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The map label for this gene is mdeA [H]

Identifier: 73540948

GI number: 73540948

Start: 1337164

End: 1338474

Strand: Reverse

Name: mdeA [H]

Synonym: Reut_A1253

Alternate gene names: 73540948

Gene position: 1338474-1337164 (Counterclockwise)

Preceding gene: 73540954

Following gene: 73540947

Centisome position: 35.16

GC content: 67.35

Gene sequence:

>1311_bases
ATGTCTGGTACCCGTTTCGACACGCTGGCGCTGCACGCCGGCGCCGCGCCCGATCCCGCCACGGGGGCCCGGGCCACGCC
CATCCACCTGAGCACCTCGTTCGTGTTCAAGGACAGCGAGCATGCCGCCTCGCTGTTCAACATGGAGCGCGCCGGCCACG
TTTACTCGCGCATTTCCAACCCCACCGTGGCGGTACTGGAAGAACGCATTGCCGCACTGGAAGGCGGCGCGGGCGCGATC
GCGACTGCCTCGGGCCAGGCTGCGCTGCACCTTGCCGTGGCCACGCTGATGGGCGCCGGCTCGCATATCGTTGCGTCCAC
CGCGCTGTATGGCGGCTCGCAGAACCTTCTGCATTACACGCTGCGCCGCTTCGGCATCGAGACCACCTTCGTCAAGCCGC
GCGACATCGACGGCTGGCGCGCCGCCATCCGCCCGAACACGCGACTGCTGTTCGGCGAATCGCTCGGCAATCCGGGCCTC
GATGTGCTGGACATCCCGACGCTGGCGCAGCTCGGCCAGGACAACGGCATTCCGCTGCTGGTCGATTCCACGTTCACCAC
GCCCTACCTGCTGCGCCCGTTCGACCACGGCGCGGGCCTGCTCTACCATTCGGCCACCAAGTTCCTGGGCGGCCACGGCA
CCACCATCGGCGGCGTGCTAGTGGAAGGCGGCACGTTCGATTTCGAGGCCTCCGGGCGCTTCCCGGAACTGTCGGAGCCG
TATGAAGGCTTCCACAACATGGTCTTCACCGAGGAAAGCACGGTCGCTCCGTTCCTGCTGCGCGCGCGCCGCGAAGGCCT
GCGCGACTTTGGCGCCTGCATGAACCCGATGGCCGCGTGGCAACTGCTGCAAGGCGTGGAAACGCTGCCGCTGCGCATGG
CGCGCCATGTCGAAAACACGCGGCGCGTGGTGCAGTTCCTGGCGTCGAACCCGATGGTCGAATCGGTCGCGTATCCCGAA
CTGGAATCGCACCCCGACTACGAACTGGCCAAGCGCCTGCTGCCGCGCGGCTGCGGCGCGGTGTTCAGCTTCAACCTCAA
GGGCAACCGCGAAGCCGGCAAGCGCTTCATCGAAAGCCTGTCGCTGTTCTCGCACCTGGCCAATGTCGGCGACGCCCGCT
CGCTGGTGATCCACCCGGCCTCGACCACGCACTTCCGCATGGATGCCGCCGCGCTGCAGGCGGCCGGTATCACCGAGGGG
ACCATCCGCCTGTCGATTGGCCTCGAAGACGCCGACGACCTGATCGACGACCTCAAGCGCGGCCTGAAGGCTGCGGAGAA
GGCGATGAGCGGCAAGGGAGGGCAAGCCTGA

Upstream 100 bases:

>100_bases
CCGCATAAGTAAAGCACAGGCCGGTTTCCGGGCGGATGGCCCCACCGTCTCACGCACGGTGCTACGATGCAGCCCGGAGA
CAACTCTCGCCCGAATCGCC

Downstream 100 bases:

>100_bases
TGGAACTGAAGATCGCAGGCCAGCCGGCCTACGCCTACACCGGTGGCAAGCCGTTCGACCCCACCCTGCCCTGCGCCGTG
TTCGTGCACGGTGCGCAGAA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSGTRFDTLALHAGAAPDPATGARATPIHLSTSFVFKDSEHAASLFNMERAGHVYSRISNPTVAVLEERIAALEGGAGAI
ATASGQAALHLAVATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIETTFVKPRDIDGWRAAIRPNTRLLFGESLGNPGL
DVLDIPTLAQLGQDNGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLVEGGTFDFEASGRFPELSEP
YEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENTRRVVQFLASNPMVESVAYPE
LESHPDYELAKRLLPRGCGAVFSFNLKGNREAGKRFIESLSLFSHLANVGDARSLVIHPASTTHFRMDAAALQAAGITEG
TIRLSIGLEDADDLIDDLKRGLKAAEKAMSGKGGQA

Sequences:

>Translated_436_residues
MSGTRFDTLALHAGAAPDPATGARATPIHLSTSFVFKDSEHAASLFNMERAGHVYSRISNPTVAVLEERIAALEGGAGAI
ATASGQAALHLAVATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIETTFVKPRDIDGWRAAIRPNTRLLFGESLGNPGL
DVLDIPTLAQLGQDNGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLVEGGTFDFEASGRFPELSEP
YEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENTRRVVQFLASNPMVESVAYPE
LESHPDYELAKRLLPRGCGAVFSFNLKGNREAGKRFIESLSLFSHLANVGDARSLVIHPASTTHFRMDAAALQAAGITEG
TIRLSIGLEDADDLIDDLKRGLKAAEKAMSGKGGQA
>Mature_435_residues
SGTRFDTLALHAGAAPDPATGARATPIHLSTSFVFKDSEHAASLFNMERAGHVYSRISNPTVAVLEERIAALEGGAGAIA
TASGQAALHLAVATLMGAGSHIVASTALYGGSQNLLHYTLRRFGIETTFVKPRDIDGWRAAIRPNTRLLFGESLGNPGLD
VLDIPTLAQLGQDNGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLVEGGTFDFEASGRFPELSEPY
EGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENTRRVVQFLASNPMVESVAYPEL
ESHPDYELAKRLLPRGCGAVFSFNLKGNREAGKRFIESLSLFSHLANVGDARSLVIHPASTTHFRMDAAALQAAGITEGT
IRLSIGLEDADDLIDDLKRGLKAAEKAMSGKGGQA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=35.5450236966825, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=32.9383886255924, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=31.5914489311164, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=34.5238095238095, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789383, Length=432, Percent_Identity=28.0092592592593, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=432, Percent_Identity=30.787037037037, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=32.1513002364066, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=32.311320754717, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=42.6267281105991, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6319307, Length=429, Percent_Identity=33.1002331002331, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=452, Percent_Identity=27.6548672566372, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=26.685393258427, Blast_Score=122, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=27.2727272727273, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=35.4460093896714, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46705; Mature: 46573

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTRFDTLALHAGAAPDPATGARATPIHLSTSFVFKDSEHAASLFNMERAGHVYSRISN
CCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
PTVAVLEERIAALEGGAGAIATASGQAALHLAVATLMGAGSHIVASTALYGGSQNLLHYT
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LRRFGIETTFVKPRDIDGWRAAIRPNTRLLFGESLGNPGLDVLDIPTLAQLGQDNGIPLL
HHHHCCCEEEECCCCCCCHHHEECCCCEEEEECCCCCCCCCEECCCHHHHCCCCCCCEEE
VDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLVEGGTFDFEASGRFPELSEP
EECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEECCEEEECCEEEEECCCCCCCCCCH
YEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENT
HHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHH
RRVVQFLASNPMVESVAYPELESHPDYELAKRLLPRGCGAVFSFNLKGNREAGKRFIESL
HHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
SLFSHLANVGDARSLVIHPASTTHFRMDAAALQAAGITEGTIRLSIGLEDADDLIDDLKR
HHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
GLKAAEKAMSGKGGQA
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SGTRFDTLALHAGAAPDPATGARATPIHLSTSFVFKDSEHAASLFNMERAGHVYSRISN
CCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCC
PTVAVLEERIAALEGGAGAIATASGQAALHLAVATLMGAGSHIVASTALYGGSQNLLHYT
CHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
LRRFGIETTFVKPRDIDGWRAAIRPNTRLLFGESLGNPGLDVLDIPTLAQLGQDNGIPLL
HHHHCCCEEEECCCCCCCHHHEECCCCEEEEECCCCCCCCCEECCCHHHHCCCCCCCEEE
VDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLVEGGTFDFEASGRFPELSEP
EECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCEECCEEEECCEEEEECCCCCCCCCCH
YEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMARHVENT
HHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCHHHHHHHHHHHHHHH
RRVVQFLASNPMVESVAYPELESHPDYELAKRLLPRGCGAVFSFNLKGNREAGKRFIESL
HHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
SLFSHLANVGDARSLVIHPASTTHFRMDAAALQAAGITEGTIRLSIGLEDADDLIDDLKR
HHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
GLKAAEKAMSGKGGQA
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]