCGCATCGATGGCCCCGCGGTGCACGGCGTGCCTGGACTGGTTCACCTGTTCGG
CATCGAGTCCCCGGGGCTGACCTCGTCGCTGGCCATCGCCGAACGCGTGGCCGCCGAATTGTCCTGA

Upstream 100 bases:

>100_bases
CGACCACCTGAACGCCGCCGCCGGACGGCTTGGCCTTCGCCTGGTCTGAGACGGTCAGGCGCATATCGAATACAGCATCA
ACCAGAACAAGGGAAAGACC

Downstream 100 bases:

>100_bases
GCCGCGCCCGAGCCGCTACGGAAACTCATCGCGGCACAGATGGCACCCGCGGCAGCAGCGGCCGCCGCAAGACCGAAGGT
ATGCGCGGGGGCGAGCGCCT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MENVDCVVIGAGVVGLAVARALAQNGREVIILEAENAFGTITSARNSEVIHAGIYYPAGSLKAELCVRGKEMLYEYCASH
HVAHQRCGKLIVATSEAQVATLEGIRAKAAANGVHDMQLLTRAEAQALEPQLQCHAALLSPSTGIVDSHGLMTALLGDAE
NAGAMLAVQSPVRRGAITPAGIELEVGSEDGGSTTLLARSVVNSAGLTAPELARRIDGMPEDHIPPQYYAKGCYFTLAGR
APFSRLIYPVPEAAGLGVHLTIDMGGQARFGPNVRWIDDIEYSVDPADADSFYDEVRRYWPALADGALQPGYAGIRPKIS
GPHEVAADFRIDGPAVHGVPGLVHLFGIESPGLTSSLAIAERVAAELS

Sequences:

>Translated_368_residues
MENVDCVVIGAGVVGLAVARALAQNGREVIILEAENAFGTITSARNSEVIHAGIYYPAGSLKAELCVRGKEMLYEYCASH
HVAHQRCGKLIVATSEAQVATLEGIRAKAAANGVHDMQLLTRAEAQALEPQLQCHAALLSPSTGIVDSHGLMTALLGDAE
NAGAMLAVQSPVRRGAITPAGIELEVGSEDGGSTTLLARSVVNSAGLTAPELARRIDGMPEDHIPPQYYAKGCYFTLAGR
APFSRLIYPVPEAAGLGVHLTIDMGGQARFGPNVRWIDDIEYSVDPADADSFYDEVRRYWPALADGALQPGYAGIRPKIS
GPHEVAADFRIDGPAVHGVPGLVHLFGIESPGLTSSLAIAERVAAELS
>Mature_368_residues
MENVDCVVIGAGVVGLAVARALAQNGREVIILEAENAFGTITSARNSEVIHAGIYYPAGSLKAELCVRGKEMLYEYCASH
HVAHQRCGKLIVATSEAQVATLEGIRAKAAANGVHDMQLLTRAEAQALEPQLQCHAALLSPSTGIVDSHGLMTALLGDAE
NAGAMLAVQSPVRRGAITPAGIELEVGSEDGGSTTLLARSVVNSAGLTAPELARRIDGMPEDHIPPQYYAKGCYFTLAGR
APFSRLIYPVPEAAGLGVHLTIDMGGQARFGPNVRWIDDIEYSVDPADADSFYDEVRRYWPALADGALQPGYAGIRPKIS
GPHEVAADFRIDGPAVHGVPGLVHLFGIESPGLTSSLAIAERVAAELS

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=275, Percent_Identity=31.6363636363636, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI87082148, Length=378, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17565764, Length=409, Percent_Identity=30.3178484107579, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24585081, Length=383, Percent_Identity=30.5483028720627, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 38690; Mature: 38690

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVDCVVIGAGVVGLAVARALAQNGREVIILEAENAFGTITSARNSEVIHAGIYYPAGS
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCCCEEEEEEECCCCC
LKAELCVRGKEMLYEYCASHHVAHQRCGKLIVATSEAQVATLEGIRAKAAANGVHDMQLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHCCCHHHHHH
TRAEAQALEPQLQCHAALLSPSTGIVDSHGLMTALLGDAENAGAMLAVQSPVRRGAITPA
HHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCEEEEECCCHHHCCCCCC
GIELEVGSEDGGSTTLLARSVVNSAGLTAPELARRIDGMPEDHIPPQYYAKGCYFTLAGR
CEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCEEEEEECC
APFSRLIYPVPEAAGLGVHLTIDMGGQARFGPNVRWIDDIEYSVDPADADSFYDEVRRYW
CCHHHHEECCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
PALADGALQPGYAGIRPKISGPHEVAADFRIDGPAVHGVPGLVHLFGIESPGLTSSLAIA
HHHHCCCCCCCCCCCCCCCCCCHHHEECEEECCCCCCCCCCEEEEEECCCCCCCHHHHHH
ERVAAELS
HHHHHHCC
>Mature Secondary Structure
MENVDCVVIGAGVVGLAVARALAQNGREVIILEAENAFGTITSARNSEVIHAGIYYPAGS
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCCCEEEEEEECCCCC
LKAELCVRGKEMLYEYCASHHVAHQRCGKLIVATSEAQVATLEGIRAKAAANGVHDMQLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHCCCHHHHHH
TRAEAQALEPQLQCHAALLSPSTGIVDSHGLMTALLGDAENAGAMLAVQSPVRRGAITPA
HHHHHHHCCHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCEEEEECCCHHHCCCCCC
GIELEVGSEDGGSTTLLARSVVNSAGLTAPELARRIDGMPEDHIPPQYYAKGCYFTLAGR
CEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCEEEEEECC
APFSRLIYPVPEAAGLGVHLTIDMGGQARFGPNVRWIDDIEYSVDPADADSFYDEVRRYW
CCHHHHEECCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
PALADGALQPGYAGIRPKISGPHEVAADFRIDGPAVHGVPGLVHLFGIESPGLTSSLAIA
HHHHCCCCCCCCCCCCCCCCCCHHHEECEEECCCCCCCCCCEEEEEECCCCCCCHHHHHH
ERVAAELS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]