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The map label for this gene is yueD [H]

Identifier: 73540773

GI number: 73540773

Start: 1161333

End: 1162151

Strand: Reverse

Name: yueD [H]

Synonym: Reut_A1071

Alternate gene names: 73540773

Gene position: 1162151-1161333 (Counterclockwise)

Preceding gene: 73540774

Following gene: 73540772

Centisome position: 30.53

GC content: 68.86

Gene sequence:

>819_bases
ATGGCCGACCGCAACCGCCAACGACTCACCGCCCGGCGCGGCGGCTCCTCCAACTGGGGCACCATGAGCGAAGCAGACCA
CCTCTACATCATCACCGGCGCCTCTCGCGGGCTCGGCGCGGCACTGACCAATGCGCTGCTAGTGCCGGGCAACCGCCTGG
TTTGCGTGGCGCGCAGCCGCAACCAGGAACTCGAGCGTGTCGCCACCATGAGCGGCGTGCCGGTAGCCTGGCACCTGCAG
GACCTGTCGCAACCCGCGTCGGCCGCGGGCTGGCTGTCCAGCGTACTGGAAAGCCAGGACCCGCCCGCCAGCGTCACGCT
GATCCTGAATGCCGGCGTGGTCGAGCCGATTGGCCCCGTGTCGCGCCTGCAGGAAGGCACGCTGCTGCCGCACCTGCTGA
CGAACCTGGCCACGCCGATGATCATGACCGGCGCGTTCCTCGATCGCACCGAGAAGTTCAACTGCCCGCGCAAGGTGCTC
GCGATCTCGTCCGGTGCGGCACGCCGCCCGATCGAGGGCTGGAGCGCCTACTGCGCCGGCAAGGCCGGGCTGGACATGTT
CATCCGCTCGGTCAACGCCGAGTACGCCCATACGCCGGAGCCGCGCACCGTACGCGCGGTGTCGCTGGCGCCGGGCGTAG
TCGATACCGGCATGCAGGCCACCATCCGCAATGCCGACTTTGCCGGCGTGGAGCGTTTCCGCGACCTGAAGGCGAACCAG
CAGCTCGCCTCGGCGGAGGACACCGCCGGCCGTATCATCGCCTATCTGCATCGCCCGGACTTCGGCAGCACCGAGCTGGA
CGACCTGCGCAATTACTGA

Upstream 100 bases:

>100_bases
GTCCAACACCCAGAAGGTGCTGGACGGCGACCTGGACCCGTTCATCGAGGCCAGCCTCAAGCAGGGACTGTAAGGCCGCT
GCCGTGCCCGCCATGCGGGC

Downstream 100 bases:

>100_bases
AGCCATACTGAACCCGCGCGCGGTACGCCGCGTGCGATCCGACAAGACATCATGACTGAACCCAACCGCGCCCAGGCCGC
ACCGGCCTCTCCGACCGCAG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MADRNRQRLTARRGGSSNWGTMSEADHLYIITGASRGLGAALTNALLVPGNRLVCVARSRNQELERVATMSGVPVAWHLQ
DLSQPASAAGWLSSVLESQDPPASVTLILNAGVVEPIGPVSRLQEGTLLPHLLTNLATPMIMTGAFLDRTEKFNCPRKVL
AISSGAARRPIEGWSAYCAGKAGLDMFIRSVNAEYAHTPEPRTVRAVSLAPGVVDTGMQATIRNADFAGVERFRDLKANQ
QLASAEDTAGRIIAYLHRPDFGSTELDDLRNY

Sequences:

>Translated_272_residues
MADRNRQRLTARRGGSSNWGTMSEADHLYIITGASRGLGAALTNALLVPGNRLVCVARSRNQELERVATMSGVPVAWHLQ
DLSQPASAAGWLSSVLESQDPPASVTLILNAGVVEPIGPVSRLQEGTLLPHLLTNLATPMIMTGAFLDRTEKFNCPRKVL
AISSGAARRPIEGWSAYCAGKAGLDMFIRSVNAEYAHTPEPRTVRAVSLAPGVVDTGMQATIRNADFAGVERFRDLKANQ
QLASAEDTAGRIIAYLHRPDFGSTELDDLRNY
>Mature_271_residues
ADRNRQRLTARRGGSSNWGTMSEADHLYIITGASRGLGAALTNALLVPGNRLVCVARSRNQELERVATMSGVPVAWHLQD
LSQPASAAGWLSSVLESQDPPASVTLILNAGVVEPIGPVSRLQEGTLLPHLLTNLATPMIMTGAFLDRTEKFNCPRKVLA
ISSGAARRPIEGWSAYCAGKAGLDMFIRSVNAEYAHTPEPRTVRAVSLAPGVVDTGMQATIRNADFAGVERFRDLKANQQ
LASAEDTAGRIIAYLHRPDFGSTELDDLRNY

Specific function: Reduces benzil stereospecifically to (S)-benzoin. Can also reduces 1-phenyl-1,2-propanedione, 1,4-naphthoquinone, 1-(4- methyl-phenyl)-2-phenyl-ethane-1,2-dione, 1-(4-fluoro-phenyl)-2- phenyl-ethane-1,2-dione, methyl benzoylformate, p- nitrobenzaldehyde i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4507185, Length=251, Percent_Identity=33.0677290836653, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=220, Percent_Identity=26.3636363636364, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322227, Length=213, Percent_Identity=27.2300469483568, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 29305; Mature: 29173

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRNRQRLTARRGGSSNWGTMSEADHLYIITGASRGLGAALTNALLVPGNRLVCVARSR
CCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCEEECCCCEEEEEECCC
NQELERVATMSGVPVAWHLQDLSQPASAAGWLSSVLESQDPPASVTLILNAGVVEPIGPV
CHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHH
SRLQEGTLLPHLLTNLATPMIMTGAFLDRTEKFNCPRKVLAISSGAARRPIEGWSAYCAG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHCC
KAGLDMFIRSVNAEYAHTPEPRTVRAVSLAPGVVDTGMQATIRNADFAGVERFRDLKANQ
HHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHCCCHHEECCCCHHHHHHHHHHHHHH
QLASAEDTAGRIIAYLHRPDFGSTELDDLRNY
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHCC
>Mature Secondary Structure 
ADRNRQRLTARRGGSSNWGTMSEADHLYIITGASRGLGAALTNALLVPGNRLVCVARSR
CCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCEEECCCCEEEEEECCC
NQELERVATMSGVPVAWHLQDLSQPASAAGWLSSVLESQDPPASVTLILNAGVVEPIGPV
CHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHH
SRLQEGTLLPHLLTNLATPMIMTGAFLDRTEKFNCPRKVLAISSGAARRPIEGWSAYCAG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHCC
KAGLDMFIRSVNAEYAHTPEPRTVRAVSLAPGVVDTGMQATIRNADFAGVERFRDLKANQ
HHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHCCCHHEECCCCHHHHHHHHHHHHHH
QLASAEDTAGRIIAYLHRPDFGSTELDDLRNY
HHHCCCCCCCEEEEEEECCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11745140; 11796169 [H]